Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,886,756 G→A 5.0% intergenic (+34/‑72) yidC → / → mnmE membrane protein insertase/tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,886,7560GA 5.0% 269.6 / 2.1 79intergenic (+34/‑72)yidC/mnmEmembrane protein insertase/tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase
Reads supporting (aligned to +/- strand):  ref base G (38/37);  new base A (2/2);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

AGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCATCTCC  >  NC_000913/3886608‑3886902
                                                                                                                                                    |                                                                                                                                                  
aGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAg                                                                                                                                                    <  3:749767/149‑1 (MQ=255)
 ggTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGt                                                                                                                                                   <  2:999836/149‑1 (MQ=255)
 ggTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGt                                                                                                                                                   <  1:20424/149‑1 (MQ=255)
  gTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGtt                                                                                                                                                  <  2:621765/149‑1 (MQ=255)
  gTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGtt                                                                                                                                                  <  2:1049413/149‑1 (MQ=255)
    cTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGa                                                                                                                                                <  2:361817/149‑1 (MQ=255)
    cTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGa                                                                                                                                                <  1:582552/149‑1 (MQ=255)
             tACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAGACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                       <  3:58746/149‑1 (MQ=255)
             tACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                       <  3:200493/149‑1 (MQ=255)
                tataTCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttc                                                                                                                                    >  4:203400/1‑149 (MQ=255)
                  tatCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttctt                                                                                                                                  >  4:253204/1‑149 (MQ=255)
                    tCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttctttt                                                                                                                                >  4:180589/1‑149 (MQ=255)
                    tCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttctttt                                                                                                                                >  1:638978/1‑149 (MQ=255)
                     cGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttcttttc                                                                                                                               <  1:520966/149‑1 (MQ=255)
                       tCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAg                                                                                                                                                    >  3:539596/1‑126 (MQ=255)
                       tCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAg                                                                                                                                                    <  4:539596/126‑1 (MQ=255)
                       tCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGt                                                                                                                             >  4:485574/1‑149 (MQ=255)
                       tCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGt                                                                                                                             <  3:253204/149‑1 (MQ=255)
                       tCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAACTGACCGccc                                                                                                                                          <  3:235428/136‑2 (MQ=255)
                       tCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAACTGACCGccc                                                                                                                                          >  4:235428/1‑135 (MQ=255)
                          gCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAgg                                                                                                                          <  3:1002579/149‑1 (MQ=255)
                          gCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAgg                                                                                                                          >  4:1002579/1‑149 (MQ=255)
                            aaCCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                       <  1:82353/134‑1 (MQ=255)
                            aaCCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttt                                                                                                                                       >  2:82353/1‑134 (MQ=255)
                            aaCCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGc                                                                                                                        >  2:41074/1‑149 (MQ=255)
                             aCCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCttttt                                                                                                                                      <  1:91819/134‑1 (MQ=255)
                             aCCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCttttt                                                                                                                                      >  2:91819/1‑134 (MQ=255)
                               cTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGa                                                                                                                     <  3:191701/149‑1 (MQ=255)
                                tGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAg                                                                                                                                                    <  3:336768/117‑1 (MQ=255)
                                tGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAg                                                                                                                                                    >  4:336768/1‑117 (MQ=255)
                                    aaCCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGc                                                                                                                <  1:41074/149‑1 (MQ=255)
                                       cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGt                                                                                                                             <  1:119098/133‑1 (MQ=255)
                                       cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGt                                                                                                                             >  2:119098/1‑133 (MQ=255)
                                       cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGAt                                                                                                                    >  2:417346/1‑142 (MQ=255)
                                       cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGAt                                                                                                                    <  1:417346/142‑1 (MQ=255)
                                       cATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCAcc                                                                                                             >  2:309238/1‑149 (MQ=255)
                                             tCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCAc                                                                                                              >  3:926438/1‑142 (MQ=255)
                                             tCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCAc                                                                                                              <  4:926438/142‑1 (MQ=255)
                                               agcagcagcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATcc                                                                                                     <  4:372334/149‑1 (MQ=255)
                                                gcagcagcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTCGACCGCCTTTTTTCTTTTCGTAGGGCGGATa                                                                                                                   >  3:491464/1‑134 (MQ=255)
                                                gcagcagcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTCGACCGCCTTTTTTCTTTTCGTAGGGCGGATa                                                                                                                   <  4:491464/134‑1 (MQ=255)
                                                    cagcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttttt                                                                                                                                     >  1:338832/1‑112 (MQ=255)
                                                    cagcTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtttttt                                                                                                                                     <  2:338832/112‑1 (MQ=255)
                                                         gATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACaaa                                                                                           <  3:485574/149‑1 (MQ=255)
                                                          aTTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAg                                                                                          >  3:970846/1‑149 (MQ=255)
                                                              aCCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGtt                                                                                                                                                  >  1:538567/1‑89 (MQ=255)
                                                              aCCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAAc                                                                                      <  3:203400/149‑1 (MQ=255)
                                                              aCCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAAGAAGGGCGGTCAGtt                                                                                                                                                  <  2:538567/89‑1 (MQ=255)
                                                               ccGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACa                                                                                     <  1:1049594/149‑1 (MQ=255)
                                                                    gTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAc                                                                                <  1:309238/149‑1 (MQ=255)
                                                                     tCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAca                                                                               >  4:559407/1‑149 (MQ=255)
                                                                       tGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAcatc                                                                             >  1:1148737/1‑149 (MQ=255)
                                                                                   gCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAAt                                                                 >  1:775428/1‑149 (MQ=255)
                                                                                     cTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGAc                                                                                                                                               >  2:16602/1‑69 (MQ=255)
                                                                                     cTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGAc                                                                                                                                               <  1:16602/69‑1 (MQ=255)
                                                                                     cTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGa                                                               >  1:285842/1‑149 (MQ=255)
                                                                                       gCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAACGaca                                                             <  3:180589/149‑1 (MQ=255)
                                                                                         aTAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACt                                                           <  3:559407/149‑1 (MQ=255)
                                                                                           aGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTAt                                                         <  3:528256/149‑1 (MQ=255)
                                                                                            gCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATc                                                        <  4:970846/149‑1 (MQ=255)
                                                                                               gcgAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATaa                                                                  >  1:475561/1‑136 (MQ=255)
                                                                                               gcgAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATaa                                                                  <  2:475561/136‑1 (MQ=255)
                                                                                                    aagaaAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCa                                                >  2:28420/1‑149 (MQ=255)
                                                                                                      gaaAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAgg                                              >  4:954122/1‑149 (MQ=255)
                                                                                                      gaaAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAgg                                              >  1:813597/1‑149 (MQ=255)
                                                                                                      gaaAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAgg                                              >  2:95219/1‑149 (MQ=255)
                                                                                                      gaaAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAgg                                              >  2:309118/1‑149 (MQ=255)
                                                                                                        aaaaaTCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGcc                                            >  1:1134196/1‑149 (MQ=255)
                                                                                                        aaaaaTCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAAATCATGAGCGATAATGACACTATCGTAGCCCAGGcc                                            >  1:47441/1‑149 (MQ=255)
                                                                                                            aTCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGc                                        >  3:833601/1‑149 (MQ=255)
                                                                                                                  aTTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCgg                                  >  2:1010498/1‑149 (MQ=255)
                                                                                                                        tGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACa                                                                                     <  4:772644/92‑1 (MQ=255)
                                                                                                                        tGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACa                                                                                     >  3:772644/1‑92 (MQ=255)
                                                                                                                        tGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTg                            >  4:669704/1‑149 (MQ=255)
                                                                                                                            ttttCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTggcgg                        >  3:1101490/1‑149 (MQ=255)
                                                                                                                                cGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGtt                    <  4:833601/149‑1 (MQ=255)
                                                                                                                                 gCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTg                   <  2:47441/149‑1 (MQ=255)
                                                                                                                                   tAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGc                 >  3:1006697/1‑149 (MQ=255)
                                                                                                                                      aaTAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATc              <  2:813597/149‑1 (MQ=255)
                                                                                                                                           aggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGtt                    <  4:505618/137‑1 (MQ=255)
                                                                                                                                           aggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGtt                    >  3:505618/2‑138 (MQ=255)
                                                                                                                                            ggCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTgcgc        >  4:270696/1‑149 (MQ=255)
                                                                                                                                               ggTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCAtc     >  4:584797/1‑149 (MQ=255)
                                                                                                                                                  cAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCATCTcc  <  2:285842/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGGTCTGGTGCTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCATCTCC  >  NC_000913/3886608‑3886902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: