Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,311,607 A→C 5.9% intergenic (‑73/+39) apbE ← / ← ompC putative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,311,6070AC 5.9% 298.7 / 10.3 85intergenic (‑73/+39)apbE/ompCputative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base A (44/36);  new base C (2/3);  total (46/39)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

Only 100 of 102 total aligned reads displayed.

TTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGG  >  NC_000913/2311460‑2311755
                                                                                                                                                   |                                                                                                                                                    
ttGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGaaaa                                                                                                                                                     >  2:435412/1‑149 (MQ=255)
  gtgGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGaaaaaa                                                                                                                                                   <  1:640023/149‑1 (MQ=255)
  gtgGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGaaaaaa                                                                                                                                                   <  1:56613/149‑1 (MQ=255)
   tgGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAg                                                                                                                                                  >  1:776812/1‑149 (MQ=255)
   tgGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAg                                                                                                                                                  <  3:706769/149‑1 (MQ=255)
     gTTTTTGATCGCAACCAACAAAGAAGAGCGCGGTAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAATGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAggg                                                                                                                                                >  4:901629/1‑149 (MQ=255)
      tttttGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGaaaaaa                                                                                                                                                   <  2:343642/145‑1 (MQ=255)
      tttttGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGaaaaaa                                                                                                                                                   >  1:343642/1‑145 (MQ=255)
           gATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGaaaaaa                                                                                                                                                   >  4:894312/1‑140 (MQ=255)
           gATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGaaaaaa                                                                                                                                                   <  3:894312/140‑1 (MQ=255)
           gATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCa                                                                                                                                          >  1:223585/1‑149 (MQ=255)
             tCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAgg                                                                                                                                        >  4:1044370/1‑149 (MQ=255)
               gCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGcc                                                                                                                                      <  2:330902/149‑1 (MQ=255)
                 aaccaacAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCt                                                                                                                                    <  4:332196/149‑1 (MQ=255)
                   ccaacAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAACAAAGGGCCTGCGGGCCCttt                                                                                                                                  >  2:472472/1‑149 (MQ=255)
                   ccaacAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCttt                                                                                                                                  >  4:393725/1‑149 (MQ=255)
                    caacaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTg                                                                                                                                 >  3:819683/1‑149 (MQ=255)
                     aacaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGt                                                                                                                                >  4:2738/1‑149 (MQ=255)
                      acaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAACAAAGGGCCTGCGGGCCCtttttt                                                                                                                               <  1:472472/149‑1 (MQ=255)
                      acaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAACAAAGGGCCTGCGGGCCCtttttt                                                                                                                               <  2:277560/149‑1 (MQ=255)
                      acaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGtt                                                                                                                               <  1:629093/149‑1 (MQ=255)
                         aagaagAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGa                                                                                                                            <  1:759486/149‑1 (MQ=255)
                                    ggCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAACAAAGGGCCTGCGGGCCCTTTTTTcat                                                                                                                            >  2:275292/1‑136 (MQ=255)
                                    ggCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAACAAAGGGCCTGCGGGCCCTTTTTTcat                                                                                                                            <  1:275292/138‑3 (MQ=255)
                                    ggCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCgag                                                                                                                 <  3:2738/149‑1 (MQ=255)
                                       agccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGAtt                                                                                                              <  3:393725/149‑1 (MQ=255)
                                              agTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTg                                                                                                       <  3:1044370/149‑1 (MQ=255)
                                                   cACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTaaa                                                                                                  <  3:90130/149‑1 (MQ=255)
                                                    aCGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAAc                                                                                                 >  1:322023/1‑149 (MQ=255)
                                                    aCGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAAc                                                                                                 >  2:610810/1‑149 (MQ=255)
                                                        gggTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGa                                                                                             <  4:819683/149‑1 (MQ=255)
                                                        gggTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGa                                                                                             >  3:181013/1‑149 (MQ=255)
                                                           tAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGAccc                                                                                          <  4:995336/149‑1 (MQ=255)
                                                                 ttATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCt                                                                                    >  2:792349/1‑149 (MQ=255)
                                                                     ttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGa                                                                                >  1:122152/1‑149 (MQ=255)
                                                                         aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGtt                                                                            >  3:477004/1‑149 (MQ=255)
                                                                         aTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGtt                                                                            >  1:672932/1‑149 (MQ=255)
                                                                          tACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTa                                                                           >  3:188366/1‑149 (MQ=255)
                                                                          tACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTa                                                                           >  2:844656/1‑149 (MQ=255)
                                                                           aCGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTAt                                                                          >  3:280527/1‑149 (MQ=255)
                                                                             gTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCa                                                                        >  2:664860/1‑149 (MQ=255)
                                                                                 ttATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAgtgt                                                                    <  2:122152/149‑1 (MQ=255)
                                                                                 ttATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAgtgt                                                                    <  1:792349/149‑1 (MQ=255)
                                                                                  tATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGtt                                                                   <  2:776812/149‑1 (MQ=255)
                                                                                      cTCATGCGAACGGTCGCAAGAGTACAACAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATg                                                               >  2:102128/1‑149 (MQ=255)
                                                                                       tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                                                              >  2:1025772/1‑149 (MQ=255)
                                                                                       tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                                                              >  3:1122069/1‑149 (MQ=255)
                                                                                       tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                                                              >  3:317001/1‑149 (MQ=255)
                                                                                       tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                                                              >  2:311553/1‑149 (MQ=255)
                                                                                         aTGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTgg                                                                                                      <  2:952148/107‑1 (MQ=255)
                                                                                         aTGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTgg                                                                                                      >  1:952148/1‑107 (MQ=255)
                                                                                         aTGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGTTACGATGTTATCAGTGTTGATGCCa                                                            >  4:432206/1‑149 (MQ=255)
                                                                                         aTGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCa                                                            <  4:280527/149‑1 (MQ=255)
                                                                                          tGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAg                                                           >  1:547758/1‑149 (MQ=255)
                                                                                                  gTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGa                                                   <  4:477004/149‑1 (MQ=255)
                                                                                                     gCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTg                                                <  4:1122069/149‑1 (MQ=255)
                                                                                                       aaGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAg                                                                       <  3:1116075/124‑1 (MQ=255)
                                                                                                       aaGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAg                                                                       >  4:1116075/1‑124 (MQ=255)
                                                                                                       aaGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGaa                                              <  1:610810/149‑1 (MQ=255)
                                                                                                        agagTACACCAAAAAACTGAGTTAGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGACCTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAAc                                             <  1:102128/149‑1 (MQ=255)
                                                                                                            tACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGt                                         >  1:286430/1‑149 (MQ=255)
                                                                                                             acacCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGtt                                        <  2:547758/149‑1 (MQ=255)
                                                                                                               acCAAAAAACTGAGTTTGTAGGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGtt                                        >  2:33746/1‑147 (MQ=255)
                                                                                                               acCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGtt                                        <  1:33746/147‑1 (MQ=255)
                                                                                                                ccAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtc                                     >  3:561373/1‑149 (MQ=255)
                                                                                                                 cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGaa                                                                                                          <  3:786217/79‑1 (MQ=255)
                                                                                                                 cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCa                                                                        >  4:414648/1‑113 (MQ=255)
                                                                                                                 cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCa                                                                        <  3:414648/113‑1 (MQ=255)
                                                                                                                 cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcg                                    >  1:1051111/1‑149 (MQ=255)
                                                                                                                 cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcg                                    >  1:623564/1‑149 (MQ=255)
                                                                                                                 cAAAAAACTGAATTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGGTCGATATCAATCGAGATTAGaa                                                                                                          >  4:786217/1‑79 (MQ=255)
                                                                                                                   aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgtc                                  >  2:654047/1‑149 (MQ=255)
                                                                                                                    aaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTcc                                 <  3:901629/149‑1 (MQ=255)
                                                                                                                          gAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                                                              <  4:1114864/114‑1 (MQ=255)
                                                                                                                          gAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                                                              >  3:1114864/1‑114 (MQ=255)
                                                                                                                          gAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAgg                           <  1:844656/149‑1 (MQ=255)
                                                                                                                            gTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCg                                                                                                                             >  2:630993/1‑49 (MQ=255)
                                                                                                                            gTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCg                                                                                                                             <  1:630993/49‑1 (MQ=255)
                                                                                                                             tttGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgtc                                  >  2:984850/1‑139 (MQ=255)
                                                                                                                             tttGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgtc                                  <  1:984850/139‑1 (MQ=255)
                                                                                                                                gTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAtt                     <  3:432206/149‑1 (MQ=255)
                                                                                                                                gTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAtt                     <  4:181013/149‑1 (MQ=255)
                                                                                                                                 tACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAttt                    >  2:599773/1‑149 (MQ=255)
                                                                                                                                  aCGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAtttt                   <  1:311553/149‑1 (MQ=255)
                                                                                                                                     cTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                <  2:223585/149‑1 (MQ=255)
                                                                                                                                     cTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                <  1:435412/149‑1 (MQ=255)
                                                                                                                                      tGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAg               >  4:695211/1‑149 (MQ=255)
                                                                                                                                       gAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                <  4:320337/147‑1 (MQ=255)
                                                                                                                                       gAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                >  3:320337/1‑147 (MQ=255)
                                                                                                                                             aaTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt        >  1:346323/1‑149 (MQ=255)
                                                                                                                                               tGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAg      >  4:1028535/1‑149 (MQ=255)
                                                                                                                                                 aaaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGGCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                <  1:750606/137‑1 (MQ=255)
                                                                                                                                                  aaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAtttt                   <  3:533517/133‑1 (MQ=255)
                                                                                                                                                  aaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAtttt                   >  4:533517/1‑133 (MQ=255)
                                                                                                                                                  aaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTg   >  4:421350/1‑149 (MQ=255)
                                                                                                                                                  aaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTg   <  3:695211/149‑1 (MQ=255)
                                                                                                                                                   aaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtc                                     <  1:617507/114‑1 (MQ=255)
                                                                                                                                                   aaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtc                                     >  2:617507/1‑114 (MQ=255)
                                                                                                                                                   aaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTgg  >  2:309146/1‑149 (MQ=255)
                                                                                                                                                   aaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTgg  <  1:654047/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGG  >  NC_000913/2311460‑2311755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: