Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,917,417 C→A 7.9% G283V (GGG→GTG)  atpG ← F1 sector of membrane‑bound ATP synthase, gamma subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,917,4170CA 7.9% 372.1 / 2.3 152G283V (GGG→GTG) atpGF1 sector of membrane‑bound ATP synthase, gamma subunit
Reads supporting (aligned to +/- strand):  ref base C (74/63);  new base A (2/13);  total (76/76)
Fisher's exact test for biased strand distribution p-value = 4.90e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

Only 100 of 159 total aligned reads displayed.

CCATTTTGCACCTCAAGAGCATCGTACACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTC  >  NC_000913/3917271‑3917565
                                                                                                                                                  |                                                                                                                                                    
ccATTTTGCACCTCAAGAGCATCGTACACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCGGTTTAAACCGCGGCGGccccc                                                                                                                                                    >  2:1441659/1‑149 (MQ=255)
 cATTTTGCACCTCAAGAGCATCGTACACGAGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGGTTACCTGGACAATCGGTCCGGTAGCCGTCATAAATCCGCTACGAAATAACCTGGTTAAACCGCGGCGTCCCCCg                                                                                                                                                   >  1:1274015/1‑149 (MQ=255)
  aTTTGGCACCTCAAGAGCAGCGTACACGCGCGGTACGGCATCCTGAGGGAATTCGCCGGCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCga                                                                                                                                                  <  1:630337/149‑1 (MQ=255)
   ttttGCACCTCAAGAGCATCGTACACGCGCGGTACGGCATCCTGAGGGGATTCGAGGTTTACGACGGGGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTGCGAAATAACCTGTTTAAAGCGCGGCGGCCCCCgag                                                                                                                                                 >  1:1414218/1‑149 (MQ=255)
   ttttGCACCTCAAGAGCATCGTACACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAGACCGCGGCGGCCCCCgag                                                                                                                                                 >  1:278928/1‑149 (MQ=255)
   ttttGCACCTCAAGAGCATCGTACACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCgag                                                                                                                                                 >  1:248160/1‑149 (MQ=255)
   ttttGCACCTCAAGAGCATCGTACACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCgag                                                                                                                                                 >  1:1103646/1‑149 (MQ=255)
           cTCAAGAGCATCGTGCACGCGCGGTACGGAATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGAtctc                                                                                                                                         <  1:317221/149‑1 (MQ=255)
            tCAAGAGCATCGTACACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGGGACGAtctcc                                                                                                                                        >  1:597806/1‑148 (MQ=255)
            tCAAGAGCATCGTACACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTAACTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACAAAATAACCTGTTTAAACCGCGGCGGCGCCCGACACGAtctgt                                                                                                                                        >  2:1636965/1‑147 (MQ=255)
              ctgggCATCTTCCCCGCCCGGTACTGCCTCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATTTTTTTTTTATGCTACGGCGACCACCGAGACGATCTCGGt                                                                                                                                      <  2:387821/145‑1 (MQ=255)
              aaGAGCATCGTACACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGt                                                                                                                                      <  1:249137/149‑1 (MQ=255)
               agggCATCGTACACGCGCGGTACGGCATCCTGGGGGAAGTCTACGTCGCCTACGGCTCTGTTTACCTGGATAATCGTTTCAGTAGCCATCTTAACTCCTCTACGAAATATCCTTTTAATGATACGGCGGCCACCGAGACGATCTCGGTg                                                                                                                                     <  2:1549139/146‑1 (MQ=255)
                gagCATCGTACACGCGCGGTACGGCATCCGGAGGGAATTCGTCGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATTTTTTTTTAATGATGCGGCGACCACCGAGACGATCTCGGTGa                                                                                                                                    <  2:262671/149‑1 (MQ=255)
                   cATCGTACACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCCGGACGAGCTCGGGCAGtt                                                                                                                                 >  2:1484115/1‑149 (MQ=255)
                    aTCGTACCCGCGCGGTACGGCACGCGGGGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGACATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTc                                                                                                                                <  1:299894/149‑1 (MQ=255)
                     tCGTACACGTGCGGGACGGCATCCTGAGGGAATTCGACCTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAATTTTTTTTTTAATGCTGCGGCGACCACCGAGACGATCTCGGTGAGTTcc                                                                                                                               <  2:1006583/149‑1 (MQ=255)
                        tACACGCGCTGTACGGCATCCTGAGTGAATGCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGATTTTTTTTTTTAATGACGCGGCGGCCACCGAGACGATCTCGGTGAGTTCCTGa                                                                                                                            <  2:1068387/149‑1 (MQ=255)
                         acacGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAg                                                                                                                           <  1:891655/149‑1 (MQ=255)
                           acGCGCCGTACCTGATCCCTAGGTAATTCGACGCCAACTACGGCGCCGATTACCTGGTCAATCTTTCCAGTAGCCATCTTAAATCCTCTACGATATTTTCTTTTTATGCTACGGCGACCACCGAGACGATCTCGGTGAGTTCCTGAGTa                                                                                                                         <  2:775025/149‑1 (MQ=255)
                            cgcgcgGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTCTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTaa                                                                                                                        <  1:98126/149‑1 (MQ=255)
                             gcgcgGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAAt                                                                                                                       >  1:485405/1‑149 (MQ=255)
                             gcgcgGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAAt                                                                                                                       <  1:125235/149‑1 (MQ=255)
                              cgcgGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCGTTCCAGTAGCCAGCTTAAATCCTCTACGAAATTTTTTTTTTATGATGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATg                                                                                                                      <  2:1136263/149‑1 (MQ=255)
                               ggtgTACGGCACCCTGCCGGCTTTTGACGTGACCTACGGTGCCGCTTACCCGGACAATCTTTCCACTAGCCCTCCTCCAACCCCCACCGCATGGCTTGTTGATGATACGGCGACCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGc                                                                                                                     <  2:831314/146‑1 (MQ=255)
                               gcgGGACGGCAGCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAACCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGc                                                                                                                     <  1:501464/149‑1 (MQ=255)
                                  gTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCGGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTAGGAAATAACCTGTTTAAACCGCGGCGGCCCGCGAGACGATCTCGGTGAGTTCGTGCGTAATGCTgg                                                                                                                  >  1:180379/1‑149 (MQ=255)
                                     tgtcATCCTGAGGGAAGTCGACGTCAATTTCTGCCCTGATGACCTGGACACTCTTTCCAGTAGCCATCTTAAACCCTCTACGAATTTTCCTGTTAATGCCGCGGCGACCACCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCt                                                                                                               <  2:1140477/146‑1 (MQ=255)
                                      ggCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCGCCCGTGGCGCTCTCGGTGAGTTCTTGGGTATTGCTGGCCTg                                                                                                              >  1:649212/1‑149 (MQ=255)
                                       gCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGa                                                                                                             >  1:1149161/1‑149 (MQ=255)
                                       gCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCCGGCCTGa                                                                                                             >  2:269417/1‑149 (MQ=255)
                                            cTGAGGGAGTTCGTCGTCAACTGCGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGTAATTTCTTTTTAATGCTGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGc                                                                                                        <  2:1571105/149‑1 (MQ=255)
                                            cTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTAAGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGc                                                                                                        >  1:1808100/1‑149 (MQ=255)
                                             tGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCt                                                                                                       >  2:975236/1‑149 (MQ=255)
                                             tGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCt                                                                                                       >  2:1546021/1‑149 (MQ=255)
                                             tGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCt                                                                                                       >  1:1633003/1‑149 (MQ=255)
                                             tGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGAAGCTATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCt                                                                                                       >  1:1706986/1‑149 (MQ=255)
                                              gAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGGGTTCCTGAGTAATGCTGGCCAGACGAGCtt                                                                                                      >  2:557204/1‑149 (MQ=255)
                                               agtcatgtCGACGTGAACGACGGCGCCGATTACCTTGACAATCTTTCCAGTAGCCATCTTAATTCCTCTACGAATTTTTTTTTTAATGATGCGGCGACCACCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCttt                                                                                                     <  2:1174782/142‑1 (MQ=255)
                                               aGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCttt                                                                                                     <  1:798823/149‑1 (MQ=255)
                                               aGGGAATTCGACGCCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCttt                                                                                                     <  1:811184/149‑1 (MQ=255)
                                                 ggAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTttgt                                                                                                   >  1:634767/1‑149 (MQ=255)
                                                  gAATTCGCCGTCGACTACGGCTCTGATTACCTTGACTATCTTTCCATTAGCCATCTTAAATCCTCTACGTATTTTCTTGTTAATGACACGGCGACCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTttgtt                                                                                                  <  2:47356/149‑1 (MQ=255)
                                                  gAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTttgtt                                                                                                  <  1:1425407/149‑1 (MQ=255)
                                                  caaTTCGACGTCAACCACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATACCCTGTTTAAACCGCCGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTttgtt                                                                                                  <  1:1202408/148‑1 (MQ=255)
                                                   taTTCGACGTCAACTACGGCGCCGATTACCTGGACATTGTTTCCAGGAGCCATCTTAAATCCTCTACGAGTTTTCTTGTTAATGCCGCGGCGGCCACCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTttgttg                                                                                                 <  2:86979/148‑1 (MQ=255)
                                                     ttCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGta                                                                                               >  1:480920/1‑149 (MQ=255)
                                                     ttCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGta                                                                                               >  1:1172814/1‑149 (MQ=255)
                                                      gcGACGTCAACTACGCCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCGTCTACGAATTTTTCTTTTAATGCTGCGGCGGCCACCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGtat                                                                                              <  2:286863/148‑1 (MQ=255)
                                                        gACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATAc                                                                                            <  1:201125/149‑1 (MQ=255)
                                                            tCAACTACGGCGCCGATTACCTGGACAATCTTTGCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGGGTGCCTGATTGTTGCTGGCCTGACTGGCTTTGTTGtagccctac                                                                                        >  2:1751665/1‑142 (MQ=255)
                                                            tCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCATCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTGCTGAGTAATGCTGGCCCGAAGGGCTTTGTTGTATACCCAc                                                                                        >  1:1364296/1‑149 (MQ=255)
                                                              aaCTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGACCTGACGAGCTTTGTTATCTACCAACta                                                                                      >  2:652988/1‑148 (MQ=255)
                                                              aaCTACGGCGCCGATTACCTGGACAATCATTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCAGGCCCGACGAGCTTTGTTGTATACCAAccg                                                                                      >  2:717624/1‑147 (MQ=255)
                                                                   cGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCGTAAATCCTCTACGAGATAACCTGTTTAAGCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCATGAGTAATCCTGGCCTGACGAACTTTGTTGTATACCAAccaaaaaa                                                                                 >  2:182546/1‑142 (MQ=255)
                                                                     gcgcCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGctct                                                                               <  1:1603591/149‑1 (MQ=255)
                                                                      cgcCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCtt                                                                              <  1:555355/149‑1 (MQ=255)
                                                                        ccGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATTTCCTGTTTATGCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTa                                                                            <  2:1699022/149‑1 (MQ=255)
                                                                        ccGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTCATGCTGGCCTGACGGGCTTTGTTGTATACCACCTGCGCATCTTTa                                                                            >  2:844234/1‑149 (MQ=255)
                                                                            ttACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCa                                                                        <  1:1278109/149‑1 (MQ=255)
                                                                            ttACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTGGTATACCAACTGCAGCTCTTTAATCa                                                                        >  1:739092/1‑149 (MQ=255)
                                                                                   gACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGGGCTCCTGCGTAATGCTTGCCTAACGAGCTTTCTTGTATACCGACTGGCGCTCTTTACTCCGGCTgcc                                                                 >  2:602335/1‑149 (MQ=255)
                                                                                   gACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTgcc                                                                 >  1:837193/1‑149 (MQ=255)
                                                                                        tCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCGGCTCTTTAATCAGACTgccgcact                                                            >  2:1481328/1‑146 (MQ=255)
                                                                                        tCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCAt                                                            >  1:158690/1‑149 (MQ=255)
                                                                                          tttCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTg                                                          >  1:747852/1‑149 (MQ=255)
                                                                                           tcccACTAGCCATCTTAAATCCTCTACAAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTgt                                                         <  1:921188/147‑1 (MQ=255)
                                                                                             ccAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCGTAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTgtcg                                                       <  1:404473/149‑1 (MQ=255)
                                                                                              cAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTgtcgg                                                      >  1:1208755/1‑149 (MQ=255)
                                                                                               aGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTgtcggt                                                     >  1:1762055/1‑149 (MQ=255)
                                                                                                gTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCCGCTCTTTAATGAGGCTNCGGCCACTgtcggcc                                                    >  1:1663659/1‑147 (MQ=255)
                                                                                                     cATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGc                                               >  1:1452180/1‑149 (MQ=255)
                                                                                                       tCTTAAACCCTCGACGAAATAACCTTGTTAAACCGGGGCGTCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCCCGGCtt                                             <  1:889527/149‑1 (MQ=255)
                                                                                                        cTTAAATCCTCTACGTAATAAGCTGTTAAAGCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCttt                                            <  2:1172814/149‑1 (MQ=255)
                                                                                                         ttAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTACTGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTACTCAGGCTGACGCCATTGTCGGTCGCGGCTTTc                                           >  2:681731/1‑149 (MQ=255)
                                                                                                         ttAAATCCTCTACGAAATAACCTGTTGAAACCGCGGCGGCCACCGAGACGATCTCGGTGAGTGCCGGAGTAATGCGGGCCTGAGGAGACTTGTTGTATACCAACTGAAGCTCCTTAATCGGACTGCCGTCATCGTCGGTCGCGGCTTTc                                           >  2:1467915/1‑149 (MQ=255)
                                                                                                         ttAAATCCTCTACGAAATAACCTGATTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAAGTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTTGCGGCTTTc                                           >  2:914795/1‑149 (MQ=255)
                                                                                                           aaaTCCTCTACGAATTTACTTTTTTAAGCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCAt                                         <  2:344046/149‑1 (MQ=255)
                                                                                                            aaTCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTCGCCTGACGAGCTTTGTGGTATCCCAACTGCCGCTCTTTAATCAGGCTCCCCCCATTGTCGGTCGCGGCCTCCATc                                        >  2:714986/1‑149 (MQ=255)
                                                                                                             aTCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTAGGTGAGTTCCTGAGTAATGCTGACCTGACGAGCTTTGTTGTATACCAACAGCAGCGCTTTAATCAGGCTGCCGCCGTTGTCGGTGGCGGCTTTCATCg                                       >  2:596298/1‑149 (MQ=255)
                                                                                                              tCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGc                                      <  1:1559388/149‑1 (MQ=255)
                                                                                                                 tctACGCTATGACCTGTTCAACCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGccac                                   <  1:1443971/149‑1 (MQ=255)
                                                                                                                 tctACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGccac                                   >  1:925349/1‑149 (MQ=255)
                                                                                                                  ctccGAAATTCCCTTTTAAAGCTGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGccacc                                  <  2:66891/146‑1 (MQ=255)
                                                                                                                  ctACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGCTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTCTACCAACTGCAGCTCTTTAATCGGGCTGCCGCCATTGTCGGTGGCGGCTTTCATCcccccc                                  >  2:1577727/1‑149 (MQ=255)
                                                                                                                    ccGAAATACCCTGTTTACACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCAt                                <  1:546697/148‑1 (MQ=255)
                                                                                                                       aaaTAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACg                             <  1:1744495/149‑1 (MQ=255)
                                                                                                                             ccTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGTCTGCCGCCATTGTAGGTCGCGGCTTTCATCGCCACCATACGggcggc                       <  1:1165193/149‑1 (MQ=255)
                                                                                                                              cTGTTTAAACCGCGGCGGCCACCGAGACGATCACGGTGAGGTCCTGAGTAATGCTGACCAGACGAGCTTTGGTGAATAGCGACTACAGCTCTTTAAGCAGGCTGCCGCCATGGTCGGTCCGGACCTTAATAGCCACCATACGAGCGGcc                      >  2:879353/1‑149 (MQ=255)
                                                                                                                                gtgtAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTg                    <  1:669511/146‑1 (MQ=255)
                                                                                                                                 tttAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGc                   >  1:279421/1‑149 (MQ=255)
                                                                                                                                   tacaCCGCGGCGACCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTc                 <  2:1265120/146‑1 (MQ=255)
                                                                                                                                   tAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTc                 <  2:934084/149‑1 (MQ=255)
                                                                                                                                      aCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCt              <  2:1514497/149‑1 (MQ=255)
                                                                                                                                      aCCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTATTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCt              <  1:1227193/149‑1 (MQ=255)
                                                                                                                                       ccGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTg             >  1:28259/1‑149 (MQ=255)
                                                                                                                                        cgcgGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTgg            >  2:1719229/1‑149 (MQ=255)
                                                                                                                                              ggcCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGt      <  2:1633003/149‑1 (MQ=255)
                                                                                                                                                  cccGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGGATAACAACTGCAGCTCTTTAATCAGGCTGCCGCCGTTGTCGGTCGCGGCTTTCATCGCCCCCCTACGGACGGCCTGCTCGCTGGCCAGGGTTTc  >  1:389140/1‑149 (MQ=255)
                                                                                                                                                  cccGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAACTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGACTGCTCGCTGTCCAGGTTTTc  >  1:1257584/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CCATTTTGCACCTCAAGAGCATCGTACACGCGCGGTACGGCATCCTGAGGGAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTTC  >  NC_000913/3917271‑3917565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑