Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 375,176 A→G 6.1% intergenic (+295/‑283) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913375,1760AG 6.1% 418.1 / 2.2 174intergenic (+295/‑283)mhpE/mhpT4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base A (75/80);  new base G (15/3);  total (91/83)
Fisher's exact test for biased strand distribution p-value = 5.54e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

ACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTG  >  NC_000913/375028‑375324
                                                                                                                                                    |                                                                                                                                                    
aCTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGAGAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCga                                                                                                                                                      <  1:1369433/149‑1 (MQ=255)
   gCCGGATGCGGCGTGAACGCCTTAACCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGGCGCGTGAGCGGCGCGTCAGGCATCTGCGGACGAATGCCGGATGCGGGGTGAACGCCTTATCCGGCCTACGGGTGGCGCgagac                                                                                                                                                   <  2:1424700/149‑2 (MQ=14)
     cGGATGCGGCGTGAACGCCGGATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAAtt                                                                                                                                                 >  2:1796048/1‑149 (MQ=255)
      ggATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAGTACTCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGAGAACGCCTTATACGCCCTACGGCTGGCGCGAGAAttc                                                                                                                                                >  1:1413191/1‑148 (MQ=14)
        agggggcgTGGACGCCTTATCCGGCCGACGGATGGCGCGGGAATTTGGAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGt                                                                                                                                              <  2:349653/145‑1 (MQ=255)
         ggcggcgGGAAGGCCTTCTCCGGCCGACGGATGGGGCGGGAAGTTGTAGGCCGGATAAGACGCGTTAGCGCCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTa                                                                                                                                             <  2:1615704/148‑1 (MQ=14)
          gcggcgTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGAATGCCGGATGCGGCGTGAACGCCCTATCCGGCCCACGGGGGGCGCGAGGACTTggca                                                                                                                                            >  2:475467/1‑146 (MQ=14)
           cggcgTGATCGCCTTATCCGGCCTACGGAGGGCGCGGCAATTGGTAGGGCTGCTAAGACGCGTTAGCGTCGCCTCAGGGATCTACTTACGGCTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCTAGAATTTGTAgg                                                                                                                                           <  2:751352/149‑1 (MQ=14)
            ggcgTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGCACGCCTTCTCCGCCCCACGCGTGGCGCGGGAATTCGTAAGc                                                                                                                                          >  2:424820/1‑149 (MQ=11)
            ggcgTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGAGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGc                                                                                                                                          >  2:1165135/1‑149 (MQ=255)
              cgTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCt                                                                                                                                        <  2:902970/149‑1 (MQ=255)
              cgTGAACGCCTTATCCGGCCGACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGATAATTTGTAGGCCt                                                                                                                                        <  1:670743/149‑1 (MQ=255)
                      ccTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTCTCCGGCCCACGGGTCGCGCGGGAAGTTGTAGGCCCGATAAgcc                                                                                                                                >  2:248877/1‑147 (MQ=11)
                        tttgccGGCCGAGGGATGGCGTGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCGGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGAcgc                                                                                                                              <  2:430561/145‑1 (MQ=11)
                        ttATCCGGCCTACGGATGGCGCGCGAATTTGTAGGCCGGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGAcgc                                                                                                                              <  2:493692/149‑1 (MQ=255)
                           tCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGtt                                                                                                                           <  1:743935/149‑1 (MQ=255)
                           tCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGATAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGAAGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAAGTTGTATGCCTGATACGAcgcggt                                                                                                                           >  2:1354350/1‑147 (MQ=11)
                                 cTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTc                                                                                                                     >  1:314131/1‑149 (MQ=255)
                                 cTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGAGAGCGCCGTACCCGGCCCACGTGGGCCGCGGGCAGTTGTAGGCCTGATAGGACGCGTTAGCggc                                                                                                                     >  2:1005719/1‑147 (MQ=11)
                                  tACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGCTGCGGCGTGAGCGCCTTATCCGGCCTCCGGGTGGCGGGGGCATTTGTCGGCCTGATAAGAGGCGTGAggagcg                                                                                                                    >  1:458004/1‑144 (MQ=11)
                                   aCGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGc                                                                                                                   <  1:241166/149‑1 (MQ=255)
                                    cGGATGGCGCGGGAGTTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGCCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCa                                                                                                                  <  2:1039130/149‑1 (MQ=255)
                                    cGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCa                                                                                                                  <  1:483969/149‑1 (MQ=255)
                                       agggCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCa                                                                                                               <  2:1487751/147‑1 (MQ=255)
                                       aTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCCACGGGTGGCGCGGGAATTTGTAGGCCTGATTAGAGGCGCTAGCGCCGCAACa                                                                                                               >  2:91710/1‑149 (MQ=11)
                                           cgcgGGAATTTGTAGGCCTGATAAGCCGCGTTAGCGTCCCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGTCCTGATAAGACGCGTTAGCGTCGCATCAGGCa                                                                                                           <  2:391407/149‑1 (MQ=255)
                                            gcgGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCAGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCGTGATAAGACGCGTTAGCGTCGCATCCGGCAt                                                                                                          >  1:1216431/1‑149 (MQ=255)
                                                  aTTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTgcgc                                                                                                    >  1:788566/1‑149 (MQ=38)
                                                      gTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGa                                                                                                <  1:1076755/149‑1 (MQ=39)
                                                          gCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGAGGTGACGGATATTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGc                                                                                            >  1:1321340/1‑149 (MQ=11)
                                                           cctggcaaGCCGCGTTAGCGTCGCGTCGGGCATCTGGGCACGACTGCCGGATGCTGCGTCGACGTCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGcc                                                                                           <  2:816166/143‑1 (MQ=11)
                                                           ccTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGcc                                                                                           <  2:1575026/149‑1 (MQ=35)
                                                             tGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGc                                                                                                                                          <  1:1146819/100‑1 (MQ=39)
                                                              gATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGa                                                                                        <  2:217579/149‑1 (MQ=34)
                                                              gATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGa                                                                                        <  2:1388189/149‑1 (MQ=35)
                                                                    aCGCGTGCGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATgcggc                                                                                  <  2:1073549/149‑1 (MQ=25)
                                                                         ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTaaa                                                                             >  1:381667/1‑149 (MQ=38)
                                                                         ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTaaa                                                                             <  1:1428098/149‑1 (MQ=37)
                                                                         ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGGaaa                                                                             >  2:600321/1‑149 (MQ=25)
                                                                          tAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAAc                                                                            <  2:970671/149‑1 (MQ=37)
                                                                              gTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCt                                                                        >  1:1479856/1‑149 (MQ=38)
                                                                                 gCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGCCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGCGAACGCCGTac                                                                     >  1:982998/1‑148 (MQ=255)
                                                                                  caacAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTAATc                                                                    >  1:1302771/4‑149 (MQ=12)
                                                                                  cATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGAAGAACTGATAAGAAGAGACAGCGCCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGGAAACGCCTTATc                                                                    >  1:791884/1‑149 (MQ=11)
                                                                                   aTCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATcc                                                                   >  1:642621/1‑149 (MQ=38)
                                                                                    tCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCt                                                                                                        >  2:1345390/1‑111 (MQ=35)
                                                                                      aGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCCGGCATCTGCGCACGACTGCCGTATGCGGCGTAAACGCCTTATCCGGc                                                                <  1:1013121/149‑1 (MQ=255)
                                                                                      aGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                <  1:1525309/149‑1 (MQ=38)
                                                                                      aGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                <  1:1545042/149‑1 (MQ=38)
                                                                                      aGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                <  2:1148440/149‑1 (MQ=38)
                                                                                        gCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTACACGCCTTATCCGGCCt                                                              >  2:322631/1‑149 (MQ=38)
                                                                                        gCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                              >  1:92753/1‑149 (MQ=38)
                                                                                        gCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGGAAGCGCCTTATCCCGCCt                                                              >  2:1519372/1‑149 (MQ=255)
                                                                                           tCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACg                                                           >  2:1294288/1‑149 (MQ=38)
                                                                                             tcagcACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGa                                                         <  1:1337947/146‑1 (MQ=38)
                                                                                             tGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGa                                                         <  2:1230380/149‑1 (MQ=38)
                                                                                             tGCGCACGACTGCCGGATGCGGCGTGAACGCCTCATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCATTATCCGGCCTACGGa                                                         >  2:1344346/1‑149 (MQ=12)
                                                                                             tGCGCACGACTCCCGGATGCGGCATGAACCGCTTATCCGGCCTACGGCTGGCGCGAGAATTTGTAAGCCTGATAAGACGCGTTGGCTTCCCATCAGGCAGCTGCGCACGACTGCCGGATGCGGCGAAAACCCCATGTCCTGCCTACGGa                                                         >  2:458766/1‑149 (MQ=11)
                                                                                                gcACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATgg                                                      >  1:540690/1‑149 (MQ=40)
                                                                                                 cACGACTGCCGAGTGCGGCGTGAACGCCGTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGCCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGgc                                                     <  2:638725/149‑1 (MQ=255)
                                                                                                       tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATGCGGCCTACGGCTGGCGCGGGa                                               >  2:1530890/1‑149 (MQ=255)
                                                                                                         ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATCAGACGCGTTAGCGTCGCATCAGGCATCTGCGCCCGACTGCCTGATGCGGCCGAAACGCCTTATCCGGCCTACGGATGGCGCgggcag                                             >  2:191357/1‑146 (MQ=14)
                                                                                                         ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATACGACGCGTTAGCGTCGCATCAGGCATCTGCGCCCGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGCCGCGGGAAt                                             >  1:1195515/1‑149 (MQ=255)
                                                                                                              tGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTa                                        <  2:568516/149‑1 (MQ=39)
                                                                                                                 ggcgTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCATTGGCCTGCCTACGGATGGCGCGGGAATTTGTAGGc                                     <  2:1748080/149‑1 (MQ=11)
                                                                                                                   cgcgAACTCCTTATCCGGCCTACGGGTGGCGGGAGAATGTGTAGGCCTGATAAGACGCGTGAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCt                                   <  2:653943/146‑1 (MQ=11)
                                                                                                                    gTGGACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTATGCCTGATAAGACGCGTTAGCGTTGCATCAGGCATCCGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTg                                  <  2:606776/149‑1 (MQ=11)
                                                                                                                     tGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGa                                 >  1:1042724/1‑149 (MQ=255)
                                                                                                                      gAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTGGCGCGGGAATTTGTAGGCCTGAt                                >  2:1759928/1‑149 (MQ=255)
                                                                                                                      gAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGAt                                <  2:1803455/149‑1 (MQ=39)
                                                                                                                       aaCGCCTTATCCGGCCTACGGGTGGCGCGAGCATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCAACAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATa                               >  1:722394/1‑149 (MQ=255)
                                                                                                                         cGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGATGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAg                             >  1:1296463/1‑149 (MQ=255)
                                                                                                                          gCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGa                            >  1:1654391/1‑149 (MQ=255)
                                                                                                                            cTTATCCGGCCTACGGGTGGCGCGCGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGAcg                          >  1:912848/1‑149 (MQ=255)
                                                                                                                            cTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTgcgc                                                                                                    <  1:234371/75‑1 (MQ=255)
                                                                                                                            cTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTGGCGCGGGCATTTGTAGGCCTGATAAGAcg                          >  1:194331/1‑149 (MQ=255)
                                                                                                                            cTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGAcg                          >  1:870245/1‑149 (MQ=255)
                                                                                                                             ttATCCGGCTTACGGTGGGCGCGATAATTCGTAGGACTGATAAGAGGCGTTAGCGTCGCATCAGGCAGCTGCGCACGACTGCCGAATGTGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGAcgc                         <  1:1255407/149‑1 (MQ=11)
                                                                                                                             ttATCCGGCCTAGGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGCCTGTCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGAcgc                         <  2:457798/149‑1 (MQ=255)
                                                                                                                             ttATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGAcgc                         <  1:1276774/149‑1 (MQ=40)
                                                                                                                             ttATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGAcgc                         <  2:622776/149‑1 (MQ=40)
                                                                                                                              tATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGCTAAGAcgcg                        >  2:8347/1‑149 (MQ=255)
                                                                                                                              tATCCGGCCTACGGGTGGCGCGAGAAGGTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGCGCGGGAATTTGTAGGCCTGATACGAcgcc                        >  2:1660813/1‑148 (MQ=255)
                                                                                                                               aTCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTATGGATGGCGCGGGAATTTGTAGGCCTGATAAGa                            <  1:27719/144‑1 (MQ=255)
                                                                                                                               aTCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTATGGATGGCGCGGGAATTTGTAGGCCTGATAAGa                            >  2:27719/1‑144 (MQ=25)
                                                                                                                                  cGGCCTACGGGTGGCGCGAGAATTTATAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTATGGAGTGCGCGGGAATTTGTAGACCCGATAAGACGCGTTAg                    <  1:1333846/149‑1 (MQ=14)
                                                                                                                                    gCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGAAGCGTTAGCg                  >  1:292129/1‑149 (MQ=255)
                                                                                                                                     ccTACGGGTGGCCAGAGAATTTGTAGGTCTGATAAGATGCGTTACCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGt                 <  1:824861/149‑1 (MQ=11)
                                                                                                                                      ctgcGGGTGGCGGGAGAATGTGTAGGCCTGATAAGCCGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTc                <  2:898405/146‑1 (MQ=255)
                                                                                                                                        aCGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGc              <  1:1379091/149‑1 (MQ=40)
                                                                                                                                        aCGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCGGATCAGACGCGgtcgcgtcgc              >  1:538687/1‑149 (MQ=255)
                                                                                                                                        aCGGATGGCGCGGGAATTTGTAGGCCTGATGAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGc              >  1:944299/1‑149 (MQ=2)
                                                                                                                                            gTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGGTAGCAGCGCATCa          >  1:116743/1‑149 (MQ=255)
                                                                                                                                                cgcgAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGTGGCGTAAACGCCTTAt                                                                     <  1:1447275/86‑1 (MQ=255)
                                                                                                                                                cgcgAGAATATGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTCGTAGGCCTGATAAGACGCGTCGGCGTCGCATCAGGCa      >  2:843053/1‑149 (MQ=255)
                                                                                                                                                 gcgAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCGTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCAt     <  1:251724/149‑1 (MQ=37)
                                                                                                                                                  cgAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCagc    >  1:369211/1‑147 (MQ=25)
                                                                                                                                                  cgAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATc    <  2:81904/149‑1 (MQ=35)
                                                                                                                                                   gagaATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTGGCATCAGGCACCt   >  2:1066746/1‑149 (MQ=25)
                                                                                                                                                   gagaATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCt   >  2:922126/1‑149 (MQ=35)
                                                                                                                                                    |                                                                                                                                                    
ACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTG  >  NC_000913/375028‑375324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: