Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,059,950 T→A 5.2% intergenic (+24/+14) asnU → / ← cbl tRNA‑Asn/ssuEADCB/tauABCD operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,059,9500TA 5.2% 399.6 / 8.1 111intergenic (+24/+14)asnU/cbltRNA‑Asn/ssuEADCB/tauABCD operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (56/49);  new base A (3/3);  total (59/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

TGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTCAGTC  >  NC_000913/2059808‑2060097
                                                                                                                                              |                                                                                                                                                   
tGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCggg                                                                                                                                               <  3:31943/149‑1 (MQ=255)
  aCAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGc                                                                                                                                                  <  2:735244/144‑1 (MQ=255)
  aCAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGAt                                                                                                                                             >  3:915103/1‑149 (MQ=255)
  aCAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGAt                                                                                                                                             >  3:847588/1‑149 (MQ=255)
  aCAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGAt                                                                                                                                             <  2:327496/149‑1 (MQ=255)
    aaaaGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGAttt                                                                                                                                           >  1:366787/1‑149 (MQ=255)
    aaaaGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGAttt                                                                                                                                           >  1:112359/1‑149 (MQ=255)
      aaGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAATCCCGCTATAAAAGCGGGCttttc                                                                                                                                         <  4:100905/149‑2 (MQ=21)
      aaGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGAttttt                                                                                                                                         <  4:548837/149‑1 (MQ=255)
                 ccGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGa                                                                                                                              <  3:56092/149‑1 (MQ=255)
                        gaagtgCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTATAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGCGTCCAGTCAGAGGTGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCat                                                                                                                       >  1:414406/4‑148 (MQ=255)
                        gATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACGGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATAGGTGATAATCaa                                                                                                                       >  2:331851/1‑149 (MQ=255)
                            tgCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTc                                                                                                                   <  4:905976/149‑1 (MQ=255)
                             gCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTtata                                                                                                                                                    >  4:852808/1‑115 (MQ=255)
                             gCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTcc                                                                                                                  <  1:559975/149‑1 (MQ=255)
                               cTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCctc                                                                                                                <  2:366787/149‑1 (MQ=255)
                               cTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCctc                                                                                                                >  2:1125047/1‑149 (MQ=255)
                                   acaacGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCa                                                                                                            >  1:22069/1‑149 (MQ=255)
                                        gATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCt                                                                                                       <  1:1125047/149‑1 (MQ=255)
                                        gATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACCGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCt                                                                                                       >  4:436972/1‑149 (MQ=255)
                                           tCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTc                                                                                                    <  4:915103/149‑1 (MQ=255)
                                             ctctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCGAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGa                                                                                                                              <  3:780551/121‑1 (MQ=255)
                                             ctctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGa                                                                                                                              >  4:780551/1‑121 (MQ=255)
                                             ctctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCctc                                                                                                                >  4:575090/1‑135 (MQ=255)
                                             ctctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCctc                                                                                                                <  3:575090/135‑1 (MQ=255)
                                                tGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGa                                                                                                                              <  4:917715/118‑1 (MQ=255)
                                                tGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGa                                                                                                                              >  3:917715/1‑118 (MQ=255)
                                                 gTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACGGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCAc                                                                                              >  2:326151/1‑149 (MQ=255)
                                                     ttCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCctc                                                                                                                >  2:131563/1‑127 (MQ=255)
                                                     ttCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCctc                                                                                                                <  1:131563/127‑1 (MQ=255)
                                                     ttCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGc                                                                                          >  1:128506/1‑149 (MQ=255)
                                                     ttCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGc                                                                                          >  3:452773/1‑149 (MQ=255)
                                                            ggTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTc                                                                                                                   >  2:471272/1‑117 (MQ=255)
                                                            ggTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTc                                                                                                                   <  1:471272/117‑1 (MQ=255)
                                                             gTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCa                                                                                                            <  4:1134224/123‑1 (MQ=255)
                                                             gTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCa                                                                                                            >  3:1134224/1‑123 (MQ=255)
                                                                gAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATc                                                                               >  3:476744/1‑149 (MQ=255)
                                                                gAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATc                                                                               >  2:848494/1‑149 (MQ=255)
                                                                   cggcggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTg                                                                                    >  3:502671/1‑141 (MQ=255)
                                                                   cggcggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTg                                                                                    <  4:502671/141‑1 (MQ=255)
                                                                         aCTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa                                                                      >  1:520568/1‑149 (MQ=255)
                                                                           tGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAATCCCGCTATAAAAGCGGGCttt                                                                                                                                           <  1:627913/78‑1 (MQ=14)
                                                                           tGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAATCCCGCTATAAAAGCGGGCttt                                                                                                                                           >  2:627913/1‑78 (MQ=14)
                                                                           tGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGata                                                                    >  2:620355/1‑147 (MQ=255)
                                                                           tGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACa                                                                    >  3:838171/1‑149 (MQ=255)
                                                                             ttAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATACTCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGt                                                                  >  1:149894/1‑149 (MQ=255)
                                                                              tAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTc                                                                 <  1:336963/149‑1 (MQ=255)
                                                                               aaTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCctct                                                                                                               >  3:420517/1‑102 (MQ=255)
                                                                               aaTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCctct                                                                                                               <  4:420517/102‑1 (MQ=255)
                                                                                aTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCt                                                               >  1:1121958/1‑149 (MQ=255)
                                                                                 tCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACt                                                                                                          >  4:607350/1‑105 (MQ=255)
                                                                                 tCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACt                                                                                                          <  3:607350/105‑1 (MQ=255)
                                                                                     tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCAtt                                                          <  3:436972/149‑1 (MQ=255)
                                                                                     tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCAtt                                                          <  3:948087/149‑1 (MQ=255)
                                                                                          cACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCtt                                                                                                              <  2:630915/92‑1 (MQ=255)
                                                                                          cACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCtt                                                                                                              >  1:630915/1‑92 (MQ=255)
                                                                                          cACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCa                                                            <  4:909471/142‑1 (MQ=255)
                                                                                          cACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCa                                                            <  4:833849/142‑1 (MQ=255)
                                                                                          cACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCa                                                            >  3:833849/1‑142 (MQ=255)
                                                                                          cACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCa                                                            >  3:909471/1‑142 (MQ=255)
                                                                                           aCTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGAGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAg                                                    >  1:740951/1‑149 (MQ=255)
                                                                                                ttCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCa                                               <  1:848494/149‑1 (MQ=255)
                                                                                                ttCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCa                                               <  4:476744/149‑1 (MQ=255)
                                                                                                      tCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgc                                         >  2:396635/1‑149 (MQ=255)
                                                                                                        cAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcgc                                       >  3:838632/1‑149 (MQ=255)
                                                                                                         aGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATc                                                                               <  2:190748/108‑1 (MQ=255)
                                                                                                         aGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATc                                                                               >  1:190748/1‑108 (MQ=255)
                                                                                                         aGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGcc                                      >  2:370672/1‑149 (MQ=255)
                                                                                                         aGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGcc                                      >  3:1048406/1‑149 (MQ=255)
                                                                                                          gTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAGGCGCCa                                     <  1:1066907/149‑1 (MQ=255)
                                                                                                           tCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACaaa                                                     <  1:322197/132‑1 (MQ=255)
                                                                                                            cAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGa                                   <  1:816716/149‑1 (MQ=255)
                                                                                                             agagGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGAc                                  >  3:365376/1‑149 (MQ=255)
                                                                                                                 gagCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAg                              <  1:370672/149‑1 (MQ=255)
                                                                                                                    ccAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTa                           >  4:320155/1‑149 (MQ=255)
                                                                                                                     cAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTAc                          >  4:1056135/1‑149 (MQ=255)
                                                                                                                       aaTTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGc                        <  2:1121958/149‑1 (MQ=255)
                                                                                                                           ccTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATACTCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGaa                    <  2:149894/149‑1 (MQ=255)
                                                                                                                           ccTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGaa                    <  1:396635/149‑1 (MQ=255)
                                                                                                                             tGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGaaa                                           <  1:42420/124‑1 (MQ=255)
                                                                                                                             tGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGaaa                                           >  2:42420/1‑124 (MQ=255)
                                                                                                                             tGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGt                  >  1:28855/1‑149 (MQ=255)
                                                                                                                              gAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGtt                 >  2:1140068/1‑149 (MQ=255)
                                                                                                                              gAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGtt                 >  1:191952/1‑149 (MQ=255)
                                                                                                                              aaaaaTCCCGCTATAAAAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcgc                                       >  4:1130166/2‑127 (MQ=255)
                                                                                                                              aaaaaTCCCGCTATAAAAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGtt                 >  4:794800/2‑149 (MQ=255)
                                                                                                                               aaaaGCCCGCTTCTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTg                >  3:683593/1‑149 (MQ=255)
                                                                                                                                 aaGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTc              <  2:128506/149‑1 (MQ=255)
                                                                                                                                    cccGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGaa                    >  3:799059/1‑140 (MQ=255)
                                                                                                                                    cccGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGaa                    <  4:799059/140‑1 (MQ=255)
                                                                                                                                    cccGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTc           >  1:781232/1‑149 (MQ=255)
                                                                                                                                    cccGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTc           >  1:800586/1‑149 (MQ=255)
                                                                                                                                    cccGCTTTTATAGCGGGATTTTTGCTATATATGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTc           >  1:642612/1‑149 (MQ=255)
                                                                                                                                      cGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACGAAGTTCCAGAAAGCGCCAGACATa                               >  4:457819/1‑127 (MQ=255)
                                                                                                                                      cGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGc         >  2:968461/1‑149 (MQ=255)
                                                                                                                                       gCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCt        <  4:683593/149‑1 (MQ=255)
                                                                                                                                       gCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCt        >  3:1042623/1‑149 (MQ=255)
                                                                                                                                            tataGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTCAGt   <  4:838171/149‑1 (MQ=255)
                                                                                                                                              taGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTa                                                         <  4:1116821/93‑1 (MQ=255)
                                                                                                                                              taGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTa                                                         >  3:1116821/1‑93 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
TGACAAAAGATTTTTCGCCGTTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTCAGTC  >  NC_000913/2059808‑2060097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: