Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,060,261 | G→T | 11.6% | intergenic (+31/‑186) | typA → / → yihL | GTP‑binding protein/putative DNA‑binding transcriptional regulator |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,060,261 | 0 | G | T | 11.6% | 374.4 / 33.5 | 112 | intergenic (+31/‑186) | typA/yihL | GTP‑binding protein/putative DNA‑binding transcriptional regulator |
Reads supporting (aligned to +/- strand): ref base G (51/48); new base T (6/7); total (57/55) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.74e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.48e-01 |
Only 100 of 112 total aligned reads displayed.
TTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCTTTTTTGGCGTAAGCG > NC_000913/4060117‑4060407 | ttCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAt > 2:281605/1‑149 (MQ=255) ttCATCGATCATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAt < 4:750109/149‑1 (MQ=255) tCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTGCGTTTTTTAGTCATAAAAAACCTGCCAGCGATg < 1:881156/149‑1 (MQ=255) cATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATgg > 4:643892/1‑149 (MQ=255) aTCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATgg < 3:623357/148‑1 (MQ=255) aTCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATgg > 4:623357/1‑148 (MQ=255) tCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCa < 3:127902/149‑1 (MQ=255) cGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAg < 1:933196/149‑1 (MQ=255) cGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAg < 1:339959/149‑1 (MQ=255) gatgatGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAgg < 3:524889/149‑1 (MQ=255) gatgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑ttt < 4:18566/149‑1 (MQ=255) gaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑tttttt > 1:129415/1‑149 (MQ=255) gTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAg < 1:342081/133‑1 (MQ=255) gTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAg > 2:342081/1‑133 (MQ=255) aGAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGc > 1:1130156/1‑143 (MQ=255) aGAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGc < 2:1130156/143‑1 (MQ=255) gAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑ttttttttt < 4:189904/140‑1 (MQ=255) gAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑ttttttttt > 3:189904/1‑140 (MQ=255) gAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTg > 1:483660/1‑149 (MQ=255) aGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGa < 1:837961/149‑1 (MQ=255) gTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAg > 4:819884/1‑127 (MQ=255) gTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAg < 3:819884/127‑1 (MQ=255) gTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGc > 1:1048627/1‑139 (MQ=255) gTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGc < 2:1048627/139‑1 (MQ=255) tCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGcac < 4:567724/149‑1 (MQ=255) tctATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATg > 2:12621/1‑149 (MQ=255) ctATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGc < 4:305095/149‑1 (MQ=255) tATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCg > 1:1103255/1‑149 (MQ=255) aTCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGa < 3:629593/133‑1 (MQ=255) aTCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGa > 4:629593/1‑133 (MQ=255) aTCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGa < 2:1103255/149‑1 (MQ=255) cGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTa > 4:772465/1‑149 (MQ=255) gTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAt > 4:792644/1‑149 (MQ=255) tATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGt > 3:953142/1‑126 (MQ=255) aTTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtct > 4:39083/1‑149 (MQ=255) aTTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCAGGCGAGGGCAGT‑TTTTTTTTTCCCGGTCTTGAGGCCCCACAAATGGGAGTAtct > 3:146615/1‑149 (MQ=255) ttCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGc < 2:125572/140‑1 (MQ=255) ttCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGc > 1:125572/1‑140 (MQ=255) tCGTAAACGTTAACCTAAC‑GAAAACGGTCCTCGCCGGCGCAACCCGGCAACGAAAGGAGATTTATTTCGGTTTTTTATCAAAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTcc < 4:146615/149‑1 (MQ=255) aaaCG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtat < 2:483660/149‑1 (MQ=255) g‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTc < 1:857009/113‑1 (MQ=255) g‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTc > 2:857009/1‑113 (MQ=255) aCCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCt < 3:772465/149‑1 (MQ=255) cTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgt > 4:363710/1‑149 (MQ=255) cTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgt > 2:484927/1‑149 (MQ=255) cGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGtt < 4:481261/149‑1 (MQ=255) ggAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTa > 3:66378/1‑149 (MQ=255) ggAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTa > 3:863245/1‑149 (MQ=255) gAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAc > 1:1081739/1‑149 (MQ=255) gAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAc > 4:771917/1‑149 (MQ=255) aaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGt > 2:728/1‑149 (MQ=255) aaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGt < 4:863245/149‑1 (MQ=255) aaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGt < 1:12621/149‑1 (MQ=255) aaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGG‑tt < 4:408953/85‑1 (MQ=255) aaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGG‑tt > 3:408953/1‑85 (MQ=255) aaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTTGCCTGTGGTGAGCGCACACCAATGCGAGTATTTCCCTTATAAGTCTTTGGTTACGt > 2:828771/1‑149 (MQ=255) aaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAc > 1:725378/1‑146 (MQ=255) aaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCACACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTc > 4:670559/1‑149 (MQ=255) gATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAt > 2:452718/1‑149 (MQ=255) cgccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑ttttttt > 1:824484/1‑81 (MQ=255) cgccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑ttttttt < 2:824484/81‑1 (MQ=255) cgccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTa > 2:10353/1‑136 (MQ=255) cgccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTa < 1:10353/136‑1 (MQ=255) cgccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag < 4:513785/149‑1 (MQ=255) gccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagag < 3:317192/146‑1 (MQ=255) gccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagag > 4:317192/1‑146 (MQ=255) gcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGt < 3:423222/149‑1 (MQ=255) cAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTca < 3:102700/149‑1 (MQ=255) ccGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACaa < 1:452718/149‑1 (MQ=255) cgcgCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAAt < 2:967839/149‑1 (MQ=255) gcACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTAt < 3:771917/149‑1 (MQ=255) aCCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCt > 2:1070612/1‑149 (MQ=255) aaGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAgc < 3:483145/74‑1 (MQ=255) aaGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAgc > 4:483145/1‑74 (MQ=255) aaGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCaaa < 3:643892/149‑1 (MQ=255) cGATTAATTTCGTTTTTTAGTCATAAACAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCa < 3:792644/149‑1 (MQ=255) ttCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGt > 1:471029/1‑149 (MQ=255) aGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGcttt < 2:1081739/149‑1 (MQ=255) cATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCt > 1:236106/1‑119 (MQ=255) cATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGctttctt < 2:129415/149‑1 (MQ=255) aTAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGctttcttt > 2:933141/1‑149 (MQ=255) aaaaaCCTGCCATCGCTGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCtttttt > 3:716317/1‑149 (MQ=255) aaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCTTTTTTg < 3:39083/149‑1 (MQ=255) aaaCCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGc < 1:599942/115‑1 (MQ=255) aaaCCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGc > 2:599942/1‑115 (MQ=255) aaaCCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGctttc > 3:1053075/1‑141 (MQ=255) aaaCCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGctttc < 4:1053075/141‑1 (MQ=255) aaaCCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCTTTTTTgg > 2:575636/1‑149 (MQ=255) aaaCCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCTTTTTTgg > 1:664706/1‑149 (MQ=255) aCCTGCCATCGCTGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgtg < 3:952960/77‑1 (MQ=38) aCCTGCCATCGCTGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgtg > 4:952960/1‑77 (MQ=38) aCCTGCCATCGCTGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGctttcttt > 4:54/1‑142 (MQ=255) aCCTGCCATCGCTGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGctttcttt < 3:54/142‑1 (MQ=255) aCCTGCCATCGCTGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGcttt > 2:953020/1‑138 (MQ=255) aCCTGCCATCGCTGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGcttt < 1:953020/138‑1 (MQ=255) ccTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtc > 2:920507/1‑59 (MQ=255) ccTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtc < 1:920507/59‑1 (MQ=255) tccAGCGATGGCAGGTTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCTTTTTTGgcgtaa < 1:828771/148‑1 (MQ=255) gCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCTTTTTTGgcgtaag > 3:90765/1‑149 (MQ=255) aaGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCTTTTTTGgcgtaagcg > 2:723499/2‑149 (MQ=255) | TTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACG‑TCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCTTTTTTGGCGTAAGCG > NC_000913/4060117‑4060407 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |