BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,581,765	T→C	6.5%	D338G (GAC→GGC)	ypfG ←	DUF1176 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,581,765	0	T	C	6.5%	339.0 / 8.7	106	D338G (GAC→GGC)	ypfG	DUF1176 family protein
Reads supporting (aligned to +/- strand): ref base T (55/44); new base C (3/4); total (58/48)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.10e-01

Only 100 of 106 total aligned reads displayed.

GCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTC  >  NC_000913/2581618‑2581910

gCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTc                                                                                                                                                  >  2:763679/1‑149 (MQ=255)

  tGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTg                                                                                                                                                <  1:727007/149‑1 (MQ=255)

       gTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCa                                                                                                                                           <  3:710160/149‑1 (MQ=255)

       gTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGACGCCTGGCCCa                                                                                                                                           <  3:89206/149‑1 (MQ=255)

        tGAAAGGTGCCTGATGGTGATTTCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGCCTGGCCCAt                                                                                                                                          <  1:986370/149‑1 (MQ=255)

        tGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCAt                                                                                                                                          >  1:178759/1‑149 (MQ=255)

                gCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTa                                                                                                                                  <  1:763679/149‑1 (MQ=255)

                 ccTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTAt                                                                                                                                 <  1:1086864/149‑1 (MQ=255)

                  cTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATc                                                                                                                                <  3:160732/149‑1 (MQ=255)

                  cTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATc                                                                                                                                <  2:178759/149‑1 (MQ=255)

                   tGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCg                                                                                                                               >  3:592930/1‑149 (MQ=255)

                                   atgctgattgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCATCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGAGCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGAGGGTTCTgcgg                                                                                                               <  4:104795/149‑1 (MQ=255)

                                     gctgattgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTgcggcg                                                                                                             >  1:1118492/1‑149 (MQ=255)

                                      ctgattgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGt                                                                                                            >  2:153455/1‑149 (MQ=255)

                                       tgattgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTa                                                                                                           <  3:654858/149‑1 (MQ=255)

                                       tgattgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTa                                                                                                           >  2:410972/1‑149 (MQ=255)

                                       tgattgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTa                                                                                                           >  4:233425/1‑149 (MQ=255)

                                        gattgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTaa                                                                                                          >  4:671095/1‑149 (MQ=255)

                                          ttgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAgg                                                                                                                           <  3:366713/130‑1 (MQ=255)

                                          ttgccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAgg                                                                                                                           >  4:366713/1‑130 (MQ=255)

                                            gccggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCa                                                                                                      <  2:500126/149‑1 (MQ=255)

                                               ggatgcGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCa                                                                                                   >  3:789201/1‑149 (MQ=255)

                                                     gACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGaa                                                                                             >  1:656430/1‑149 (MQ=255)

                                                       cGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGaaaa                                                                                           <  4:199940/149‑1 (MQ=255)

                                                        gCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTCACGCACCAGGCGAAAAc                                                                                          >  4:121595/1‑149 (MQ=255)

                                                        gCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGaaa                                                                                            >  1:1053759/1‑147 (MQ=255)

                                                        gCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGaaa                                                                                            <  2:1053759/147‑1 (MQ=255)

                                                           gACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGTCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCATGCGAAAACGCt                                                                                       <  4:786129/149‑1 (MQ=255)

                                                             cgcgTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGa                                                                                     <  2:656430/149‑1 (MQ=255)

                                                              gcgTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGAc                                                                                    <  3:233425/149‑1 (MQ=255)

                                                               cgTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGAcc                                                                                   <  3:85976/149‑1 (MQ=255)

                                                                  cTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTc                                                                                <  1:410972/149‑1 (MQ=255)

                                                                      tCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAg                                                                            >  3:55550/1‑149 (MQ=255)

                                                                         ggCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAgcgc                                                                         >  3:231565/1‑149 (MQ=255)

                                                                                   gTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCt                                                               >  3:750931/1‑149 (MQ=255)

                                                                                    tCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTgc                                                                                                                 >  3:758661/1‑98 (MQ=255)

                                                                                    tCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTgc                                                                                                                 <  4:758661/98‑1 (MQ=255)

                                                                                        aTCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATg                                                          <  4:592930/149‑1 (MQ=255)

                                                                                            ggCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGAc                                                                                    >  4:822730/1‑119 (MQ=255)

                                                                                            ggCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGAc                                                                                    <  3:822730/119‑1 (MQ=255)

                                                                                            ggCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCcac                                                      >  2:92593/1‑149 (MQ=255)

                                                                                             gCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCcaca                                                     >  1:803692/1‑149 (MQ=255)

                                                                                             gCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCcaca                                                     >  3:963520/1‑149 (MQ=255)

                                                                                                tACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATc                                                  <  3:1024852/149‑1 (MQ=255)

                                                                                                tACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATc                                                  >  1:210296/1‑149 (MQ=255)

                                                                                                  cATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGc                                                <  1:92593/149‑1 (MQ=255)

                                                                                                     gTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGc                                                         >  2:222617/1‑137 (MQ=255)

                                                                                                     gTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGc                                                         <  1:222617/137‑1 (MQ=255)

                                                                                                     gTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTa                                             >  1:138366/1‑149 (MQ=255)

                                                                                                       cccGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCt                                                                                       >  1:405589/1‑105 (MQ=255)

                                                                                                       cccGCCATTTTGTTTAACGCGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCt                                                                                       <  2:405589/105‑1 (MQ=255)

                                                                                                         cGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATcc                                         >  1:1011604/1‑149 (MQ=255)

                                                                                                         cGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATcc                                         >  1:815930/1‑149 (MQ=255)

                                                                                                           ccATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCaaa                                                                             <  1:590416/111‑1 (MQ=255)

                                                                                                           ccATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCaaa                                                                             >  2:590416/1‑111 (MQ=255)

                                                                                                             aTTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCgcgc                                     >  4:1076107/1‑149 (MQ=255)

                                                                                                             aTTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCgcgc                                     <  4:750931/149‑1 (MQ=255)

                                                                                                              ttttGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTc                                                                                <  4:736686/105‑1 (MQ=255)

                                                                                                              ttttGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTc                                                                                >  3:736686/1‑105 (MQ=255)

                                                                                                              ttttGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCca                                                       <  3:1120567/130‑1 (MQ=255)

                                                                                                              ttttGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCca                                                       >  4:1120567/1‑130 (MQ=255)

                                                                                                                  gTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAgc                                                                           <  3:173402/106‑1 (MQ=255)

                                                                                                                  gTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAgc                                                                           >  4:173402/1‑106 (MQ=255)

                                                                                                                  gTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGc                                                >  2:760235/1‑133 (MQ=255)

                                                                                                                  gTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGc                                                <  1:760235/133‑1 (MQ=255)

                                                                                                                  gTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGc                                                >  4:1037648/1‑133 (MQ=255)

                                                                                                                  gTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGc                                                <  3:1037648/133‑1 (MQ=255)

                                                                                                                   tttAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTc                               >  1:109886/1‑149 (MQ=255)

                                                                                                                   tttAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTc                               <  2:1011604/149‑1 (MQ=255)

                                                                                                                   tttAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTc                               <  1:153455/149‑1 (MQ=255)

                                                                                                                     tAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGc                             <  2:210296/149‑1 (MQ=255)

                                                                                                                      aaCGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCTCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCca                                                       <  4:939632/122‑1 (MQ=255)

                                                                                                                      aaCGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCca                                                       >  3:939632/1‑122 (MQ=255)

                                                                                                                      aaCGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCt                            >  1:1020155/1‑149 (MQ=255)

                                                                                                                        cGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTaa                          <  2:1118492/149‑1 (MQ=255)

                                                                                                                         gggTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAg                         >  3:507849/1‑149 (MQ=255)

                                                                                                                          ggTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAgg                        >  4:384940/1‑149 (MQ=255)

                                                                                                                               tCCACAGCGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCAcc                   >  2:815527/1‑149 (MQ=255)

                                                                                                                               tCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCAcc                   <  4:789201/149‑1 (MQ=255)

                                                                                                                               tCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCAcc                   >  3:212400/1‑149 (MQ=255)

                                                                                                                                ccACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCa                  >  2:279574/1‑149 (MQ=255)

                                                                                                                                 cacaACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTg                                                              >  1:204389/1‑104 (MQ=255)

                                                                                                                                 cacaACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAg                 >  3:887952/1‑149 (MQ=255)

                                                                                                                                 cacaACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAg                 >  2:785262/1‑149 (MQ=255)

                                                                                                                                  acaACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGc                                                <  2:145700/117‑1 (MQ=255)

                                                                                                                                  acaACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGc                                                >  1:145700/1‑117 (MQ=255)

                                                                                                                                  acaACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGt                >  4:870358/1‑149 (MQ=255)

                                                                                                                                       atGGGCCAGACGCCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGACAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATcc                                         <  3:99031/118‑1 (MQ=255)

                                                                                                                                       atGGGCCAGACGCCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATcc                                         <  1:1101141/118‑1 (MQ=255)

                                                                                                                                       atGGGCCAGACGCCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATcc                                         >  2:1101141/2‑119 (MQ=255)

                                                                                                                                       atGGGCCAGACGCCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCAcg           >  1:719675/2‑149 (MQ=255)

                                                                                                                                        tGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCa                                                                       <  3:866360/88‑1 (MQ=255)

                                                                                                                                        tGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCa                                                                       >  4:866360/1‑88 (MQ=255)

                                                                                                                                        tGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCAcgc          <  1:312227/149‑1 (MQ=255)

                                                                                                                                           gCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGAt       >  4:590072/1‑149 (MQ=255)

                                                                                                                                           gCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGAt       >  4:352057/1‑149 (MQ=255)

                                                                                                                                            ccAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGAtt      >  2:26861/1‑149 (MQ=255)

                                                                                                                                             cAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGAttt     <  2:803692/149‑1 (MQ=255)

                                                                                                                                              aGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTtc    >  4:374654/1‑149 (MQ=255)

                                                                                                                                                gCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTtctc  >  4:209616/1‑149 (MQ=255)

GCTGGGAGTGAAAGGTGCCTGATGGTGATTGCCGGATGCTGATTGCCGGATGCGACGCTGACGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTC  >  NC_000913/2581618‑2581910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`