Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 257,908 Δ776 bp 100% [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908 258683 776 44 [0] [0] 44 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)39 (1.010) 33/260 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

CGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913/257760‑258055
                                                                                                                                                   |                                                                                                                                                    
gtcttGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTGACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAa                                                                                                                                                     <  2:79949‑M1/147‑2 (MQ=255)
   ttGTGTTAAAAAATTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCAACACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtg                                                                                                                                                  <  1:115693‑M1/149‑5 (MQ=255)
           aaaaTTTGCTAAATTTTGCCAATTTGGTAAACAAGTTGAATGACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagata                                                                                                                                          >  1:723215‑M1/1‑137 (MQ=255)
           aaaaTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGAAagtgcaaagata                                                                                                                                          >  2:179339‑M1/1‑137 (MQ=255)
                    tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGCAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCACGTATATTCGTGAAGGCAagtgcaaagataaccgattct                                                                                                                                 >  2:684671‑M1/1‑128 (MQ=255)
                               aaTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgt                                                                                                                      >  2:511075‑M1/1‑117 (MQ=255)
                                  ttGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctg                                                                                                                   <  1:130667‑M1/149‑36 (MQ=255)
                                   tGGTAAATCAGTTGCATAAACACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgg                                                                                                                  <  1:529302‑M1/149‑37 (MQ=255)
                                       aaaaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgt                                                                                                              >  1:353226‑M1/1‑109 (MQ=255)
                                        aaaCAGCTTAATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCCCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgta                                                                                                             <  1:64255‑M1/149‑42 (MQ=255)
                                          aCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtgtg                                                                                                           >  1:706913‑M1/1‑106 (MQ=255)
                                           cAGTTCCATCACACCAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCTCACTAGGCCCGTATATTCGTGAAGGTAattgcaaagataatcgattctttttcgattgtctggctgtatgc                                                                                                          <  2:533456‑M1/149‑45 (MQ=255)
                                            aGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATCCGCGCGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcg                                                                                                         >  1:568027‑M1/1‑104 (MQ=255)
                                                  aacacaacAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAACTTTACCGCACTAGGCCCGTATATTCGTTAAGGTAagtgcaaagataatcgattctttttcgattgtctggatgtatgcgtcaacg                                                                                                   <  2:271137‑M1/147‑52 (MQ=255)
                                                   tCACAACAGGAGATAGCAATGCAGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagatattcgattctttttcgattgtctggctgtatgcgtcaacgt                                                                                                  <  2:205617‑M1/149‑53 (MQ=255)
                                                    cacaACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATGAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagagcaaagataatcgattctttatcgatggtctggctgtaggcgtgaacgtg                                                                                                 >  2:469147‑M1/1‑96 (MQ=255)
                                                     aaaacaggATATAGACATGACGTTAACCAGTGGACATCTGAAGAGCAGATTGATCAAAGAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattttttttcgattgtctggctgtatgcgtcaacgtga                                                                                                <  1:341451‑M1/147‑55 (MQ=255)
                                                        acaGGAGAAAGGAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATAAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaac                                                                                             <  2:269028‑M1/149‑58 (MQ=255)
                                                         caGGAGATAGCAATGACGCTACCGAGGGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCCCCCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgtttctggttcgattgtctggctgtatgcgtcaacgtgaaacc                                                                                            <  2:307367‑M1/149‑59 (MQ=255)
                                                                   cAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaaaataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaa                                                                                  <  1:688672‑M1/149‑69 (MQ=255)
                                                                    aaTGACGTTACCGAGTGGGCACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaag                                                                                 <  2:653987‑M1/149‑70 (MQ=255)
                                                                    aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaag                                                                                 <  2:653992‑M1/149‑70 (MQ=255)
                                                                    aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaag                                                                                 >  1:70252‑M1/1‑80 (MQ=255)
                                                                    aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaag                                                                                 <  1:704501‑M1/149‑70 (MQ=255)
                                                                           ttACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtga                                                                          >  1:315334‑M1/1‑73 (MQ=255)
                                                                              ccGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgcatt                                                                       >  2:544291‑M1/1‑70 (MQ=255)
                                                                                 aggggacaCCCGAAGAGCAGATTGATCAAAAAATGTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctg                                                                    <  2:634683‑M1/146‑83 (MQ=255)
                                                                                        acCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTCTATTCGTGAAAGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaatactggggctgg                                                             >  2:4015‑M1/1‑60 (MQ=255)
                                                                                        acCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctgg                                                             >  1:697745‑M1/1‑60 (MQ=255)
                                                                                              agggcagATTGATCAAAAAATTTACCTCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatg                                                       <  2:696851‑M1/146‑96 (MQ=255)
                                                                                                  cAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagc                                                   >  1:387605‑M1/1‑50 (MQ=255)
                                                                                                   aGATTGATCAAAAAATTTACCGCACTAGGCCCGTCAACTCGAATATGCTattgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggccgtgcgtgaattatggggctgctggatggagat                                                  <  2:515255‑M1/149‑101 (MQ=255)
                                                                                                    gATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagatagtcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcactggaagtgggtgaattctggggatggtgggtggggctg                                                 >  2:389796‑M1/1‑48 (MQ=255)
                                                                                                     aTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgg                                                <  1:100448‑M1/149‑103 (MQ=255)
                                                                                                      ttGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTGagtgcaaagctagtcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgga                                               >  2:3341‑M1/1‑46 (MQ=255)
                                                                                                            aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                         <  1:511075‑M1/149‑110 (MQ=255)
                                                                                                             aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcag                                        <  1:87159‑M1/149‑111 (MQ=255)
                                                                                                                                 ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcagggatcccgttttacctacag                    >  2:608723‑M1/1‑19 (MQ=255)
                                                                                                                                    tataTGCGTGAAGGTAagtgcaaagataatcgaatctttttcgattgtgtggctgtatgcgacaacgtgaaaccggcaccggaagtgcgtgcattctggggctggtggatggagctggaagcgcgggaatcccgatgaacctaaagttg                 >  2:82243‑M1/1‑16 (MQ=255)
                                                                                                                                     atatTCGTGAAGGTAagtgcaaagatactcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattccggggctggtggatggagcgggaagcgcaggaatcccgttttacctacagttac                <  1:510059‑M1/149‑135 (MQ=255)
                                                                                                                                     atatGCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttac                <  1:311881‑M1/149‑135 (MQ=255)
                                                                                                                                            tGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttg         <  1:284574‑M1/149‑142 (MQ=255)
                                                                                                                                                 ggaagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctg    <  2:464929‑M1/147‑147 (MQ=255)
                                                                                                                                                   aagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgtt  <  1:474186‑M1/149‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATT  >  NC_000913/257760‑258055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.