Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,486,517 G→A 94.1% G133D (GGC→GAC)  crp → cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,486,5170GA94.1% 335.8 / 8.5 102G133D (GGC→GAC) crpcAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base A (47/49);  total (50/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.02e-01

Only 100 of 102 total aligned reads displayed.

GGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCG  >  NC_000913/3486376‑3486661
                                                                                                                                             |                                                                                                                                                
gggTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTg                                                                                                                                           >  1:897384/1‑149 (MQ=255)
      gTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTc                                                                                                                                     <  1:1105274/149‑1 (MQ=255)
      gTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTc                                                                                                                                     <  4:542786/149‑1 (MQ=255)
       tGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTcc                                                                                                                                    <  3:557384/149‑1 (MQ=255)
        gCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCt                                                                                                                                   <  3:126379/149‑1 (MQ=255)
         cGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTc                                                                                                                                  <  1:411572/149‑1 (MQ=255)
            aaaCCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGAc                                                                                                                               <  1:150589/149‑1 (MQ=255)
            aaaCCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGAc                                                                                                                               <  4:1066302/149‑1 (MQ=255)
             aaCCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACg                                                                                                                              >  2:244492/1‑149 (MQ=255)
                cGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGa                                                                                                                           >  2:1010192/1‑149 (MQ=255)
                 gCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGAc                                                                                                                          <  2:334733/149‑1 (MQ=255)
                          gTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCAt                                                                                                                 >  4:338231/1‑149 (MQ=255)
                          gTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCAt                                                                                                                 <  2:416701/149‑1 (MQ=255)
                           tGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCAtt                                                                                                                <  2:183844/149‑1 (MQ=255)
                              cTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGca                                                                                                             <  3:747650/149‑1 (MQ=255)
                               tGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTc                                                                                                                                     <  3:1140320/124‑1 (MQ=255)
                               tGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTc                                                                                                                                     >  4:1140320/1‑124 (MQ=255)
                               tGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGcac                                                                                                            >  4:492867/1‑149 (MQ=255)
                               tGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGcac                                                                                                            >  3:625004/1‑149 (MQ=255)
                                gAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGcaca                                                                                                           <  2:212905/149‑1 (MQ=255)
                                    tttCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGAct                                                                                                       >  1:1041007/1‑149 (MQ=255)
                                        gTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTctgc                                                                                                   <  2:1041007/149‑1 (MQ=255)
                                         tACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTctgct                                                                                                  >  2:966822/1‑149 (MQ=255)
                                         tACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTctgct                                                                                                  <  3:338231/149‑1 (MQ=255)
                                            aaaaaaTTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCa                                                                                                                  >  3:427382/1‑130 (MQ=255)
                                            aaaaaaTTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCa                                                                                                                  <  4:427382/130‑1 (MQ=255)
                                            aaaaaaTTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGaa                                                                                               >  3:413791/1‑149 (MQ=255)
                                            aaaaaaTTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGaa                                                                                               >  3:395013/1‑149 (MQ=255)
                                            aaaaaaTTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGaa                                                                                               >  2:121127/1‑149 (MQ=255)
                                            aaaaaaTTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGaa                                                                                               >  1:771878/1‑149 (MQ=255)
                                            aaaaaaTTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTATGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGaa                                                                                               >  4:940238/1‑149 (MQ=255)
                                                 aTTTCGCCAATTGATTTAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTgg                                                                                          >  2:755629/1‑149 (MQ=255)
                                                        cAATTGATTGAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACaa                                                                                                                                               <  4:698283/89‑1 (MQ=255)
                                                        cAATTGATTGAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACaa                                                                                                                                               >  3:698283/1‑89 (MQ=255)
                                                         aaTTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAaacaa                                                                                  <  4:625004/149‑1 (MQ=255)
                                                              aTTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGa                                                                             <  3:152687/149‑1 (MQ=255)
                                                              aTTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGa                                                                             >  4:632185/1‑149 (MQ=255)
                                                                  aGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCt                                                                         <  4:362948/149‑1 (MQ=255)
                                                                  aGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCt                                                                         >  3:1105243/1‑149 (MQ=255)
                                                                  aGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACAAGACGCt                                                                         >  3:663765/1‑149 (MQ=255)
                                                                   ggTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGcc                                                                                                                     >  4:1050423/1‑104 (MQ=255)
                                                                   ggTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGcc                                                                                                                     <  3:1050423/104‑1 (MQ=255)
                                                                   ggTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTa                                                                        >  3:141220/1‑149 (MQ=255)
                                                                    gTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTAt                                                                       <  1:971449/149‑1 (MQ=255)
                                                                    gTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTAt                                                                       <  3:349012/149‑1 (MQ=255)
                                                                      aaaCCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGa                                                                     <  3:191402/149‑1 (MQ=255)
                                                                          ccGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTa                                                                        <  4:35262/142‑1 (MQ=255)
                                                                          ccGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTa                                                                        >  3:35262/1‑142 (MQ=255)
                                                                          ccGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCa                                                                 <  2:1149894/149‑1 (MQ=255)
                                                                          ccGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCa                                                                 >  4:655259/1‑149 (MQ=255)
                                                                          ccGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCa                                                                 >  2:13359/1‑149 (MQ=255)
                                                                          ccGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCa                                                                 >  3:273435/1‑149 (MQ=255)
                                                                          ccGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCa                                                                 >  1:928851/1‑149 (MQ=255)
                                                                                  tCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACg                                                         >  1:286936/1‑149 (MQ=255)
                                                                                       tGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATg                                                    >  4:437478/1‑149 (MQ=255)
                                                                                        gCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGc                                                   <  1:879553/149‑1 (MQ=255)
                                                                                          gTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCaa                                                 >  2:157559/1‑149 (MQ=255)
                                                                                           tttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCaaa                                                <  1:90370/149‑1 (MQ=255)
                                                                                           tttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCaaa                                                >  4:68757/1‑149 (MQ=255)
                                                                                            ttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAAt                                               <  3:940238/149‑1 (MQ=255)
                                                                                              gTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGa                                                           >  2:808451/1‑135 (MQ=255)
                                                                                              gTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGa                                                           <  1:808451/135‑1 (MQ=255)
                                                                                               tCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGa                                                                             >  2:128346/1‑116 (MQ=255)
                                                                                               tCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGa                                                                             <  1:128346/116‑1 (MQ=255)
                                                                                                 tGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCaa                                            >  4:485166/1‑147 (MQ=255)
                                                                                                 tGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCaa                                            <  3:485166/147‑1 (MQ=255)
                                                                                                 tGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGcaca                                                                                                           >  3:767485/1‑84 (MQ=255)
                                                                                                 tGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGcaca                                                                                                           <  4:767485/84‑1 (MQ=255)
                                                                                                 tGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCaaaa                                          >  3:343777/1‑149 (MQ=255)
                                                                                                 tGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCaaaa                                          <  4:425644/149‑1 (MQ=255)
                                                                                                 tGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCaaaa                                          <  2:286936/149‑1 (MQ=255)
                                                                                                 tGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCaaaa                                          <  3:979122/149‑1 (MQ=255)
                                                                                                  gCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAAt                                         <  3:492867/149‑1 (MQ=255)
                                                                                                    acaGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGTCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTa                                       >  1:759637/1‑149 (MQ=255)
                                                                                                    acaGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTa                                       >  3:352157/1‑149 (MQ=255)
                                                                                                     caGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTAc                                      <  3:1099010/149‑1 (MQ=255)
                                                                                                      aGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTAcc                                     <  4:273435/149‑1 (MQ=255)
                                                                                                             gcgTCGTCTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAgg                              >  1:1045872/1‑149 (MQ=255)
                                                                                                                 cgtcTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAAt                          <  1:966822/149‑1 (MQ=255)
                                                                                                                   tcTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCa                                >  3:999644/1‑141 (MQ=255)
                                                                                                                   tcTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTg                        <  1:157559/149‑1 (MQ=255)
                                                                                                                   tcTGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTg                        >  1:1065877/1‑149 (MQ=255)
                                                                                                                     tGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGt                      >  3:156878/1‑149 (MQ=255)
                                                                                                                     tGCAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCCATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGt                      <  1:121127/149‑1 (MQ=255)
                                                                                                                       cAAGTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCa                    <  4:395013/149‑1 (MQ=255)
                                                                                                                          gTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGa                  <  1:652956/148‑1 (MQ=255)
                                                                                                                          gTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGa                  >  2:652956/1‑148 (MQ=255)
                                                                                                                          gTCACTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGAt                 >  1:188599/1‑149 (MQ=255)
                                                                                                                             aCTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGTCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGt              <  2:759637/149‑1 (MQ=255)
                                                                                                                             aCTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGt              >  3:939248/1‑149 (MQ=255)
                                                                                                                             aCTTCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGt              <  3:68757/149‑1 (MQ=255)
                                                                                                                               ttCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCg            >  1:1144111/1‑149 (MQ=255)
                                                                                                                               ttCAGAGAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCg            >  1:468713/1‑149 (MQ=255)
                                                                                                                                  agagAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCt         <  1:83971/149‑1 (MQ=255)
                                                                                                                                  agagAAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAAACAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCt         <  4:939248/149‑1 (MQ=255)
                                                                                                                                   gagaAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTg        <  2:771878/149‑1 (MQ=255)
                                                                                                                                    agaAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGt       >  4:896990/1‑149 (MQ=255)
                                                                                                                                     gaAAGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGtt      >  4:436118/1‑149 (MQ=255)
                                                                                                                                        aGTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTtctc   <  2:188599/149‑1 (MQ=255)
                                                                                                                                         gTGGACAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCg  <  2:1144111/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                
GGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCG  >  NC_000913/3486376‑3486661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: