Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 349,673 | G→C | 5.1% | intergenic (+101/‑339) | prpB → / → prpC | 2‑methylisocitrate lyase/2‑methylcitrate synthase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 349,673 | 0 | G | C | 5.1% | 345.4 / 3.7 | 143 | intergenic (+101/‑339) | prpB/prpC | 2‑methylisocitrate lyase/2‑methylcitrate synthase |
Reads supporting (aligned to +/- strand): ref base G (65/66); new base C (7/4); total (73/70) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.33e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 144 total aligned reads displayed.
TACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATC > NC_000913/349525‑349818 | tACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACgg > 1:442713/1‑149 (MQ=255) cGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTCCGAGGTGCACTGAACTGTAGGTCGGATAAGCCGGac > 2:787696/1‑148 (MQ=255) agagAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGCATGGc > 2:1299755/1‑149 (MQ=255) agaAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGt > 2:585015/1‑149 (MQ=255) agaAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGt < 1:1546861/149‑1 (MQ=255) gaAGCTCGACAACCTGTTTGCCCGGAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCCGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTc > 1:1348099/1‑149 (MQ=255) aGCTCCACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGc < 1:1403356/149‑1 (MQ=255) tCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGCTGCCTGATGCGTCGCTGACGGGACTTATCAGGCCTACGGGGTGCACTGAACGGGAGGTCGGATAAGACGGGTGGGGACGCATc > 2:496857/1‑149 (MQ=25) tCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATc < 1:790322/149‑1 (MQ=255) cGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTGCCATcc > 2:444140/1‑149 (MQ=255) agACAACCTGTTTGCCCGCAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATcc < 1:285847/148‑1 (MQ=255) gACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGAACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCg > 1:1777512/1‑149 (MQ=255) aCAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCgc > 1:571023/1‑148 (MQ=255) aCAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGa > 1:554265/1‑149 (MQ=255) cAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGAc > 2:974344/1‑149 (MQ=255) cAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGAc > 1:430592/1‑149 (MQ=255) aaCCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCTCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGAGGGCGTCGCATCCGACa > 2:82189/1‑149 (MQ=255) aaCCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGCACTGTAGGTCGAATAAGACGGCTGGCGTCCCATCCGACa > 1:1396790/1‑149 (MQ=255) aCCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACaa < 2:174077/149‑1 (MQ=255) gcTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGACGCCTGATGCTGCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTCGGTCGGATAAGACGGATGGCGTCGCATCCGACAAc < 2:1549124/148‑1 (MQ=255) ccTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAAc < 1:985680/149‑1 (MQ=255) tttGCCCGTAGCCAGGTGAAATAAATAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGAt > 1:906075/1‑149 (MQ=255) tttGCCCGTAGCCAGGTGAAATAAAGAACGCCCGGTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATGAGACGGATGGCGTCGCACACGGCAACCGaa > 1:905218/1‑148 (MQ=21) tttGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGGCGGATAAGACGGATGGCGTCGCATCCGACAACCGAt > 2:642418/1‑149 (MQ=255) ttGCCCGTAGCCAGGTGAAATAATAAACGCCCGTTGATAGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGCTAAGACGGATGGCGTCGCATCCGACAACCgcgg > 1:973571/1‑146 (MQ=255) ttGCCCGTAGCCAGGTGAAATAAAAGACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATg > 1:1080280/1‑149 (MQ=255) cGTAGCCAGGTGAAATAAAAGACGCCCGTTGATTGTATTCGGCAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGa < 2:862270/149‑1 (MQ=255) cGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGCTAAGACGGATGGCGTCGCATCCCACAACCGATGCCGGa > 1:1350066/1‑149 (MQ=255) gcAGGTGAAAGAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATgcgc < 2:98436/148‑1 (MQ=255) ccAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGCATGGCGTCGCATCCGACACCCGATGCCTGCTgcgc > 2:12761/1‑149 (MQ=255) catgTGAAATAAAAAACGCCCGTTGATCGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTCTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGcgcc < 1:1032282/146‑1 (MQ=255) cAGGTGAAATAAAAAACGCCCGTTGGTTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCGGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGcgcc < 2:418358/149‑1 (MQ=255) ggTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGGCTTATCAGGCCTACGGGGTGGACTGAACTGTAGGTCGGATAAGACGGATGGGGTCGCATCGGACGGCCGAGGCGTGATGcgccgg > 1:2460/1‑148 (MQ=18) gTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGGTGCCTGATGCGCCGCt > 1:1015810/1‑149 (MQ=255) aaTAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACg < 1:1436027/149‑1 (MQ=255) aTAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGt > 1:1177075/1‑149 (MQ=255) aaaaaaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACACCCGATGCCTGATGCGCGGCTGACGTGa > 2:1803546/1‑149 (MQ=255) ccGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGTGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGGTGCCTGATGCGCCGCTGACGTGACTTAt < 1:1180743/148‑1 (MQ=255) aCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTAt < 1:1663531/149‑1 (MQ=255) cGCCCGGTGGTTGTATGCGACAGCCGATGCCTGATGCGTCGCTGGCGCGAGTTATCATGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCCTCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATc < 2:1201214/149‑1 (MQ=17) gtccGTTGACTGTATTCGACAGCCGAGCCCTGGTGCGTCGCTGCCGCGACTTATCAGGCCTACGAGGTGCACTAAACTGGAGGTCGGATAATACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa < 2:554723/147‑1 (MQ=17) gctcGTTGATTGCATTCTACAGCCGAGGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGCCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa < 2:554724/146‑1 (MQ=17) ccGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTACGGCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCGTGATGCGCCGCTGACGTGGCTTATCAgg > 1:1785970/1‑149 (MQ=17) aTTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTGTCAGGCCTACg > 1:956171/1‑149 (MQ=255) ttGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCGTACGACAACCGATGCCTGCTGCGCCGCTGCCGTGACTTGTCAGGCCAACgg > 1:1594917/1‑149 (MQ=17) ttGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGCATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACgg > 2:1403477/1‑149 (MQ=255) tggaTTCGACAGCCGATGCCTGATGCGTCGCTTACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg < 1:283912/146‑1 (MQ=255) tGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg < 1:1440754/149‑1 (MQ=255) tGTATTCGACAGCCGAGGCCGGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg < 2:479238/149‑1 (MQ=255) ggtttCCACAGCCGATGCCTGAGGCGTCGCTCACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACgggg < 2:1277171/146‑1 (MQ=255) gaTTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAATCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGt < 1:484708/148‑1 (MQ=255) cGACAGCCGTTGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCAc < 1:891117/149‑1 (MQ=255) gATGCCGTATGCGTCGCTGACGCGACTTATCAGGCCTGCGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATATCGCGTTCGGGATGAAATGAACTGt < 2:400094/149‑1 (MQ=14) tGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAg < 2:1339486/149‑1 (MQ=255) ccTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGt > 1:889483/1‑149 (MQ=255) tGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGGCATGAACTGTAGGTCg > 2:1261281/1‑149 (MQ=255) tGATGCGTCGCTGACGCGACTTAGCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCg < 1:682805/149‑1 (MQ=255) tGATGCGTCGCTGACGCGACTTAACAGGCCTACGAGGTCCACTGAAGTGTAGGTCGGATAAGACGAATGGCGGTGCATCCGACAACCGATGACTGATGCGCCGCTGACGTGGCTTATAAGCCAAAGGTGTTGCACTGAGCTATGGGtgg > 2:1355568/1‑147 (MQ=255) agATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGCCGGATAAGCCGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCg < 1:705460/148‑1 (MQ=255) gTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACCAAGCAGGCCCACGGGGGGCACGGAACCGTAGGGGGGGTAAg > 1:126270/1‑149 (MQ=14) tCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGa > 1:1730241/1‑149 (MQ=255) tCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGa > 1:303590/1‑149 (MQ=38) gCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACg < 2:537838/149‑1 (MQ=38) cTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGc > 2:1622349/1‑149 (MQ=17) aCGCGACTGATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATa < 2:1812864/149‑1 (MQ=255) gACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCCATGAACTGTCGGTCGGATAGAACGCATAGCGt > 2:1133595/1‑149 (MQ=11) gACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGt > 1:1243637/1‑149 (MQ=9) tctgaTCAGGCCTACGAGGGGCACTGAACGGTATGTCGGATAAGACGGATGGCGTCGCATCCGACAACCTATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTTCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc < 2:1463787/145‑1 (MQ=14) aCTTATCGGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc < 2:1665353/149‑1 (MQ=255) aCTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGGGACTTATCAGGCCAACGGGGGGCACTGAACGGTAGGTCGGATAAGACGCATAGCGTc > 2:474770/1‑149 (MQ=255) ttATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATCGCGTccc > 2:128174/1‑147 (MQ=255) cAGGCCTACGAGTTGCACTGGACCGTAGGGCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATcc < 1:490179/149‑1 (MQ=255) aGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGAGTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTTGGATAAGACGCATAGCGTCGCATCCg > 1:10695/1‑149 (MQ=255) aGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCg > 2:850035/1‑149 (MQ=255) aGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTAGCAGGCCTACGGGGTGCACCGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCg > 1:70706/1‑149 (MQ=255) ggCCTACGAGGTGCCCTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGa < 2:942326/149‑1 (MQ=255) tACGAGGTGCACTTAACTGGCGGTCGAGTAAGCCGCTGTGGGTCGCCTCCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAAc < 1:755505/149‑1 (MQ=11) tACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGGGCACTGAACTGTAGGCCGGCTAACCAGAATAGCGTCGCATCCGACAAc > 1:328396/1‑149 (MQ=14) gAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATACGACGCATAGCGTCGCATCCGGCAAccca > 2:1418001/1‑147 (MQ=255) gAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGa < 1:963632/149‑1 (MQ=40) gggggCACTGACCTGGAGGTCGGATAAGACGGATGGCGTCGGATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGAt < 2:66709/145‑1 (MQ=255) gTGCACTGAACTGTAGGGCGGCTAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGc < 1:459034/149‑1 (MQ=255) gCACTGACCTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCt < 1:974344/149‑1 (MQ=38) gAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATgc < 2:1714607/149‑1 (MQ=38) gcTGGAGGTCGGATAAGACGCATGGCGTCGTATTCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATgcgc < 2:1573239/148‑1 (MQ=255) gTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATACGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgccgc > 1:1669712/1‑149 (MQ=38) gTAGGTCGGATAAGACGGATGGCAACGGATGTGTCAACCGATGCCTGATGCGCCGCGGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAAACGCATAGCGTCGCATCCGACAACCGATGCCTGATGgcccgc > 2:1749941/1‑149 (MQ=11) ggTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGa < 1:459851/149‑1 (MQ=37) gTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGTACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAc < 2:755565/149‑1 (MQ=38) tCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCAATGCCTGATGCCCCGCGGAcg > 2:680347/1‑149 (MQ=255) gATACGACGCATGGGGTGGCATCCGACAACCGATGCCGGATGCGCCGCTGACGTGACATATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGTATAGCGTTGCATCCGACAACCGCTGCCTGATGCGCCGCTGACGCGa < 2:1355298/149‑1 (MQ=11) aTAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGAAGCCTGATGCGCCGCTGACGCGAc < 1:1001703/149‑1 (MQ=37) aTAAGACGGATGGCGTCGCATCCGACAACCGATGCATGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGAc < 1:1691477/149‑1 (MQ=37) aaGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACtt < 1:1204792/149‑1 (MQ=38) gACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTAt < 2:889483/149‑1 (MQ=37) gACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTAt < 1:1610724/149‑1 (MQ=37) gACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCCCCGCCGACACGACCTAt > 2:1437005/1‑149 (MQ=255) gACGGATGGCGCCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGGGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACACCCGCTGCCTGATGCGCCGCTGCCGCGACtcac > 2:249869/1‑146 (MQ=11) aCGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCCATGCCTGACGCGCCGCTGACACGACttcta > 2:1159436/1‑146 (MQ=255) aCGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCACAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATc > 1:415212/1‑149 (MQ=38) | TACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATC > NC_000913/349525‑349818 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |