Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,219,179 T→A 5.8% intergenic (‑103/‑315) aer ← / → patA fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component/putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,219,1790TA 5.8% 306.0 / 6.7 86intergenic (‑103/‑315)aer/patAfused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component/putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (37/44);  new base A (3/2);  total (40/46)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATACCCGA  >  NC_000913/3219037‑3219326
                                                                                                                                              |                                                                                                                                                   
gCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGa                                                                                                                                               <  3:532282/149‑1 (MQ=255)
   gggTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGAttt                                                                                                                                            >  2:760034/1‑149 (MQ=255)
     gTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTaa                                                                                                                                          >  4:626830/1‑149 (MQ=255)
      tATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTaaa                                                                                                                                         <  1:422404/149‑1 (MQ=255)
           tGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACaa                                                                                                                                    <  4:551176/149‑1 (MQ=255)
             cTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAAt                                                                                                                                  <  2:769323/149‑1 (MQ=255)
               gggTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCt                                                                                                                                >  4:18635/1‑149 (MQ=255)
                    aCATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTc                                                                                                                           <  2:687885/149‑1 (MQ=255)
                      aTACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCt                                                                                                                         <  4:1029993/149‑1 (MQ=255)
                           gATGAGAAGACATGGTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGAtt                                                                                                                    >  3:81519/1‑149 (MQ=255)
                                gaagaCATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAAATCGCAAATTGCGAtc                                                                                                                                             <  1:247587/119‑2 (MQ=255)
                                gaagaCATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAAATCGCAAATTGCGAtc                                                                                                                                             >  2:247587/1‑118 (MQ=255)
                                    aCATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTGCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTATCCGAGTAATTTGATTTAGGTCGCAATTTGCGATTTAAGCAGAAATCTAATTCCTTGATTTAAAATACt                                                                                                           <  2:508004/149‑1 (MQ=255)
                                     cATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACtt                                                                                                          <  4:525700/149‑1 (MQ=255)
                                      aTGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACttt                                                                                                         <  1:1114880/149‑1 (MQ=255)
                                      aTGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACttt                                                                                                         >  1:810262/1‑149 (MQ=255)
                                      aTGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTCGATTTAAAATACttt                                                                                                         >  2:585543/1‑149 (MQ=255)
                                        gTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCa                                                                                                       >  2:139411/1‑149 (MQ=255)
                                        gTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCa                                                                                                       >  1:382932/1‑149 (MQ=255)
                                        gTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCa                                                                                                       <  1:139411/149‑1 (MQ=255)
                                          tCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCAct                                                                                                     <  2:810262/149‑1 (MQ=255)
                                           cgcgTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCt                                                                                                                         <  2:508059/128‑1 (MQ=255)
                                           cgcgTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCt                                                                                                                         >  1:508059/1‑128 (MQ=255)
                                              gTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGt                                                                                                 <  4:559963/149‑1 (MQ=255)
                                               tCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGtt                                                                                                >  3:923473/1‑149 (MQ=255)
                                               tCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAAATTGCGATCTaaa                                                                                                                                         <  3:1067821/108‑1 (MQ=255)
                                               tCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAAATTGCGATCTaaa                                                                                                                                         >  4:1067821/1‑108 (MQ=255)
                                                 cTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTAc                                                                                              <  4:865401/149‑1 (MQ=255)
                                                 cTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTAc                                                                                              <  2:806643/149‑1 (MQ=255)
                                                  tgtgCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACt                                                                                             <  2:382932/149‑1 (MQ=255)
                                                        ggTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGa                                                                                       >  3:297077/1‑149 (MQ=255)
                                                        ggTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGa                                                                                       >  1:69602/1‑149 (MQ=255)
                                                         gTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGaa                                                                                      >  2:462156/1‑149 (MQ=255)
                                                           tataTGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGaaaa                                                                                    <  4:81519/149‑1 (MQ=255)
                                                            atatGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCGATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAAc                                                                                   <  2:69602/149‑1 (MQ=255)
                                                            atatGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAAc                                                                                   <  1:760034/149‑1 (MQ=255)
                                                               tGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGtt                                                                                >  2:803076/1‑149 (MQ=255)
                                                               tGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGtt                                                                                >  1:586664/1‑149 (MQ=255)
                                                                 gttgttAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTaa                                                                              <  3:822810/149‑1 (MQ=255)
                                                                 gttgttAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTaa                                                                              <  3:1134053/149‑1 (MQ=255)
                                                                   tgttAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTCGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAAtt                                                                            <  1:585543/149‑1 (MQ=255)
                                                                       aaCTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGaa                                                                                      >  1:201325/1‑135 (MQ=255)
                                                                       aaCTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGaa                                                                                      <  2:201325/135‑1 (MQ=255)
                                                                          ttcttGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACtata                                                                                          >  1:976658/1‑128 (MQ=255)
                                                                          ttcttGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACtata                                                                                          <  2:976658/128‑1 (MQ=255)
                                                                          ttcttGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGc                                                                     <  1:23232/149‑1 (MQ=255)
                                                                                   gagTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATc                                                            >  1:1130005/1‑149 (MQ=255)
                                                                                     gTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAg                                                          >  3:57221/1‑149 (MQ=255)
                                                                                     gTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAg                                                          <  3:18635/149‑1 (MQ=255)
                                                                                         aTGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCaa                                                      >  2:526536/1‑149 (MQ=255)
                                                                                                  ccGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAg                                                          >  1:775186/1‑136 (MQ=255)
                                                                                                  ccGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAg                                                          <  2:775186/136‑1 (MQ=255)
                                                                                                    gCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTg                                           >  2:58384/1‑149 (MQ=255)
                                                                                                         ggTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCa                                                           <  4:669916/128‑1 (MQ=255)
                                                                                                         ggTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCa                                                           >  3:669916/1‑128 (MQ=255)
                                                                                                         ggTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAAc                                      <  3:468540/149‑1 (MQ=255)
                                                                                                           ttATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCa                                                                                                       <  3:865057/82‑1 (MQ=255)
                                                                                                           ttATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCa                                                                                                       >  4:865057/1‑82 (MQ=255)
                                                                                                               cTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCAt                                <  4:57221/149‑1 (MQ=255)
                                                                                                                tttAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCAtt                               >  1:1117472/1‑149 (MQ=255)
                                                                                                                tttAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCAtt                               >  3:456994/1‑149 (MQ=255)
                                                                                                                tttAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCAtt                               >  1:466032/1‑149 (MQ=255)
                                                                                                                tttAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCAtt                               >  2:187815/1‑149 (MQ=255)
                                                                                                                      cGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCTTGAAAACGTGCAttt                              <  4:1095797/144‑1 (MQ=255)
                                                                                                                      cGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCAttt                              >  3:1095797/1‑144 (MQ=255)
                                                                                                                       gATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCgcag                        <  2:1117472/149‑1 (MQ=255)
                                                                                                                        aTTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCgcagc                       <  4:297077/149‑1 (MQ=255)
                                                                                                                           aaTTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAat                    <  3:900388/149‑1 (MQ=255)
                                                                                                                            aTTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAatc                   >  3:554535/1‑149 (MQ=255)
                                                                                                                             tttGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATa                                                                                                             <  4:376238/58‑1 (MQ=255)
                                                                                                                             tttGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATa                                                                                                             >  3:376238/1‑58 (MQ=255)
                                                                                                                             tttGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCa                                                           >  3:917039/1‑108 (MQ=255)
                                                                                                                             tttGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCa                                                           <  4:917039/108‑1 (MQ=255)
                                                                                                                             tttGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCAttt                              <  2:224654/137‑1 (MQ=255)
                                                                                                                             tttGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCAttt                              >  1:224654/1‑137 (MQ=255)
                                                                                                                             tttGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAatca                  >  2:51717/1‑149 (MQ=255)
                                                                                                                             tttGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAatca                  >  2:993292/1‑149 (MQ=255)
                                                                                                                              ttGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAatcat                 <  1:462156/149‑1 (MQ=255)
                                                                                                                                 aTTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCaa              <  1:58384/149‑1 (MQ=255)
                                                                                                                                  tttAAATCGCAAATTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCaaa             >  1:859502/1‑149 (MQ=255)
                                                                                                                                    tAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATc           <  1:51717/149‑1 (MQ=255)
                                                                                                                                    tAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATc           <  4:923473/149‑1 (MQ=255)
                                                                                                                                        tCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAattatt                                                 <  3:834782/107‑1 (MQ=255)
                                                                                                                                        tCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAattatt                                                 >  4:834782/1‑107 (MQ=255)
                                                                                                                                           cAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATAccc    >  1:581409/1‑149 (MQ=255)
                                                                                                                                             aTTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATACCCGa  <  1:803076/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
GCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATACCCGA  >  NC_000913/3219037‑3219326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: