Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,413,542 A→T 100% Y67N (TAT→AAT)  ydaC ← DUF1187 family protein, Rac prophage; putative double‑strand break reduction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,413,5420AT100.0% 183.1 / NA 56Y67N (TAT→AAT) ydaCDUF1187 family protein, Rac prophage; putative double‑strand break reduction protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (28/28);  total (28/28)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGAAGCATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGT  >  NC_000913/1413397‑1413686
                                                                                                                                                 |                                                                                                                                                
cGAAGCATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCaa                                                                                                                                               >  1:599662/1‑149 (MQ=255)
  aaGCATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATg                                                                                                                                             <  1:19988/149‑1 (MQ=255)
      aTCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAAt                                                                                                                                         >  1:3548/1‑149 (MQ=255)
          ggCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTACTAATCAGCTATGATGTTTTAATTTGAATTCAATGCAATtctc                                                                                                                                     >  2:254524/1‑149 (MQ=255)
            ccGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATTGGGATTATTACTAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGa                                                                                                                                   <  2:701639/149‑1 (MQ=255)
             cGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAg                                                                                                                                  >  1:284277/1‑149 (MQ=255)
               tATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCGGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTTTCGAgta                                                                                                                                >  1:610997/1‑147 (MQ=255)
                    aaCCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCCCTGGCAGGCGCCAAGATTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTCTTAATTTGAATTCAATGCAATTCTCGAGGACTGaa                                                                                                                           >  2:6181/1‑149 (MQ=255)
                            aCTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCg                                                                                                                   <  1:187943/149‑1 (MQ=255)
                                     tAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTaaaa                                                                                                          >  1:338860/1‑149 (MQ=255)
                                      acaGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAAt                                                                                                         <  1:306178/147‑1 (MQ=255)
                                               ttGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATACTCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGtt                                                                                                >  2:693473/1‑149 (MQ=255)
                                               ttGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGCAGTTTCTCGCAATTAAAATTTATCatgt                                                                                                >  2:324524/1‑146 (MQ=255)
                                                  tgCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTa                                                                                             <  1:339626/149‑1 (MQ=255)
                                                   gCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATGTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTAc                                                                                            <  1:63726/149‑1 (MQ=255)
                                                     cATCGCATCACCGCTGGCAGGCTCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTTAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAACTTTATCAGTTTTACtt                                                                                          >  2:204552/1‑149 (MQ=255)
                                                      aTCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACttt                                                                                         >  2:650208/1‑149 (MQ=255)
                                                          cATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAAATCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGc                                                                                     <  1:144772/149‑1 (MQ=255)
                                                            tCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGctc                                                                                   >  2:616713/1‑149 (MQ=255)
                                                                ctctGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGGTTTACTTTCTGCTCTCTg                                                                               <  1:538484/149‑1 (MQ=255)
                                                                  ctGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGa                                                                             >  1:290802/1‑149 (MQ=255)
                                                                   tGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGaa                                                                            >  1:485672/1‑149 (MQ=255)
                                                                     gCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGaaag                                                                          >  1:595271/1‑148 (MQ=255)
                                                                              aaTGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGcttc                                                                 <  1:465156/149‑1 (MQ=255)
                                                                               aTGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTTTCTGGAAACGCCTGcttct                                                                >  2:449446/1‑149 (MQ=255)
                                                                                    aGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCtttttt                                                           <  2:289568/149‑1 (MQ=255)
                                                                                        tGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCt                                                       >  2:459157/1‑149 (MQ=255)
                                                                                        tGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATCCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCt                                                       >  1:685265/1‑149 (MQ=255)
                                                                                          aaaTTGACGCCCGATGTTGATTAGTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTga                                                     <  2:271059/149‑1 (MQ=255)
                                                                                          aaaTTGAACCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTga                                                     <  2:453518/149‑1 (MQ=255)
                                                                                           aaTTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTgag                                                    >  2:213438/1‑149 (MQ=255)
                                                                                            aTTGACGCCCGATGTTTATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTgaga                                                   <  1:124144/149‑1 (MQ=255)
                                                                                             ttGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGagag                                                  >  1:253527/1‑149 (MQ=255)
                                                                                                  gCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGATCAttt                                             >  2:455587/1‑149 (MQ=255)
                                                                                                  gCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCAttt                                             >  1:483844/1‑149 (MQ=255)
                                                                                                   cccGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTAGTTGAGAGCAtttt                                            >  1:458169/1‑149 (MQ=255)
                                                                                                            gATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCgttttc                                   >  2:31190/1‑144 (MQ=255)
                                                                                                            gATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTc                                   >  1:388061/1‑149 (MQ=255)
                                                                                                             attattAATAATCAGCTATTAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCCCTCTGGAAACGCCTGCTTCTTTTTGACCTGAGAGCATTTTTTCGCATTCt                                  <  2:316820/149‑1 (MQ=255)
                                                                                                                 ttaATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGAtt                              <  2:338396/149‑1 (MQ=255)
                                                                                                                  taataaTCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGAttt                             <  1:406121/149‑1 (MQ=255)
                                                                                                                  taataaTCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGAttt                             <  1:614091/149‑1 (MQ=255)
                                                                                                                  taataaTCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGAttt                             >  1:297283/1‑149 (MQ=255)
                                                                                                                   aataagCAGCTATGAAGGTTTAATTTGAATTACATGCAATTCTCGCGTACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCGGAGAGCATTTTTTCGCATTCTGATTTc                            <  2:44360/149‑1 (MQ=255)
                                                                                                                       atCAGCTTTGCAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTa                        <  1:441599/149‑1 (MQ=255)
                                                                                                                             tATGAAGTTTTAATTTGAATTCCATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAAGTTATCAGTTTTCCTTTCTGCTCTCTGGACACGCCTGCATCTTTTTTCCCTGAGATCATTTTTCCGCATTCTGATTTAGTTAGTTTAg                  <  2:121994/149‑1 (MQ=255)
                                                                                                                                 aaGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGAttt              <  1:697119/149‑1 (MQ=255)
                                                                                                                                  aGTTTTAATTTGAATTCAATGTCACTCCCGAGGACTGACGTCACTTAAAGGTGGTATTTATCAGTTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGAtttt             <  1:347963/149‑1 (MQ=255)
                                                                                                                                   gTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTg            <  1:650208/149‑1 (MQ=255)
                                                                                                                                    ttttAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGa           >  2:383132/1‑149 (MQ=255)
                                                                                                                                    ttttAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCCGGGAACGCCTTCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGa           <  1:21937/149‑1 (MQ=255)
                                                                                                                                     tttAATTTGAATTCAATGCAATTCTCGAGGACTTAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAACCGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGaa          <  1:65767/149‑1 (MQ=255)
                                                                                                                                     tttAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGaa          <  2:178017/149‑1 (MQ=255)
                                                                                                                                     tttAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGaa          <  1:143532/149‑1 (MQ=255)
                                                                                                                                            tGAATTCAATGCAATCTTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTg   <  2:277690/149‑1 (MQ=255)
                                                                                                                                             gAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGt  >  1:584835/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGAAGCATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGT  >  NC_000913/1413397‑1413686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑