Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,993,145 C→A 100% R665S (CGT→AGT)  cyaA → adenylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,993,1450CA100.0% 153.7 / ‑6.7 49R665S (CGT→AGT) cyaAadenylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (23/23);  total (25/24)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAA  >  NC_000913/3992874‑3993410
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                         
tttCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTTGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCATACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGt                                                                                                                                                                                                                                                                         <  4:406778/274‑1 (MQ=255)
     gTAAGCTGGATTTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCa                                                                                                                                                                                                                                                               <  1:15885/279‑1 (MQ=255)
           tGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCGGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACGTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCAcc                                                                                                                                                                                                                                                             <  2:543771/275‑1 (MQ=255)
                                       aaaTTGCCTGGGAGGTAGCGTGGACCTGCTGTACGGCAACCCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGgcg                                                                                                                                                                                                                                  <  4:447738/274‑1 (MQ=255)
                                                   gnttggCGTTTCCCTGCGGTACCCCAACTCATGNNACGAAGCGCCTACGCTGCTCTTCAACCGCGNGCAATCGATGATCGAAGCCCTGAAACCTAGTCTCGGCAAAAAGCATCAGGACGGCGCACCGCCAGATAGCGTGGNAGTCTTCTGTTATGGCCAGCAGCTGCGCAGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCa                                                                                                                                                                                                                 <  3:74294/274‑1 (MQ=255)
                                                     gtggtgtgtgccTGGTGGACGGAAACTCGTGGAACGAAGTGCGTACGCGGGAATTAAACGGCGAGAAATCGAGGATCGAAGCCCTGAAACCTAGTTTCGGCAAAATGCATCGGGACGCCGCACCGCCAGATAGCGTTGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACGGGTTTCTGAGTGTATTGAATTGGGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGt                                                                                                                                                                                                                   <  4:107087/265‑1 (MQ=255)
                                                     gtagCGTTGACCTGCGGTACCGCAACTCGGTGAACGAAGTGCGTACGCTGCACTTCAAAGGCGAGCAATGGATGATCGAAGGCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGGTAGCGTGGAAGTCTTCTGTTATAGCCAGCCTCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGt                                                                                                                                                                                                                   <  4:251623/275‑1 (MQ=255)
                                                     gtagCGTGTACCTGCTGTACCGCCACGCGTGGAAAGAAGTGCGTACCCTGCACTTCAAGGGGGAGTAACGGAAGGTCGAAACCCTGAAACCAAATCTCGGCAAAAGTCAGCAGGAGGCCGTACGGCCAGGTAGCGGGGAAGTATGCTGTGCTAGCAAGCATCTGGGCGGCTTAATTCGTACTCGCGTGCAGGAACTGGTTTCTGGGGGTATTGAATGGAGTCTTTCCAGCACCCACCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGt                                                                                                                                                                                                                   <  4:118997/275‑1 (MQ=255)
                                                           ttcaCCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGCAACCGGGCGTTTCAAGGCCCTGCGCGTTTCTGGTCAAAc                                                                                                                                                                                                              >  2:516833/4‑274 (MQ=255)
                                                               ccTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCGGCGCGTTCCTGGGCAAACCTggg                                                                                                                                                                                                         >  4:575848/1‑275 (MQ=255)
                                                                   ctgTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGt                                                                                                                                                                                                                   <  2:575848/261‑1 (MQ=255)
                                                                   ctgTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTACTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGgt                                                                                                                                                                                                      <  2:515383/274‑1 (MQ=255)
                                                                           cAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGCTAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATGGCATTGAGTCTTTCCAGCACCCGCCAGGACACCGGGCGTTTCAAGGCGCTGCGCGTTCCTGGTCAAACCTGGGGGTTGttcttag                                                                                                                                                                                             >  4:247889/1‑273 (MQ=255)
                                                                           cAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCg                                                                                                                                                                                             >  2:11486/1‑275 (MQ=255)
                                                                             aCTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGGTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGaa                                                                                                                                                                                           >  4:306721/1‑275 (MQ=255)
                                                                              cTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCt                                                                                                                                                                                      >  1:67934/1‑279 (MQ=255)
                                                                                 gTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGGTCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGc                                                                                                                                                                                        <  2:379779/274‑1 (MQ=255)
                                                                                 gTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGaa                                                                                                                                                                                   <  1:47302/279‑1 (MQ=255)
                                                                                 gTGGAACGAAGTGCGTACGCTGCACTTCAACGGAGAGCAATCGATGATCGAAGCCCGGAAAACTATTCTCGGCAAAATGCATCAGGACGACGCACCGCCAAATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGAGGTTGTTCTTCGAACGCCTGaa                                                                                                                                                                                   <  1:110328/279‑1 (MQ=255)
                                                                                   ggAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAACGCATCAGGACGCCGCACCGCCAGATAGCGTGGACGTCTGCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTAGCTTTCAGCAACTGGTTTCTGAGTGTATTGAATCGCGTCTTTCCAGCCCCTGCCCGGAAACCGGGAGTTCCCAGGCGCTGCGCCTTTCGGGTCAAACCTGGGGGTTGTTCTTCGacccgcct                                                                                                                                                                                     >  4:320180/1‑268 (MQ=255)
                                                                                                            cAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAAt                                                                                                                                                                                  >  4:148301/1‑253 (MQ=255)
                                                                                                               cGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGACCGCCTGAATGAATCGGTACAGAAACTGGAAAACGcaaa                                                                                                                                                     >  1:13598/1‑276 (MQ=255)
                                                                                                                ggCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTAt                                                                                                                                                                              <  2:148301/253‑1 (MQ=255)
                                                                                                                      agaTCGATGGTCGAAGCCCCGAAAACTATTCACGGCAAAATGGCTCCGGAGGCCGAACCGCCGGAATGCGTTGTAGTCTTCTGGTATAGCCAGCATCTGAGCGGCTTAACTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATGGAGTATTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTGCTGGTCAAACCTGGGGGTTGTTCTTCGAACGTCTGAATGTATCGGTACAGAAACTGGAAAACGCCATc                                                                                                                                                    <  4:48090/271‑1 (MQ=255)
                                                                                                                                   aaGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATg                                                                                                                                                                                 >  4:56043/1‑231 (MQ=255)
                                                                                                                                   aaGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGc                                                                                                                                                        >  2:48090/1‑256 (MQ=255)
                                                                                                                                       ccTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATc                                                                                                                                                                             <  2:56043/231‑1 (MQ=255)
                                                                                                                                                               gctgcAGAACGCCGCACGGCCAGATTGCGTGGAAGTCTTCTGTTATGTCCAGCATCTGCGCGGCTTAATTCGTACGCGCGTGCAGCAACTGGTTTCGGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGt                                                                                                         <  4:444722/271‑1 (MQ=255)
                                                                                                                                                                    aGGACGCCGAACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTg                                                                                                    <  2:399233/275‑1 (MQ=255)
                                                                                                                                                                          ccgcACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGttct                                                                                                                                                                                                >  4:610621/1‑177 (MQ=255)
                                                                                                                                                                           cgcACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGAGTTTCAAGGACCTGCCCGTTTCTGTTCATACCTGGGGGTTGTTCTTCGAACCCCGGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTAGCATAACAAACTGCACGACCTGtattgacaggtg                                                                                              >  4:280002/1‑263 (MQ=255)
                                                                                                                                                                              aCCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGa                                                                                                                                                                                            <  2:610621/177‑1 (MQ=255)
                                                                                                                                                                                                    ttctGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATc                                                                                                                                                                             <  3:29099/170‑1 (MQ=255)
                                                                                                                                                                                                    ttctGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATc                                                                                                                                                                             >  1:29099/1‑170 (MQ=255)
                                                                                                                                                                                                    ttctGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACcggc                                                                    >  2:377629/1‑275 (MQ=255)
                                                                                                                                                                                                              gCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGTTTGAAACCAATCCCTTCCAATTACCGGCGGTGGTTGAc                                                          >  2:104080/1‑275 (MQ=255)
                                                                                                                                                                                                                   cATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGCGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACAGTGCGTTTCAAGGCGCTTCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTGATGTCGCGTTTTGGCATAATAAACTTCCCGGCCTTTCAGGTACGGTTGCAACTCATCACGTCACATTACCGGCGGTTGTGCACggat                                                      >  4:345160/1‑272 (MQ=255)
                                                                                                                                                                                                                   cATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAACCCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGTAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCACGTTGAAACCAATCACGTCAAATTACCGGCGGGGTGGGACGGCtt                                                     >  2:508096/1‑275 (MQ=255)
                                                                                                                                                                                                                   cATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAACCTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCCGTCCAGGTTGAAACCAATCCCGTCAAATTACCGGCGGTGGTGGAAGGCtt                                                     >  4:45155/1‑275 (MQ=255)
                                                                                                                                                                                                                         cgcgGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGGGGACGGCCTTGCCa                                                >  2:6120/1‑274 (MQ=255)
                                                                                                                                                                                                                           gggCTTAATTCGTACTCGCGAGCAGCAACTGGTTTCTGAGTGTATTTAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCg                                             <  2:544384/274‑1 (MQ=255)
                                                                                                                                                                                                                              cTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCAAGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGAGGGCTTTGCCAGCGa                                            >  2:561190/1‑273 (MQ=255)
                                                                                                                                                                                                                                        aCTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATAGCCTCTTTCCAGCACACGCCAGGAAACAGGGCGTTTCAAGTCGCTGCGCGCTTTTGGTCAAAACTGGGGGTCGTTCTTCGAACGCCTGAATGTATCGGTCAAGAAACTGGAAAACGCCATCTAGGTTTATGTCTCATATTCGTCTAACACACAGCACGACCTGTCAGTTACGGTTGAAACCAACTCCCTCACATTAACGTGGCGCGTGTACGGCTTTGCCCGCGCAgggccaaccca                                >  4:225806/1‑267 (MQ=255)
                                                                                                                                                                                                                                             cgTGCAGCAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCCCGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCtttt                        >  1:179911/1‑278 (MQ=255)
                                                                                                                                                                                                                                                         ggTTTCTGAGTGTATTGAATGGAGGCTTTCCAGCACCTGCCACGAAACCGGGCGATTCAAGGCGCTGCGCTTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAGCTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAAGCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGaaa               <  2:302362/275‑1 (MQ=255)
                                                                                                                                                                                                                                                            ttCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGACGGGATCATCCAGGTCTTTTTCgaagaacc              >  2:167411/1‑271 (MQ=255)
                                                                                                                                                                                                                                                                 aGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGaaaa                                                                                                                                                           >  3:61591/1‑127 (MQ=255)
                                                                                                                                                                                                                                                                 aGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGaaaa                                                                                                                                                           <  1:61591/127‑1 (MQ=255)
                                                                                                                                                                                                                                                                  gtgtAGTGAAGTTAGTCTTTCCCGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGaa  <  1:103353/279‑1 (MQ=255)
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TTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAGCAACTGGTTTCTGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAA  >  NC_000913/3992874‑3993410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: