Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,722,300 G→T 5.6% intergenic (+195/+28) sokA → / ← glyS pseudogene, sRNA antisense regulator blocking mokA/hokA translation/glycine tRNA synthetase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,722,3000GT 5.6% 694.4 / 20.8 195intergenic (+195/+28)sokA/glySpseudogene, sRNA antisense regulator blocking mokA/hokA translation/glycine tRNA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (110/74);  new base T (6/5);  total (116/79)
Fisher's exact test for biased strand distribution p-value = 7.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.64e-01

Only 100 of 198 total aligned reads displayed.

CCCGTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGAAA  >  NC_000913/3722152‑3722448
                                                                                                                                                    |                                                                                                                                                    
cccGTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAg                                                                                                                                                      <  2:238473/149‑1 (MQ=255)
 ccGTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGa                                                                                                                                                     <  1:604152/149‑1 (MQ=255)
   gTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATg                                                                                                                                                   <  4:519430/149‑1 (MQ=255)
                cTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTtaa                                                                                                                                      <  4:506251/149‑1 (MQ=255)
                      aaGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACgg                                                                                                                                <  2:828385/149‑1 (MQ=255)
                               tcttctCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTtattat                                                                                                                       >  4:763655/1‑149 (MQ=255)
                                    tcCTTGATATAATCCTAAAAAAATCTAATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCATGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCaa                                                                                                                  >  4:974293/1‑149 (MQ=255)
                                      cTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGc                                                                                                                                                 <  2:772358/116‑1 (MQ=255)
                                      cTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGc                                                                                                                                                 >  1:772358/1‑116 (MQ=255)
                                      cTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGttatt                                                                                                                         <  3:16493/140‑1 (MQ=255)
                                      cTTGATATAATCCTAAAAAAATCTCATAAAATTAACATATGAGATAATCTTTATTCCGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACa                                                                                                                >  4:603722/1‑149 (MQ=255)
                                                tCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTAtt                                                                                                                                         >  3:828129/1‑114 (MQ=255)
                                                 ccTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATcc                                                                                                     >  1:286061/1‑149 (MQ=255)
                                                  cTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCg                                                                                                    >  1:1003291/1‑149 (MQ=255)
                                                      aaaaaTCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAAc                                                                                                >  4:459246/1‑149 (MQ=255)
                                                       aaaaTCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACa                                                                                                                >  2:548148/1‑132 (MQ=255)
                                                       aaaaTCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACa                                                                                                                <  1:548148/132‑1 (MQ=255)
                                                           tctcATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCATCTGGCAGGCtt                                                                                                                                           <  3:625214/101‑2 (MQ=255)
                                                           tctcATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCATCTGGCAGGCtt                                                                                                                                           >  4:625214/1‑100 (MQ=255)
                                                               aTAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTAtt                                                                                                                                         <  2:1127566/99‑1 (MQ=255)
                                                                  aaaTTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGAt                                                                                                                                                    <  3:398574/85‑1 (MQ=255)
                                                                  aaaTTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGAt                                                                                                                                                    >  4:398574/1‑85 (MQ=255)
                                                                   aaTTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGt                                                                                   <  3:1061630/149‑1 (MQ=255)
                                                                    aTTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGtt                                                                                  >  3:287953/1‑149 (MQ=255)
                                                                     ttAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGttatt                                                                                                                         >  1:839638/1‑109 (MQ=255)
                                                                     ttAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAAc                                                                                      >  1:213704/1‑144 (MQ=255)
                                                                        atatatGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATc                                                                                                      >  1:751395/1‑125 (MQ=255)
                                                                                gaTAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTtct                                                                      >  1:397099/1‑149 (MQ=255)
                                                                                gaTAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTtct                                                                      >  3:774553/1‑149 (MQ=255)
                                                                                 aTAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAata                                                                                                        >  2:752853/1‑114 (MQ=255)
                                                                                   aaTCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCa                                                                   <  4:332208/149‑1 (MQ=255)
                                                                                   aaTCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCa                                                                   <  3:974293/149‑1 (MQ=255)
                                                                                    aTCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAg                                                                  >  4:1144996/1‑149 (MQ=255)
                                                                                     tCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGc                                                                 >  4:886069/1‑149 (MQ=255)
                                                                                       tttATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAAcgcg                                                                                             <  4:579962/119‑1 (MQ=255)
                                                                                       tttATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAAcgcg                                                                                             >  3:579962/1‑119 (MQ=255)
                                                                                       tttATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTcg                                                                                <  3:649827/132‑1 (MQ=255)
                                                                                       tttATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTcg                                                                                >  4:649827/1‑132 (MQ=255)
                                                                                       tttATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCAt                                                               >  3:1136021/1‑149 (MQ=255)
                                                                                          aTTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTcgc                                                                               >  1:16898/1‑130 (MQ=255)
                                                                                          aTTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTcgc                                                                               <  2:16898/130‑1 (MQ=255)
                                                                                              atcagAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAACATGGTCAGa                                                        >  4:220581/3‑149 (MQ=255)
                                                                                              agcagAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAg                                                                                                                                               >  2:1121564/1‑62 (MQ=255)
                                                                                              agcagAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAg                                                                                                                                               <  1:1121564/62‑1 (MQ=255)
                                                                                              agcagAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATg                                                              >  4:792272/1‑143 (MQ=255)
                                                                                              agcagAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATg                                                              <  3:792272/143‑1 (MQ=255)
                                                                                              agcagAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGAc                                                       >  2:149302/1‑149 (MQ=255)
                                                                                                 agaagaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACgg                                                     >  1:180065/1‑149 (MQ=255)
                                                                                                 agaagaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACgg                                                     >  1:15105/1‑149 (MQ=255)
                                                                                                  gaagaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTtc                                                                       >  3:744352/1‑130 (MQ=255)
                                                                                                  gaagaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTtc                                                                       <  4:744352/130‑1 (MQ=255)
                                                                                                  gaagaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGt                                                    <  2:1003291/149‑1 (MQ=255)
                                                                                                   aagaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTattatt                                                                                                                      >  4:1078542/1‑82 (MQ=255)
                                                                                                   aagaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGtt                                                   <  3:220581/149‑1 (MQ=255)
                                                                                                     gaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGa                                                 >  4:716835/1‑149 (MQ=255)
                                                                                                          ttaAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACgcgc                                                                                            >  2:612078/1‑101 (MQ=255)
                                                                                                          ttaAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGtt                                                                                  >  4:212671/1‑111 (MQ=255)
                                                                                                          ttaAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGtt                                                                                  <  3:212671/111‑1 (MQ=255)
                                                                                                          ttaAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGc                                            >  4:232908/1‑149 (MQ=255)
                                                                                                           taAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCa                                           >  1:933693/1‑149 (MQ=255)
                                                                                                             aaGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATgg                                                                                                                                                  >  1:487060/1‑44 (MQ=255)
                                                                                                             aaGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATc                                                                                                      <  4:92966/88‑1 (MQ=255)
                                                                                                             aaGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTcc                                                                    >  1:1037920/1‑122 (MQ=255)
                                                                                                               ggTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTc                                       >  1:721475/1‑149 (MQ=255)
                                                                                                                gTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCt                                      <  4:774553/149‑1 (MQ=255)
                                                                                                                gTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCt                                      <  2:286061/149‑1 (MQ=255)
                                                                                                                  tGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCttt                                    <  2:397099/149‑1 (MQ=255)
                                                                                                                    cTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGt                                  <  2:729072/149‑1 (MQ=255)
                                                                                                                     tGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGtc                                 >  2:571390/1‑149 (MQ=255)
                                                                                                                        attattTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGtcatc                              >  2:471175/1‑149 (MQ=255)
                                                                                                                         ttattTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGtcatca                             >  1:1150029/1‑149 (MQ=255)
                                                                                                                           attTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGt                                                    <  2:519537/124‑1 (MQ=255)
                                                                                                                           attTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGt                                                    >  1:519537/1‑124 (MQ=255)
                                                                                                                             ttAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCa                         >  2:952239/1‑149 (MQ=255)
                                                                                                                              tAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCAt                        <  3:603722/149‑1 (MQ=255)
                                                                                                                                cgttattaaataGCCTGCCATCTGGCAGGCTTTTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGa                      <  3:497190/142‑1 (MQ=39)
                                                                                                                                   aTAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGa                      <  2:136532/146‑1 (MQ=255)
                                                                                                                                    tAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTcc                                                                    >  3:509723/1‑99 (MQ=255)
                                                                                                                                    attaaataGCCTGCCATCTGGCAGGCTTTTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCAt                  >  4:815970/8‑149 (MQ=255)
                                                                                                                                     aaaaaaaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTtctc                                                                     >  3:939739/1‑97 (MQ=255)
                                                                                                                                     aaaaaaaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATc                 >  3:900014/1‑149 (MQ=255)
                                                                                                                                       aaaaaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGa                                                        <  4:195826/108‑1 (MQ=255)
                                                                                                                                       aaaaaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGa                                                        <  2:583994/108‑1 (MQ=255)
                                                                                                                                       aaaaaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGa                                                        >  1:583994/1‑108 (MQ=255)
                                                                                                                                       aaaaaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCAc               >  3:996086/1‑149 (MQ=255)
                                                                                                                                          aaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTc                                       <  4:592678/122‑1 (MQ=255)
                                                                                                                                          aaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTc                                       >  3:592678/1‑122 (MQ=255)
                                                                                                                                          aaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGt                                  >  4:104326/1‑127 (MQ=255)
                                                                                                                                          aaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGt                                  <  3:104326/127‑1 (MQ=255)
                                                                                                                                           aGCCTGCCATCTGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCttt                                    <  2:34632/124‑1 (MQ=255)
                                                                                                                                           aGCCTGCCATCTGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCttt                                    >  1:34632/1‑124 (MQ=255)
                                                                                                                                              cTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTcc                                                                    <  3:477812/89‑1 (MQ=255)
                                                                                                                                               tGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGa                                                        <  4:482911/100‑1 (MQ=255)
                                                                                                                                               tGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCtt                                     >  1:626347/1‑119 (MQ=255)
                                                                                                                                               tGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCga       >  3:331661/1‑149 (MQ=255)
                                                                                                                                                gCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCgaa      <  3:202505/149‑1 (MQ=255)
                                                                                                                                                gCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCgaa      >  4:845475/1‑149 (MQ=255)
                                                                                                                                                gCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCgaa      >  1:145975/1‑149 (MQ=255)
                                                                                                                                                   aGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCgaagaa   >  1:963619/1‑149 (MQ=255)
                                                                                                                                                    gATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGaaa  <  2:998786/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCCGTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGAAA  >  NC_000913/3722152‑3722448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: