Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,356,446 A→T 14.0% intergenic (‑35/+24) dtpC ← / ← cadA dipeptide and tripeptide permease/lysine decarboxylase, acid‑inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,356,4460AT14.0% 137.7 / 16.2 50intergenic (‑35/+24)dtpC/cadAdipeptide and tripeptide permease/lysine decarboxylase, acid‑inducible
Reads supporting (aligned to +/- strand):  ref base A (21/22);  new base T (4/3);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.00e-01

CTGATGGGTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCA  >  NC_000913/4356298‑4356591
                                                                                                                                                    |                                                                                                                                                 
cTGATGGGTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCaa                                                                                                                                                   <  1:1075499/149‑1 (MQ=255)
cTGATGGGTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCaa                                                                                                                                                   <  3:279444/149‑1 (MQ=255)
       gTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACtt                                                                                                                                            >  4:1089953/1‑149 (MQ=255)
        tGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCTTGCCACTTc                                                                                                                                           <  3:939118/149‑1 (MQ=255)
              agagAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACtt                                                                                                                                            <  1:102239/142‑1 (MQ=255)
              agagAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACtt                                                                                                                                            >  2:102239/1‑142 (MQ=255)
              agagAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGt                                                                                                                                     >  3:86890/1‑149 (MQ=255)
                           ggCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAattat                                                                                                                        >  4:561535/1‑149 (MQ=255)
                              aCGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTAtttt                                                                                                                     <  2:692215/149‑1 (MQ=255)
                              aCGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTAtttt                                                                                                                     <  1:632121/149‑1 (MQ=255)
                                           aaaCTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTcttctt                                                                                                        <  4:891684/149‑1 (MQ=255)
                                            aaCTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGt                                                                                                                                     >  4:882385/1‑119 (MQ=255)
                                            aaCTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGt                                                                                                                                     <  3:882385/119‑1 (MQ=255)
                                            aaCTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCttt                                                                                                       >  3:600125/1‑149 (MQ=255)
                                            aaCTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGAAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCttt                                                                                                       >  4:1068792/1‑149 (MQ=255)
                                             aCTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTc                                                                                                      <  4:11182/149‑1 (MQ=255)
                                               tGAAGTACTCCCAGATTTGTATCGCCACGATTTAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCTTGCCACtt                                                                                                                                            <  2:821182/109‑1 (MQ=255)
                                               tGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCTTGCCACtt                                                                                                                                            >  1:821182/1‑109 (MQ=255)
                                                       tCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTaa                                                                                            >  3:102355/1‑149 (MQ=255)
                                                        cccAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTatc                                                                                           <  1:910574/149‑3 (MQ=255)
                                                         ccAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACg                                                                                          >  3:1043359/1‑149 (MQ=255)
                                                                   aTCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCAc                                                                                                                                              <  3:347294/87‑1 (MQ=255)
                                                                   aTCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCAc                                                                                                                                              >  4:347294/1‑87 (MQ=255)
                                                                    tCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGcc                                                                               <  4:1043359/149‑1 (MQ=255)
                                                                                aGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTaa                                                                                                                            <  1:497981/92‑1 (MQ=255)
                                                                                aGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTaa                                                                                                                            >  2:497981/1‑92 (MQ=255)
                                                                                 gTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACgg                                                                  >  1:97373/1‑149 (MQ=255)
                                                                                     atCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTtcttct                                                                                                         <  3:28552/106‑1 (MQ=255)
                                                                                     atCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTtcttct                                                                                                         >  4:28552/1‑106 (MQ=255)
                                                                                          gcgcgGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCt                                                                                               >  4:930266/1‑111 (MQ=255)
                                                                                          gcgcgGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCt                                                                                               <  3:930266/111‑1 (MQ=255)
                                                                                          gcgcgGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCCCCg                                                         <  4:600125/149‑1 (MQ=255)
                                                                                                     aGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAAt                                                                                                   <  4:835521/96‑1 (MQ=255)
                                                                                                     aGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAAt                                                                                                   >  3:835521/1‑96 (MQ=255)
                                                                                                     aGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGt                                              <  4:110246/149‑1 (MQ=255)
                                                                                                        gtgtTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTc                                           >  1:844144/1‑149 (MQ=255)
                                                                                                               cATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGccc                                    <  2:97373/149‑1 (MQ=255)
                                                                                                               cATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGccc                                    <  3:1089953/149‑1 (MQ=255)
                                                                                                                  gtgtTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGa                                 <  3:1068792/149‑1 (MQ=255)
                                                                                                                   tgtTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGa                                 <  4:391208/148‑1 (MQ=255)
                                                                                                                   ggtTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGa                                 >  3:391208/2‑148 (MQ=255)
                                                                                                                         ccTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAg                          <  4:86890/149‑1 (MQ=255)
                                                                                                                           ttATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGtt                                             <  3:402567/128‑1 (MQ=255)
                                                                                                                           ttATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGtt                                             >  4:402567/1‑128 (MQ=255)
                                                                                                                           ttATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCACTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAgcg                        >  1:236600/1‑149 (MQ=255)
                                                                                                                                  cAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGAttt                 <  4:102355/149‑1 (MQ=255)
                                                                                                                                       aaGGGAAGTGGCTTGCCACTTCCCTTGTACGAGCTGATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGAttt                 <  3:439375/144‑1 (MQ=255)
                                                                                                                                       aaGGGAAGTGGCTTGCCACTTCCCTTGTACGAGCTGATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGAttt                 >  4:439375/1‑144 (MQ=255)
                                                                                                                                         gggAAGTGGCTTGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAg          >  2:823898/1‑149 (MQ=255)
                                                                                                                                           gAAGTGGCTTGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCa        >  4:949018/1‑149 (MQ=255)
                                                                                                                                           gAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCATTATTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCa        >  2:336854/1‑149 (MQ=255)
                                                                                                                                                 gCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGCTATAGCGGCCATCAGCCTGAGGGTATGCACTGTGAATATCGGTTTCAACGTCCGGATCGTGAGCGCCGATGTCACACAGCACCTGCa  >  3:1092077/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CTGATGGGTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCA  >  NC_000913/4356298‑4356591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑