Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,737,438 C→T 5.4% intergenic (‑148/+18) ydhP ← / ← ynhF putative MFS transporter, inner membrane protein/stress response membrane

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,737,4380CT 5.4% 351.3 / 3.5 104intergenic (‑148/+18)ydhP/ynhFputative MFS transporter, inner membrane protein/stress response membrane
Reads supporting (aligned to +/- strand):  ref base C (48/50);  new base T (3/3);  total (51/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 109 total aligned reads displayed.

TGCCTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGATATT  >  NC_000913/1737290‑1737586
                                                                                                                                                    |                                                                                                                                                    
tGCCTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAAcgc                                                                                                                                                      <  2:543439/149‑1 (MQ=255)
  ccTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACgcgc                                                                                                                                                    <  2:1101256/149‑1 (MQ=255)
   cTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCt                                                                                                                                                   >  2:350433/1‑149 (MQ=255)
   cTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCt                                                                                                                                                   >  4:225286/1‑149 (MQ=255)
    ttATCTCGACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTc                                                                                                                                                  >  2:623165/1‑149 (MQ=255)
     tATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTcc                                                                                                                                                 <  3:190528/149‑1 (MQ=255)
     tATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTcc                                                                                                                                                 <  2:1060030/149‑1 (MQ=255)
        ctcCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGCGCGTTCTCCCTc                                                                                                                                              <  2:1139735/149‑1 (MQ=255)
        ctcCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTc                                                                                                                                              <  2:251830/149‑1 (MQ=255)
         tcCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCg                                                                                                                                             >  1:345128/1‑149 (MQ=255)
           cACCTCTTCGCGTCATTGCGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGATAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGCGGGAGAACGTGCTCCCTCgag                                                                                                                                           >  4:709020/1‑149 (MQ=255)
              ctctTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCgaga                                                                                                                                          <  3:352757/147‑1 (MQ=255)
              ctctTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCgaga                                                                                                                                          >  4:352757/1‑147 (MQ=255)
              ctctTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAgg                                                                                                                                        <  2:695694/149‑1 (MQ=255)
               tctTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGa                                                                                                                                       >  1:322853/1‑149 (MQ=255)
                 ttCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGaaa                                                                                                                                     >  4:574207/1‑149 (MQ=255)
                 ttCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGaaa                                                                                                                                     >  1:551256/1‑149 (MQ=255)
                 ttCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGaaa                                                                                                                                     >  2:875596/1‑149 (MQ=255)
                  tCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAAt                                                                                                                                    <  3:225286/149‑1 (MQ=255)
                     cgTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAg                                                                                                                                 <  1:875596/149‑1 (MQ=255)
                      gTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGt                                                                                                                                >  1:198797/1‑149 (MQ=255)
                                 atatTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCa                                                                                                                     <  3:574207/149‑1 (MQ=255)
                                   atTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGt                                                                                                                   <  3:537649/149‑1 (MQ=255)
                                       aTTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCaaa                                                                                                               >  2:339200/1‑149 (MQ=255)
                                       aTTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCaaa                                                                                                               <  1:350433/149‑1 (MQ=255)
                                        ttAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAAc                                                                                                              >  3:115398/1‑149 (MQ=255)
                                        ttAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAAc                                                                                                              >  4:80269/1‑149 (MQ=255)
                                        ttAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAAc                                                                                                              >  1:335683/1‑149 (MQ=255)
                                                gCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCt                                                                                                      <  1:623165/149‑1 (MQ=255)
                                                  gAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTAc                                                                                                    >  4:1136668/1‑149 (MQ=255)
                                                          gACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAAAGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAAc                                                                                            >  2:564303/1‑149 (MQ=255)
                                                             aGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGa                                                                                         >  2:867184/1‑149 (MQ=255)
                                                                aaTCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGa                                                                                      <  2:35286/149‑1 (MQ=255)
                                                                aaTCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGa                                                                                      >  3:424097/1‑149 (MQ=255)
                                                                 aTCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGAc                                                                                     >  4:257991/1‑149 (MQ=255)
                                                                    acaAAAAAATGGAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGAt                                                                                  >  4:1108127/1‑149 (MQ=255)
                                                                      aaaaaaaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATaa                                                                                >  2:678503/1‑149 (MQ=255)
                                                                        aaaaaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATc                                                                              >  3:490756/1‑149 (MQ=255)
                                                                         aaaaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAATAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCg                                                                             <  1:339200/149‑1 (MQ=255)
                                                                          aaaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgt                                                                            <  4:115398/149‑1 (MQ=255)
                                                                           aaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgtt                                                                           <  4:490756/149‑1 (MQ=255)
                                                                           aaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgtt                                                                           <  1:879786/149‑1 (MQ=255)
                                                                           aaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgtt                                                                           <  2:984386/149‑1 (MQ=255)
                                                                           aaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATGTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCCACGATCAAACCGAGGACGATAATCgtt                                                                           <  3:763945/149‑1 (MQ=255)
                                                                            aTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgttg                                                                          >  1:964079/1‑149 (MQ=255)
                                                                                aaaTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgttg                                                                          >  3:829731/1‑145 (MQ=255)
                                                                                aaaTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgttg                                                                          <  4:829731/145‑1 (MQ=255)
                                                                                  aTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCa                                                                    >  1:769728/1‑149 (MQ=255)
                                                                                    aaCAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGt                                                                  >  2:892845/1‑149 (MQ=255)
                                                                                    aaCAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGt                                                                  >  1:513071/1‑149 (MQ=255)
                                                                                    aaCAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAAAGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGt                                                                  <  1:564303/149‑1 (MQ=255)
                                                                                     aCAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGa                                                                                         <  1:738617/125‑1 (MQ=255)
                                                                                     aCAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTg                                                                 >  3:1064983/1‑149 (MQ=255)
                                                                                        aaaaGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAATGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGaaa                                                              >  2:810343/1‑149 (MQ=255)
                                                                                         aaaGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTcc                                                                                                                                                 <  4:204414/65‑1 (MQ=255)
                                                                                         aaaGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTcc                                                                                                                                                 >  3:204414/1‑65 (MQ=255)
                                                                                           agagAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAAtt                                                           >  3:149202/1‑149 (MQ=255)
                                                                                              gaAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTaa                                                        <  1:867184/149‑1 (MQ=255)
                                                                                              gaAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTaa                                                        >  1:1019225/1‑149 (MQ=255)
                                                                                               aaaaCACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAg                                                       >  2:297540/1‑149 (MQ=255)
                                                                                                 aaCACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCACTGAAAATTTAAGGt                                                     <  3:80269/149‑1 (MQ=255)
                                                                                                  acacTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACGCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTc                                                    <  2:937500/149‑1 (MQ=255)
                                                                                                    acTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCgg                                                  >  2:1070656/1‑149 (MQ=255)
                                                                                                    acTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCgg                                                  <  3:426932/149‑1 (MQ=255)
                                                                                                            cATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCa                                                                    >  2:913456/1‑123 (MQ=255)
                                                                                                            cATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCa                                                                    <  1:913456/123‑1 (MQ=255)
                                                                                                              ttttGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAg                                                                                        >  3:844120/1‑101 (MQ=255)
                                                                                                              ttttGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAg                                                                                        <  4:844120/101‑1 (MQ=255)
                                                                                                               tttGCTAATAAACATGAAGGAGATGCGAGGGTGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTCCGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAg                                       <  3:709020/149‑1 (MQ=255)
                                                                                                                 tGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGtt                                     <  3:257991/149‑1 (MQ=255)
                                                                                                                   cTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGtttt                                   <  1:393756/149‑1 (MQ=255)
                                                                                                                    tAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgttgt                                                                         <  3:575556/110‑1 (MQ=255)
                                                                                                                    tAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgttgt                                                                         >  4:575556/1‑110 (MQ=255)
                                                                                                                    tAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtc                                  <  2:551256/149‑1 (MQ=255)
                                                                                                                        aaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCa                                                                    <  4:839517/111‑1 (MQ=255)
                                                                                                                        aaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCa                                                                    >  3:839517/1‑111 (MQ=255)
                                                                                                                        aaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtctc                                <  4:249319/147‑1 (MQ=255)
                                                                                                                        aaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTCTtctc                                >  3:249319/1‑147 (MQ=255)
                                                                                                                             ggaaggaGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCtttttt                         <  2:345128/149‑1 (MQ=255)
                                                                                                                             ggaaggaGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCtttttt                         <  1:678503/149‑1 (MQ=255)
                                                                                                                                 ggaGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCttttt                          >  4:645350/1‑144 (MQ=255)
                                                                                                                                 ggaGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCttttt                          <  3:645350/144‑1 (MQ=255)
                                                                                                                                 ggaGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTttatta                     <  3:1136668/149‑1 (MQ=255)
                                                                                                                                  gagaTGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTAc                    >  4:523262/1‑149 (MQ=255)
                                                                                                                                  gagaTGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTAc                    <  4:149202/149‑1 (MQ=255)
                                                                                                                                   agaTGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTAcc                   >  2:1138003/1‑149 (MQ=255)
                                                                                                                                     tctcGAGGGAGCGCGTTCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACcac                 >  1:242771/4‑149 (MQ=255)
                                                                                                                                     tctcGAGGGAGCGCGTTCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACcac                 >  3:233193/4‑149 (MQ=255)
                                                                                                                                      ctcGAGGGAGCGCGTTCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACcaca                <  4:233193/147‑1 (MQ=255)
                                                                                                                                          aGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACaaa            >  2:1059294/1‑149 (MQ=255)
                                                                                                                                          aGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACaaa            >  2:115857/1‑149 (MQ=255)
                                                                                                                                            ggAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACaaaaa          <  4:1064983/149‑1 (MQ=255)
                                                                                                                                              agaACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGt        <  2:769728/149‑1 (MQ=255)
                                                                                                                                               gaACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTg       >  2:675956/1‑149 (MQ=255)
                                                                                                                                                aaCGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGa      <  1:115857/149‑1 (MQ=255)
                                                                                                                                                aaCGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGa      <  1:1059294/149‑1 (MQ=255)
                                                                                                                                                 aCGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGat     <  1:675956/149‑1 (MQ=255)
                                                                                                                                                   gcgcTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACCAAAAGTGatat   <  1:1076812/149‑1 (MQ=255)
                                                                                                                                                    cgcTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGATAtt  <  4:424097/149‑1 (MQ=255)
                                                                                                                                                    cgcTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGATAtt  <  3:1108127/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGCCTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGATATT  >  NC_000913/1737290‑1737586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑