BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,577,084	C→T	7.0%	V170I (GTT→ATT)	gntK ←	gluconate kinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,577,084	0	C	T	7.0%	335.6 / 5.6	119	V170I (GTT→ATT)	gntK	gluconate kinase 2
Reads supporting (aligned to +/- strand): ref base C (51/58); new base T (7/2); total (59/60)
Fisher's exact test for biased strand distribution p-value = 9.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

AAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGG  >  NC_000913/3576936‑3577232

aaaaGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAAc                                                                                                                                                      <  1:1118271/149‑1 (MQ=255)

aaaaGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAAc                                                                                                                                                      <  1:979571/149‑1 (MQ=255)

aaaaGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAAc                                                                                                                                                      <  2:1156610/149‑1 (MQ=255)

aaaaGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAAc                                                                                                                                                      <  1:1537116/149‑1 (MQ=255)

aaaaGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAAc                                                                                                                                                      <  1:1481551/149‑1 (MQ=255)

  aaGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATAGGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCt                                                                                                                                                    >  2:1391890/1‑149 (MQ=255)

  aaGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTGCTGCTGTTGAAACAAGCGTTAATGTCGTCACTCCTTATTTGCCTTTTTTAATAACCt                                                                                                                                                    >  2:491287/1‑149 (MQ=255)

  aaGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCt                                                                                                                                                    >  2:839359/1‑149 (MQ=255)

  aaGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCt                                                                                                                                                    >  2:1713177/1‑149 (MQ=255)

       cAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATg                                                                                                                                               >  2:1633947/1‑149 (MQ=255)

        aGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGGGCATACGCGCCTTCATGACTAAAAAAAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATgg                                                                                                                                              >  1:1173691/1‑149 (MQ=255)

          cccccATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTg                                                                                                                                            <  2:1685366/149‑1 (MQ=255)

          cccccATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTg                                                                                                                                            >  2:1590824/1‑149 (MQ=255)

          cccccATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTCTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTg                                                                                                                                            <  2:1340428/149‑1 (MQ=255)

           ccccATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTACTGTAGTCACTACTTCTTTGCCTTTTTTACTACACTCAATGGTgt                                                                                                                                           >  2:155727/1‑148 (MQ=255)

            cccATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTTAATCAATCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCt                                                                                                                                          <  2:657045/149‑1 (MQ=255)

              cacggACACCACCATTAAAGCCAGGAAAGCGTCCATACGCGCCTTCATCACTAAAAACAGCAGCAGTAAAACAGACCCTACTCCTGTTAAACCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTg                                                                                                                                        <  2:1585704/146‑1 (MQ=255)

              caagaacacCACCCTTCAAGCCAGAAAAGCTTTCACCCCCACCTTCAGGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTg                                                                                                                                        <  1:823791/144‑1 (MQ=255)

                  gACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCcac                                                                                                                                    <  1:142993/149‑1 (MQ=255)

                   acacCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTCTTTGCCTTTTTTAATAACCTCAATGGTGCTTGCcaca                                                                                                                                   >  1:684903/1‑149 (MQ=255)

                   acacCACCATTAAAGCCAGGAAAACGTGCATACGCGCCATCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCcaca                                                                                                                                   >  1:971033/1‑149 (MQ=255)

                       caccaTTAAAGCCCGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGCTAACCATGGAACCTCTTATTTGCCTTTTTTAATAACCTCAATGGGGCTTTCCACAacac                                                                                                                               >  1:1238908/1‑149 (MQ=255)

                       caccaTTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAACCAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAacac                                                                                                                               >  2:280680/1‑149 (MQ=255)

                            ttAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAGCAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCACTGGTGCTTGCCACACCACCTTcc                                                                                                                          >  2:1137129/1‑149 (MQ=255)

                             tAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCa                                                                                                                         <  1:224682/149‑1 (MQ=255)

                              aaaGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAg                                                                                                                        <  1:739649/149‑1 (MQ=255)

                                aGCCAGGAAAGCGTGCATACGCTCCTTCATGACTAAAAACAGCAGCAGTAAAACAGATCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCTACAACACCTTCCAGCg                                                                                                                      <  1:1146841/149‑1 (MQ=255)

                                aGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCg                                                                                                                      <  1:438927/149‑1 (MQ=255)

                                aGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCg                                                                                                                      <  1:88815/149‑1 (MQ=255)

                                 gCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTACTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAAAACCTTCCAGCgg                                                                                                                     >  1:1206483/1‑149 (MQ=255)

                                  ccAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGt                                                                                                                    <  2:979875/149‑1 (MQ=255)

                                     ggAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGa                                                                                                                 <  2:1743446/149‑1 (MQ=255)

                                     ggAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGa                                                                                                                 <  1:770319/149‑1 (MQ=255)

                                      gAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGAt                                                                                                                >  1:89960/1‑149 (MQ=255)

                                       aaaGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGTTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATc                                                                                                               >  2:699997/1‑149 (MQ=255)

                                         agtgTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGa                                                                                                             <  2:1695259/146‑1 (MQ=255)

                                           cGTGCATCCGCGCCTTCATGACTAAAAGCAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGata                                                                                                           <  1:439981/149‑1 (MQ=255)

                                           cGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGata                                                                                                           <  2:49903/149‑1 (MQ=255)

                                           cGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTACCACAACACCTTCCAGCGGTTGATCGcta                                                                                                           >  1:861150/1‑149 (MQ=255)

                                            gTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGatat                                                                                                          >  2:211712/1‑149 (MQ=255)

                                            gTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGatat                                                                                                          <  1:989773/149‑1 (MQ=255)

                                            gTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGatat                                                                                                          >  1:1144658/1‑149 (MQ=255)

                                             tGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCATTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATc                                                                                                         <  1:353946/149‑1 (MQ=255)

                                             tGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTGATTTGCCTTTTTTAATAACCTCAATGATGCTTGCCACAACACCTTCCAGCGGTTGATCGAtctc                                                                                                         >  1:87434/1‑149 (MQ=255)

                                              gCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATcc                                                                                                        <  2:23529/149‑1 (MQ=255)

                                               cATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTCACTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATcca                                                                                                       <  2:1552821/149‑1 (MQ=255)

                                                    gcgcCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATccaccacc                                                                                                  >  1:1505101/1‑149 (MQ=255)

                                                    gcgcCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATccaccacc                                                                                                  >  1:573308/1‑149 (MQ=255)

                                                    gcgcCTCCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATccaccacc                                                                                                  <  1:132460/149‑1 (MQ=255)

                                                     cgcCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATccaccacca                                                                                                 <  1:924646/149‑1 (MQ=255)

                                                        cTTCATGACTAAAAACAGCAGAAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTa                                                                                              <  1:16429/149‑1 (MQ=255)

                                                          tCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCAAAACACCTTCCAGCGGTTGATCGATATCCACCACCCGTCCa                                                                                            >  1:201740/1‑149 (MQ=255)

                                                                  aaaaaCAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTc                                                                                    <  2:931590/149‑1 (MQ=255)

                                                                     aaCAGCAGCAGTAAAACTGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTc                                                                                 <  2:95875/149‑1 (MQ=255)

                                                                         gcagcagTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCCCCACCAGTACATCGGTTTCGTCCGCa                                                                             >  1:613603/1‑149 (MQ=255)

                                                                                tAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTTTTTTTTTTTTTTTTACTTGCAACAACACCTTCCAGCTGTTGATCGATTTCCTGCACATATACAGCGTTTCCGTCCGCACCTggac                                                                      >  2:445636/1‑147 (MQ=255)

                                                                                tAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTATTTTTTTTAATGGTGCTTGCCACCACCCCTTCCAGCGGTTGATCGATATCCACCAACAGTACCTCGGTTCAGTCCGCACCCgccc                                                                      >  2:1155159/1‑146 (MQ=255)

                                                                                tAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCt                                                                      >  1:790254/1‑149 (MQ=255)

                                                                                tAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGCCCGCACCCGGCt                                                                      >  1:119733/1‑149 (MQ=255)

                                                                                    aCAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTg                                                                  <  1:622210/149‑1 (MQ=255)

                                                                                      aGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCCCTACCAGTACATAGGTTTAGTCCGCACCCGGCTCCTCCa                                                                >  2:365175/1‑149 (MQ=255)

                                                                                          ccTACTGCTGTTAAAACAAGCGTTAATGTAGTCCCTACTTATTTGCCTTTTTTACTAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGACCCCGGCTACTGCAGCgc                                                            >  1:897620/1‑148 (MQ=255)

                                                                                            tACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGttt                                                          <  1:1713177/149‑1 (MQ=255)

                                                                                             aCTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATGTCCACCACAAGGCCATAGGTTTCTTCCGCACCCGGCTCCTGCAGCGTTTc                                                         >  1:338518/1‑149 (MQ=255)

                                                                                               tgctgTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGTTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCCCCAGTACATCGGTTTCGTCCGCACTCGCCTCCTGCAGCGTTTCac                                                       >  2:869126/1‑148 (MQ=255)

                                                                                                gctaTTAAAGCAATAGCTAATGGAGTAATTATTTATTTGCTTTTGTGAATTATCTCTATGTGGCTGTCGACATCACCTTCCAGCGGTTTATCGTTATCCACCGCCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCaaa                                                      <  2:1709036/149‑1 (MQ=255)

                                                                                                 ctgTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTACTAACCTCAATGTTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGAAGCGTTTCAGAc                                                     >  1:132106/1‑149 (MQ=255)

                                                                                                 ctgTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAAc                                                     <  1:839359/149‑1 (MQ=255)

                                                                                                   gTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACCTCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTg                                                   <  1:273291/149‑1 (MQ=255)

                                                                                                   gTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTg                                                   >  1:629662/1‑149 (MQ=255)

                                                                                                   gTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTg                                                   <  1:1432528/149‑1 (MQ=255)

                                                                                                   gTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGATGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTg                                                   >  1:1548155/1‑149 (MQ=255)

                                                                                                    ttAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTTTTTTTTTTTATGGTGCTTGCCACAACACCTTTTAGCGGTTGTTCGTTATCCACTCCCGGTGCATCGGTTGCGTCCGCATACGGCTCGTGCATCGTTTTCAACTgt                                                  >  2:306990/1‑148 (MQ=255)

                                                                                                    ttAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTTTTTTTTTTTATGGTGCTTGCCACAACACCTTTTAGCGGTTGATCGATGTCCACCACCGGTACATCGGTTTCGTCTGTGCCAGCTTCCTGCAGTGTTTCAAACtct                                                  >  2:306567/1‑147 (MQ=255)

                                                                                                    ttAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCATTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGc                                                  >  2:1506298/1‑149 (MQ=255)

                                                                                                      caaaCAATCGTTAATGTAGTCCCTACTTACTTGCCTTTTTTAATAACCTCAATGGTGCTTGTCACAACACCCTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGt                                                <  2:543516/148‑1 (MQ=255)

                                                                                                      aaaaCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGt                                                >  1:511064/1‑149 (MQ=255)

                                                                                                        aaCAGGCGTTAATGTAGTCGCTACTTAGTTGCCTTTTTTAATAACCTCAGTGGTGCTTGCCACCACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCa                                              <  2:1504905/149‑1 (MQ=255)

                                                                                                        aaCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCa                                              <  2:1487801/149‑1 (MQ=255)

                                                                                                         aCAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCAc                                             <  1:1674674/149‑1 (MQ=255)

                                                                                                          cAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCAcc                                            <  1:665529/149‑1 (MQ=255)

                                                                                                            aGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAATCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCaa                                          <  1:280680/149‑1 (MQ=255)

                                                                                                                 tagtttcgTCACTACTTATTTTCCTTTTTCAACAACCTCAATGGGGCTTGCCACGACACCTTCCAGCGTTTGATCGATATCCACCACCAGTGCATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACAttt                                     <  2:1218551/142‑1 (MQ=255)

                                                                                                                   aTGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCTGCGTTTCAAACTGCGTCACCAACATTTgg                                   <  1:1101200/149‑1 (MQ=255)

                                                                                                                   aTGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAAGGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTgg                                   <  2:722569/149‑1 (MQ=255)

                                                                                                                            ctactTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCCCCCGGCTCCTGCAGCGTTTCACACTGCGTCACCAACATTCGGGTTTTAAAg                          >  2:7600/1‑149 (MQ=255)

                                                                                                                            ctactTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAg                          >  1:395135/1‑149 (MQ=255)

                                                                                                                                 tATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAGAAATg                     <  1:132104/149‑1 (MQ=255)

                                                                                                                                 tATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAGAAATg                     >  1:285071/1‑149 (MQ=255)

                                                                                                                                    ttGGCTTTTTGAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAGAAATGGcc                  <  2:810075/149‑1 (MQ=255)

                                                                                                                                         ttttttAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAGAAATGGCCTTTgc             <  1:1633947/149‑1 (MQ=255)

                                                                                                                                         ttttttAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAGAAATGGCCTTTgc             >  1:737733/1‑149 (MQ=255)

                                                                                                                                          tttttAATCACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAGAAATGGCCTTTgcg            <  1:448879/149‑1 (MQ=255)

                                                                                                                                          tttttAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCTGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAGAAATGGCCTTTgcg            >  1:652028/1‑149 (MQ=255)

                                                                                                                                          tttttAATAACCGCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAGAAATGGCCTTTgcg            <  1:698362/149‑1 (MQ=255)

                                                                                                                                            tttAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTGGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAGAAATGGCCTTTgcgcg          >  1:1351801/1‑149 (MQ=255)

                                                                                                                                               aataaCCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCACACTGCGTCCCCAACATTTGGGTTTTACAGGAAGGGCCTTTGCGCGCtt       >  1:197219/1‑149 (MQ=255)

                                                                                                                                                 taaCCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTc     >  1:1430435/1‑149 (MQ=255)

                                                                                                                                                    ccTCAAGGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAgg  <  1:290766/149‑1 (MQ=255)

                                                                                                                                                    ccTCAAGGGTGCTGGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAgg  <  2:127091/149‑1 (MQ=255)

AAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCACAACACCTTCCAGCGGTTGATCGATATCCACCACCAGTACATCGGTTTCGTCCGCACCCGGCTCCTGCAGCGTTTCAAACTGCGTCACCAACATTTGGGTTTTAAAGAAATGGCCTTTGCGCGCTTTCAGG  >  NC_000913/3576936‑3577232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`