Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,685,527 A→G 100% G354G (GGT→GGC fumC ← fumarate hydratase (fumarase C),aerobic Class II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,685,5270AG100.0% 176.8 / ‑6.7 52G354G (GGT→GGCfumCfumarate hydratase (fumarase C),aerobic Class II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (26/23);  minor base T (2/0);  total (29/23)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCA  >  NC_000913/1685261‑1685786
                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                   
cccAGGGCAAGGGCCGACGCTTTTAAGGTCATCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAgc                                                                                                                                                                                                                                                             <  4:545130/275‑1 (MQ=255)
  caccctaatggCCGCAGCTTTTAAGTTCGGCCCTACTTTATGCGCCTGTTTGGCGATCTCGTCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCATTCACCAGCATCAGCGATTCATTGAGTAATTGATTGGTTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAAACGTGGAGCACCACAGGACGCAAGACGTTCAGTTCAAAGTTGCCGGAAgcgc                                                                                                                                                                                                                                                           <  4:84118/266‑1 (MQ=255)
     cgcAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGcctc                                                                                                                                                                                                                                                        >  2:219398/1‑273 (MQ=255)
                              aGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATCCCCAATGTGGGTGTTAAGTGCAGTCACCCGCATCAGCGATTCATTGAGTAATTGATTGCTTCGCTCAAGATTCGGTTCAATACCCACTGCGCCGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGATATTGTTGAACACCCTTGGCCGGAAGACGTTCAGTTCAAAGTTTCCGGAAGCCCCCCCCATTTTGCCAGCCACGGCGTcccc                                                                                                                                                                                                                               >  4:453541/1‑275 (MQ=255)
                                          tGCGCATTTTTGGCGAGCTCGGCGGTTTTGTCATAACCAATGTGTGTGTTAAGCGCAGGCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGa                                                                                                                                                                                                                     <  2:453541/273‑1 (MQ=255)
                                           gggcTGTTTTGGCGATCTCGGGGGCCTTGTCAAAACCAATGTGGGTTTTAAGCGCAGTAACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTTGCGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                                                                                                                                                  <  4:18580/273‑1 (MQ=255)
                                             tcTTTTTTGGTATTCTCGTCGGCTTTGTCATATNNNTTTTTGGTCTTAATCCCATTCACCTGCATTAGCGATTCATTGAGTAATTTATTGATTCGCTCACGATTCGTTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATTCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGTACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                                                                                                                                            <  3:183298/278‑1 (MQ=255)
                                                 ttttgtgtaTCTCGGCGGTGTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                                                                                                                                            <  2:162680/267‑1 (MQ=255)
                                                 ttttGGCGATCACGGCGACTTTGTCATAACCAATGTGGATGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                                                                                                                                            <  2:443508/275‑1 (MQ=255)
                                                        gATCTCGGCAGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                                                                                                                                     <  2:326587/275‑1 (MQ=255)
                                                        cacttccgcgtcTTTGTCATAACCAATGTGGGTGTTAAGAGAAGTGCCCAGCATCTGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGGGGTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGGACCGATTGCAGGAAATTGGGGATCACCATTGGACGGAAGACGTGCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                                                                                                                                     <  4:147336/268‑1 (MQ=255)
                                                         aTCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAc                                                                                                                                                                                                                    >  4:467019/1‑259 (MQ=255)
                                                             cggcggCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagca                                                                                                                                                                                                                <  2:467019/259‑1 (MQ=255)
                                                                     ttGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACt                                                                                                                                                                                         <  2:111193/274‑1 (MQ=255)
                                                                      tGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATg                                                                                                                                                                                                         >  4:468858/1‑257 (MQ=255)
                                                                          aTAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                                                                                                                                     <  2:468858/257‑1 (MQ=255)
                                                                                  tgtgGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACt                                                                                                                                                                           >  2:144154/1‑275 (MQ=255)
                                                                                         gtTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCAcg                                                                                                                                                                                                                                       >  4:141097/1‑208 (MQ=255)
                                                                                            aaGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                                                                                                                                               >  2:536632/1‑229 (MQ=255)
                                                                                             aGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCAcgtcgt                                                                                                                                                                                                                                   <  2:141097/208‑1 (MQ=255)
                                                                                                gcAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCa                                                                                                                                                                                                           <  4:536632/229‑1 (MQ=255)
                                                                                                                           aGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTTCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATAGCCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTACCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTgag                                                                                                                              >  1:77331/1‑279 (MQ=255)
                                                                                                                           aGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCAtt                                                                                                                                           >  2:366258/1‑266 (MQ=255)
                                                                                                                               attgattgATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTcc                                                                                                                                       <  4:366258/266‑1 (MQ=255)
                                                                                                                                            cTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATTCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGCGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCCCCAATTCCGCa                                                                                                             >  1:20128/1‑279 (MQ=255)
                                                                                                                                             tCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGCAGACGTTCAGTTCAAAGTTGCCGGCAGCGCCCCCCATGTTGATCGCCACGTAGTTCCACCTCACCTCACAGCCGAGCATGGTTAATGCCTCACACGTTGGTGGGTTCACTTTCCCCGGCATGATTGCGTAGCCCGGCNNNATTTCCGGGGTTGGTATTTTAACCATTCCGcgg                                                                                                            >  3:187626/1‑277 (MQ=255)
                                                                                                                                                             tACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGCGATTTCCCCAATTCCGCAGCGCAGGCCagcg                                                                                                >  4:218182/1‑273 (MQ=255)
                                                                                                                                                             tACCCACTGCGCAGTGTTTGTTAAAACTTTCAATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGCGGATCACCATGGGATGGAAGACGTTCAGTTCAAAGTTCCCGGAACCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACATGCCAGCAGCGCTTGGTTAATGCCTCACCCTGTGTTCGTTTCACTTTCCCCGGCCTGATTCAGCTTCCCGGCCCATCTGCCGGGACACAGATTGCACAACTTCGGCACCGTGGCGCagag                                                                                                >  4:12980/1‑275 (MQ=255)
                                                                                                                                                                  aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTcc                                                                                                                                                                                                                                >  2:600211/1‑142 (MQ=255)
                                                                                                                                                                    tGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCg                                                                                     >  1:101790/1‑279 (MQ=255)
                                                                                                                                                                     gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCAcg                                                                                                                                                                                                                                       >  4:597242/1‑132 (MQ=255)
                                                                                                                                                                      cgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCAt                                                                                                                                                                                                                            <  4:600211/142‑1 (MQ=255)
                                                                                                                                                                         aGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCAcgtcgt                                                                                                                                                                                                                                   <  2:597242/132‑1 (MQ=255)
                                                                                                                                                                                gttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAAt                                                                                                                   >  2:527367/1‑237 (MQ=255)
                                                                                                                                                                                gttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGAAGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTTACAGCAGAGCATTGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGAGTGATTTCGCTGCCCGGCCCATTTTCCGGGATTGACATTTCACCAATTCCCCCGCGCGCGCCAGATGCCAGCCCGCAGAcatcag                                                                             >  4:20646/1‑274 (MQ=255)
                                                                                                                                                                                    aaaaTTTCCAGGCCAGCATCCAGCAAGCGAACCGATAGCAGGCAATTGTTGTTCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCACATGTGGATCGCCACGTCGGTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCg                                                                                                               <  4:527367/237‑1 (MQ=255)
                                                                                                                                                                                         ttCCATGCCATCTGCCAGCATGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGACCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGCGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGAttt                                                                    >  2:410354/1‑275 (MQ=255)
                                                                                                                                                                                         ttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGCGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTtca                                                                 >  1:55809/1‑278 (MQ=255)
                                                                                                                                                                                         ttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGCtt                                                                     >  2:385869/1‑274 (MQ=255)
                                                                                                                                                                                                  accggcaggCAAGCGCACCGATTTCAGGAAATTGGGGAGTACCATTGGACGGACGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGc                                                       <  1:187626/271‑1 (MQ=255)
                                                                                                                                                                                                                 cACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCaa                                        >  1:143798/1‑279 (MQ=255)
                                                                                                                                                                                                                                        ccACCATGGGACGGAAGCGGTTCAGTTCAAAGTTGCCCGAAGCGCCCCCCCTTTTTATCGCCACGCCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAAc                      <  4:109581/273‑1 (MQ=255)
                                                                                                                                                                                                                                         cACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCCGCCAACCCTTTCAACGCGCCGt                                >  4:78514/1‑263 (MQ=255)
                                                                                                                                                                                                                                          aCCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGc                                                       >  4:440418/1‑239 (MQ=255)
                                                                                                                                                                                                                                             aTTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTgcgc                            <  2:78514/263‑1 (MQ=255)
                                                                                                                                                                                                                                              ttGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGcc                                                   <  2:440418/239‑1 (MQ=255)
                                                                                                                                                                                                                                                      aaGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCa                                                                                                                                                                                                                          <  3:124758/64‑1 (MQ=255)
                                                                                                                                                                                                                                                      aaGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCa                                                                                                                                                                                                                          >  1:124758/1‑64 (MQ=255)
                                                                                                                                                                                                                                                      aaGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCCTGATTGAGCTGCCCGGCTCATTTTCCGGGTTTGAGATTTCACCAATTCCGCAGCGCGGTCCAGAGGCCAGCCAGCGNACATCATTGCCGATTGTCTACAGGCACGCAGACACCCCCTTCAACGCGCCTGGCGCCTGAACCAGGCCaggcacggccccc   >  3:136139/1‑267 (MQ=255)
                                                                                                                                                                                                                                                      aaGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGACAACCCTTTCAACGCACCGTACGCCTGAACCAggtactcaccggt       >  4:493838/1‑264 (MQ=255)
                                                                                                                                                                                                                                                      aaGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCACCAGTGACGCAGCAAACCCTTTCAACGCGCCGTCCGCCTGAACCAGGGCATACCAggc       >  4:65181/1‑274 (MQ=255)
                                                                                                                                                                                                                                                           gttcagttcaAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTTCGCCTGAACCAGGGCATCACAGGTCGCCa  >  2:311586/1‑275 (MQ=255)
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CCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCA  >  NC_000913/1685261‑1685786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: