Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,117,047 G→T 100% S59Y (TCC→TAC)  glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,117,0470GT100.0% 211.6 / ‑6.7 66S59Y (TCC→TAC) glpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (31/33);  total (32/34)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAT  >  NC_000913/4116783‑4117318
                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                
tCGAGGATCCACTTCACTTTTGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTCAAGATAGGATTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCCATTTGGGCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCCGCGGTTAGCTTTggg                                                                                                                                                                                                                                                                         >  4:49119/1‑274 (MQ=255)
    ggATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGcc                                                                                                                                                                                                                                                                    <  2:326469/275‑1 (MQ=255)
          acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGAtt                                                                                                                                                                                                                                                              >  2:273815/1‑275 (MQ=255)
          acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGAtt                                                                                                                                                                                                                                                              >  2:91360/1‑275 (MQ=255)
                 tttGGTCGCAGAAAATGACGGTGCAATCACCCGACCGTTATTGCTGCGGATACAATCTTCGAAACCGTCACGTTTTAAATGCGCGCAGATTTCTGCGGTACTACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAACAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                                                                                   <  1:23866/279‑1 (MQ=255)
                 tgtggtggCAGTAAAAAAAGGGTCAATCACCAGACCGGGATTGCTGTGGTTGTAATCTTCTAAACCGTCGCGTTTTAAATGCTCGCAGATTTCTTCGGTACGACGGCACTGCCAGACAATGGCGTTATAGTTAGGCTTGCCGGTTTCTTTTTCCCAGATAATGGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCTGCGTGTAGCTTTGGGTGGCCCAGATTTCCAtt                                                                                                                                                                                                                                                        <  4:291017/272‑1 (MQ=255)
                          aGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATATCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCTCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGTCCCAGGTTTCCTTTGGGTTGTGTTCtcc                                                                                                                                                                                                                                         >  1:47060/1‑277 (MQ=255)
                               ggTACGGGTCAATCACCAGAACGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAc                                                                                                                                                                                                                                    <  1:146122/278‑1 (MQ=255)
                                           tCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CCATTCTACCAGCGTGGAGCTTTGGGTGGCACAGTTTTCCATTGGGTCGTTTTCTAccacaccgtgccc                                                                                                                                                                                                                             >  4:88995/1‑263 (MQ=255)
                                           tCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTNTAGCCCG‑‑CACTTCTACCAGCGTGTAGCCTTTGGTGGCCCAGTTTTCCATTGGTTCGTTTTTGACCCAATCTGGTTTTgtgg                                                                                                                                                                                                                        >  3:146122/1‑276 (MQ=255)
                                              ctaGACCGGTATTGCTGCGGATATAATCTTCGAAACCGTGACGTTTTAAATGGTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGTCAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGTTCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTggg                                                                                                                                                                                                                         <  4:504320/273‑1 (MQ=255)
                                               cAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtg                                                                                                                                                                                                                                                <  2:88995/252‑1 (MQ=255)
                                                  aCCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAg                                                                                                                                                                                                                      >  2:556977/1‑274 (MQ=255)
                                                      gTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTCCCAGCGTGTAGCTTTTGGTGTCCCAGATTTACATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGc                                                                                                                                                                                                                >  1:43238/1‑276 (MQ=255)
                                                       tATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGACGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCCG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTg                                                                                                                                                                                                                 >  2:504320/1‑274 (MQ=255)
                                                        aTTGCTGCGGATATAATCTTCTAAACCGTCACGGTTTAAATGCTCGCAGATTTCTGCCGTACGTCGGCACTGCCAGACAATGGCGTTCTAGATAGGCTTGCCGGTTTCTTTTTCCCCGACAATAGTGGTTTCACGCTGGTTCGTAATAACGATAGCTGCAATTTGGTCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCt                                                                                                                                                                                                               <  2:179876/275‑1 (MQ=255)
                                                            ctgcGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTCTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGGTTTGGGTAGATTTGCTCaa                                                                                                                                                                                                            >  2:570323/1‑274 (MQ=255)
                                                              gcGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGAAAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgcg                                                                                                                                                                                                     <  1:44454/279‑1 (MQ=255)
                                                                 gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGGTTCTTTCTGCCAGCCAATAGTGGTTTCACGCCGGTTAGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTAACAGCGTGTAGCTACGGGTGGCCCGGGATTCCATTGGGTCGTTTTCCTACACACCTGGGTTTGTGTAGGTTAGCTCGAATTcac                                                                                                                                                                                                      >  4:94850/1‑273 (MQ=255)
                                                                  atatAGTGTTCTAAGCCTGCACGTTATAAATTCTAGGAGGTTTCGTCGGTACGGCGGCTCTGCCCGACTATGGCGTTATCGATAGGCTTGCCGGTTTCTGTTGCCCAAAACAGAGTGGGTTCGCGCTGCTTCGTCATACCGATAGCTGCAATTTGATCGGAACTGCGATCGGCGTTCGCCAG‑‑CACTTCTACCTGCGTGTAGCTTTGAGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGGTTTGGGTAGATTTGCTCAAATTcgcg                                                                                                                                                                                                     <  4:213065/275‑1 (MQ=255)
                                                                           tctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTc                                                                                                                                                                                                                                            >  4:574827/1‑227 (MQ=255)
                                                                           tctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                                                                             >  2:343870/1‑274 (MQ=255)
                                                                               aaCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAcc                                                                                                                                                                                                                                        <  2:574827/227‑1 (MQ=255)
                                                                                aCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                                                                        <  4:91360/274‑1 (MQ=255)
                                                                                    tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCTCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTTGCCCAGATTTCCATTGGGTCGTGTTTTCCCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCTCGCTGCTACACGCTAATTat                                                                                                                                                                                   >  4:444066/1‑275 (MQ=255)
                                                                                    tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTTGCCCAGATTTCCATTGGGTCGTGTTc                                                                                                                                                                                                                                            >  2:250730/1‑218 (MQ=255)
                                                                                    tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGacc                                                                                                                                                                                             >  1:3078/1‑264 (MQ=255)
                                                                                      aCGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTc                                                                                                                                                                                                                                                            >  4:358121/1‑201 (MQ=255)
                                                                                        gTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTggg                                                                                                                                                                                                                                                     <  2:195212/206‑1 (MQ=255)
                                                                                          tttAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCAtt                                                                                                                                                                                                                                                        <  2:358121/201‑1 (MQ=255)
                                                                                              aaTGCTTGCAGATTTCTTGGGTACGACGGCACTGCCAGACAATGGCGTTATGGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTCCCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCg                                                                                                                                                                         <  4:343870/275‑1 (MQ=255)
                                                                                                 gCTCGCAGATTTCTGCGGTACGACGGCACGGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                                      <  2:49119/275‑1 (MQ=255)
                                                                                                   tCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCNTGTTCTACCCAACCTGGTTTTGGGTAGCTTTGCTCACATTCGCGCTGCGCCACGTCAATGCTGTTGGCATtgtgtttta                                                                                                                                                                  >  3:132953/1‑272 (MQ=255)
                                                                                                    cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGCCAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTg                                                                                                                                                                                                                                                       >  2:569682/1‑192 (MQ=255)
                                                                                                        gATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                                                                               <  2:36935/243‑1 (MQ=255)
                                                                                                                 ggTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcg                                                                                                                                                       <  2:444066/274‑1 (MQ=255)
                                                                                                                       ccgGCATTGCCAGACAATGGCGTTAAAGGTAGTCTTGCCGGTTTCTTTTTCCCAGACAATGGGGGTTTCCCGCTGGTTCGTAATCCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTTCCAGCGTGTAGCTTTAGGTGGCCCAGAGTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                                                                                                <  4:273815/274‑1 (MQ=255)
                                                                                                                                                     agGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CCCTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTCCCCAACCTGGTTTTGGGTAGATTTGCCCAAATTCGCGCTTCGCCACGCTAATGATATTGGCCTCGTGATCCATTACGACCGCCCGGAAGCTGGTGGGACCCTGGTCggagccaacgaaa                                                                                                                   >  4:13959/1‑262 (MQ=255)
                                                                                                                                                       gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTTACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGata                                                                                                                   <  2:454008/274‑1 (MQ=255)
                                                                                                                                                       gCTTGCCGGGTTCTTTTTCCCAGACACTACTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGCTCGGAACTGATATCTGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTTGGTGGCCCAGATTTCCATCGGGTCGTGTTCTACCCAACCTGGtt                                                                                                                                                                                                                              <  1:105908/165‑1 (MQ=255)
                                                                                                                                                          tGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGGTTTGGGTAGATTTGCTCAAATTCGCGCTTGGACACGCTCATGGTATTGGCATCGTGCTCCATTACGACCGCGAGGGGGCTTGTGGTGCCCTGGTCGGGCGCAACTGTATAtttt                                                                                                             >  4:284781/1‑275 (MQ=255)
                                                                                                                                                           gCCGGTTTCTTTTTCCCAGACACTAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCCATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCATCCCTGCTGTTGGGTAGATTTTCTCAAATTCGAGCGGCGGCCCGCTAATGATATTGGCTTCGAGCACCCTTACGCCCGCGCTGGGCCTGGTGGTGCCCTGGGCACGCGCAACGATCCAtttt                                                                                                             >  4:37593/1‑274 (MQ=255)
                                                                                                                                                                 gtCTTTTTCCCCGCAAATATGTTTTTCATGCTGGTTCGTAAGACCGCTAGCTGCAATTTGTGCGGACCTGGTATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTTGGTGTCCCAGAGTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAg                                                                                                      <  4:382181/274‑1 (MQ=255)
                                                                                                                                                                                aaTGGTGGTTTCAGCTTGTTTGTGAATGCCGGTNNNTGAGTTGTGAGCGGAAGTGTTAGCGGCTGTCGACAG‑‑CACTTATCCGAGCGGGTGGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATGTGCGAAAATTCGCGCTGCGACACGCGAATGAGATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                                                                   <  3:174609/279‑1 (MQ=255)
                                                                                                                                                                                    gtgttgtCACGCTGTTTCGTATGACCGATAGCTTCAATTGGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTCCCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGTGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                                                                   <  4:378130/269‑1 (MQ=255)
                                                                                                                                                                                    gtgtgttCAAGCTTGTTCTTGATATCGATAGGGTCAATGTTATCGGAACGGATATCGGCTTTTGCCAG‑‑GACTTCTACCAGCGTGTAGGTTTGGTGGGCCCAGATTTGCATTGGGTCGTGGTCTACCCAACCTGGTTTTGGGTAGATGTGCTCAAAATCGGGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTGTTCAGTCATGTTTAATTGTCCCGt                                                                                   <  4:13103/270‑1 (MQ=255)
                                                                                                                                                                                        tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAgctgttt                                                                           >  1:112109/1‑273 (MQ=255)
                                                                                                                                                                                          tCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCat                                                                             >  2:507481/1‑275 (MQ=255)
                                                                                                                                                                                          tCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTAACAGCGTGTAGCTTTGGGTGGCCCAGATTTCCTTTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTCGCTCAAATTCGCGCTGCGACACGCTAAGGATATAGGCATCGTGGTCCATTACGACTGTGAGGGCGCTGGTGGCGCCCTGGTCGTGCGCAACGATATTGTTGTTTGCATGCATGTTTAATTGTCCCGTAGTctt                                                                             >  4:560147/1‑273 (MQ=255)
                                                                                                                                                                                            aCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGat                                                                                                                                                                                   >  4:79129/1‑171 (MQ=255)
                                                                                                                                                                                                tGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTg                                                                                                                                                                               <  2:79129/171‑1 (MQ=255)
                                                                                                                                                                                                    tcctAATACCGATAGCTGCAATTTGATCGGTACTGTTATCGGCTTTCGCCAG‑‑CACTTCTTCCAGCTTGTAGCTTTGGTTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGc                                                                     <  2:569470/270‑1 (MQ=255)
                                                                                                                                                                                                           tACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCGGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCttt                                                             >  2:277572/1‑275 (MQ=255)
                                                                                                                                                                                                             ccGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGCTTTGGGTAGATTTTCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCCACGATATATTTTTTTTCCGTCCTGTTTAATTGTCCCGTAGTCATATTACAGCGACGtttttt                                                           >  2:588232/1‑275 (MQ=255)
                                                                                                                                                                                                                aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGCGCGCAACGATATCTTTTTTTTCAGTCATTTTTAATTGTCCCTTTGTCCTTTTAAAGCACAGCTTTTTGtt                                                        >  4:436147/1‑275 (MQ=255)
                                                                                                                                                                                                                    cTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGa                                                    <  2:284781/275‑1 (MQ=255)
                                                                                                                                                                                                                    cTGCAATTTGAACGGAACTGTATTCGGCTTTCGCCAG‑‑CACTTTTACCAGCTTGTAGGTTTGGGTGGCCCCGATTTCCATTGGGTCGTCTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATCTTGGCAGCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGCCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTATGa                                                    <  4:359682/275‑1 (MQ=255)
                                                                                                                                                                                                                            tGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCACTCCTGTTTAATTTTCCCGTATTCTTATTACAGCCATCCTTTTTGTTCTGAATGAGTtg                                            >  4:248347/1‑275 (MQ=255)
                                                                                                                                                                                                                                            cGGTTTTTGCCGG‑‑CCCTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTttcttc                            <  4:277572/275‑1 (MQ=255)
                                                                                                                                                                                                                                            cGGCTTTGGCAAA‑‑CAGTTTTAACAGGGTTGAGGTTTGGGTGGCCCAGAGTCCCGTTGGGTAGTGTTCTACCCAACCGGGTTTTGGGTAGATTTGCTCAAACTCGCGGTGCGGAACGCGAATGATATGGGCATGGTGGTACAGTAGGACCGGGCGGGGGCGGGTGGTGCGCTGGTCGAGAGCAACGATATATTGTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTttcttc                            <  4:114579/275‑1 (MQ=255)
                                                                                                                                                                                                                                             ggCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTcc                           <  2:436147/275‑1 (MQ=255)
                                                                                                                                                                                                                                              gCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCca                          <  2:248347/275‑1 (MQ=255)
                                                                                                                                                                                                                                                  tCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTACTGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTTTCCCGCAGTCATATTACATCAACGCTTTTTGTTTTGAAGTAGTTTTGGTTTCCTTTTCTTCCACAAAACAg                  >  1:114897/1‑279 (MQ=255)
                                                                                                                                                                                                                                                  tCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATGGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAGATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg                                                                                                                                           <  1:64405/158‑1 (MQ=255)
                                                                                                                                                                                                                                                       aG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGa                 <  2:281038/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                      gCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCCTGTTTAATTGTCCCTGAGTCATTTTACAGCCAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAAATTTctaacagcaa    >  4:568338/1‑266 (MQ=255)
                                                                                                                                                                                                                                                                        gtgtAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTc                                                                                                        >  2:252680/1‑173 (MQ=255)
                                                                                                                                                                                                                                                                        gtgtAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                                                                                            <  3:30101/185‑1 (MQ=255)
                                                                                                                                                                                                                                                                        gtgtAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                                                                                            >  1:30101/1‑185 (MQ=255)
                                                                                                                                                                                                                                                                        gtgtAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAt  >  2:89030/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                
TCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAT  >  NC_000913/4116783‑4117318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: