Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,906,826	T→C	5.9%	intergenic (‑259/+27)	bglG ← / ← phoU	transcriptional antiterminator of the bgl operon/negative regulator of PhoR/PhoB two‑component regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,906,826	0	T	C	5.9%	221.9 / 3.9	68	intergenic (‑259/+27)	bglG/phoU	transcriptional antiterminator of the bgl operon/negative regulator of PhoR/PhoB two‑component regulator
Reads supporting (aligned to +/- strand):  ref base T (42/22);  new base C (2/2);  total (44/24)
Fisher's exact test for biased strand distribution p-value = 6.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.15e-01

ATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGT  >  NC_000913/3906688‑3906968                                                                                                                                           |                                                                                                                                               aTCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTg                                                                                                                                      >  3:75375/1‑149 (MQ=255)    cAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGa                                                                                                                                   <  1:120533/149‑1 (MQ=255)    cAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGa                                                                                                                                   >  2:120533/1‑149 (MQ=255)                    tttaattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTaa                                                                                                                                             <  4:161300/123‑1 (MQ=255)                    tttaattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTaa                                                                                                                                             >  3:161300/1‑123 (MQ=255)                    tttaattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAatta                                                                                                                   >  4:727665/1‑149 (MQ=255)                     ttaattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAattat                                                                                                                  <  4:75375/149‑1 (MQ=255)                                     aaaaTAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGt                                                                                                              <  2:630103/137‑1 (MQ=255)                                     aaaaTAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGt                                                                                                              >  1:630103/1‑137 (MQ=255)                                       aaTAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTcccc                                                                                                >  4:979831/1‑149 (MQ=255)                                        aTAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCg                                                                                               >  1:849870/1‑149 (MQ=255)                                           gCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCca                                                                                            <  2:543746/149‑1 (MQ=255)                                           gCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCca                                                                                            >  2:116287/1‑149 (MQ=255)                                               tGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCcagcag                                                                                        >  2:841236/1‑149 (MQ=255)                                                     aTTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTAtt                                                                                                                                       >  3:370339/1‑96 (MQ=255)                                                     aTTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTAtt                                                                                                                                       <  4:370339/96‑1 (MQ=255)                                                         aTAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATc                                                                                  <  2:597693/145‑1 (MQ=255)                                                         aTAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATc                                                                                  >  1:597693/1‑145 (MQ=255)                                                         aTAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGc                                                                              >  4:85375/1‑149 (MQ=255)                                                          tAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGc                                                                              <  1:513679/148‑1 (MQ=255)                                                          tAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGc                                                                              >  2:513679/1‑148 (MQ=255)                                                            aaaaTATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCa                                                                           >  2:946564/1‑149 (MQ=255)                                                                   gACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCAc                                                                    >  1:250901/1‑149 (MQ=255)                                                                     ccATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCg                                                                  >  4:29701/1‑149 (MQ=255)                                                                       aTGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAACTTAATTATTGGGa                                                                                                                                   >  1:721449/1‑82 (MQ=38)                                                                       aTGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAACTTAATTATTGGGa                                                                                                                                   <  2:721449/82‑1 (MQ=38)                                                                         gCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGt                                                              <  1:946564/149‑1 (MQ=255)                                                                           tCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGa                                                            <  3:979831/149‑1 (MQ=255)                                                                             gCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACg                                                          >  2:393130/1‑149 (MQ=255)                                                                                   attaACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCACAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATc                                                    >  3:489655/1‑149 (MQ=255)                                                                                    ttaACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGc                                                                              >  3:936190/1‑122 (MQ=255)                                                                                    ttaACATTGTGTAACTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGc                                                                              <  4:936190/122‑1 (MQ=255)                                                                                       aCTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGAATTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGc                                                <  1:841236/149‑1 (MQ=255)                                                                                        cTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGcc                                               >  1:404836/1‑149 (MQ=255)                                                                                        cTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGcc                                               >  4:679887/1‑149 (MQ=255)                                                                                              gtAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCa                                         >  3:1129200/1‑149 (MQ=255)                                                                                               tAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCAc                                        <  3:727665/149‑1 (MQ=255)                                                                                                aaTTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACg                                       <  2:849870/149‑1 (MQ=255)                                                                                                  ttttAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCAc                                        >  4:591707/1‑146 (MQ=255)                                                                                                  ttttAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCAc                                        <  3:591707/146‑1 (MQ=255)                                                                                                   tttAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCCGTCCCTTCACgtag                                    <  3:85375/149‑1 (MQ=255)                                                                                                    ttAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACgtagt                                   <  4:1129200/149‑1 (MQ=255)                                                                                                          aTTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTagaaga                             <  2:404836/149‑1 (MQ=255)                                                                                                          aTTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTagaaga                             >  2:1027918/1‑149 (MQ=255)                                                                                                                aaaGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACt                       >  3:1858/1‑149 (MQ=255)                                                                                                                    ttATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTcaca                   >  4:284737/1‑149 (MQ=255)                                                                                                                        atataACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAata               <  2:250901/149‑1 (MQ=255)                                                                                                                         tataACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAatat              >  1:589738/1‑149 (MQ=255)                                                                                                                          ataACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATAtt             >  3:679520/1‑149 (MQ=255)                                                                                                                          ataACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATAtt             >  3:681897/1‑149 (MQ=255)                                                                                                                           taACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTc            >  2:646934/1‑149 (MQ=255)                                                                                                                           taACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTc            <  1:116287/149‑1 (MQ=255)                                                                                                                           taACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTc            <  1:907007/149‑1 (MQ=255)                                                                                                                           taACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTc            >  1:2197/1‑149 (MQ=255)                                                                                                                           taACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTc            >  4:369213/1‑149 (MQ=255)                                                                                                                           taACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTc            >  2:907007/1‑149 (MQ=255)                                                                                                                             aCAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTg          >  2:667164/1‑149 (MQ=255)                                                                                                                             aCAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTg          >  1:982765/1‑149 (MQ=255)                                                                                                                              cAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTgg         >  1:434115/1‑149 (MQ=255)                                                                                                                               aaaTCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGc        >  1:56297/1‑149 (MQ=255)                                                                                                                                aaTCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAACCTCACAAATATTCTGGCa       >  1:1103306/1‑149 (MQ=255)                                                                                                                                  tCCCAATAACTTAATTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTca                     <  2:69514/133‑1 (MQ=255)                                                                                                                                  tCCCAATAACTTAATTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTca                     >  1:69514/1‑133 (MQ=255)                                                                                                                                     cAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCTGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGt  >  3:766160/1‑149 (MQ=255)                                                                                                                                     cAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGt  >  3:600849/1‑149 (MQ=255)                                                                                                                                     cAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGt  >  1:829111/1‑149 (MQ=255)                                                                                                                                     cAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTACCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGc                                                <  2:903870/103‑1 (MQ=255)                                                                                                                                     cAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTACCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGc                                                >  1:903870/1‑103 (MQ=255)                                                                                                                                      aataatTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGa                                                            <  3:320195/90‑1 (MQ=255)                                                                                                                                      aataatTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGa                                                            >  4:320195/1‑90 (MQ=255)                                                                                                                                           |                                                                                                                                               ATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGT  >  NC_000913/3906688‑3906968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑