Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,778,372 C→G 6.9% intergenic (+25/+18) ydiO → / ← ydiP putative acyl‑CoA dehydrogenase/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,778,3720CG 6.9% 204.5 / 6.9 58intergenic (+25/+18)ydiO/ydiPputative acyl‑CoA dehydrogenase/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (28/26);  new base G (2/2);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-01

GATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTG  >  NC_000913/1778225‑1778518
                                                                                                                                                   |                                                                                                                                                  
gATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACa                                                                                                                                                   >  3:333798/1‑149 (MQ=255)
  tGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACaaa                                                                                                                                                 <  4:644078/149‑1 (MQ=255)
  tGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACaaa                                                                                                                                                 >  3:239265/1‑149 (MQ=255)
      gcgcgcGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGTCTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTgc                                                                                                                                             <  1:743928/149‑1 (MQ=255)
      gcgcgcGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTgc                                                                                                                                             <  3:930224/149‑1 (MQ=255)
         cgcgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGcgcc                                                                                                                                          <  1:531586/149‑1 (MQ=255)
                  cGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGAttt                                                                                                                                 >  3:950548/1‑149 (MQ=255)
                   gCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTa                                                                                                                                >  4:226375/1‑149 (MQ=255)
                     ttCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATc                                                                                                                              <  3:226375/149‑1 (MQ=255)
                          gCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGAttt                                                                                                                                 >  1:658425/1‑141 (MQ=255)
                          gCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGAttt                                                                                                                                 <  2:658425/141‑1 (MQ=255)
                          gCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGAt                                                                                                                                   <  1:354327/139‑1 (MQ=255)
                          gCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGAt                                                                                                                                   >  2:354327/1‑139 (MQ=255)
                          gCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCcaaca                                                                                                                         >  2:839927/1‑149 (MQ=255)
                                 gTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTa                                                                                                                  >  3:719648/1‑149 (MQ=255)
                                 gTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTa                                                                                                                  >  2:447030/1‑149 (MQ=255)
                                  tCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTAc                                                                                                                 <  4:333798/149‑1 (MQ=255)
                                  tCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTAc                                                                                                                 <  1:839927/149‑1 (MQ=255)
                                      ttGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAATGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGt                                                                                                             <  4:719648/149‑1 (MQ=255)
                                          gAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAg                                                                                                         >  4:1076687/1‑149 (MQ=255)
                                          gAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAg                                                                                                         <  3:425616/149‑1 (MQ=255)
                                            aCGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTTTCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGa                                                                                                       >  1:528978/1‑149 (MQ=255)
                                                aTCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAAtt                                                                                                   <  4:950548/149‑1 (MQ=255)
                                                 tCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTTTCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTc                                                                                                  <  2:528978/149‑1 (MQ=255)
                                                                aCGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGaaa                                                                                                     >  4:17523/1‑131 (MQ=255)
                                                                aCGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGaaa                                                                                                     <  3:17523/131‑1 (MQ=255)
                                                                 cGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGt                                                                                  >  3:339762/1‑149 (MQ=255)
                                                                  gAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTg                                                                                   <  4:1012477/147‑1 (MQ=255)
                                                                  gAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTg                                                                                   >  3:1012477/1‑147 (MQ=255)
                                                                  gAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGtt                                                                                 >  2:84252/1‑149 (MQ=255)
                                                                    aaTTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTcc                                                                               <  4:239265/149‑1 (MQ=255)
                                                                            tttACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACa                                                                       <  1:447030/149‑1 (MQ=255)
                                                                            tttACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACa                                                                       >  2:710485/1‑149 (MQ=255)
                                                                                    gCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTaa                                                               >  1:268230/1‑149 (MQ=255)
                                                                                     cAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACTAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTaaa                                                              <  1:84252/149‑1 (MQ=255)
                                                                                          cGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTc                                                         <  1:710485/149‑1 (MQ=255)
                                                                                               gATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACgg                                                    >  1:1137573/1‑149 (MQ=255)
                                                                                                 tCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTa                                                  >  3:459837/1‑149 (MQ=255)
                                                                                                             aTCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttt                                                                   <  1:913/120‑1 (MQ=255)
                                                                                                             aTCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttt                                                                   >  2:913/1‑120 (MQ=255)
                                                                                                              tCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGaa                                     >  1:625837/1‑149 (MQ=255)
                                                                                                                 gAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTg                                           >  1:715979/1‑140 (MQ=255)
                                                                                                                 gAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTg                                           <  2:715979/140‑1 (MQ=255)
                                                                                                                 gAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGaacaa                                  >  1:820225/1‑149 (MQ=255)
                                                                                                                        taatCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACAt                           <  2:820225/149‑1 (MQ=255)
                                                                                                                                aGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCaaa                   >  1:916707/1‑149 (MQ=255)
                                                                                                                                  gcggcgCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCaaaaa                 <  3:1076687/149‑1 (MQ=255)
                                                                                                                                     gcgcAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAg              >  1:754317/1‑149 (MQ=255)
                                                                                                                                       gcAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGtt            >  1:44608/1‑149 (MQ=255)
                                                                                                                                       gcAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGa                              >  3:907969/1‑131 (MQ=255)
                                                                                                                                       gcAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGa                              <  4:907969/131‑1 (MQ=255)
                                                                                                                                        cAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGttt           <  2:625837/149‑1 (MQ=255)
                                                                                                                                         aGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTg          <  4:339762/149‑1 (MQ=255)
                                                                                                                                         aGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTg          >  4:552254/1‑149 (MQ=255)
                                                                                                                                           cttcttAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGc        <  3:552254/149‑1 (MQ=255)
                                                                                                                                                 aaCAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTg  >  3:907418/1‑149 (MQ=255)
                                                                                                                                                  aCAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGa                              <  4:135372/120‑1 (MQ=255)
                                                                                                                                                  aCAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGa                              >  3:135372/1‑120 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTG  >  NC_000913/1778225‑1778518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑