Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,159,421 A→T 15.7% I346F (ATT→TTT)  mdtC → multidrug efflux system, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,159,4210AT15.7% 128.1 / 2.7 69I346F (ATT→TTT) mdtCmultidrug efflux system, subunit C
Reads supporting (aligned to +/- strand):  ref base A (23/31);  new base T (9/2);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 2.26e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCAT  >  NC_000913/2159273‑2159566
                                                                                                                                                       |                                                                                                                                                 
ggTTGACAGCACACGGGTAAACCGACCGGCGTTGCAGCAAACCACCCCGGCGGCGATTGCGCTGCAAATTGCCCAGGCTCGCTCCCCCTCCACATGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGa                                                                                                                                                   <  2:1684971/149‑1 (MQ=255)
          agccGGGGAAAATGACCGGAGTCGCAGGATACCATTCCGGCGGGGATTGATCCGCAAATTGCCCAGGATCGCTCCCCCCCCTTTCGCGCTTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTggtggt                                                                                                                                         <  2:74214/147‑1 (MQ=255)
           tCCGGGCAAAATTACCGGAGTTGCCGGACACCATTCCGGCGGCTATTGATCTGCAAAATGCCCAGGATCGATCGCCGCCCACTAGCGCCTCGGTGGAAGTAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATACTGggggtg                                                                                                                                        >  1:1356120/1‑149 (MQ=255)
           tCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAGGTCGAGCAAACGCTGATTATCTCGGTGGCCCCGGTGTTACtgatagag                                                                                                                                        >  2:901589/1‑143 (MQ=255)
           tCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGgtggtg                                                                                                                                        >  2:1671947/1‑149 (MQ=255)
           tCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGggggtg                                                                                                                                        >  1:1344946/1‑149 (MQ=255)
             ggggCAGAATACCGCAGTTGCAGGACACCATTCCGGCGGCTCTTGATCTGCAAATTGCCCAGGAGAGCGCCCCCCCCCTCCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGttt                                                                                                                                     <  2:36593/148‑1 (MQ=255)
             cGGGCAAGATTACCGGAGTGGCAGGACTCGTTTCCGGGGGCGGTTGAGCTGCCAGTGGCCCAGGGTCGCCCCCCCACCTTGTGCGCCTCGCTGGATGGAGTCGTGCAGGCGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGtt                                                                                                                                      <  2:842293/149‑1 (MQ=255)
              gggCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGGGgcgtct                                                                                                                                     >  1:728359/1‑147 (MQ=255)
               ggCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGCTTCTGGTGGTGtttt                                                                                                                                    >  1:29641/1‑149 (MQ=255)
                 cAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCGCCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTCTCTCGGTGGCGCGGCTGATTCGGGTGGTGgttttg                                                                                                                                  >  1:979783/1‑148 (MQ=255)
                 cAAAATTACCGGAGGTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTa                                                                                                                                  <  1:795371/149‑1 (MQ=255)
                   aaaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCCCCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTAtt                                                                                                                                <  2:100291/149‑1 (MQ=255)
                    caTTACCGGAGTCGCAGGAATCCATCCCGGCGGCGATTGCTGTGCAAATTGCCCAGGATCGCTCGTCCACCATTCGCGGCTCGCTGGAATAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTc                                                                                                                               <  2:594901/148‑1 (MQ=255)
                    aagtACCGGAGTTGCTGGAAACCAGTCCGGCGGCGGTTGATGTGCAAATTGCCCGGGATCGCTGCCCCACCTTTCGGGCGTCGCTGGAAGGAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTc                                                                                                                               <  2:584892/146‑1 (MQ=255)
                    aaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCCGGTGTCTCTGAAGGAGATTATATTc                                                                                                                               >  2:790400/1‑149 (MQ=255)
                        aCCGGGGTTGCATGATACCATTCCGGCGGCTATCGATCCGCAAATTGCACAGGAGCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAGCGCTGGTTATCTCGGTGGCGCTGGTCATTCTGGTGGTGTTTTTATTCCTgc                                                                                                                           <  2:966578/149‑1 (MQ=255)
                            gAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCTCCACCTGCGCGCGTCCCTGGCAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTTTGGCGGTGGTTTTCTTCTTGCACTc                                                                                                                       >  2:655619/1‑149 (MQ=255)
                               ttGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACAATTCGCGCCTCGCTGGACGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGAATCTGGTGGTGTTTGTATTCCTGCGCTCggg                                                                                                                    >  1:509570/1‑149 (MQ=255)
                                tGCACGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATGCGCGCCTCGCTGGAAGAAGGCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGt                                                                                                                   <  1:328015/149‑1 (MQ=255)
                                   aGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTcgc                                                                                                                >  2:1196212/1‑149 (MQ=255)
                                   aGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCCGGTGATCCTGGTGGAGTTTTTATTCCTGCGCTCGGGTcgc                                                                                                                >  2:1232258/1‑149 (MQ=255)
                                     gAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCCGTGGTGTCTTTGGTGGTGTTTTTATTCCTGGGCTCGGGTCgcgc                                                                                                              >  2:725575/1‑149 (MQ=255)
                                     gAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAGCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTGTTCCGGCGCTCGGGTggcgg                                                                                                              >  1:285456/1‑148 (MQ=255)
                                      caaCCATCCCGGCGGCGACTGCTCTGCAAATTGCCCAGGATCGCTCCCCCGCCGTTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGcc                                                                                                             <  1:1758958/148‑1 (MQ=255)
                                               cggcggCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCAACTattatt                                                                                                    >  1:131462/1‑149 (MQ=255)
                                                       aTTGATCTGCAAATTGCCCAGGATCGCCCCCCACCGATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCCCGTGACTCTGGAAGTGAATTTATTCCTGCCCTCGGGTCGCGACACTAGTATTCCCGCCGt                                                                                            >  2:1748762/1‑149 (MQ=255)
                                                           aTCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGTTGGTGCTGGTGAAAATATAGCAAAGCACATCCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCg                                                                                        >  2:1737934/1‑149 (MQ=255)
                                                             cTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCCCTATTATTCCCGCGGTTTCGGt                                                                                      >  1:4215/1‑149 (MQ=255)
                                                               gCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGTGGCGGTGATATTAAAGGAGAATTTATACCTGCGCTAGAGTCGCGCCACTATTATTGCCGACGTTTAGGTGc                                                                                    >  2:1413297/1‑149 (MQ=255)
                                                               gCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGGCGGTGTCTCTGGTGATGATATTCTTCCTGCGCTCGGGTCGCGCCACCATTATTTCCGCCGTTTCGGTga                                                                                    >  2:938298/1‑148 (MQ=255)
                                                                    ttGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCCCTCGTATCTCTGGAAGAGATATTATTACTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGtt                                                                               >  2:750472/1‑149 (MQ=255)
                                                                     tGCCCAGGATCGCCCCCCCACCTTTCGCGCCCCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGttt                                                                              <  1:1431671/149‑1 (MQ=255)
                                                                     tGCCCAGGATCGCCCCCCCACCAGTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGttt                                                                              <  1:1058515/149‑1 (MQ=255)
                                                                        ccgggctCGCTCCCCCACCATGCGCGCCGCGCTGGAAGAAGTCGAGCAACCGCTGATGATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTTGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGc                                                                           <  2:754711/143‑1 (MQ=255)
                                                                          aGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGTCCCGGTGTCATTGGAGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTACTCCCGCCGTGTCGGTGCCGGTTTCGccg                                                                         >  2:1789564/1‑147 (MQ=255)
                                                                            gATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCGTGCGCTCGGGTCGCGCCACTATTATTGCCGCCGTTTCGGTGCCGGTTTCGCTGAt                                                                       >  1:376444/1‑149 (MQ=255)
                                                                             accGCCCCCCCACCATTCGCGCCCCGCTGGAAGAAGTCCAGCAAACCCTGATTATCTCGGTGGCGCTCGTGATTCTGGTGGGGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGAtt                                                                      <  2:1446818/147‑1 (MQ=255)
                                                                             aTCGCTCCCCCAACCTTCGCCCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGAtt                                                                      <  1:838549/149‑1 (MQ=255)
                                                                                  tCCCCCGCCACTCGCGCCTCGCGGGAAGGAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTAc                                                                 <  1:680943/149‑1 (MQ=255)
                                                                                  ccccccACCATTCGCCCCTCGCTGGACGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTAc                                                                 <  1:1383487/148‑1 (MQ=255)
                                                                                     cccACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCCGCCGTAGCTTTTGAAGAAAAATTATTCCTGCCCTCGGGTCTCGCCACTATTATGCACGCCGTTTCGGTGCCGGTTTCGCGGATTGGTACGtt                                                              >  2:831621/1‑149 (MQ=255)
                                                                                             aTTCGCGCCTGGCCGGAAGAAGCCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTgcggc                                                        <  1:981108/149‑1 (MQ=255)
                                                                                              ttCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTgcggcg                                                       >  1:1116997/1‑149 (MQ=255)
                                                                                              ttCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCCGCCGTATCATTAAAAAAGAAATTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGGTTCGGTGCCGGTTTAGCTGAATGGTACCTTTgcggcg                                                       >  2:598652/1‑149 (MQ=255)
                                                                                                    ccTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTAc                                                 <  1:1671947/149‑1 (MQ=255)
                                                                                                     cTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCCGCCGTATCACTGAAAAAAAAATTATTCCTGCGCTCGGGTCGCGCCACTCTTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTCGGTTTGCGGCGACGGAcc                                                >  2:670162/1‑149 (MQ=255)
                                                                                                     cTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCCGCCGTAACACTAAAAAAGAAATTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGATTCGCAGATTGGTACGTTTGCGGCGATGTAcc                                                >  2:670366/1‑149 (MQ=255)
                                                                                                     cTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCCCCCGTGACTCTGGAGGAGTTTTTATTCCTGCGCTCGGGTCGAGCCACTATTATCACCGCCGTTTCGCTGCCGGGTTCGCTGATTGGTACGTTTGCGGCGATGTAct                                                >  2:374977/1‑148 (MQ=255)
                                                                                                            gaagaaGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGGGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGGACGTTTGCGGCGATGTACCTGTGCgg                                         >  1:756167/1‑149 (MQ=255)
                                                                                                             aagaagTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGa                                        >  1:1174960/1‑149 (MQ=255)
                                                                                                               gatgTCGGGCCAGCGCTGGTTACCTCGGTGGCGCTGGTGCTTCGGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGAtt                                      <  2:1613362/146‑1 (MQ=255)
                                                                                                                   tCGGGCAAACGCTGATTATCTGGGTGGCGCTGGTGATTCTGGTGGTGTTCTTATTCCTGCGCTCGGGTCGCGCCACTATTATCCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGt                                  <  1:655619/149‑1 (MQ=255)
                                                                                                                    cgcgCAGACGCCGATTATCGCGGTGGCGGTGGTCATTCTGGGGGTGTTTTTATCCCGGCGGCCGGGTCCCGCCCCTATGGTGCCTGCCGTTTCGGTGCCGGTTTCGCGGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGtc                                 <  2:1069988/146‑1 (MQ=255)
                                                                                                                     gAGCAAACGCTGATTATCTCGGTGGCGGCGGTATCACTGGAAATGAAATTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGtct                                >  2:1640869/1‑149 (MQ=255)
                                                                                                                       ccgaccGCTGATTATCTCGGTGGCGCTGGTGGTTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTACTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCa                              <  2:319542/144‑1 (MQ=255)
                                                                                                                         catcGCTGATTATCTTGGTGGCGCGGGTGATTCTGGTGGTGTGTTTATTCCTGCGCTCGGGTGGCGCCCCTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTCCCTGTGCGGATTCAGTCTCAAt                            <  2:1778614/146‑1 (MQ=255)
                                                                                                                          agcGCTGCTTATCTCGGGGGCGCTGGCGATTCTGGGGGTGTGTTCATTCCTGCCGTCGGGTCGCGCCACTATTATTCCCGCGGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATa                           <  2:728359/147‑1 (MQ=255)
                                                                                                                            cgtggATTATCTCGGTGGCCCTGGGGATTCTGGCGGTGTTTTGACTCCTGCGCTCGGGTTGCGCCACTATTATTCCCGCCGTTTCGGTCCCCGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAAc                         <  2:1577444/145‑1 (MQ=255)
                                                                                                                            cGCTGATTATCTCGGTCGCGCTCGTGTTTCTGGTGGTGTCTTCATTCCTCCGCTCGGGTCGCGCCACCATGTTTCCCGCCGTTTCGGTCCCGGTTTCGCCGATCGGTACGCTCGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAAc                         <  2:885322/149‑1 (MQ=255)
                                                                                                                             gCTGATTGACCCGGGGGCGCTGGTGATTCTGGTGGTGTTTTTATCCCTGCGCCCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAAcc                        <  2:44879/149‑1 (MQ=255)
                                                                                                                             gCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAAcc                        <  1:1196212/149‑1 (MQ=255)
                                                                                                                               tgctttgctcGGGGGCGTTGGTGATTCTGGGGGTGTTTTTATTCCTGCGCTCGGGTCGGGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCtt                      <  2:736280/142‑1 (MQ=255)
                                                                                                                               tGATTAGCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGCCTACATGTACAATCAGTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGCTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCtt                      <  1:1779537/149‑1 (MQ=255)
                                                                                                                                 aTTATCTCGGGCGCGCTTGTGATTCTGGGGTTGTTTTTACTCCCCCCCTCGGGTCGCGCTACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGCCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTc                    <  2:826279/149‑1 (MQ=255)
                                                                                                                                   tATCTCGGTGGCGCTGGCGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGt                  <  1:289/149‑1 (MQ=255)
                                                                                                                                        cGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATg             <  1:1737934/149‑1 (MQ=255)
                                                                                                                                            ggCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGgcgc         >  2:1120117/1‑149 (MQ=255)
                                                                                                                                              acgcgGGTGTTTCTGGTGGTGTTTTTCTTCCTGCGCTCGGGTCGCGCCACTATTGTTCCCGCCGGTTCGGTGCCGGTTTCGCTGTTTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCt        <  2:1208128/148‑1 (MQ=255)
                                                                                                                                                    gTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACGAt  >  1:1554807/1‑149 (MQ=255)
                                                                                                                                                       |                                                                                                                                                 
GGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCAT  >  NC_000913/2159273‑2159566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: