Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,376,280 G→A 5.6% intergenic (+15/‑37) efp → / → ecnA polyproline‑specific translation elongation factor EF‑P/entericidin A membrane lipoprotein, antidote entericidin B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,376,2800GA 5.6% 478.7 / 10.3 143intergenic (+15/‑37)efp/ecnApolyproline‑specific translation elongation factor EF‑P/entericidin A membrane lipoprotein, antidote entericidin B
Reads supporting (aligned to +/- strand):  ref base G (63/71);  new base A (2/6);  total (65/78)
Fisher's exact test for biased strand distribution p-value = 2.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 146 total aligned reads displayed.

ATACCGCAGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTC  >  NC_000913/4376132‑4376428
                                                                                                                                                    |                                                                                                                                                    
aTACCGCAGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCg                                                                                                                                                      >  2:631437/1‑149 (MQ=255)
  aCCGCAGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGc                                                                                                                                                    >  4:1052009/1‑149 (MQ=255)
   ccGCAGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGCATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGcc                                                                                                                                                   >  3:964001/1‑149 (MQ=255)
   ccGCAGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGcc                                                                                                                                                   >  3:14119/1‑149 (MQ=255)
   ccGCAGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGcc                                                                                                                                                   <  2:334349/149‑1 (MQ=255)
   ccGCAGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGcc                                                                                                                                                   <  1:184476/149‑1 (MQ=255)
    cGCAGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCt                                                                                                                                                  >  3:924593/1‑149 (MQ=255)
     gCAGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTg                                                                                                                                                 >  1:796915/1‑149 (MQ=255)
            cTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTg                                                                                                                                          <  1:1104463/149‑1 (MQ=255)
              ggtggCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCa                                                                                                                                        <  2:588178/149‑1 (MQ=255)
              ggtggCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCa                                                                                                                                        <  3:54844/149‑1 (MQ=255)
               gtggCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCAc                                                                                                                                       >  1:995744/1‑149 (MQ=255)
                   cAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATc                                                                                                                                   <  3:314534/149‑1 (MQ=255)
                    aaaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                                                                  >  1:101487/1‑149 (MQ=255)
                    aaaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                                                                  >  1:30131/1‑149 (MQ=255)
                    aaaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                                                                  >  1:570998/1‑149 (MQ=255)
                    aaaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                                                                  >  1:913312/1‑149 (MQ=255)
                     aaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCAc                                                                                                                                 >  2:857186/1‑149 (MQ=255)
                     aaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCAc                                                                                                                                 <  2:887338/149‑1 (MQ=255)
                        cGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTa                                                                                                                              <  3:1052009/149‑1 (MQ=255)
                        cGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTa                                                                                                                              <  1:1090908/149‑1 (MQ=255)
                          gCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTAtt                                                                                                                            <  1:191851/149‑1 (MQ=255)
                            tACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCa                                                                                                                          >  3:700795/1‑149 (MQ=255)
                             aCCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAg                                                                                                                         <  4:853667/149‑1 (MQ=255)
                                cTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTAtt                                                                                                                            <  3:502687/143‑1 (MQ=255)
                                cTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTc                                                                                                                      <  2:796915/149‑1 (MQ=255)
                                  gTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCag                                                                                                                    >  2:249731/1‑149 (MQ=255)
                                   tCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCaga                                                                                                                   <  2:456261/149‑1 (MQ=255)
                                       cTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgatg                                                                                                               <  1:831617/149‑1 (MQ=255)
                                        tGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgatga                                                                                                              <  2:569638/149‑1 (MQ=255)
                                        tGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgatga                                                                                                              <  1:1126308/149‑1 (MQ=255)
                                         ggCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgatgat                                                                                                             >  2:1018907/1‑149 (MQ=255)
                                          gcgcTGTGGTTAAAGTGCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGGTATTCGATCTCGTGAATAAGTCTCTCGCGTGAAGTAATACGGTTATGGTGCGGCCTGCAGGCTGCACCATCACTGATTTACGTCTgagacatcg                                                                                                            >  2:149675/1‑144 (MQ=255)
                                            gcTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAgg                                                                                                                                             >  2:633102/1‑114 (MQ=255)
                                            gcTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAgg                                                                                                                                             <  1:633102/114‑1 (MQ=255)
                                              tgtgGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAAc                                                                                                        <  1:857186/149‑1 (MQ=255)
                                                tgGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGc                                                                                                      <  2:824731/149‑1 (MQ=255)
                                                 ggTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGcc                                                                                                     <  4:76202/149‑1 (MQ=255)
                                                   ttgaaGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCa                                                                                                                                               <  1:337940/102‑1 (MQ=255)
                                                   ttAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCa                                                                                                                                               >  2:337940/1‑105 (MQ=255)
                                                   ttAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCtt                                                                                                   >  3:591686/1‑149 (MQ=255)
                                                           ccGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATAGTTCt                                                                                           <  4:1051374/149‑1 (MQ=255)
                                                             gCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTg                                                                                         >  2:400703/1‑149 (MQ=255)
                                                              cTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGt                                                                                        >  4:805543/1‑149 (MQ=255)
                                                               tGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGtt                                                                                       <  2:240754/149‑1 (MQ=255)
                                                                gTTTGTACACATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGtct                                                                                      <  2:913312/149‑3 (MQ=255)
                                                                gTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTATGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGttt                                                                                      <  4:964001/149‑1 (MQ=255)
                                                                    gTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATc                                                                                                                                   <  3:771267/100‑1 (MQ=255)
                                                                     tACCAATCCGCGGAGTCATCAAAGTGGATTCCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgctt                                                                                 <  4:591686/149‑1 (MQ=255)
                                                                     tACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgctt                                                                                 >  4:896545/1‑149 (MQ=255)
                                                                      aCAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttg                                                                                >  1:1133129/1‑149 (MQ=255)
                                                                      aCAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttg                                                                                >  3:420717/1‑149 (MQ=255)
                                                                      aCAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttg                                                                                >  1:350915/1‑149 (MQ=255)
                                                                        aaaTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGcc                                                                              <  1:400703/149‑1 (MQ=255)
                                                                              gCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAg                                                                                                                                              >  1:700791/1‑79 (MQ=255)
                                                                              gCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAg                                                                                                                                              <  2:700791/79‑1 (MQ=255)
                                                                               cGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACtt                                                                                                                               >  2:837908/1‑93 (MQ=255)
                                                                               cGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACtt                                                                                                                               <  1:837908/93‑1 (MQ=255)
                                                                                 aaGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgctg                                                                     <  4:924593/149‑1 (MQ=255)
                                                                                 aaGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGGAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgctg                                                                     <  2:1067840/149‑1 (MQ=255)
                                                                                  aGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgctgc                                                                    >  3:399798/1‑149 (MQ=255)
                                                                                   gTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGtttt                                                                                     >  1:1043603/1‑131 (MQ=255)
                                                                                   gTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGtttt                                                                                     <  2:1043603/131‑1 (MQ=255)
                                                                                    tcatcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTc                                                                  >  4:123002/1‑149 (MQ=255)
                                                                                       tcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgct                                                                                  >  3:649518/1‑130 (MQ=255)
                                                                                       tcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgct                                                                                  <  4:649518/130‑1 (MQ=255)
                                                                                          aaGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGtt                                                                                       <  3:891170/122‑1 (MQ=255)
                                                                                          aaGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGtt                                                                                       >  4:891170/1‑122 (MQ=255)
                                                                                           aGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCt                                                           <  1:1018907/149‑1 (MQ=255)
                                                                                                  aCCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgc                                                                                   <  2:538195/118‑1 (MQ=255)
                                                                                                  aCCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAacac                                                    <  4:700795/149‑1 (MQ=255)
                                                                                                   cccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCt                                                           >  4:455747/1‑141 (MQ=255)
                                                                                                   cccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAacac                                                    <  3:292970/148‑1 (MQ=255)
                                                                                                   cccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAacac                                                    >  4:292970/1‑148 (MQ=255)
                                                                                                   cccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACAcc                                                   >  3:881062/1‑149 (MQ=255)
                                                                                                    ccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATc                                                                                                                                   >  4:675307/1‑68 (MQ=255)
                                                                                                    ccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCTGCACCATc                                                                                                                                   <  3:675307/68‑1 (MQ=255)
                                                                                                    ccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCAc                                                                                                                                       >  3:610911/1‑64 (MQ=255)
                                                                                                    ccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCAc                                                                                                                                       <  4:610911/64‑1 (MQ=255)
                                                                                                                  aCGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGc                                    >  1:782243/1‑149 (MQ=255)
                                                                                                                  aCGTCTCTCGCGTGAAGTAATGCAGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGc                                                                           >  3:103732/1‑110 (MQ=255)
                                                                                                                  aCGTCTCTCGCGTGAAGTAATGCAGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGc                                                                           <  4:103732/110‑1 (MQ=255)
                                                                                                                    gTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGa                                  >  2:586512/1‑149 (MQ=255)
                                                                                                                     tctctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGaa                                 <  4:14119/149‑1 (MQ=255)
                                                                                                                         tcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACa                             >  1:514886/1‑149 (MQ=255)
                                                                                                                           gcgTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATc                           >  2:377315/1‑149 (MQ=255)
                                                                                                                             gTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCaa                         >  4:181017/1‑149 (MQ=255)
                                                                                                                              tGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCaaa                        <  4:420717/149‑1 (MQ=255)
                                                                                                                              tGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCaaa                        <  1:586512/149‑1 (MQ=255)
                                                                                                                              tGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCGAGCGGtgttaca                                    <  4:172426/137‑7 (MQ=255)
                                                                                                                              tGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCGAGCGGtgttaca                                    >  3:172426/1‑131 (MQ=255)
                                                                                                                              tGAAGTAATGAGGTTGTGGTGCCGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGGTGATGAAACGCCTTATCGTTCTTGTTTTGTTTGCCAGCACGCTGCTCACGGGTTGTAACACCGCTCGCGGTTTCGGCCAAGACATCaaa                        <  4:326298/149‑1 (MQ=255)
                                                                                                                               gAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTc                                                                                                                      >  2:72031/1‑54 (MQ=255)
                                                                                                                               gAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCa                                                                             >  3:413721/1‑95 (MQ=255)
                                                                                                                               gAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAAc                       <  2:846037/149‑1 (MQ=255)
                                                                                                                                  gTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACAtc                    >  2:181951/1‑149 (MQ=255)
                                                                                                                                   tAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACAtct                   >  2:1079469/1‑149 (MQ=255)
                                                                                                                                             gtggtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTcc         <  1:1065696/149‑1 (MQ=255)
                                                                                                                                                 tgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGcc                                                                                                     >  4:571124/1‑53 (MQ=255)
                                                                                                                                                    ggCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCAtctctc  >  1:1056971/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATACCGCAGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTC  >  NC_000913/4376132‑4376428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: