Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,451,102 C→T 8.5% A161T (GCC→ACC)  yfcR ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,451,1020CT 8.5% 121.1 / 3.5 47A161T (GCC→ACC) yfcRputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (20/23);  new base T (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.19e-01

GATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATG  >  NC_000913/2450963‑2451247
                                                                                                                                           |                                                                                                                                                 
gATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTc                                                                                                                                          <  2:67289/149‑1 (MQ=255)
   gCTACAGGTTGGCGGTGACAATTATGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGcc                                                                                                                                       <  1:462008/149‑1 (MQ=255)
            tGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGCTAAACTCGCCCGCAGCAAg                                                                                                                              <  1:531502/149‑1 (MQ=255)
             ggCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGt                                                                                                                             <  2:537764/149‑1 (MQ=255)
                      aataatGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCGAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCg                                                                                                                    >  2:669215/1‑149 (MQ=255)
                      aataatGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAACATCGCCCGCCGCACGTTGCGCCCCg                                                                                                                    >  2:532081/1‑149 (MQ=255)
                        taatGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAGGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCCAGTTGCGCCCCGCt                                                                                                                  >  1:303144/1‑149 (MQ=255)
                        taatGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGCTAACCTCGCCCCCAGCAAGTTGCGCCCCGCt                                                                                                                  >  2:421690/1‑149 (MQ=255)
                         aatGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCtt                                                                                                                 <  2:201952/149‑1 (MQ=255)
                          atGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAATCTCGCCCGCAGCAAGTTGCGCCCCGCTTg                                                                                                                <  2:352045/149‑1 (MQ=255)
                             tCCCGTGAAAGGTGATATCTTCATCAGCAGCATGAGCTGTGGCGCTACTTACACAAAGCAGGGAGAAGAAAGTCGTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCt                                                                                                             >  2:176310/1‑149 (MQ=255)
                               ccGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGCCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTc                                                                                                           <  2:662033/149‑1 (MQ=255)
                                             ggcTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCtt                                                                                             <  2:64094/147‑1 (MQ=255)
                                             gTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCtt                                                                                             >  1:432707/1‑149 (MQ=255)
                                                   atcaGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCACGAAGCAAGTTGCGCCCCGCTTGCCTTCAAAGGTACAGCCTTCAACtg                                                                                       >  2:10440/1‑149 (MQ=255)
                                                    tcaGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACtgt                                                                                      >  1:453396/1‑149 (MQ=255)
                                                     cagcagCATGAGCCATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAACCTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACtgtg                                                                                     <  1:107084/149‑1 (MQ=255)
                                                         agcaTGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCTGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCCACTGTGGCTg                                                                                 <  2:200279/149‑1 (MQ=255)
                                                         agcaCGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTg                                                                                 <  2:260275/149‑1 (MQ=255)
                                                          gtatcaGCTATGGCGCCACTGACACAGATCATGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTgg                                                                                <  1:494212/144‑1 (MQ=255)
                                                                  tATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTATTGat                                                                        <  1:151993/149‑1 (MQ=255)
                                                                      gcgcTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTATTGATATTa                                                                    >  1:259438/1‑149 (MQ=255)
                                                                            cTGACACCAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCCGCAAGTGGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTATTGATATTAAATGGc                                                              <  2:183645/149‑1 (MQ=255)
                                                                            cTGACACAAAGCAGGGTTAAGAAAGTCTTTCTGATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTATTGATATTAAATGGc                                                              <  1:499358/149‑1 (MQ=255)
                                                                             tGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTATTGATATTAAATGGCg                                                             <  1:65871/149‑1 (MQ=255)
                                                                                 acaAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTCTTGATATTAAATGGCGCCCa                                                         >  2:353728/1‑149 (MQ=255)
                                                                                    aaGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAAATAGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTGCAAGTGTGGCGGGTTATTGATATGAAATGGCGTCCAGCt                                                      >  1:426554/1‑149 (MQ=255)
                                                                                          ggTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTAGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTATTGATCTTAAATGGCGTCAGGCTATTTTc                                                >  1:309233/1‑149 (MQ=255)
                                                                                            tcaaGAAAGTCTTTCTCATCGATAGTCCACCACCATGCTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTTCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCAc                                              <  2:81653/147‑1 (MQ=255)
                                                                                                gAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGAc                                          >  1:296321/1‑149 (MQ=255)
                                                                                                gAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTAAACTGTGGCTGGTTATTGAAAGTAGATGGCGTCCAGCTATTTTCACCGAc                                          >  2:433336/1‑149 (MQ=255)
                                                                                                 aaaGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGaag                                         <  1:332386/149‑3 (MQ=255)
                                                                                                 aaaGTCCTTCTCAATGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCCTCCACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACg                                         <  1:572430/149‑1 (MQ=255)
                                                                                                       tttCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACCGCCTTCAACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAAc                                   >  1:19884/1‑149 (MQ=255)
                                                                                                       tttCTCATTGAAAGACGACAACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGCCTGGTTATGGATATTAAATGGCGTCCCGCCATTTTCACCGACGACAGAc                                   >  2:270478/1‑149 (MQ=255)
                                                                                                           tcATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCATCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTCTTGATATTAAATGGCGCCCAGCTATTTTCACCGACGGCAAACAATg                               >  1:678257/1‑149 (MQ=255)
                                                                                                            cATTGATAGTGCACCACCATGGGCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGa                              <  1:626984/149‑1 (MQ=255)
                                                                                                                  tAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATc                        >  1:355790/1‑149 (MQ=255)
                                                                                                                        accaccaTGGTCAAACTGGTGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTgg                                                                                    >  2:417396/1‑83 (MQ=255)
                                                                                                                        accaccaTGGTCAAACTGGTGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTgg                                                                                    <  1:54986/83‑1 (MQ=255)
                                                                                                                        accaccaTGGTCAAACTGGTGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTgg                                                                                    >  2:54986/1‑83 (MQ=255)
                                                                                                                        accaccaTGGTCAAACTGGTGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTgg                                                                                    <  1:417396/83‑1 (MQ=255)
                                                                                                                                 gTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTATTGATAGTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTAt         <  1:511221/149‑1 (MQ=255)
                                                                                                                                  tCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATc        <  1:580140/149‑1 (MQ=255)
                                                                                                                                  tCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATc        >  2:100839/1‑149 (MQ=255)
                                                                                                                                     aaCTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCAGGATATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACCATGGGTTATAGGCGCTATTTTCTATCCGa     >  1:96646/1‑149 (MQ=255)
                                                                                                                                        cggCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGTATg  <  2:15110/148‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                 
GATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCACCATGGTCAAACTGGCGTTAAACTCGCCCGCAGCAAGTTGCGCCCCGCTTGCCTTCACAGGTACAGCCTTCAACTGTGGCTGGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATG  >  NC_000913/2450963‑2451247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑