Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,145,313 C→A 100% S683Y (TCC→TAC)  yegE → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,145,3130CA100.0% 244.5 / ‑6.7 71S683Y (TCC→TAC) yegEputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (35/34);  total (35/36)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGT  >  NC_000913/2145059‑2145581
                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                            
tCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTTCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCAt                                                                                                                                                                                                                                                           >  2:133257/1‑274 (MQ=255)
       cTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTTCAGCGCCTACCATGATGCCATAACGACTCTCGCCaa                                                                                                                                                                                                                                                   >  4:513028/1‑275 (MQ=255)
         gACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCCCCCCGCCAAcc                                                                                                                                                                                                                                                 >  2:173062/1‑275 (MQ=255)
                          cTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGaaa                                                                                                                                                                                                                                >  2:432519/1‑275 (MQ=255)
                             ttGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTTGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAaacaac                                                                                                                                                                                                                            >  1:12913/1‑276 (MQ=255)
                                              ttaagtgagaACATTTACAATGCCGACACGTCACGTTCCGCGATCGGACAATAGGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACATTATGACGCCGTTAAGTACTCTGGACGGCAGACATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGCGAAACAACTGCGTATCCtgctgc                                                                                                                                                                                                             <  4:25395/268‑1 (MQ=255)
                                                    gggaACATTTACAGTGCCGACACCTCAGGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGGCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGt                                                                                                                                                                                                      <  4:357056/273‑1 (MQ=255)
                                                    gagaACATTTACAGTGCCGACACCTCTCGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                   <  1:61974/278‑1 (MQ=255)
                                                    gagaACATTTACAGTGCCGACACCTCACGGTCCGCGAGCGAGCAAGAGGGGGGGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACGCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATGCAGGACGTTACCGAATCACGCAAAAGGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGt                                                                                                                                                                                                      <  2:6702/275‑1 (MQ=255)
                                                    aaggatatgtACGGTGCCGACACTCTCCGTTCCGGGGTCGAGAAAGATGGGGTGTTGCACGTGCGGAGCGGCGGCAGTTANGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                   <  3:138098/269‑1 (MQ=255)
                                                        aCATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                   <  2:459291/274‑1 (MQ=255)
                                                        aCATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCGCGCCAACCCCTCCAGTTTTCAGAAACCACTTCGTATCCTGATGCAAACGGTaaa                                                                                                                                                                                                   >  2:301125/1‑274 (MQ=255)
                                                           tttACAGGGCCGAAACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGt                                                                                                                                                                                               <  2:513028/275‑1 (MQ=255)
                                                                   tccGACCTCTCACGTTCCGCGATTGGGCAAGAGTTGAGGTTTCACTGACGGAGATGAGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGGCGGCAGCAATATTGGTTCGGATCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCGACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATc                                                                                                                                                                                        <  4:133257/273‑1 (MQ=255)
                                                                   gCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACCGCGCCTACCATGATGCACTGACGCCTCTCCCCAACCACGCCAGTTTTGAGAAACCACTGCGTATCCTGCCTCAAACGGTAAACAGTaccaaacc                                                                                                                                                                                       >  4:119783/1‑269 (MQ=255)
                                                                   gCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                   >  1:138098/1‑263 (MQ=255)
                                                                    ccGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCACTATTGGTTCGGTTCTGATGATTCAGGACCTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCCACCGCGCCAATTTTGAGAAACAACTCGGTAGTCTGCAGCAAACGGTAAAcgatcaacctcaa                                                                                                                                                                                      >  4:192142/1‑263 (MQ=255)
                                                                    ccGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTg                                                                                                                                                                                                                                     >  1:101148/1‑228 (MQ=255)
                                                                    ccGACACCCCACGTTCCGCGATCCAGCAAGATGTGGTGTTGCACTGTCGTAGCGGCGGCAGTTACGACGTGCATTACAGTATTACCCCGCTACGTACCCTGGACGGTAGCAATATTGGTTGGGTTCTGGTGATTCAGGACTTCTCCTAATCACGAAGAAGGCTGCGCCAGCCAAGCAAAAGCGCCTACCATGAATCAATGACGAACCGCGCCAACCGCCCCCGTTTTGATCAAAAACTGCGTATCCGGCCGCAAACGGTTAAccttacccctaa                                                                                                                                                                                       >  4:459084/1‑263 (MQ=255)
                                                                        cacCTCACGTTCCGCGATTGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCtgctgc                                                                                                                                                                                                             <  2:459084/248‑1 (MQ=255)
                                                                        cacCTCACGTTCCGCGATCGAGCAATGTGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACGCTGGACGGCAGCAATAGTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc                                                                                                                                                                                  <  2:76970/275‑1 (MQ=255)
                                                                              aaCGTTCCGCGATCGAGCAAGATGTGGTGTTGCACGTTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGcc                                                                                                                                                                             <  2:559489/274‑1 (MQ=255)
                                                                                 gTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCt                                                                                                                                                                           <  2:233314/274‑1 (MQ=255)
                                                                                  ttCCGCGTTCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTg                                                                                                                                                                          <  2:54483/274‑1 (MQ=255)
                                                                                  ttCCGCGATCGAGCAAGAAGGTGTGGTGCACTGGCGGTGCTGCGGCAGTTGCGACGTGCATTACAGTATTACGCCGTTAAGTGCTCTGGACAGGAGGAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCt                                                                                                                                                                           <  2:413239/273‑1 (MQ=255)
                                                                                      gcgATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCCACCGCGCCAGTTTTGAGAAACAACTCCGTATCCTGATGCACACGGTACACATTCCCCACCAGCCACATGCCATGGTGtt                                                                                                                                                                     >  2:449259/1‑275 (MQ=255)
                                                                                                    tgtgGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAAc                                                                                                                                                                                                  >  4:45907/1‑232 (MQ=255)
                                                                                                        gtgtTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                                                                                                                                              <  2:45907/232‑1 (MQ=255)
                                                                                                         tgtTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCACCCGCCCCAGTTTTGAGACACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGCCATGCCCTGGTGTTTACCGACCTTTATCCCttt                                                                                                                                                  >  4:428559/1‑275 (MQ=255)
                                                                                                           ttGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGCTGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGaaa                                                                                                                                                                                                                                >  1:126958/1‑195 (MQ=255)
                                                                                                              cACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTgaga                                                                                                                                                                                                                                  >  2:429564/1‑190 (MQ=255)
                                                                                                              cACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCCATCTTg                                                                                                                                                          >  4:306351/1‑262 (MQ=255)
                                                                                                                cTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTa                                                                                                                                                                   <  4:252599/251‑1 (MQ=255)
                                                                                                                cTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTa                                                                                                                                                                   >  2:252599/1‑251 (MQ=255)
                                                                                                                  gTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAaaca                                                                                                                                                                                                                              <  4:429564/190‑1 (MQ=255)
                                                                                                                  gTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCg                                                                                                                                                      <  2:306351/262‑1 (MQ=255)
                                                                                                                   tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTg                                                                                                                                         >  2:328735/1‑274 (MQ=255)
                                                                                                                       aGCTGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTGCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATg                                                                                                                                     <  2:10937/274‑1 (MQ=255)
                                                                                                                                            cATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTTGTTCGGTTCTGGTGATTCAGGACGTTACCGACTCACGCACAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTCAAGCGGTGAATGCCAGCGCCGGGCATGCGGAggg                                                                                                               >  2:573476/1‑275 (MQ=255)
                                                                                                                                                       tACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTTAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTCTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAAACGTACACATCAGCGACATGCCCTGGTGTTTAACGATCTTGATCGCTTTAAAGCCGTAAATGACGGCGCCGGGCATGCGGCGGGTGAAGcctg                                                                                                      >  4:141930/1‑270 (MQ=255)
                                                                                                                                                           ccGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATgcg                                                                                                                    <  2:141930/255‑1 (MQ=255)
                                                                                                                                                              ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGa                                                                                                                                        >  4:254539/1‑232 (MQ=255)
                                                                                                                                                              ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTAAGAAACAACTGCGTATCCTGCTGCAATCGGTAAACAGTACACATCAGCGAAATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGAAGCTTTGCTGCGCGa                                                                                             >  2:497264/1‑275 (MQ=255)
                                                                                                                                                                  gTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGa                                                                                                                                    <  2:254539/232‑1 (MQ=255)
                                                                                                                                                                   tACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATTGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGaa                                                                                            <  2:119783/271‑1 (MQ=255)
                                                                                                                                                                    accctGGACGGCAGCAAAATTGGTTCGGTTCGGGGGATTCAGGACGTTACCGAATCACGAAAAATGCTGCCCCAGCTGCGCTACAGCGCCTACCATGAGGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGc                                                                                       <  4:173062/272‑1 (MQ=255)
                                                                                                                                                                     ctctGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCTCTCGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGCAATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCAGGTGACGCTTTGCTGCGCGAACTGGcg                                                                                      >  2:82686/1‑275 (MQ=255)
                                                                                                                                                                        tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGAAGCTTTGCTGCGCGAACTGGCGTCGTTaa                                                                               >  1:179278/1‑279 (MQ=255)
                                                                                                                                                                           aCGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGcgtcg                                                                                   >  2:410822/1‑272 (MQ=255)
                                                                                                                                                                              tgagcaATTTTGTTTGGGTTTTGGGGATTCANNNCCTTACCGAATCACGCAAAAAGCGGCGCCAGCTGGGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCt                                                                           <  3:179278/275‑1 (MQ=255)
                                                                                                                                                                                          gtttgtttcTGGGGATTCAGGACGTTACCGAATCACGGAAAATGCTGCGCCCGCTGAGCTCCATCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCCAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTAtgctg                                                                 <  4:493768/269‑1 (MQ=255)
                                                                                                                                                                                            tcggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGa                                                                                                                                    <  1:78527/206‑1 (MQ=255)
                                                                                                                                                                                            tcggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGa                                                                                                                                    >  3:78527/1‑206 (MQ=255)
                                                                                                                                                                                             cggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGt                                                              >  2:147755/1‑275 (MQ=255)
                                                                                                                                                                                               gttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATc                                                                                                                                                             >  2:197131/1‑178 (MQ=255)
                                                                                                                                                                                                  cTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAg                                                         <  2:411864/275‑1 (MQ=255)
                                                                                                                                                                                                   tGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGa                                                                                                                                                         <  4:197131/178‑1 (MQ=255)
                                                                                                                                                                                                    ggTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCCCGCCAACCGCGCCAGTTTTTAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACCTGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTACCGCGAGAACTCGCGTCGTTAATGCTGAGTAttcccgtcccttat                                                        >  4:470624/1‑261 (MQ=255)
                                                                                                                                                                                                        aTTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAAt                                                                              <  2:470624/248‑1 (MQ=255)
                                                                                                                                                                                                          tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATTCCCTGGTGTTTATCGATCTCGCTCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGAATCTTTGCTGCGCGAACTCGCGTGGTTAATGCTGAGTATGTTGCGTTCCAGCGACGTGc                                                 >  2:262261/1‑275 (MQ=255)
                                                                                                                                                                                                          tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATc                                                                                                                                                             >  2:591457/1‑167 (MQ=255)
                                                                                                                                                                                                          tCAGGACGTTACCGAATCACGCAAAATGCTGCCCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGAACTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGTGACGtcc                                                 >  2:133381/1‑273 (MQ=255)
                                                                                                                                                                                                              gACGTTACCGAATCNCGCANAATGCTGCGCCAGCTGAGCTACANCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGa                                                                                                                                                         <  4:591457/167‑1 (MQ=255)
                                                                                                                                                                                                                 gTTACCGAATCACGCAAAATGCTGCGCAAGCTGAGCGACAGCGCCTACCATGATGCACGGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACt                                       <  1:175287/278‑1 (MQ=255)
                                                                                                                                                                                                                   tACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGAGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGCCGTTCTGgcgct                                          >  2:448470/1‑272 (MQ=255)
                                                                                                                                                                                                                   tACCGAATCACGCAAAATGCTGCGCCAGATGAGCTACCGCGCCTACCATGATGCACTGACGCATCCCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACCTGCCCTGGTGTTTATCGATCTTGATCGCTTTACAGCGGTGAATGACAGCGCCGGGAATGCGGCCGGTGAAGCTTTGCGGCGCGCACTGGCTGCGGTAATGATGACTATTCTGCGTCCCACCGACGTTGTGGCGCCAc                                        >  4:480087/1‑275 (MQ=255)
                                                                                                                                                                                                                        aaTCACACAAAACGCAGAGACAGCTGAGCGACAGTGCCTACCCTGATTGACTGAAGCATCGTGCCAACCGCTCCAGGTGTGAGAAACAACGGCGTAGCCGGCTGCAAAAGGTAAACAGCACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGCTCGCTTTAAAGCGACGAATGACAGCGACGGGCATGCGGCGGGTGACGTGTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCTGCGACGTGCTGGCGCGACTCGGt                                   <  4:573476/275‑1 (MQ=255)
                                                                                                                                                                                                                                           ccAGCTGAGGTACAGCGCCGCCCAGGATGCACTGACGCCACTCGCCAACCGTGCCAGTTTTGAGAAACAACTGAGGATCCTGCTGCAAACTGTAAACAGTACACATCAGGGACATGCCCGTGTGTTTATCGATCTTGATCGCTTTAAAGCGTGTAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc                <  4:328735/275‑1 (MQ=255)
                                                                                                                                                                                                                                                    cTACAGCGCCTACCATGATGCCCTGACGCATCTCGCCAACCGCGCCAGTTTTGTGAAACAACTGCGTATCCTGCTGCAAACGGTAAACCGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCa        <  2:286087/274‑1 (MQ=255)
                                                                                                                                                                                                                                                      aCAGAGCCTCCCAGAATGCACTTACGGTGCTCGCCACCCGGTCCAGTTTTGAGAAACAACTGCGGATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGa      <  4:410822/274‑1 (MQ=255)
                                                                                                                                                                                                                                                         gtgcCTACCATGTGTCACTGCCGAATCTGCCCAGCCGTGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGGGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGt  <  4:432519/273‑1 (MQ=255)
                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                            
TCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGT  >  NC_000913/2145059‑2145581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: