BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,241,715	G→T	6.5%	C180* (TGC→TGA) ‡	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,715	0	G	T	6.5%	438.6 / 15.5	123	C180* (TGC→TGA) ‡	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand): ref base G (56/59); new base T (3/5); total (59/64)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 126 total aligned reads displayed.

GCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAA  >  NC_000913/4241567‑4241863

gcgACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACg                                                                                                                                                      <  4:387204/149‑1 (MQ=255)

     aTCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTa                                                                                                                                                 <  3:27387/149‑1 (MQ=255)

      tCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTaa                                                                                                                                                >  1:387770/1‑149 (MQ=255)

       cagccagcGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTaaa                                                                                                                                               <  1:1146226/149‑1 (MQ=255)

        agccagcGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAAc                                                                                                                                              >  2:1058540/1‑149 (MQ=255)

           cagcGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACTGCGTAAACTat                                                                                                                                           <  1:857239/149‑2 (MQ=255)

           cagcGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTaa                                                                                                                                           >  1:78487/1‑149 (MQ=255)

            agcGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTaaa                                                                                                                                          >  2:101876/1‑149 (MQ=255)

             gcGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACTGCGTAAACTattt                                                                                                                                         <  1:763865/149‑4 (MQ=255)

             gcGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTaaaa                                                                                                                                         <  2:387770/149‑1 (MQ=255)

                 ggACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTg                                                                                                                                     <  4:569820/149‑1 (MQ=255)

                 ggACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTg                                                                                                                                     <  1:101876/149‑1 (MQ=255)

                  gACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGc                                                                                                                                    >  1:822297/1‑149 (MQ=255)

                  gACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGc                                                                                                                                    <  1:1013283/149‑1 (MQ=255)

                     tttCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTaaaa                                                                                                                                         <  4:417585/141‑1 (MQ=255)

                     tttCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCg                                                                                                                                 >  3:944674/1‑149 (MQ=255)

                     tttCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCg                                                                                                                                 >  4:950051/1‑149 (MQ=255)

                     tttCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCg                                                                                                                                 >  1:391946/1‑149 (MQ=255)

                         tGGCACGGTATACAGCAGCATTAAGAACGGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGaaaa                                                                                                                             <  2:773138/149‑1 (MQ=255)

                            cACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAaataa                                                                                                                          <  3:364009/149‑1 (MQ=255)

                             aCGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAaataat                                                                                                                         >  2:954940/1‑149 (MQ=255)

                              cGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATc                                                                                                                        <  3:154469/149‑1 (MQ=255)

                                 tataCAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCa                                                                                                                     >  3:895052/1‑149 (MQ=255)

                                      agcagcATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTg                                                                                                                >  4:225275/1‑149 (MQ=255)

                                        cagcaTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGt                                                                                                              <  1:550316/149‑1 (MQ=255)

                                        cagcaTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGt                                                                                                              <  1:351878/149‑1 (MQ=255)

                                          gcaTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGc                                                                                                                                    <  3:706571/125‑1 (MQ=255)

                                          gcaTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGc                                                                                                                                    >  4:706571/1‑125 (MQ=255)

                                            aTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGc                                                                                                                      >  1:545488/1‑137 (MQ=255)

                                            aTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGc                                                                                                                      <  2:545488/137‑1 (MQ=255)

                                            aTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTaaa                                                                                                          >  2:456638/1‑149 (MQ=255)

                                            aTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTaaa                                                                                                          <  3:615634/149‑1 (MQ=255)

                                             ttAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTaaaa                                                                                                         <  4:8910/149‑1 (MQ=255)

                                               aaGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAaga                                                                                                       <  2:299004/149‑1 (MQ=255)

                                               aaGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAaga                                                                                                       <  2:7131/149‑1 (MQ=255)

                                                aGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAagag                                                                                                      <  2:391946/149‑1 (MQ=255)

                                                aGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAagag                                                                                                      <  2:1088772/149‑1 (MQ=255)

                                                 gAACAGCAGTGCAGGGATACATTCCGTGGTAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACTGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAACTTGCCCGAAAATAATCGCAGACTGGTTAAAAgaga                                                                                                     <  4:709510/149‑1 (MQ=255)

                                                      gcagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGt                                                                                                >  4:700833/1‑149 (MQ=255)

                                                           gCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTccc                                                                                           <  2:577851/149‑1 (MQ=255)

                                                            cAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCcg                                                                                          <  2:822297/149‑1 (MQ=255)

                                                             aGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCcgc                                                                                         >  4:670539/1‑149 (MQ=255)

                                                             aGGGATACATGCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCcgc                                                                                         >  3:776229/1‑149 (MQ=255)

                                                               ggATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGa                                                                                       >  3:451392/1‑149 (MQ=255)

                                                               ggATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGa                                                                                       >  3:826462/1‑149 (MQ=255)

                                                               ggATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGa                                                                                       >  1:237657/1‑149 (MQ=255)

                                                               ggATACATTCCGAGCCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCCCCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAAACGCAAACTGGTTAAAAGAGACCAGTTTCCCCCGa                                                                                       >  2:965659/1‑149 (MQ=255)

                                                                gATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGaa                                                                                      <  4:1104869/149‑1 (MQ=255)

                                                                 aTACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTc                                                                                             <  3:816755/141‑1 (MQ=255)

                                                                 aTACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTc                                                                                             >  4:816755/1‑141 (MQ=255)

                                                                     aTTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGa                                                                                 >  4:1105915/1‑149 (MQ=255)

                                                                      ttCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAagag                                                                                                      >  3:617246/1‑127 (MQ=255)

                                                                      ttCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAagag                                                                                                      <  4:617246/127‑1 (MQ=255)

                                                                       tCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGc                                                                               <  3:182671/149‑1 (MQ=255)

                                                                        ccGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCAGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCt                                                                              >  4:45010/1‑149 (MQ=255)

                                                                          gAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTgg                                                                            >  4:367479/1‑149 (MQ=255)

                                                                               aaaCATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCt                                                                              <  4:1031469/142‑1 (MQ=255)

                                                                               aaaCATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCa                                                                       >  1:163063/1‑149 (MQ=255)

                                                                               aaaCATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCa                                                                       >  2:112201/1‑149 (MQ=255)

                                                                                          gCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACTGCGTAAACTattt                                                                                                                                         <  1:1075467/72‑4 (MQ=38)

                                                                                          gCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACTGCGTAAACTattt                                                                                                                                         >  2:1075467/1‑69 (MQ=38)

                                                                                          gCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAata                                                            <  3:1105915/149‑1 (MQ=255)

                                                                                             aGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATAc                                                         <  2:163063/149‑1 (MQ=255)

                                                                                             aGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATAc                                                         <  3:670539/149‑1 (MQ=255)

                                                                                             aGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATAc                                                         <  1:456638/149‑1 (MQ=255)

                                                                                              gCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTaaaa                                                                                                         <  3:259131/100‑1 (MQ=255)

                                                                                              gCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTaaaa                                                                                                         >  4:259131/1‑100 (MQ=255)

                                                                                               ccGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACAt                                                       <  3:623448/149‑1 (MQ=255)

                                                                                                cGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACAtt                                                      <  2:172907/149‑1 (MQ=255)

                                                                                                 gTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTg                                                     <  3:700833/149‑1 (MQ=255)

                                                                                                  tCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATAc                                                         <  1:53986/144‑1 (MQ=255)

                                                                                                   cAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGc                                                   <  3:367479/149‑1 (MQ=255)

                                                                                                     gTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCga                                                 >  3:919669/1‑149 (MQ=255)

                                                                                                          cagccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCa                                            <  4:776229/149‑1 (MQ=255)

                                                                                                           agccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTc                                                                   >  3:213196/1‑125 (MQ=255)

                                                                                                           agccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTc                                                                   <  4:213196/125‑1 (MQ=255)

                                                                                                           agccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCAt                                           >  3:833965/1‑149 (MQ=255)

                                                                                                            gccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGa                                                                           >  1:1001189/1‑116 (MQ=255)

                                                                                                            gccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGa                                                                           <  2:1001189/116‑1 (MQ=255)

                                                                                                            gccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCAtt                                          <  3:950051/149‑1 (MQ=255)

                                                                                                              cagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGa                                        <  4:944674/149‑1 (MQ=255)

                                                                                                                       tCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAAcc                               >  3:454453/1‑149 (MQ=255)

                                                                                                                          ccGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAAc                            <  3:1111492/149‑1 (MQ=255)

                                                                                                                           cGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACg                           >  2:489892/1‑149 (MQ=255)

                                                                                                                                 gggCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATa                     >  4:1138363/1‑149 (MQ=255)

                                                                                                                                  ggCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATAc                    >  3:1023867/1‑149 (MQ=255)

                                                                                                                                  ggCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATAc                    <  4:826462/149‑1 (MQ=255)

                                                                                                                                     aaTAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATa                     >  4:606485/1‑145 (MQ=255)

                                                                                                                                     aaTAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATa                     <  3:606485/145‑1 (MQ=255)

                                                                                                                                     aaTAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGc                 >  1:1061726/1‑149 (MQ=255)

                                                                                                                                       tAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTcc                                                                  >  4:556170/1‑98 (MQ=255)

                                                                                                                                       tAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTcc                                                                  <  3:556170/98‑1 (MQ=255)

                                                                                                                                        aaaaTAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCaa              <  3:45010/149‑1 (MQ=255)

                                                                                                                                        aaaaTAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCaa              <  3:1048254/149‑1 (MQ=255)

                                                                                                                                           aTAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCaataa           >  3:674443/1‑149 (MQ=255)

                                                                                                                                                ttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGa      <  3:1138363/149‑1 (MQ=255)

                                                                                                                                                ttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGa      <  1:1066097/149‑1 (MQ=255)

                                                                                                                                                 tACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGat     <  2:1061726/149‑1 (MQ=255)

                                                                                                                                                    gCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaa  <  2:237657/149‑1 (MQ=255)

                                                                                                                                                    gCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaa  <  4:919669/149‑1 (MQ=255)

GCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAA  >  NC_000913/4241567‑4241863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`