Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,425,968 T→G 5.6% intergenic (‑52/+38) hisQ ← / ← hisJ histidine ABC transporter permease/histidine ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,425,9680TG 5.6% 442.4 / 14.7 125intergenic (‑52/+38)hisQ/hisJhistidine ABC transporter permease/histidine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (56/62);  new base G (3/4);  total (59/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 129 total aligned reads displayed.

TTAAACCGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGTT  >  NC_000913/2425820‑2426113
                                                                                                                                                    |                                                                                                                                                 
ttAAACCGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGt                                                                                                                                                   >  4:782575/1‑149 (MQ=255)
      cGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCg                                                                                                                                             >  3:232627/1‑149 (MQ=255)
      cGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCg                                                                                                                                             >  3:849210/1‑149 (MQ=255)
      cGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCg                                                                                                                                             >  4:173512/1‑149 (MQ=255)
        atgatTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGaa                                                                                                                                           >  2:1139263/1‑149 (MQ=255)
         tgatTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAg                                                                                                                                          <  3:264427/149‑1 (MQ=255)
         tgatTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAg                                                                                                                                          <  1:57050/149‑1 (MQ=255)
                    aGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctt                                                                                                                               <  1:1047250/149‑1 (MQ=255)
                      cACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttc                                                                                                                             <  3:282820/149‑1 (MQ=255)
                      cACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttc                                                                                                                             <  1:1139263/149‑1 (MQ=255)
                      cACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttc                                                                                                                             >  2:1111891/1‑149 (MQ=255)
                       aCTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttct                                                                                                                            <  3:415092/149‑1 (MQ=255)
                         tACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTGCCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttcttt                                                                                                                          <  3:537156/149‑1 (MQ=255)
                            agagCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTTCGGGTAACCGGAGGGa                                                                                                                                       <  4:671669/133‑1 (MQ=255)
                            agagCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTTCGGGTAACCGGAGGGa                                                                                                                                       >  1:754779/1‑133 (MQ=255)
                            agagCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCgg                                                                                                                                   >  3:812597/1‑137 (MQ=255)
                            agagCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCgg                                                                                                                                   <  4:812597/137‑1 (MQ=255)
                              agCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCg                                                                                                                                    >  4:147055/1‑134 (MQ=255)
                              agCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCg                                                                                                                                    <  3:147055/134‑1 (MQ=255)
                               gCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGa                                                                                                                    >  4:285535/1‑149 (MQ=255)
                                 tGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATg                                                                                                                  >  2:755106/1‑149 (MQ=255)
                                 tGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATg                                                                                                                  <  4:542234/149‑1 (MQ=255)
                                 tGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATg                                                                                                                  >  4:375601/1‑149 (MQ=255)
                                  gATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATgg                                                                                                                 <  3:285535/149‑1 (MQ=255)
                                     aGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGAGAGCGGGCTTTCTTTCACCGATGGCGa                                                                                                              <  3:811799/149‑1 (MQ=255)
                                       ccAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCAc                                                                                                                       <  1:391621/138‑1 (MQ=255)
                                       ccAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCAc                                                                                                                       <  1:13450/138‑1 (MQ=255)
                                       ccAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCAc                                                                                                                       >  2:391621/1‑138 (MQ=255)
                                       ccAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCAc                                                                                                                       >  2:13450/1‑138 (MQ=255)
                                       ccAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGAtt                                                                                                            >  4:583186/1‑149 (MQ=255)
                                        cAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTa                                                                                                           <  3:782575/149‑1 (MQ=255)
                                             ccAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGccac                                                                                                      <  3:261388/149‑1 (MQ=255)
                                             ccAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGccac                                                                                                      <  4:689735/149‑1 (MQ=255)
                                                gCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTTCGGGTAACCGGAGGGa                                                                                                                                       >  1:906207/1‑113 (MQ=255)
                                                gCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTTCGGGTAACCGGAGGGa                                                                                                                                       <  2:906207/113‑1 (MQ=255)
                                                    gACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGa                                                                                                                                       <  4:699460/109‑1 (MQ=255)
                                                    gACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTAGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGa                                                                                                                                       >  3:699460/1‑109 (MQ=255)
                                                     aCGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACa                                                                                              <  1:749470/149‑1 (MQ=255)
                                                       gAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATc                                                                                            >  1:499929/1‑149 (MQ=255)
                                                        aGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCa                                                                                           >  4:92635/1‑149 (MQ=255)
                                                             aCCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATc                                                                                      <  1:334941/149‑1 (MQ=255)
                                                              cccTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCg                                                                                     <  1:90005/149‑1 (MQ=255)
                                                                cTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGAGTAGCCACCATAAACATCAAAATCGaa                                                                                   <  4:728863/149‑1 (MQ=255)
                                                                   tAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTa                                                                                >  2:475732/1‑149 (MQ=255)
                                                                   tAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTa                                                                                >  3:943214/1‑149 (MQ=255)
                                                                      aaTAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGaa                                                                                   <  3:1058489/143‑1 (MQ=255)
                                                                      aaTAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGaa                                                                                   >  4:1058489/1‑143 (MQ=255)
                                                                            acCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGaa                                                                                                                                           >  2:584266/1‑81 (MQ=255)
                                                                            acCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGaa                                                                                                                                           <  1:584266/81‑1 (MQ=255)
                                                                              cTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTa                                                                     >  1:324632/1‑149 (MQ=255)
                                                                                gAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAAt                                                                   >  4:480466/1‑149 (MQ=255)
                                                                                    aCCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTtct                                                               >  4:434728/1‑149 (MQ=255)
                                                                                     cccATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTtctc                                                              <  2:700173/149‑1 (MQ=255)
                                                                                     cccATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTtctc                                                              <  3:667621/149‑1 (MQ=255)
                                                                                         tACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAGGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTaa                                                          <  1:1111891/149‑1 (MQ=255)
                                                                                          acaacaTGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAg                                                         <  1:1065122/149‑1 (MQ=255)
                                                                                           caacaTGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGt                                                        >  4:938431/1‑149 (MQ=255)
                                                                                            aacaTGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTa                                                       >  3:145021/1‑149 (MQ=255)
                                                                                               aTGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCg                                                    >  2:305717/1‑149 (MQ=255)
                                                                                                tgctgcCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGt                                                   <  3:173512/149‑1 (MQ=255)
                                                                                                tgctgcCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGt                                                   >  4:380057/1‑149 (MQ=255)
                                                                                                  ctgcCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCa                                                 <  3:480466/149‑1 (MQ=255)
                                                                                                  ctgcCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCa                                                 <  3:944376/149‑1 (MQ=255)
                                                                                                    gcCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAgc                                               >  3:101586/1‑149 (MQ=255)
                                                                                                     cctgtcctgtCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAgcg                                              >  2:215284/1‑149 (MQ=255)
                                                                                                     cctgtcatgcCGTATGTGGTGACTTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGTGGGCTTTCTTTCGCCGATGGCGATTCGCCATCCTAAACATCAAAATCCAAGTACTTTTTCGGTAATTTCTCGGAAGTACCGTCAgcg                                              >  2:173200/1‑149 (MQ=255)
                                                                                                      ctgtcctgtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAgcgc                                             <  1:1063188/149‑1 (MQ=255)
                                                                                                           ctgtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCAtt                                        >  1:286878/1‑149 (MQ=255)
                                                                                                            tgtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCAttt                                       >  1:881842/1‑149 (MQ=255)
                                                                                                              tcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGCTTAGCCACCATAAACATCAAAATCGCAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCg                                     <  1:173200/149‑1 (MQ=255)
                                                                                                                gtgtgtGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGc                                   >  2:199547/1‑149 (MQ=255)
                                                                                                                   tgtgGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCaaa                                <  3:434728/149‑1 (MQ=255)
                                                                                                                     tggtgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCAttt                                       >  1:727137/1‑140 (MQ=255)
                                                                                                                     tggtgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCAttt                                       <  2:727137/140‑1 (MQ=255)
                                                                                                                       gtgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTAcc                                                     <  4:798872/124‑1 (MQ=255)
                                                                                                                       gtgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTc                                      >  1:1080788/1‑139 (MQ=255)
                                                                                                                       gtgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTc                                      <  2:1080788/139‑1 (MQ=255)
                                                                                                                       gtgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCt                            >  2:356505/1‑149 (MQ=255)
                                                                                                                       gtgAATTACAGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTAcc                                                     >  3:798872/1‑124 (MQ=255)
                                                                                                                        tgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCtt                           <  2:762796/149‑1 (MQ=255)
                                                                                                                         gAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCttt                          <  3:375601/149‑1 (MQ=255)
                                                                                                                         gAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCttt                          <  2:881842/149‑1 (MQ=255)
                                                                                                                         gAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCttt                          <  1:356505/149‑1 (MQ=255)
                                                                                                                         gAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCttt                          <  4:145021/149‑1 (MQ=255)
                                                                                                                         gAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCttt                          <  4:232627/149‑1 (MQ=255)
                                                                                                                             tACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTc                      <  1:199547/149‑1 (MQ=255)
                                                                                                                                cGCCTCTTGTTTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCATCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATATCGGCAAAGGCTTTGTTCAGt                   >  3:706232/1‑149 (MQ=255)
                                                                                                                                 gCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTg                  >  4:1061283/1‑149 (MQ=255)
                                                                                                                                 gCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTg                  >  4:923256/1‑149 (MQ=255)
                                                                                                                                   ctctTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCt                >  4:1059194/1‑149 (MQ=255)
                                                                                                                                        gTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTcgcg           >  3:305347/1‑149 (MQ=255)
                                                                                                                                             ccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCttt                          <  3:910172/129‑1 (MQ=255)
                                                                                                                                             cctccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGtt      >  3:317037/1‑149 (MQ=255)
                                                                                                                                              ctccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTc     >  4:401723/1‑149 (MQ=255)
                                                                                                                                                ccGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAAtt                                                                  >  3:1088682/1‑86 (MQ=255)
                                                                                                                                                ccGGTTACCCGAAGGGAGCGAGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGt   <  3:583186/149‑1 (MQ=255)
                                                                                                                                                 cGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGtt  <  1:475732/149‑1 (MQ=255)
                                                                                                                                                 cGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGtt  <  4:320678/149‑1 (MQ=255)
                                                                                                                                                    ttACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTAcc                                                     <  4:124874/95‑1 (MQ=255)
                                                                                                                                                    ttACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTAcc                                                     >  3:124874/1‑95 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TTAAACCGATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGTT  >  NC_000913/2425820‑2426113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: