Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,093,040 G→A A215T (GCT→ACT)  hisC → histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,093,0400GA98.3% 181.5 / NA 60A215T (GCT→ACT) hisChistidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (31/27);  minor base C (0/1);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATC  >  NC_000913/2092907‑2093183
                                                                                                                                     |                                                                                                                                                
ccTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCgg                                                                                                                                  >  1:940560/1‑149 (MQ=255)
 ctgctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCggg                                                                                                                                 <  2:784305/149‑1 (MQ=255)
     tgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCtt                                                                                                                             >  1:965624/1‑149 (MQ=255)
       gAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCCGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTATCGACAACTTTTCCTCTGGCGGGGCCTCg                                                                                                                           >  1:720869/1‑149 (MQ=255)
       gAGTTAACCCGCGGTAAGGCGAGTGTGGTTGCCGATGAAGCCTATATAGAGTTTTGCCCGCAGGCATCGCGGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTCTTTTACGCACCCTGCCGGAAACTTTGGCTCTGGGGGGGCTTCg                                                                                                                           >  2:470541/1‑149 (MQ=255)
         gTTAACCCGCCGTAACGCGATTGTGGTTGCCGATGAAGCCTATAACGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGtt                                                                                                                         <  1:632680/149‑1 (MQ=255)
             aCCCGCGGTAAGGCGATTGTGGTTGACGATGAAGCCTATATCGAGTTATGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTTGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCgg                                                                                                                     >  1:612901/1‑149 (MQ=255)
               ccGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGAt                                                                                                                   >  2:1012095/1‑149 (MQ=255)
                      aaGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCt                                                                                                            >  1:219635/1‑149 (MQ=255)
                      aaGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCt                                                                                                            <  1:628074/149‑1 (MQ=255)
                       aGGCGATTGGGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTg                                                                                                           <  1:160870/149‑1 (MQ=255)
                         gCGATTGGGGTTGCCGATGAAGCCTATAACGCGTTTGGCCCGCGGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGc                                                                                                         >  2:461941/1‑149 (MQ=255)
                           gATTGTGCTTGCCGATGATGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCaa                                                                                                       <  1:16428/149‑1 (MQ=255)
                                tgGTTGCCGATGAAGCCTACACCGAGTTTTGCCCGCAGGCATCGCTGCCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaa                                                                                                  <  2:259573/149‑1 (MQ=255)
                                  gTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaaga                                                                                                >  1:1026657/1‑149 (MQ=255)
                                   ttGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                               <  1:500153/149‑1 (MQ=255)
                                        gATGAAGCCTATATCGAGTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGtcatc                                                                                         <  1:341541/149‑1 (MQ=255)
                                               ccTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACctg                                                                                   <  1:671129/149‑1 (MQ=255)
                                                  atatCGAGTTTTGCCCGCAGGCATCTCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACctgctg                                                                                <  1:1012095/149‑1 (MQ=255)
                                                  atatCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGACTCTGGGTTGTGCTGATTTAAGCTGGCGGACGAAGAAGTCATCAACctgctg                                                                                >  1:790518/1‑149 (MQ=255)
                                                  atatCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACctgctg                                                                                <  2:925666/149‑1 (MQ=255)
                                                   catCGAGTTTTGCCCGCGGGCACCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCtga                                                                               <  2:592477/148‑1 (MQ=255)
                                                    atCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGCAGGAGTCATCAACCTGCcgat                                                                              >  1:39229/1‑149 (MQ=255)
                                                        aGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGaaa                                                                          <  1:503219/149‑1 (MQ=255)
                                                               cccGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGGCATCAACCTGCTGATGAAAGTGATCg                                                                   >  1:693347/1‑149 (MQ=255)
                                                                ccGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGc                                                                  >  2:789631/1‑149 (MQ=255)
                                                                 cGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGcc                                                                 >  2:779321/1‑149 (MQ=255)
                                                                  gCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGAGTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGccc                                                                >  1:846693/1‑149 (MQ=255)
                                                                  gCAGGCATCGCTGGCTGGCGGGCTGGCCGAACATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGAGTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGccc                                                                <  2:486280/149‑1 (MQ=255)
                                                                   cAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGACTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGcccc                                                               <  1:752651/149‑1 (MQ=255)
                                                                      gCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGGTGAAAGTGATCGCCGCCTa                                                            >  2:215562/1‑149 (MQ=255)
                                                                        aTCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTAcc                                                          <  1:395931/149‑1 (MQ=255)
                                                                                 tggctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGGGATCGCCCCCTACCCGCTCCCGa                                                 >  2:692469/1‑149 (MQ=255)
                                                                                 tggctggccggcGGAATATCCGCACCTGGCTAATTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGTATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGa                                                 <  2:612709/149‑1 (MQ=255)
                                                                                     tggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGcc                                             >  2:241745/1‑149 (MQ=255)
                                                                                       gctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCgg                                           >  1:510469/1‑149 (MQ=255)
                                                                                         tggcTGAATCTCCGCTCCTGCCTATTTTCCGCACCCTGGCGAAAACTTTCGCTCTGGCGGGGCTTCGTTCCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGtt                                         <  1:129343/149‑1 (MQ=255)
                                                                                          ggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGTCGGGGCTTCGTTGCGGATTTACGCTGGCGAACGAAGAAGTCATCAACCTGCCGATGCAAGTGCTCGCCCCCTACCCGCTCTCGCCGCCTGTTg                                        >  2:3415/1‑149 (MQ=255)
                                                                                           gccgAATATCCGCACCTGGCTATCTTACGCACACTGTCGAAACCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGc                                       <  2:184542/146‑1 (MQ=255)
                                                                                           gcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGc                                       >  1:918239/1‑149 (MQ=255)
                                                                                            cGGAATATCCGCACCTGGCTATTTTACGCCCACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGACGAAGTCAGCAACCTGCTGCTGAAAGTGATCGCCCACTACCCGCTCTCGCCGCCGGTCGcc                                      >  2:308983/1‑149 (MQ=255)
                                                                                              gAACATCCGCACCTGTCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGCCATCAACCTGCTGCTGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGa                                    <  2:254109/149‑1 (MQ=255)
                                                                                                atatCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACa                                  >  1:1091268/1‑149 (MQ=255)
                                                                                                atatCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACa                                  <  1:461941/149‑1 (MQ=255)
                                                                                                   tCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTg                               <  2:588806/149‑1 (MQ=255)
                                                                                                     cGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCg                             <  1:599851/149‑1 (MQ=255)
                                                                                                     cGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATGTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCg                             <  2:790518/149‑1 (MQ=255)
                                                                                                      gCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTCCCCGCACTCTACGCCGGTTGCCGACATTGCgg                            >  2:226885/1‑149 (MQ=255)
                                                                                                          cTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCa                        <  2:510469/149‑1 (MQ=255)
                                                                                                            ggCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAgg                      <  2:792827/149‑1 (MQ=255)
                                                                                                             gCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATGTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGc                     >  1:310984/1‑149 (MQ=255)
                                                                                                                aTTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGtt                  >  1:541904/1‑149 (MQ=255)
                                                                                                                aTTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGtt                  >  1:577314/1‑149 (MQ=255)
                                                                                                                 ttttACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTa                 >  1:714600/1‑149 (MQ=255)
                                                                                                                 ttttACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTa                 <  1:271853/149‑1 (MQ=255)
                                                                                                                    tACGCACACTGTCGAAAACTTGTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGc              <  1:214858/149‑1 (MQ=255)
                                                                                                                         acagTGTCTATATCTTTGGCTCTGGCGGGGCTTCGTTTCGGATTTACGCTGTTTAACAAAGAAGTCATCAATCTGCTTTTGAGCGTGGTCGCCCCCTGCCCGCTCTCGACGTCGGTTGCCGACATTGCGGCTCTGTCGTGAAGCCcaca         <  2:467519/149‑1 (MQ=255)
                                                                                                                             tGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGCCGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGa     >  1:26320/1‑149 (MQ=255)
                                                                                                                               tCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAt   >  1:949834/1‑149 (MQ=255)
                                                                                                                               tCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAt   >  1:693567/1‑149 (MQ=255)
                                                                                                                                caaaaaCTTTTTCCCGGGCGGGCCTTCGTTGCGGATTTACGCTGGCAACCGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtc  <  1:71164/147‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                                
CCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATC  >  NC_000913/2092907‑2093183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: