Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,679,400 G→C 13.6% intergenic (+29/‑157) ydgH → / → ydgI DUF1471 family periplasmic protein/putative arginine/ornithine antiporter transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,679,4000GC13.6% 203.2 / 24.2 66intergenic (+29/‑157)ydgH/ydgIDUF1471 family periplasmic protein/putative arginine/ornithine antiporter transporter
Reads supporting (aligned to +/- strand):  ref base G (29/28);  new base C (5/4);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.12e-01

AAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTAATCACT  >  NC_000913/1679253‑1679548
                                                                                                                                                   |                                                                                                                                                    
aaGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGa                                                                                                                                                     <  3:504323/149‑1 (MQ=255)
    ctcCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATgcc                                                                                                                                                 <  2:332500/149‑1 (MQ=255)
        tATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCt                                                                                                                                             >  2:530917/1‑149 (MQ=255)
                     aaCGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAg                                                                                                                                <  1:530917/149‑1 (MQ=255)
                             gcgAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAAt                                                                                                                        <  1:664224/149‑1 (MQ=255)
                                aagaaAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTc                                                                                                                     <  2:1100305/149‑1 (MQ=255)
                                aagaaAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTc                                                                                                                     <  3:974086/149‑1 (MQ=255)
                                aagaaAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTc                                                                                                                     >  1:566981/1‑149 (MQ=255)
                                  gaaAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTc                                                                                                                                          <  2:1044750/126‑1 (MQ=255)
                                  gaaAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTc                                                                                                                                          >  1:1044750/1‑126 (MQ=255)
                                   aaaGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTcccc                                                                                                                  >  2:1039394/1‑149 (MQ=255)
                                   aaaGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTcccc                                                                                                                  >  4:384464/1‑149 (MQ=255)
                                               tATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtgca                                                                                                      <  3:384464/149‑1 (MQ=255)
                                               tATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtgca                                                                                                      >  4:360495/1‑149 (MQ=255)
                                                aTTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTa                                                                                                                                 <  3:680787/121‑1 (MQ=255)
                                                aTTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtgcat                                                                                                     <  1:1039394/149‑1 (MQ=255)
                                                aTTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTAACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTa                                                                                                                                 >  4:680787/1‑121 (MQ=255)
                                                 ttACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtgcatg                                                                                                    >  1:592272/1‑149 (MQ=255)
                                                    ccACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTTCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAg                                                                                                 >  1:1026539/1‑149 (MQ=255)
                                                    ccACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAg                                                                                                 >  1:430088/1‑149 (MQ=255)
                                                     cacaTCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGAt                                                                                                                                                    <  1:1119040/97‑1 (MQ=255)
                                                     cacaTCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGAt                                                                                                                                                    >  2:1119040/1‑97 (MQ=255)
                                                           aCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACAtc                                                                                          >  3:401555/1‑149 (MQ=255)
                                                             ccGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACAtctc                                                                                        >  2:200760/1‑149 (MQ=255)
                                                             ccGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACAtctc                                                                                        >  2:398788/1‑149 (MQ=255)
                                                              cGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATctct                                                                                       >  1:228868/1‑149 (MQ=255)
                                                                        aGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATc                                                                             >  2:1009533/1‑149 (MQ=255)
                                                                              gTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTGTAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCAtt                                                                                                         >  1:667469/1‑115 (MQ=255)
                                                                              gTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTGTAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCAtt                                                                                                         <  2:667469/115‑1 (MQ=255)
                                                                                   aataaCCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAg                                                                  >  3:877680/1‑149 (MQ=255)
                                                                                    ataaCCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCAGTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGt                                                                 <  4:877680/149‑1 (MQ=255)
                                                                                     taaCCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTg                                                                <  4:982213/149‑1 (MQ=255)
                                                                                         cTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCATCAAGCCGCCttata                                                                                                                                         >  1:862825/1‑69 (MQ=37)
                                                                                         cTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCATCAAGCCGCCttata                                                                                                                                         <  2:862825/72‑4 (MQ=37)
                                                                                                       aGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATg                                              >  4:904049/1‑149 (MQ=255)
                                                                                                        gATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGc                                             <  2:1026539/149‑1 (MQ=255)
                                                                                                         aTCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCg                                            >  3:691624/1‑149 (MQ=255)
                                                                                                         aTCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTCTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGATACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCg                                            >  1:819450/1‑149 (MQ=255)
                                                                                                           cTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGtt                                          <  2:228868/149‑1 (MQ=255)
                                                                                                                 aaataaCTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGccc                                    <  2:566981/149‑1 (MQ=255)
                                                                                                                 aaataaCTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGccc                                    >  4:376965/1‑149 (MQ=255)
                                                                                                                    taaCTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTaa                                 >  2:599765/1‑149 (MQ=255)
                                                                                                                     aaCTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAat                                >  3:1142478/1‑149 (MQ=255)
                                                                                                                         aTTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGtt                                          >  2:105244/1‑135 (MQ=255)
                                                                                                                         aTTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGtt                                          <  1:105244/135‑1 (MQ=255)
                                                                                                                         aTTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGtt                                          <  1:779743/135‑1 (MQ=255)
                                                                                                                         aTTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGtt                                          >  2:779743/1‑135 (MQ=255)
                                                                                                                          ttCAGTTACTGCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTCTTGCCCTAAtattta                           <  2:819450/149‑1 (MQ=255)
                                                                                                                           tCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAAtatttat                          <  1:398788/149‑1 (MQ=255)
                                                                                                                           tCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAAtatttat                          <  1:200760/149‑1 (MQ=255)
                                                                                                                                 aCTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATtcttct                    <  2:430088/149‑1 (MQ=255)
                                                                                                                                  cTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTcttctt                   <  1:1009533/149‑1 (MQ=255)
                                                                                                                                     cTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTa                                                >  1:234360/1‑117 (MQ=255)
                                                                                                                                     cTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTa                                                <  2:234360/117‑1 (MQ=255)
                                                                                                                                     cTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATc                <  4:1142478/149‑1 (MQ=255)
                                                                                                                                     cTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATc                >  3:437696/1‑149 (MQ=255)
                                                                                                                                      tataAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATCa               <  2:592272/149‑1 (MQ=255)
                                                                                                                                       gaaaGGCGGCATCAAGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGccc                                    >  4:1142421/3‑127 (MQ=255)
                                                                                                                                       gaaaGGCGGCATCAAGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGccc                                    <  3:88918/125‑1 (MQ=255)
                                                                                                                                       gaaaGGCGGCATCAAGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGccc                                    <  3:1142421/125‑1 (MQ=255)
                                                                                                                                       gaaaGGCGGCATCAAGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGccc                                    >  4:88918/3‑127 (MQ=255)
                                                                                                                                       gaaaGGCGGCATCAAGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATCAc              >  4:528537/3‑149 (MQ=255)
                                                                                                                                          aGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATCACGtt           <  3:904049/149‑1 (MQ=255)
                                                                                                                                          aGGCGGCATCAAGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATtcttct                    >  2:60045/1‑140 (MQ=255)
                                                                                                                                          aGGCGGCATCAAGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATtcttct                    <  1:60045/140‑1 (MQ=255)
                                                                                                                                               gcTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTAAt      >  3:566215/1‑149 (MQ=255)
                                                                                                                                                   gATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTAATCACt  >  2:38447/1‑149 (MQ=255)
                                                                                                                                                   gATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTAATCACt  <  4:691624/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCGCGCTTCAGTGGCAAACTCCATTTTTATGCGTTTTGCCCTAATATTTATTCTTCTTATCACGTTTTAATCACT  >  NC_000913/1679253‑1679548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: