Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,376,291 T→C 5.2% intergenic (+26/‑26) efp → / → ecnA polyproline‑specific translation elongation factor EF‑P/entericidin A membrane lipoprotein, antidote entericidin B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,376,2910TC 5.2% 451.9 / 8.8 134intergenic (+26/‑26)efp/ecnApolyproline‑specific translation elongation factor EF‑P/entericidin A membrane lipoprotein, antidote entericidin B
Reads supporting (aligned to +/- strand):  ref base T (59/68);  new base C (2/5);  total (61/73)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 134 total aligned reads displayed.

CTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGC  >  NC_000913/4376144‑4376435
                                                                                                                                                   |                                                                                                                                                
cTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTg                                                                                                                                                 <  1:1104463/149‑1 (MQ=255)
  ggtggCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCa                                                                                                                                               <  3:54844/149‑1 (MQ=255)
  ggtggCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCa                                                                                                                                               <  2:588178/149‑1 (MQ=255)
   gtggCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAGATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCAc                                                                                                                                              <  2:684353/149‑1 (MQ=255)
   gtggCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCAc                                                                                                                                              >  1:995744/1‑149 (MQ=255)
     ggCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCa                                                                                                                                            >  1:1067840/1‑149 (MQ=255)
       cAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATc                                                                                                                                          <  3:314534/149‑1 (MQ=255)
        aaaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                                                                         >  1:101487/1‑149 (MQ=255)
        aaaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                                                                         >  1:913312/1‑149 (MQ=255)
        aaaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                                                                         >  1:570998/1‑149 (MQ=255)
         aaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCAc                                                                                                                                        >  2:857186/1‑149 (MQ=255)
              gCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTAtt                                                                                                                                   <  1:191851/149‑1 (MQ=255)
                tACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCa                                                                                                                                 >  3:700795/1‑149 (MQ=255)
                 aCCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAg                                                                                                                                <  4:853667/149‑1 (MQ=255)
                 aCCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAg                                                                                                                                <  2:826694/149‑1 (MQ=255)
                   ccTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGt                                                                                                                              <  2:598883/149‑1 (MQ=255)
                    cTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTAtt                                                                                                                                   >  4:502687/1‑143 (MQ=255)
                      gTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAATGCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCag                                                                                                                           <  2:771914/149‑1 (MQ=255)
                      gTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCag                                                                                                                           >  2:249731/1‑149 (MQ=255)
                           cTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgatg                                                                                                                      <  1:831617/149‑1 (MQ=255)
                            tGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgatga                                                                                                                     <  2:569638/149‑1 (MQ=255)
                            tGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgatga                                                                                                                     <  1:1126308/149‑1 (MQ=255)
                             ggCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgatgat                                                                                                                    >  2:1018907/1‑149 (MQ=255)
                              gcgcTGTGGTTAAAGTGCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGGTATTCGATCTCGTGAATAAGTCTCTCGCGTGAAGTAATACGGTTATGGTGCGGCCTGCAGGCTGCACCATCACTGATTTACGTCTgagacatcg                                                                                                                   >  2:149675/1‑144 (MQ=255)
                                    tgGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGc                                                                                                             <  2:824731/149‑1 (MQ=255)
                                     ggTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGcc                                                                                                            <  4:76202/149‑1 (MQ=255)
                                       ttAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCtt                                                                                                          >  3:591686/1‑149 (MQ=255)
                                             ttCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGtt                                                                                                    >  3:862186/1‑149 (MQ=255)
                                               ccGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCt                                                                                                  <  3:319458/149‑1 (MQ=255)
                                               ccGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATAGTTCt                                                                                                  <  4:1051374/149‑1 (MQ=255)
                                                cGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCtt                                                                                                 <  1:1133982/149‑1 (MQ=255)
                                                 gCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTg                                                                                                >  2:400703/1‑149 (MQ=255)
                                                   tGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGtt                                                                                              <  2:240754/149‑1 (MQ=255)
                                                    gTTTGTACACATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGtct                                                                                             <  2:913312/149‑3 (MQ=255)
                                                    gTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTATGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGttt                                                                                             <  4:964001/149‑1 (MQ=255)
                                                        gTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATc                                                                                                                                          >  4:771267/1‑100 (MQ=255)
                                                        gTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATc                                                                                                                                          <  3:771267/100‑1 (MQ=255)
                                                         tACCAATCCGCGGAGTCATCAAAGTGGATTCCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgctt                                                                                        <  4:591686/149‑1 (MQ=255)
                                                          aCAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttg                                                                                       >  3:420717/1‑149 (MQ=255)
                                                          aCAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttg                                                                                       >  1:350915/1‑149 (MQ=255)
                                                           cAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttgc                                                                                      <  2:350915/149‑1 (MQ=255)
                                                            aaaTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGcc                                                                                     <  1:400703/149‑1 (MQ=255)
                                                              aTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATc                                                                                                                                          <  3:613581/94‑1 (MQ=255)
                                                              aTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATc                                                                                                                                          >  4:613581/1‑94 (MQ=255)
                                                                   cGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACtt                                                                                                                                      <  1:837908/93‑1 (MQ=255)
                                                                     aaGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgctg                                                                            <  4:924593/149‑1 (MQ=255)
                                                                       gTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGtttt                                                                                            >  1:1043603/1‑131 (MQ=255)
                                                                       gTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGtttt                                                                                            <  2:1043603/131‑1 (MQ=255)
                                                                        tcatcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTc                                                                         >  4:123002/1‑149 (MQ=255)
                                                                         catcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCa                                                                        <  2:1133129/149‑1 (MQ=255)
                                                                           tcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgct                                                                                         <  4:649518/130‑1 (MQ=255)
                                                                           tcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgct                                                                                         >  3:649518/1‑130 (MQ=255)
                                                                            caAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACgg                                                                     <  3:896545/149‑1 (MQ=255)
                                                                              aaGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGtt                                                                                              >  4:891170/1‑122 (MQ=255)
                                                                              aaGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGtt                                                                                              <  3:891170/122‑1 (MQ=255)
                                                                               aGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCt                                                                  <  1:1018907/149‑1 (MQ=255)
                                                                               aGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCt                                                                  <  2:995744/149‑1 (MQ=255)
                                                                                      aCCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgc                                                                                          >  1:538195/1‑118 (MQ=255)
                                                                                      aCCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgc                                                                                          <  2:538195/118‑1 (MQ=255)
                                                                                      aCCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAacac                                                           <  4:700795/149‑1 (MQ=255)
                                                                                       cccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCt                                                                  <  3:455747/141‑1 (MQ=255)
                                                                                       cccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAacac                                                           <  3:292970/148‑1 (MQ=255)
                                                                                        ccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATc                                                                                                                                          >  4:675307/1‑68 (MQ=255)
                                                                                        ccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCtt                                                                                                          >  2:193716/1‑100 (MQ=255)
                                                                                        ccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCtt                                                                                                          <  1:193716/100‑1 (MQ=255)
                                                                                        ccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCg                                                         >  4:183645/1‑149 (MQ=255)
                                                                                        ccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCg                                                         >  1:625502/1‑149 (MQ=255)
                                                                                        ccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCTGCACCATc                                                                                                                                          <  3:675307/68‑1 (MQ=255)
                                                                                                      aCGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGc                                           >  1:782243/1‑149 (MQ=255)
                                                                                                      aCGTCTCTCGCGTGAAGTAATGCAGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGc                                                                                  >  3:103732/1‑110 (MQ=255)
                                                                                                      aCGTCTCTCGCGTGAAGTAATGCAGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGc                                                                                  <  4:103732/110‑1 (MQ=255)
                                                                                                        gTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGa                                         >  1:846037/1‑149 (MQ=255)
                                                                                                        gTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGa                                         >  2:586512/1‑149 (MQ=255)
                                                                                                          ctctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAg                                       >  2:1065696/1‑149 (MQ=255)
                                                                                                             tcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACa                                    >  1:1126068/1‑149 (MQ=255)
                                                                                                             tcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACa                                    >  1:514886/1‑149 (MQ=255)
                                                                                                              cgcgTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACAt                                   <  4:862186/149‑1 (MQ=255)
                                                                                                               gcgTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATc                                  >  2:377315/1‑149 (MQ=255)
                                                                                                                 gTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCaa                                >  4:181017/1‑149 (MQ=255)
                                                                                                                  tGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCaaa                               <  4:420717/149‑1 (MQ=255)
                                                                                                                  tGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCGAGCGGtgttaca                                           <  4:172426/137‑7 (MQ=255)
                                                                                                                  tGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCGAGCGGtgttaca                                           >  3:172426/1‑131 (MQ=255)
                                                                                                                  tGAAGTAATGAGGTTGTGGTGCCGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGGTGATGAAACGCCTTATCGTTCTTGTTTTGTTTGCCAGCACGCTGCTCACGGGTTGTAACACCGCTCGCGGTTTCGGCCAAGACATCaaa                               <  4:326298/149‑1 (MQ=255)
                                                                                                                   gAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTc                                                                                                                             >  2:72031/1‑54 (MQ=255)
                                                                                                                   gAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTc                                                                                                                             <  1:72031/54‑1 (MQ=255)
                                                                                                                   gAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCa                                                                                    <  4:413721/95‑1 (MQ=255)
                                                                                                                   gAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCa                                                                                    >  3:413721/1‑95 (MQ=255)
                                                                                                                   gAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAAc                              <  2:846037/149‑1 (MQ=255)
                                                                                                                      gTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACAtc                           <  2:514886/149‑1 (MQ=255)
                                                                                                                       tAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACAtct                          >  2:1079469/1‑149 (MQ=255)
                                                                                                                       tAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACAtct                          >  2:872266/1‑149 (MQ=255)
                                                                                                                                 gtggtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTcc                <  1:1065696/149‑1 (MQ=255)
                                                                                                                                  tggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGAc                                     >  4:1130728/1‑127 (MQ=255)
                                                                                                                                  tggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGAc                                     <  3:1130728/127‑1 (MQ=255)
                                                                                                                                    gtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCAtc             <  1:377315/149‑1 (MQ=255)
                                                                                                                                     tgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGcc                                                                                                            >  4:571124/1‑53 (MQ=255)
                                                                                                                                     tgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGcc                                                                                                            <  3:571124/53‑1 (MQ=255)
                                                                                                                                        ggCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCAtctctc         >  1:1056971/1‑149 (MQ=255)
                                                                                                                                           cTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTcgcg      >  3:1132900/1‑149 (MQ=255)
                                                                                                                                              cAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTg   >  1:94105/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCGCGCTGC  >  NC_000913/4376144‑4376435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: