| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,913,421 | G→A | 17.3% | R77W (CGG→TGG) | relA ← | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,913,421 | 0 | G | A | 17.3% | 83.1 / 8.0 | 46 | R77W (CGG→TGG) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Reads supporting (aligned to +/- strand): ref base G (12/25); new base A (3/5); total (15/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCT > NC_000913/2913277‑2913564 | cAGTGTGCGTCGCTTTCAGCTGGTGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGc < 2:376395/149‑1 (MQ=255)cAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCGGAGGGAAAAGCAGCGCCGCCCGCAGc > 1:641806/1‑149 (MQ=255) gtgtGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCgt < 1:693433/149‑1 (MQ=255) gtgtGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCgt < 1:74508/149‑1 (MQ=255) gtgCGTCGCTTTCAGCTGGCGGATCGCCGCCATAACACGAACGCCCTGAATAAGGGGAACGACCGACTCACCGACGCTCTCACGCAGCACATCTCCGCTGACTACGTTGGCATCCGCAAGAGGGAAAACCAGCGCCCCCCACAGCgctt > 2:28292/1‑146 (MQ=255) gtgcgtttCAGCTGGCTGATCCCCGCCATATACGGCACGCCGTGAATAAGGTTAACTACCGAGTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTGGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAAt < 2:20590/144‑1 (MQ=255) gTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCACAGCGTGTCAAt > 1:82095/1‑149 (MQ=255) tttCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCa > 2:366596/1‑149 (MQ=255) tttCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACCACGCTGGCATCCGCCATAGGGAAACGCAGCGCCGCCCGCAGCGTGTCAATGTCCa < 1:548632/149‑1 (MQ=255) cAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATAc < 1:578563/149‑1 (MQ=255) aGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAACGCCCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAAGGTCCATACt > 1:93721/1‑149 (MQ=255) cgATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTc < 1:124854/148‑1 (MQ=255) ccgccATATCACGCACGCCGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAgg > 1:575525/1‑149 (MQ=255) cATATCACGCACGCCGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGAtct < 1:302827/149‑1 (MQ=255) gcCGTTAATGAGGTTAACTCCCGACTTCCCGACGCCCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCTCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCaa < 2:30064/149‑1 (MQ=255) gcCGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCaa > 1:135387/1‑149 (MQ=255) tAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGCGGATCTCCACCATCTCAACACCACGc > 1:2312/1‑149 (MQ=255) ggTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCCCAACACCCCGCcac > 2:243466/1‑149 (MQ=255) ggTGAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCcac < 1:236384/149‑1 (MQ=255) gaccgacTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACa > 1:395670/1‑149 (MQ=255) ccgacTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGa < 1:127735/149‑1 (MQ=255) acTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCACTGCCCATCTAGAAGTTCGAGAGGATCTCCACCACCCCAACACCACGCCACAAT‑AACAGACTg > 1:321612/1‑149 (MQ=255) ttACCGACCCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGc < 2:651731/149‑1 (MQ=255) tACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGGCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCa < 1:505270/149‑1 (MQ=255) cGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCATCCGGa < 1:614348/149‑1 (MQ=255) cGCTCTCACGCAGCACATCTCCGCTGACTACGTTGGCATCCGGCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCATCCGGa < 1:682539/149‑1 (MQ=255) gCTCTGACGAACCACCTCTTCGCTGACTCCGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCACAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGTATCCGGAt < 2:93043/149‑1 (MQ=255) cccccgcaCATCTTCGCGGACTACGTTGCCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCATCCGGATGCCCCt < 2:341387/144‑1 (MQ=255) agcaCATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCATCCGGATGCCCCTGCGt < 1:393443/149‑1 (MQ=255) acaTCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTAAACACCACGCCACAAT‑AACAGACTGGCATCCGGATGCCCCTGCGTCTg < 2:261478/149‑1 (MQ=255) acaTCGTCGCTGACTACGTGGGCATCCGCCAGAGGGAAAAGCCGCGCCGCCCGCAGCGTGGCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTAAACACCACGCCACAAT‑AACAGACTGGCATCCGGATGCCCCTGCGTCTg < 2:261377/149‑1 (MQ=255) cTTCGGTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGc < 2:272568/149‑1 (MQ=255) cTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGc > 1:168383/1‑149 (MQ=255) cTTCGCTGACTACGTTGGCATCCGCAAGAGGGAAAAGCAGCGCCGCCCACAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGc < 2:640654/149‑1 (MQ=255) ttCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCa < 2:395670/149‑1 (MQ=255) acgaCGTTGGCATCCGCCAGAGGGAAAAGCCGCGCCGCCCGGAGCGTGTCATTGTCCATACTCAATGGCGAGAGAATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCTGACAATa < 2:310636/146‑1 (MQ=255) gTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCATCCGGATGCCCGTGCGTCTGTTGCAGACAATACGccc < 1:366596/149‑1 (MQ=255) gtGGCATCCGCCAGAGGGAACAGCAGCGCCGCCCACAGCGTGTCAATGTACATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCa < 2:481152/148‑1 (MQ=255) cATCCGCCAGAGGGAAAAGCAGCGCCGCCCACAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGtt > 2:538171/1‑149 (MQ=255) tCCGCCAGAGGGAAAAGCAGCGCCGCCCGAAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCCTCCGGATGCCCCTGCGCCTGTTGCAGACACAACTCCCAgcgttc > 2:333997/1‑144 (MQ=255) agagGGAAAAGCAGCGCCGCCCACAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAGCACCACGCCACAAT‑AACCGACTGGCATCCGGATGCCCCTGCGTCTGTTGCTGACAATACGCCCAGGTTTCGGCTaa < 2:344090/149‑1 (MQ=255) gAAAAGCAGCGCCGCCCGCCGCGTGTCAATGTCCATACTTAATGTCGTGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACt < 2:624862/149‑1 (MQ=255) gAAAAGCAGCGCCACCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGATTTCGGCTAAGCAcc > 2:384451/1‑148 (MQ=255) aaGCAGCGCCGCCCACAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTcac < 1:60499/149‑1 (MQ=255) aaGCAGCCCCGCCCGCAGCGTGTCAATGTCCATACTTCATGTCGCGAGGCTCTCCACCATCTCAACACCACGCCACAATCCACCG‑GTGCCATCCGGATGCTCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTcac < 1:426899/149‑1 (MQ=255) agcGCCCCCCGCAGCCTGTCAGTGTCCATATTTAATTTCGTGAGGATATCTGCCATCTCAACACCCCGCGACAAT‑AGGAGTCTGGCAGCCTGATGCCCTTGCTTCTGTTGCAGACAATACGCACAGGTTTCGGCTAAGCACTCACACGa < 2:38903/149‑1 (MQ=255) ccgcccacAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCCACACCACGCCACAAT‑AACAGACTGGCATCCGGATGCCCCTCCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTc > 1:702917/1‑149 (MQ=255) ggaccgcAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTAAACACCACGCCACAAT‑CACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACttat < 2:210594/146‑3 (MQ=255) | CAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAAT‑AACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCT > NC_000913/2913277‑2913564 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |