Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,019,613	Δ1 bp	7.5%	intergenic (+100/‑5)	fliK → / → fliL	flagellar hook‑length control protein/flagellar biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,019,613	0	A	.	7.5%	174.4 / 6.8	53	intergenic (+100/‑5)	fliK/fliL	flagellar hook‑length control protein/flagellar biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (25/24);  new base . (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

AAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATCGCGTACTTTATATCGGCATAACCCTGCGCCTGAAAGATGAAGCTACCCGC  >  NC_000913/2019336‑2019878                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                           aaGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCAGCGTCTTTTCCCCGCTTTGTTGCGCGCAAGACGCCGGATAATTAGCGGCCAAGCAGTAGAGACACAGGAAAACAGCaa                                                                                                                                                                                                                                                                           >  1:619/1‑279 (MQ=255)                   aaGCTTTAGTGGGCAGCAGAAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGGCAGAGGTAGCACCGTAATCCGCGGCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGAGAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGAt                                                                                                                                                                                                                                                               <  2:564909/273‑1 (MQ=255)                                                         agcaaAGACAACGCACAGCAAACCAGGAGCCTCTGTCGTGGGAAGACGACGATACGCTTCCGGTTCCAGTCTCTTTACAAGGGCGTGGAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATcc                                                                                                                                                                                                                       <  2:43290/275‑1 (MQ=255)                                                          gcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCCGGATAATTAGCCGCATAGCAGTAGCGACACAGGAAGACCGCAAAACATGACTGATTACGCGATAAGCACAAAAAGCAAGCGAACGCTTTGAAcccc                                                                                                                                                                                                                      >  4:209260/1‑275 (MQ=255)                                                                    tgCACAGCAAACCATGAACCTCTGGCGGGGGAAGACAACGCTACGCTTCCGGTTCCCGTCTCTTTCCAATGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTa                                                                                                                                                                                                            <  2:589441/274‑1 (MQ=255)                                                                    cGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTgg                                                                                                                                                                                                              <  2:433702/273‑1 (MQ=255)                                                                                   gAACCTCTGCCGGGAGAAGACGACGATACGCTTCCGGTTCCCGTCTCCTTCCAAGTGCGTGTACCAGGCAAAAGCGGTGATGAGACTATCGCCCTACGTCAGCGGTAGCACCGTAATCCCCGTCTTTTCCTCGCTTTTTCCCACACGAGTCGCCCGACAATTAACCGGTTGTCAGTAGCGACAGTGGGAGGCCGCAAAGCATAACTGATGACGCAAGAAGTCAGAAAAGCAAGCGATCGCGTTGCATCCAGATACGGCTATTCATTACTCgcgca                                                                                                                                                                                             >  4:308093/1‑274 (MQ=255)                                                                                            gCGGGGGAAGACGACGTTAGGCCTCCGTTTCCAGTCTCTTTACAAGGGCGTGTAACGGGAAACAGCCGCGTTGATAATTTCGCCTAACGTCAGAGGTAGAACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGAAACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAgcg                                                                                                                                                                                <  1:187216/279‑1 (MQ=255)                                                                                            gCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAgc                                                                                                                                                                                 >  1:83408/1‑278 (MQ=255)                                                                                            gCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAGCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAgcg                                                                                                                                                                                <  1:165404/279‑1 (MQ=255)                                                                                                       taacggacacctcccGTTTCCCGTCTCTTTCAAAGGCGGTGTAACAGGCAACTGCGGCTTTGATATTTTCTCCTAACGTCAGAGGTCGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTa                                                                                                                                                                         <  4:501806/262‑1 (MQ=255)                                                                                                                 gtataatagacagTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCTTTGATATTTTCGCCTAACGTCAGAGGTAGAACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCAt                                                                                                                                                            <  1:136166/266‑1 (MQ=255)                                                                                                                       gTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCAtt                                                                                                                                                           >  2:304645/1‑273 (MQ=255)                                                                                                                            cGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAg                                                                                                                                                                             >  2:501806/1‑250 (MQ=255)                                                                                                                                  tttACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGAGCGCTTTGGATCCCGATTCTGGTATTCATTACCCCCGCGGCCTGTGCCAGGCCGGCGTACAGCGCCTGGCCTTccccccacgtttc                                                                                                                                              >  4:299491/1‑263 (MQ=255)                                                                                                                                          ggCGTTTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGACGCATAATTAGCCGATAACAAGTAGCGACACCTGAAGACCGAAACACCTCACTGATTCCGCGATAAGCAAGACAAGCAATCTCTCGGTTTGGATCCCGATTCCTGTATTCATTACCCTCGCGGACTGTTCCACCGCAGGTTAAAGCTATTAGCATTCGCATCCGGTTGCCGCTgcc                                                                                                                                       >  4:216735/1‑272 (MQ=255)                                                                                                                                            cGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCCGGTTACCGCTAATGGCATTCGCATCCGGTTTCCCCTgacga                                                                                                                                     >  4:454804/1‑274 (MQ=255)                                                                                                                                            cGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTgacga                                                                                                                                     >  2:47943/1‑274 (MQ=255)                                                                                                                                              tgtAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTg                                                                                                                                                                                                                                                                 >  4:475648/1‑148 (MQ=255)                                                                                                                                              tgtAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCGGACGACAAAgc                                                                                                                               >  1:151384/1‑278 (MQ=255)                                                                                                                                              tgcaaCAGGCAACAGCGGCGTTGATATTTTCGCCTAACGCCAGAGGTAGCACCGTAACTCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGTTAATTAGCCGCTAAGCAGTAGCGACACAGGAAGACCGCAACACATGACCGATTACGCGTTAAGCAAGAAACGCAAGCAATCCCTTGTGATCCCGAGCCTGGTATTCATTACCCCCGCGGCCTGTGCCAGCCCAGGGTACACCTTCTGGCTTTCGTATCACGTTCTCGCTtacgac                                                                                                                                    >  4:184827/4‑273 (MQ=255)                                                                                                                                                  aCAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTa                                                                                                                                                                                                                                                             <  2:475648/148‑1 (MQ=255)                                                                                                                                                             cggcgTTGATATTTTCGCCTAACGTCCGAGGTAGCACCGTAATCCGCGTCTTTTACCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGAACGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGACGGTTACCACTACTGGCATTCGCATCCGGTTGCCGCGGAAGACAAAGCCAAGNaaaccgc                                                                                                                   >  4:562017/1‑270 (MQ=255)                                                                                                                                                                                 aaCGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGtcttct                                                                                               <  2:209260/275‑1 (MQ=255)                                                                                                                                                                                      cAGAGGATGAACAGTAACCCGCGGCTTTTCCCCGCTTTGTTGCGCTAAAGAAGCAGGAAAATTAGCGGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGGGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTAcgcg                                                                                          <  4:304645/275‑1 (MQ=255)                                                                                                                                                                                            tAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCt                                                                               >  1:61898/1‑279 (MQ=255)                                                                                                                                                                                              gCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTg                                                                                                                                                                                                               >  1:34702/1‑150 (MQ=255)                                                                                                                                                                                              gCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTg                                                                                                                                                                                                               <  3:34702/150‑1 (MQ=255)                                                                                                                                                                                                    tAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCTGATAAGCAGTAGCGACACAGGAAGACGGCAACACATCACTGATTACTCGATAAGCCCGAAAAGCAAGCTATAGCCGTCGGACGCGATTCTGTTATTCATTACCCCCGCGGCCTGTCCCAGCTCAGGTTACAGCTACTCGCACTCGCTTCTGGTTGCCGCTGACGACACAGCCCAGCAACGCGTCGTTCACCCACGGTTCTACTACCCT‑CTGGACACCtacgc                                                                           >  4:486314/1‑271 (MQ=255)                                                                                                                                                                                                     aaTCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCCTGCCCTCACCGGGCCTCCACGCG‑CTGGATAcccttgcc                                                                          >  4:201254/1‑269 (MQ=255)                                                                                                                                                                                                         cgcgTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTAcg                                                                                            <  2:201254/254‑1 (MQ=255)                                                                                                                                                                                                         cgcgTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCAAGCGCAGGTTACAGCTAATGGCATTGGCATCAGGATGCCGCTGACCACAACGGGCAGCAACGCGTCGCGCACCCACCGGTCTTATACCCC‑CGGGATACCttacaggtac                                                                      >  4:530546/1‑267 (MQ=255)                                                                                                                                                                                                           cgTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAaagcaag                                                                                                                                                                                                                                       >  2:111322/1‑113 (MQ=255)                                                                                                                                                                                                            gTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTa                                                                                                                                                                                                     <  1:32977/146‑1 (MQ=255)                                                                                                                                                                                                            gTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTa                                                                                                                                                                                                     >  3:32977/1‑146 (MQ=255)                                                                                                                                                                                                            gTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCCCGGCCTGAGCCAGCGCAGGTTACAGCTACCGGCATTACCATCAGGTTGCCGCTGCCGGCAAAGCGCCGCAACGCATGGTGCCCCCACCGGTCTTCCACCAG‑CTGGCTACCTTCACGGCCAAtt                                                                   >  4:357802/1‑275 (MQ=255)                                                                                                                                                                                                               ttttCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGAt                                                                                                                                                                                                                                   <  4:111322/113‑1 (MQ=255)                                                                                                                                                                                                                 ttCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTg                                                                                                                                                                >  4:212695/1‑178 (MQ=255)                                                                                                                                                                                                                     ccGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCAt                                                                                                                                                            <  2:212695/178‑1 (MQ=255)                                                                                                                                                                                                                        cTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGAACCCGATTCTGGCATTCATTACCCTCGCGGCCTGGGCCAGCGCAGGTTACAGCTACTGGCATTCGCGACAGGGTGCACCGGACGGAAAACCGCACGCACGCCGCGTTCCCCCCCCGGTCTTC‑ACCCGCCTTAATCACTTCACGGTCAATTTGGGCGAtaccg                                                       >  4:379185/1‑271 (MQ=255)                                                                                                                                                                                                                         tnttttgAGCACAAAACTCAGAAAAAATAGGCANNAAGCAGCAAGAACACAGGAAGACCCAAACANATGACTGATTACGCGATAAGAAAAAAAAGCAACCGATCTCTTTGGATCCCGATTCTGGTATTCATTACCCTCGGNGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCATGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATcgc                                                  <  3:67899/275‑1 (MQ=255)                                                                                                                                                                                                                          ttgttgCGCTCAAAACGCAGGATAATTAGCCGATAAGCAGTGGCGAAACAGGAAGACCGCAACACATGACTGATTACCCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATcgcg                                                 <  1:106238/279‑1 (MQ=255)                                                                                                                                                                                                                              tgCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAATACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATcgcg                                                 <  2:562017/275‑1 (MQ=255)                                                                                                                                                                                                                              tgCGCTCAAGACGAAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATcgcg                                                 <  2:5912/275‑1 (MQ=255)                                                                                                                                                                                                                                       gACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACAGTACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATCGCGTACTTtata                                        <  2:66643/275‑1 (MQ=255)                                                                                                                                                                                                                                        aCGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATCGCGTACTTTatat                                       <  2:454804/275‑1 (MQ=255)                                                                                                                                                                                                                                            aGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGC‑ACACATGACTGATTACGCGATAAGCAAGACAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTTGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTACCCGGTCTTCTACGCG‑CTGGGTACCtt                                                                              >  4:353184/1‑231 (MQ=255)                                                                                                                                                                                                                                            aGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGC‑ACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCtt                                                                              >  4:340829/1‑231 (MQ=255)                                                                                                                                                                                                                                                tAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGC‑ACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACg                                                                          <  2:353184/231‑1 (MQ=255)                                                                                                                                                                                                                                                tAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGC‑ACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACg                                                                          <  2:340829/231‑1 (MQ=255)                                                                                                                                                                                                                                                 aaTTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGGATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATCGCGTACTTTATATCGGCATAACCCTg                          <  1:104272/279‑1 (MQ=255)                                                                                                                                                                                                                                                        cGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGGCAATTTGGGCGATGCGGATCGCGTACTTTATATCGGCATAACCCTgc                         >  2:72962/1‑273 (MQ=255)                                                                                                                                                                                                                                                                           aGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTAACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATCGCGTACTTTATATCGGCATAACCCTGCGCCTGAAAGATGAAGCTAc      <  2:547507/273‑1 (MQ=255)                                                                                                                                                                                                                                                                             gAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACACCTACTGGCATTCGCATCCGGTTGCCGCTGACGCCAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCCCGGTCAATTTGGGCGATCCGGATGCCGTTGTTTATATCGGCATACCCCTGCGCCGAAAAAATGAAGCCACCCGc  >  4:332865/1‑275 (MQ=255)                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                           AAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGTCGTGCCCTCACCGGTCTTCTACGCG‑CTGGATACCTTCACGGTCAATTTGGGCGATGCGGATCGCGTACTTTATATCGGCATAACCCTGCGCCTGAAAGATGAAGCTACCCGC  >  NC_000913/2019336‑2019878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑