Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,127,252 A→G 8.0% intergenic (+27/+34) rpmE → / ← yiiX 50S ribosomal subunit protein L31/putative lipid binding hydrolase, DUF830 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,127,2520AG 8.0% 155.2 / 4.3 50intergenic (+27/+34)rpmE/yiiX50S ribosomal subunit protein L31/putative lipid binding hydrolase, DUF830 family protein
Reads supporting (aligned to +/- strand):  ref base A (26/20);  new base G (2/2);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

CCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTCT  >  NC_000913/4127111‑4127400
                                                                                                                                             |                                                                                                                                                    
ccTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCt                                                                                                                                               >  4:1114108/1‑149 (MQ=255)
    gACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCttttt                                                                                                                                           >  3:33527/1‑149 (MQ=255)
                       aCCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGCAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGTATTTTGGTc                                                                                                                        >  1:856866/1‑149 (MQ=255)
                         ccGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGt                                                                                                                      >  1:193900/1‑149 (MQ=255)
                          cGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAAtt                                                                                                                              <  4:801199/140‑1 (MQ=255)
                          cGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAAtt                                                                                                                              >  3:801199/1‑140 (MQ=255)
                                    tGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGTGCGGCGCttttttc                                                                                                                                         >  3:1026658/1‑118 (MQ=255)
                                    tGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGTGCGGCGCttttttc                                                                                                                                         <  4:1026658/119‑2 (MQ=255)
                                    tGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGaa                                                                                                           >  4:794564/1‑149 (MQ=255)
                                    tGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGaa                                                                                                           >  4:353144/1‑149 (MQ=255)
                                      gCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTg                                                                                                                           <  3:1092033/131‑1 (MQ=255)
                                      gCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTg                                                                                                                           >  4:1092033/1‑131 (MQ=255)
                                         aaCAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAGGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCttttttt                                                                                                                                         >  1:166748/1‑114 (MQ=255)
                                         aaCAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAGGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCttttttt                                                                                                                                         <  2:166748/114‑1 (MQ=255)
                                          aCAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGaaaa                                                                                                         <  2:144060/145‑1 (MQ=255)
                                          aCAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGaaaa                                                                                                         >  1:144060/1‑145 (MQ=255)
                                                         tACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTa                                                                                                                     >  1:712482/1‑118 (MQ=255)
                                                         tACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTa                                                                                                                     <  2:712482/118‑1 (MQ=255)
                                                                   cGTGTTGACCGCTTCGACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGa                                                                            >  4:687337/1‑149 (MQ=255)
                                                                             gCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGgcgc                                                                  >  4:968811/1‑149 (MQ=255)
                                                                                   acaaGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGaa                                                            >  4:508256/1‑149 (MQ=255)
                                                                                     aaGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGaaaa                                                          >  3:204301/1‑149 (MQ=255)
                                                                                             cAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTtgt                                                  >  4:1085601/1‑149 (MQ=255)
                                                                                               aCATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCTGCACGGCGCTTTATTTGTACCTGAATTTTGATGGGATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGCTGAGTTGTGTCGCATCGAAAACAGCTTGTgg                                                >  3:708782/1‑149 (MQ=255)
                                                                                                cATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGc                                               <  3:1114108/149‑1 (MQ=255)
                                                                                                cATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGc                                               <  2:171559/149‑1 (MQ=255)
                                                                                                cATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGc                                               <  2:193900/149‑1 (MQ=255)
                                                                                                 aTCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTg                                                                                                                           <  3:91621/72‑1 (MQ=255)
                                                                                                 aTCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTg                                                                                                                           >  4:91621/1‑72 (MQ=255)
                                                                                                  tCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCga                                             >  1:592773/1‑149 (MQ=255)
                                                                                                        gcagcaAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGaccac                                       <  3:794564/149‑1 (MQ=255)
                                                                                                            caAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGtt                                   <  3:687337/149‑1 (MQ=255)
                                                                                                             aaaTAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGac                                          >  3:111651/1‑141 (MQ=255)
                                                                                                             aaaTAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGac                                          <  4:111651/141‑1 (MQ=255)
                                                                                                             aaaTAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGttt                                  >  4:524020/1‑149 (MQ=255)
                                                                                                              aaTAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTc                                 <  3:524020/149‑1 (MQ=255)
                                                                                                               aTAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTcc                                <  3:353144/149‑1 (MQ=255)
                                                                                                               aTAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTcc                                >  2:366542/1‑149 (MQ=255)
                                                                                                                tAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCga                                             <  4:673246/135‑1 (MQ=255)
                                                                                                                tAAGACCGGATATCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCga                                             >  3:673246/1‑135 (MQ=255)
                                                                                                                     ccGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGc                          <  1:366542/149‑1 (MQ=255)
                                                                                                                      cGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTg                                                                                                                           <  1:207056/51‑1 (MQ=255)
                                                                                                                      cGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTg                                                                                                                           >  2:207056/1‑51 (MQ=255)
                                                                                                                           ttCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAata                    <  2:856866/149‑1 (MQ=255)
                                                                                                                               gAAAAAAGCGACGCACGGCGGTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAATCAGCGGTCATGCTTTGGCTACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCTCCGTTTCCTTAACCGGAATATTCt                <  4:33527/149‑1 (MQ=255)
                                                                                                                                  aaaaGCGCCGTGCGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTtgtg                                                 >  4:1116884/1‑113 (MQ=255)
                                                                                                                                  aaaaGCGCCGTGCGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTtgtg                                                 <  3:1116884/113‑1 (MQ=255)
                                                                                                                                  aaaaGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAACCAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTc                                 <  2:48250/129‑1 (MQ=255)
                                                                                                                                  aaaaGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCGTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTc                                 >  1:48250/1‑129 (MQ=255)
                                                                                                                                           gcACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGtt    >  1:1059975/1‑149 (MQ=255)
                                                                                                                                             aCGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCATTGGCGACCGGGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTCt  >  3:117161/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
CCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTCT  >  NC_000913/4127111‑4127400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑