Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,948,179 T→A 7.9% intergenic (‑28/+1) yebC ← / ← nudB UPF0082 family protein/dihydroneopterin triphosphate pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,948,1790TA 7.9% 308.4 / 13.6 89intergenic (‑28/+1)yebC/nudBUPF0082 family protein/dihydroneopterin triphosphate pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base T (40/42);  new base A (4/3);  total (44/45)
Fisher's exact test for biased strand distribution p-value = 7.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

ACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGAA  >  NC_000913/1948032‑1948326
                                                                                                                                                   |                                                                                                                                                   
aCCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGtt                                                                                                                                                    <  3:33326/149‑1 (MQ=255)
  cGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCa                                                                                                                                                  <  3:961116/149‑1 (MQ=255)
   gCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAg                                                                                                                                                 <  3:749471/149‑1 (MQ=255)
    cccAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAgg                                                                                                                                                >  3:507958/1‑149 (MQ=255)
     ccAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGc                                                                                                                                               >  2:1066405/1‑149 (MQ=255)
      cAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCa                                                                                                                                              <  3:46739/149‑1 (MQ=255)
        gCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGc                                                                                                                                            <  4:48101/149‑1 (MQ=255)
             gCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTaa                                                                                                                                       >  2:778269/1‑149 (MQ=255)
             gCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTaa                                                                                                                                       <  1:537621/149‑1 (MQ=255)
                 cgGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTAc                                                                                                                                   <  4:507958/149‑1 (MQ=255)
                    ttACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACaaa                                                                                                                                <  1:511139/149‑1 (MQ=255)
                    ttACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACaaa                                                                                                                                <  1:299058/149‑1 (MQ=255)
                    ttACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACaaa                                                                                                                                <  1:412305/149‑1 (MQ=255)
                     tACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAAc                                                                                                                               <  3:177428/149‑1 (MQ=255)
                     tACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAAc                                                                                                                               >  2:687719/1‑149 (MQ=255)
                        cAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGt                                                                                                                            <  1:687719/149‑1 (MQ=255)
                            tCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCaa                                                                                                                        <  1:1075170/149‑1 (MQ=255)
                            tCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCaa                                                                                                                        >  2:202771/1‑149 (MQ=255)
                              aCGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATc                                                                                                                      >  2:281053/1‑149 (MQ=255)
                                            tGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCt                                                                                                        >  4:129009/1‑149 (MQ=255)
                                             gAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAAc                                                                                                                               <  2:792021/125‑1 (MQ=255)
                                             gAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAAc                                                                                                                               >  1:792021/1‑125 (MQ=255)
                                              aaGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTcc                                                                                                      >  2:414971/1‑149 (MQ=255)
                                                gATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAg                                                                                                    <  3:129009/149‑1 (MQ=255)
                                                gATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAg                                                                                                    <  1:778269/149‑1 (MQ=255)
                                                 aTTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAgg                                                                                                   >  4:878668/1‑149 (MQ=255)
                                                  ttttACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGc                                                                                                                                            <  4:1114817/107‑1 (MQ=255)
                                                  ttttACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGc                                                                                                                                            >  3:1114817/1‑107 (MQ=255)
                                                          cgcTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGa                                                                                          <  3:333377/149‑1 (MQ=255)
                                                            cTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAgc                                                                                        <  1:1055104/149‑1 (MQ=255)
                                                             ttAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTa                                                                                              >  1:656349/1‑142 (MQ=255)
                                                             ttAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTa                                                                                              <  2:656349/142‑1 (MQ=255)
                                                             ttAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAgcg                                                                                       >  2:1104620/1‑149 (MQ=255)
                                                                 cATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGcgccgc                                                                                   >  2:959463/1‑149 (MQ=255)
                                                                   tCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGcgccgccg                                                                                 >  2:224313/1‑149 (MQ=255)
                                                                   tCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGcgccgccg                                                                                 >  1:848990/1‑149 (MQ=255)
                                                                     cTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGt                                                                                                            <  4:560661/120‑1 (MQ=255)
                                                                     cTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGt                                                                                                            >  3:560661/1‑120 (MQ=255)
                                                                      tGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCgcag                                                                              >  2:81172/1‑149 (MQ=255)
                                                                         gcAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGcagcag                                                                           <  1:281053/149‑1 (MQ=255)
                                                                           aGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGgc                                                                         <  1:1000831/149‑1 (MQ=255)
                                                                            gCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGgcg                                                                        >  1:1149933/1‑149 (MQ=255)
                                                                              tttACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGcc                                                                                                                   <  2:254698/104‑1 (MQ=255)
                                                                              tttACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGcc                                                                                                                   >  1:254698/1‑104 (MQ=255)
                                                                                  cGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCaa                                                                  <  1:548196/149‑1 (MQ=255)
                                                                                         tGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACtt                                                           <  3:415806/149‑1 (MQ=255)
                                                                                          ggTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTg                                                          >  4:507325/1‑149 (MQ=255)
                                                                                           gtgtTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGt                                                         <  1:959463/149‑1 (MQ=255)
                                                                                             gtTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTaa                                                       <  1:849305/149‑1 (MQ=255)
                                                                                                 gCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAgc                                                                                        <  2:790150/112‑1 (MQ=255)
                                                                                                 gCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAgc                                                                                        >  1:790150/1‑112 (MQ=255)
                                                                                                 gCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCa                                                   >  4:255575/1‑149 (MQ=255)
                                                                                                   ccATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCa                                                                                                                                                  <  4:302333/52‑1 (MQ=255)
                                                                                                   ccATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCa                                                                                                                                                  >  3:302333/1‑52 (MQ=255)
                                                                                                   ccATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGa                                                 <  1:1104620/149‑1 (MQ=255)
                                                                                                   ccATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGa                                                 <  1:224313/149‑1 (MQ=255)
                                                                                                    cATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGAt                                                >  1:1055861/1‑149 (MQ=255)
                                                                                                          cTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAg                                          <  1:81172/149‑1 (MQ=255)
                                                                                                          cTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAg                                          >  3:3395/1‑149 (MQ=255)
                                                                                                           tATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTaa                                                       <  3:1007945/135‑1 (MQ=255)
                                                                                                           tATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTaa                                                       >  4:1007945/1‑135 (MQ=255)
                                                                                                           tATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGt                                         >  2:625056/1‑149 (MQ=255)
                                                                                                           tATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGt                                         >  2:426113/1‑149 (MQ=255)
                                                                                                                ccTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGaaaa                                    <  1:414971/149‑1 (MQ=255)
                                                                                                                 cTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGcgccgc                                                                                   >  3:930353/1‑101 (MQ=255)
                                                                                                                 cTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGcgccgc                                                                                   <  4:930353/101‑1 (MQ=255)
                                                                                                                   gCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGa                                 <  1:202771/149‑1 (MQ=255)
                                                                                                                      aTAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCt                              >  2:135711/1‑149 (MQ=255)
                                                                                                                        aaaaaTATCTCGAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCGAGCCAc                                                             >  3:754096/1‑116 (MQ=255)
                                                                                                                        aaaaaTATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCAc                                                             <  4:754096/116‑1 (MQ=255)
                                                                                                                         aaaaTATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGcc                           >  3:810540/1‑149 (MQ=255)
                                                                                                                           aaTATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGcc                                                    <  2:1091364/122‑1 (MQ=255)
                                                                                                                           aaTATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGcc                                                    >  1:1091364/1‑122 (MQ=255)
                                                                                                                             tatCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTc                       <  1:1066405/149‑1 (MQ=255)
                                                                                                                             tatCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTc                       <  3:507325/149‑1 (MQ=255)
                                                                                                                                 tcCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGCGAAAACGATCTGCCGCTCGTGc                   >  1:987051/1‑149 (MQ=255)
                                                                                                                                   cAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCgg                 >  2:552130/1‑149 (MQ=255)
                                                                                                                                     gATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGGCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGaa               >  1:216486/1‑149 (MQ=255)
                                                                                                                                      aTAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCa                                           >  3:51563/1‑120 (MQ=255)
                                                                                                                                      aTAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCa                                           <  4:51563/120‑1 (MQ=255)
                                                                                                                                         acgctgcctgAACAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGt                                         >  4:587405/4‑119 (MQ=255)
                                                                                                                                         acgctgcctgAACAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGt                                         <  3:587405/116‑1 (MQ=255)
                                                                                                                                         acgctgcctgAACAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAgcgc           >  1:666625/4‑149 (MQ=255)
                                                                                                                                           gctgcctgAACAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGaa                                      <  3:1025262/119‑1 (MQ=255)
                                                                                                                                           gctgcctgAACAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGaa                                      >  4:1025262/2‑120 (MQ=255)
                                                                                                                                           gctgcctgAACAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCaa         >  2:922266/2‑149 (MQ=255)
                                                                                                                                           gctgcctgAACAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCaa         <  1:922266/148‑1 (MQ=255)
                                                                                                                                            ctgcctgTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAg        >  4:182295/1‑149 (MQ=255)
                                                                                                                                            ctgcctgTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAg        <  3:182295/149‑1 (MQ=255)
                                                                                                                                                  gTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGaa  >  2:664596/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGAA  >  NC_000913/1948032‑1948326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: