Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,117,047 | G→T | 100% | S59Y (TCC→TAC) | glpK ← | glycerol kinase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,117,047 | 0 | G | T | 100.0% | 211.6 / ‑6.7 | 66 | S59Y (TCC→TAC) | glpK | glycerol kinase |
Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (31/33); total (32/34) | |||||||||||
Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAT > NC_000913/4116783‑4117318 | tCGAGGATCCACTTCACTTTTGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTCAAGATAGGATTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCCATTTGGGCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCCGCGGTTAGCTTTggg > 4:49119/1‑274 (MQ=255) ggATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGcc < 2:326469/275‑1 (MQ=255) acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGAtt > 2:273815/1‑275 (MQ=255) acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGAtt > 2:91360/1‑275 (MQ=255) tttGGTCGCAGAAAATGACGGTGCAATCACCCGACCGTTATTGCTGCGGATACAATCTTCGAAACCGTCACGTTTTAAATGCGCGCAGATTTCTGCGGTACTACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAACAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTc < 1:23866/279‑1 (MQ=255) tgtggtggCAGTAAAAAAAGGGTCAATCACCAGACCGGGATTGCTGTGGTTGTAATCTTCTAAACCGTCGCGTTTTAAATGCTCGCAGATTTCTTCGGTACGACGGCACTGCCAGACAATGGCGTTATAGTTAGGCTTGCCGGTTTCTTTTTCCCAGATAATGGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCTGCGTGTAGCTTTGGGTGGCCCAGATTTCCAtt < 4:291017/272‑1 (MQ=255) aGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATATCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCTCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGTCCCAGGTTTCCTTTGGGTTGTGTT‑Ctcc > 1:47060/1‑277 (MQ=255) ggTACGGGTCAATCACCAGAACGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAAc < 1:146122/278‑1 (MQ=255) tCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CCATTCTACCAGCGTGGAGCTTTGGGTGGCACAGTTTTCCATTGGGTCGTTTT‑CTAccacaccgtgccc > 4:88995/1‑263 (MQ=255) tCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTNTAGCCCG‑‑CACTTCTACCAGCGTGTAGCCTTTGGTGGCCCAGTTTTCCATTGGTTCGTTTT‑TGACCCAATCTGGTTTTgtgg > 3:146122/1‑276 (MQ=255) ctaGACCGGTATTGCTGCGGATATAATCTTCGAAACCGTGACGTTTTAAATGGTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGTCAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGTTCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTggg < 4:504320/273‑1 (MQ=255) cAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtg < 2:88995/252‑1 (MQ=255) aCCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAg > 2:556977/1‑274 (MQ=255) gTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTCCCAGCGTGTAGCTTTTGGTGTCCCAGATTTACATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGc > 1:43238/1‑276 (MQ=255) tATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGACGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCCG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTg > 2:504320/1‑274 (MQ=255) aTTGCTGCGGATATAATCTTCTAAACCGTCACGGTTTAAATGCTCGCAGATTTCTGCCGTACGTCGGCACTGCCAGACAATGGCGTTCTAGATAGGCTTGCCGGTTTCTTTTTCCCCGACAATAGTGGTTTCACGCTGGTTCGT‑AATAACGATAGCTGCAATTTGGTCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCt < 2:179876/275‑1 (MQ=255) ctgcGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTCTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGGTTTGGGTAGATTTGCTCaa > 2:570323/1‑274 (MQ=255) gcGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGAAAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgcg < 1:44454/279‑1 (MQ=255) gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGGTTCTTTCTGCCAGCCAATAGTGGTTTCACGCCGGTTAGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTAACAGCGTGTAGCTACGGGTGGCCCGGGATTCCATTGGGTCGTTTTCCTACAC‑ACCTGGGTTTGTGTAGGTTAGCTCGAATTcac > 4:94850/1‑273 (MQ=255) atatAGTGTTCTAAGCCTGCACGTTATAAATTCTAGGAGGTTTCGTCGGTACGGCGGCTCTGCCCGACTATGGCGTTATCGATAGGCTTGCCGGTTTCTGTTGCCCAAAACAGAGTGGGTTCGCGCTGCTTCGT‑CATACCGATAGCTGCAATTTGATCGGAACTGCGATCGGCGTTCGCCAG‑‑CACTTCTACCTGCGTGTAGCTTTGAGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGGTTTGGGTAGATTTGCTCAAATTcgcg < 4:213065/275‑1 (MQ=255) tctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑c > 4:574827/1‑227 (MQ=255) tctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca > 2:343870/1‑274 (MQ=255) aaCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTAcc < 2:574827/227‑1 (MQ=255) aCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa < 4:91360/274‑1 (MQ=255) tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCTCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTTGCCCAGATTTCCATTGGGTCGTGTT‑TTCCCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCTCGCTGCTACACGCTAATTat > 4:444066/1‑275 (MQ=255) tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTTGCCCAGATTTCCATTGGGTCGTGTT‑c > 2:250730/1‑218 (MQ=255) tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGacc > 1:3078/1‑264 (MQ=255) aCGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTc > 4:358121/1‑201 (MQ=255) gTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTggg < 2:195212/206‑1 (MQ=255) tttAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCAtt < 2:358121/201‑1 (MQ=255) aaTGCTTGCAGATTTCTTGGGTACGACGGCACTGCCAGACAATGGCGTTATGGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTCCCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCg < 4:343870/275‑1 (MQ=255) gCTCGCAGATTTCTGCGGTACGACGGCACGGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa < 2:49119/275‑1 (MQ=255) tCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCNTGTT‑CTACCCAACCTGGTTTTGGGTAGCTTTGCTCACATTCGCGCTGCGCCACGTCAATGCTGTTGGCATtgtgtttta > 3:132953/1‑272 (MQ=255) cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGCCAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTg > 2:569682/1‑192 (MQ=255) gATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa < 2:36935/243‑1 (MQ=255) ggTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcg < 2:444066/274‑1 (MQ=255) ccgGCATTGCCAGACAATGGCGTTAAAGGTAGTCTTGCCGGTTTCTTTTTCCCAGACAATGGGGGTTTCCCGCTGGTTCGT‑AATCCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTTCCAGCGTGTAGCTTTAGGTGGCCCAGAGTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc < 4:273815/274‑1 (MQ=255) agGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CCCTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTCCCCAACCTGGTTTTGGGTAGATTTGCCCAAATTCGCGCTTCGCCACGCTAATGATATTGGCCTCGTGATCCATTACGACCGCCCGGAAGCTGGTGGGACCCTGGTCggagccaacgaaa > 4:13959/1‑262 (MQ=255) gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTTACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGata < 2:454008/274‑1 (MQ=255) gCTTGCCGGGTTCTTTTTCCCAGACACTACTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGCTCGGAACTGATATCTGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTTGGTGGCCCAGATTTCCATCGGGTCGTGTT‑CTACCCAACCTGGtt < 1:105908/165‑1 (MQ=255) tGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGGTTTGGGTAGATTTGCTCAAATTCGCGCTTGGACACGCTCATGGTATTGGCATCGTGCTCCATTACGACCGCGAGGGGGCTTGTGGTGCCCTGGTCGGGCGCAACTGTATAtttt > 4:284781/1‑275 (MQ=255) gCCGGTTTCTTTTTCCCAGACACTAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCCATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCATCCCTGCTGTTGGGTAGATTTTCTCAAATTCGAGCGGCGGCCCGCTAATGATATTGGCTTCGAGCACCCTTACGCCCGCGCTGGGCCTGGTGGTGCCCTGGGCACGCGCAACGATCCAtttt > 4:37593/1‑274 (MQ=255) gtCTTTTTCCCCGCAAATATGTTTTTCATGCTGGTTCGT‑AAGACCGCTAGCTGCAATTTGTGCGGACCTGGTATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTTGGTGTCCCAGAGTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAg < 4:382181/274‑1 (MQ=255) aaTGGTGGTTTCA‑GCTTGTTTGTGAATGCCGGTNNNTGAGTTGTGAGCGGAAGTGTTAGCGGCTGTCGACAG‑‑CACTTATCCGAGCGGGTGGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATGTGCGAAAATTCGCGCTGCGACACGCGAATGAGATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt < 3:174609/279‑1 (MQ=255) gtgttgtCACGCTGTTTCGT‑ATGACCGATAGCTTCAATTGGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTCCCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGTGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt < 4:378130/269‑1 (MQ=255) gtgtgttCAAGCTTGTTCTT‑GATATCGATAGGGTCAATGTTATCGGAACGGATATCGGCTTTTGCCAG‑‑GACTTCTACCAGCGTGTAGGTTTGGTGGGCCCAGATTTGCATTGGGTCGTGGT‑CTACCCAACCTGGTTTTGGGTAGATGTGCTCAAAATCGGGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTGTTCAGTCATGTTTAATTGTCCCGt < 4:13103/270‑1 (MQ=255) tttCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAgctgttt > 1:112109/1‑273 (MQ=255) tCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCat > 2:507481/1‑275 (MQ=255) tCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTAACAGCGTGTAGCTTTGGGTGGCCCAGATTTCCTTTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTCGCTCAAATTCGCGCTGCGACACGCTAAGGATATAGGCATCGTGGTCCATTACGACTGTGAGGGCGCTGGTGGCGCCCTGGTCGTGCGCAACGATATTGTTGTTTGCATGCATGTTTAATTGTCCCGTAGTctt > 4:560147/1‑273 (MQ=255) aCGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGat > 4:79129/1‑171 (MQ=255) tGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTg < 2:79129/171‑1 (MQ=255) tcct‑AATACCGATAGCTGCAATTTGATCGGTACTGTTATCGGCTTTCGCCAG‑‑CACTTCTTCCAGCTTGTAGCTTTGGTTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGc < 2:569470/270‑1 (MQ=255) tACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCGGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCttt > 2:277572/1‑275 (MQ=255) ccGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGCTTTGGGTAGATTTTCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCCACGATATATTTTTTTTCCGTCCTGTTTAATTGTCCCGTAGTCATATTACAGCGACGtttttt > 2:588232/1‑275 (MQ=255) aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGCGCGCAACGATATCTTTTTTTTCAGTCATTTTTAATTGTCCCTTTGTCCTTTTAAAGCACAGCTTTTTGtt > 4:436147/1‑275 (MQ=255) cTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGa < 2:284781/275‑1 (MQ=255) cTGCAATTTGAACGGAACTGTATTCGGCTTTCGCCAG‑‑CACTTTTACCAGCTTGTAGGTTTGGGTGGCCCCGATTTCCATTGGGTCGTCTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATCTTGGCAGCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGCCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTATGa < 4:359682/275‑1 (MQ=255) tGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCACTCCTGTTTAATTTTCCCGTATTCTTATTACAGCCATCCTTTTTGTTCTGAATGAGTtg > 4:248347/1‑275 (MQ=255) cGGTTTTTGCCGG‑‑CCCTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTttcttc < 4:277572/275‑1 (MQ=255) cGGCTTTGGCAAA‑‑CAGTTTTAACAGGGTTGAGGTTTGGGTGGCCCAGAGTCCCGTTGGGTAGTGTT‑CTACCCAACCGGGTTTTGGGTAGATTTGCTCAAACTCGCGGTGCGGAACGCGAATGATATGGGCATGGTGGTACAGTAGGACCGGGCGGGGGCGGGTGGTGCGCTGGTCGAGAGCAACGATATATTGTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTttcttc < 4:114579/275‑1 (MQ=255) ggCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTcc < 2:436147/275‑1 (MQ=255) gCTTTCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCca < 2:248347/275‑1 (MQ=255) tCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTACTGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTTTCCCGCAGTCATATTACATCAACGCTTTTTGTTTTGAAGTAGTTTTGGTTTCCTTTTCTTCCACAAAACAg > 1:114897/1‑279 (MQ=255) tCGCCAG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATGGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAGATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg < 1:64405/158‑1 (MQ=255) aG‑‑CACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGa < 2:281038/275‑1 (MQ=255) gCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCCTGTTTAATTGTCCCTGAGTCATTTTACAGCCAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAAATTTctaacagcaa > 4:568338/1‑266 (MQ=255) gtgtAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTc > 2:252680/1‑173 (MQ=255) gtgtAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa < 3:30101/185‑1 (MQ=255) gtgtAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa > 1:30101/1‑185 (MQ=255) gtgtAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAt > 2:89030/1‑275 (MQ=255) | TCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGT‑AATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑‑CACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTT‑CTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAT > NC_000913/4116783‑4117318 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |