Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,935,065 C→A 5.6% A430E (GCG→GAG)  rbsA → D‑ribose ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,935,0650CA 5.6% 439.1 / 3.0 155A430E (GCG→GAG) rbsAD‑ribose ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (74/67);  new base A (2/8);  total (79/76)
Fisher's exact test for biased strand distribution p-value = 5.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 158 total aligned reads displayed.

CTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTAC  >  NC_000913/3934917‑3935209
                                                                                                                                                    |                                                                                                                                                 
cTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGgcgc                                                                                                                                                   >  1:956768/1‑149 (MQ=255)
   tttAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTATGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCGGATGAAAAGCCCCAAAGCGTTGATCCTTGATGAACCTGCCCGTGGCGTAGAAGTCGGcgcgga                                                                                                                                                >  2:359887/1‑147 (MQ=255)
   attAATGTGAAAACTCCGTCGATGGAACAGGCAGTTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGAGGCGATTGCCCGCGGACTGCTGACACGCCCCACAGTGGTGATCCTTGATGCAACTACCCGTGGCGTAGATGTCAGCCCGaa                                                                                                                                                >  2:154224/2‑149 (MQ=255)
    ttAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGaaa                                                                                                                                               >  1:1225398/1‑149 (MQ=255)
    ttAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGaaa                                                                                                                                               >  2:806907/1‑149 (MQ=255)
     tAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGaaaa                                                                                                                                              >  2:1315205/1‑149 (MQ=255)
      aaTGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGAGGCGTAGATGTCGGCGGGaaaaa                                                                                                                                             >  2:1759343/1‑149 (MQ=255)
       aTGTGAAAACTTCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTAGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCTCCAAAGCGTTGATCCTTGATGAACCTACCCGTGTCGTAGATGTCGGCGCGaaaaaa                                                                                                                                            <  2:1177711/149‑1 (MQ=255)
         gtgAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCCATGTGTTGATCCTTGATGAACCTACCCGTGGCGTGGATGTCGGCTCGAAAAAaga                                                                                                                                          >  1:324670/1‑149 (MQ=255)
          tgAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAagag                                                                                                                                         >  1:1040008/1‑149 (MQ=255)
          tgAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATGGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAagag                                                                                                                                         >  2:773257/1‑149 (MQ=255)
           gAAAACCCCGGCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGAGGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTGGGCGCGAAAAAAgaga                                                                                                                                        <  1:1144938/149‑1 (MQ=255)
                    gTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATTTCGGCGCGAAACAGGAGCTCTATCAAc                                                                                                                               >  1:524682/1‑149 (MQ=255)
                    gTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCACGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTAtatac                                                                                                                               >  1:1219326/1‑145 (MQ=255)
                      cGATGGAGCAGGCGATTGGTCTGCTTTCCGGTGGCAATCTGCAAAAAGTGGCGATTGGCCGCGGTCTGATGACACGCCCCAAAGTGTTGTTCCTTGATGATCCTACTCTTGGCGTTGATGTAAGAGAAAAAAAAGAGATCTATCAACTg                                                                                                                             <  2:620038/149‑1 (MQ=255)
                       gATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGa                                                                                                                            <  1:1566439/149‑1 (MQ=255)
                            aaCAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAAc                                                                                                                       >  2:295880/1‑149 (MQ=255)
                            aaCAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAAc                                                                                                                       <  1:357800/149‑1 (MQ=255)
                               aGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAg                                                                                                                    >  2:1044449/1‑149 (MQ=255)
                                 gCAATTGGTCTGCTTTCCTGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGtt                                                                                                                  <  1:1617743/149‑1 (MQ=255)
                                 gCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGtt                                                                                                                  <  2:226131/149‑1 (MQ=255)
                                  cAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTc                                                                                                                 <  1:1808838/149‑1 (MQ=255)
                                   aaTTGGTCTGCATTCCGGTGGCAATCAGCAAAAAGTGACGGTTGCCAGCGGTCTGATGACACGCCCCAAAGTGTCGATCCTTGATGAACTTACCCGTGGCGTAGAAAAAAAAAAAAAAAAAGAGATCTATCAACTGATTAACCAGTTCa                                                                                                                <  2:242178/149‑1 (MQ=255)
                                    agtGGGCTGCTTTGCGCTAGCAGTCGGCACAATGTGGGCGTTCTCCGCGGTCTGATGACACGCCCTAACGTGTTGGTCCTTGATGAACCGACCCGTGGCGTAGATGTCGGCTCAAAAAAAGAGATCTATCAACTGATTAACCAGTTCaa                                                                                                               <  2:443353/147‑1 (MQ=255)
                                    aTTCGTCTGTCTTCCGGTGGCAATCGGCAAAAAGTGGCGATTGCCCGCGGTCTGACGACACGCCCCAAAGTGTTTATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCaa                                                                                                               <  2:1804178/149‑1 (MQ=255)
                                     ttGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCaaa                                                                                                              <  1:1084974/149‑1 (MQ=255)
                                        gTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGACCCTACCCGTGGCGTAGATGTCGGCGGGAAAAAAGGGATCTATCAACTAATTAACCAGTTCAAAGcc                                                                                                           >  2:1250685/1‑149 (MQ=255)
                                        gTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGGAAAAAGAGATCTATCAACTGATTGACCAGTTCAAAGcc                                                                                                           >  1:28072/1‑149 (MQ=255)
                                          cTGCTTTCCGGTGGCAATCGGCAAAAAGTGGTGACTGCGCGCGGCCCGCTGACACGCCCCAAAGTGTTGATACTTGATGAACTGCCCCGTGGCGTAGAAAAAAAAAAAAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGa                                                                                                         <  2:804566/149‑1 (MQ=255)
                                           tGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGAt                                                                                                        >  2:847397/1‑149 (MQ=255)
                                           tGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGAt                                                                                                        >  1:286906/1‑149 (MQ=255)
                                             cTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATgg                                                                                                      >  1:504094/1‑149 (MQ=255)
                                                 cctggggCAACCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTg                                                                                                 <  1:1527725/144‑1 (MQ=255)
                                                 ccGGTTGTAATCAGCAAAAAGTGGCGATTAGCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTg                                                                                                 <  2:36638/149‑1 (MQ=255)
                                                    gTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGc                                                                                              <  2:417941/149‑1 (MQ=255)
                                                         aaTCAGCAAAAAGTGGCGCTTGCCCGCCGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGCGGCGTCGATGTCGGCGCGCGACAAGAGATCTATCAACTGAGTAACCAGTTCGAAGCCGATGCATTGAGcatcat                                                                                         >  2:167508/1‑149 (MQ=255)
                                                         aaTCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGcatcat                                                                                         >  1:642738/1‑149 (MQ=255)
                                                           tCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTc                                                                                       >  1:1435093/1‑149 (MQ=255)
                                                           tCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTc                                                                                       >  2:67546/1‑149 (MQ=255)
                                                             aGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTg                                                                                     <  1:1147268/149‑1 (MQ=255)
                                                             aGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTg                                                                                     <  1:425903/149‑1 (MQ=255)
                                                             aGCAAAAAGTGGAGATTGCCCGCGGTCGGATGACACGCCCCAAAGTGGTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAGAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCTTCATTCTg                                                                                     >  2:1517624/1‑149 (MQ=255)
                                                              gCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGACGTAACGACCCATGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTgg                                                                                    >  1:708523/1‑149 (MQ=255)
                                                               cAAAAAGTGGCGATTGCCCGCGGCCTGATGACGCGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGgt                                                                                   <  2:568846/149‑1 (MQ=255)
                                                                 aaaaGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGgtgt                                                                                 >  1:1518967/1‑149 (MQ=255)
                                                                    aGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCAt                                                                              <  2:1555236/149‑1 (MQ=255)
                                                                     ttGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGCGTTGATCCTTGATGAACCCACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATc                                                                             <  1:1357764/148‑1 (MQ=255)
                                                                     ggggCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATc                                                                             <  1:196513/147‑1 (MQ=255)
                                                                     gTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATc                                                                             >  1:822007/1‑149 (MQ=255)
                                                                     gTGACGAGTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGGGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGAGTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATc                                                                             <  1:62960/149‑1 (MQ=255)
                                                                      tGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGACCATCATTCTGGTGTCATcc                                                                            >  2:1462271/1‑148 (MQ=255)
                                                                          gATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGgaga                                                                        >  1:1091928/1‑149 (MQ=255)
                                                                          gATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGgaga                                                                        >  1:1037818/1‑149 (MQ=255)
                                                                           aTTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGAGTCATCGGAGAt                                                                       >  2:78276/1‑149 (MQ=255)
                                                                             tGCCCGCGGTGTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGc                                                                     <  1:1634956/149‑1 (MQ=255)
                                                                             tGCCCGCGGTCTGATGACACGCCCCAATGTGTTGACCCTTGATGAACCCACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGc                                                                     <  2:1545621/149‑1 (MQ=255)
                                                                               cccGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTATCCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCa                                                                   >  2:1001635/1‑149 (MQ=255)
                                                                               cccGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCa                                                                   <  2:1040008/149‑1 (MQ=255)
                                                                               cccGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGGGTCATCGGAGATGCCa                                                                   >  2:525112/1‑149 (MQ=255)
                                                                                ccGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAg                                                                  >  1:1253135/1‑149 (MQ=255)
                                                                                    gttCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGGGTCATCGCAGATGCCCGAAGt                                                              >  2:1671164/3‑149 (MQ=255)
                                                                                    ggTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAGAAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTTTGGTGTCATCGCAGATGCCAGAAGt                                                              >  2:1144532/1‑149 (MQ=255)
                                                                                     gTCTGATGACACACCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTa                                                             >  1:92163/1‑149 (MQ=255)
                                                                                      tCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTAt                                                            >  2:1111272/1‑149 (MQ=255)
                                                                                        tgatgaCACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTGACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTa                                                          >  2:1386419/1‑149 (MQ=255)
                                                                                        tgatgaCACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTa                                                          <  1:1354196/149‑1 (MQ=255)
                                                                                        tgatgaCACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTa                                                          <  2:1122610/149‑1 (MQ=255)
                                                                                         ggtgaCACGCCCCAACGTAGTGCTCCTGGAGGAACGTACCCTTGGCGTAGAAGTCGGCACAAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAg                                                         <  2:1220332/147‑1 (MQ=255)
                                                                                         ggtgaCACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTAGCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAg                                                         <  2:870336/147‑1 (MQ=255)
                                                                                          atgaCACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCGTCATTCTGGGGTCATCGGGGAGGACAGGAGTATTAgt                                                        >  1:1017440/1‑148 (MQ=255)
                                                                                            gccacGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCa                                                      <  1:1691348/147‑1 (MQ=255)
                                                                                               acGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGACAGAAGTATTAGGCATgg                                                   >  1:130454/1‑148 (MQ=255)
                                                                                                     cAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGCGCGATc                                             >  1:175320/1‑149 (MQ=255)
                                                                                                      aaaGTGTGGAGCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCg                                            <  2:869510/149‑1 (MQ=255)
                                                                                                         gtgtTGATCCTTGATGGACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTTATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGcac                                         >  2:1425841/1‑148 (MQ=255)
                                                                                                          tgtTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGcatc                                        >  1:832486/1‑149 (MQ=255)
                                                                                                           gtTGATCCTTGATGAACCTACCCGTGGCGGAGATGTCGGCGCGAAAAAAGAGACCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGCGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGTGCGATCGcatca                                       <  1:1390969/149‑1 (MQ=255)
                                                                                                            ttGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGcatcat                                      >  1:1511088/1‑149 (MQ=255)
                                                                                                             tGACCCGTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCatcatc                                     <  2:413099/149‑1 (MQ=255)
                                                                                                              gATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGACGTATTCGGCATGAGCGATCGCATCATCg                                    >  1:1131698/1‑149 (MQ=255)
                                                                                                              gATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATCGCTCTGACATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCg                                    >  1:1780051/1‑149 (MQ=255)
                                                                                                               aTCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCTCGCCATAACATCAATCAACAAATTAACCAGTTCAAAGCCGATGGCTTGAGAATCATTCTGGTGTCATCGTAGCTGCCAGAAGTACTAGGCATGAACGCTAGCATCATCGt                                   >  2:261398/1‑149 (MQ=255)
                                                                                                                tCCTTGATGAACCTACCCGTGGTGTAGATGTCGGTGGTCGCAGTGAGATTTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCTTCGTc                                  >  2:1767444/1‑149 (MQ=255)
                                                                                                                  cTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGCAGTATTAGGCATGAGCGATCGCATCATCGTcat                                >  2:1359347/1‑149 (MQ=255)
                                                                                                                  cTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCCTCGTcat                                >  1:448735/1‑149 (MQ=255)
                                                                                                                    tgatgaACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTTGGCATGAGCGATCGCATCATCGTcatgc                              <  1:1183177/149‑1 (MQ=255)
                                                                                                                     gatgaACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCCTTCTGGTGTCATCGGAGATGCCAGAAGGATTAGGCATGAGCGATCGCATCATCGTcatgca                             >  2:441346/1‑149 (MQ=255)
                                                                                                                     gatgaACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTcatgca                             <  1:994255/149‑1 (MQ=255)
                                                                                                                           ccTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAgg                       <  2:1635284/149‑1 (MQ=255)
                                                                                                                           ccTACCCGTGGCGTAGATGTCGGCGAGAGAAAAGAGATCTATCAACTCATTAACCAGTTCAAAGCCGATGTCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCAGCATCGTCATGCATGAAgg                       >  1:1013096/1‑149 (MQ=255)
                                                                                                                             tACCCGTGGCTTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTACCCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGc                     <  2:983256/149‑1 (MQ=255)
                                                                                                                                 cGTGGCGTAGATGTCGGCGCGAAAAAAGAGCTCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAGGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCAtct                 >  1:1337743/1‑149 (MQ=255)
                                                                                                                                 cGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCAGCGTCATGCATGAAGGGCAtct                 >  2:850997/1‑149 (MQ=255)
                                                                                                                                      cggaGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCg            <  1:404180/146‑1 (MQ=255)
                                                                                                                                      cGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCg            <  1:124217/149‑1 (MQ=255)
                                                                                                                                          gATGTCGGCCCGAAAAAAGCGCGCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGa        <  1:1440761/149‑1 (MQ=255)
                                                                                                                                           aTGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTGGAGCATGAGTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCCTGAAGGGCATCCCAGCggggga       >  2:831841/1‑147 (MQ=255)
                                                                                                                                              tCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATAATCGTCATGCATGAAGGGCATCTAAACGGGGAGttt    >  2:1152634/1‑149 (MQ=255)
                                                                                                                                               cGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTa   <  1:1758581/149‑1 (MQ=255)
                                                                                                                                                ggCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTAc  >  1:898182/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CTGTTTAATGTGAAAACTCCGTCGATGGAACAGGCAATTGGTCTGCTTTCCGGTGGCAATCAGCAAAAAGTGGCGATTGCCCGCGGTCTGATGACACGCCCCAAAGTGTTGATCCTTGATGAACCTACCCGTGGCGTAGATGTCGGCGCGAAAAAAGAGATCTATCAACTGATTAACCAGTTCAAAGCCGATGGCTTGAGCATCATTCTGGTGTCATCGGAGATGCCAGAAGTATTAGGCATGAGCGATCGCATCATCGTCATGCATGAAGGGCATCTCAGCGGGGAATTTAC  >  NC_000913/3934917‑3935209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: