Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,450,955 A→G 7.1% intergenic (+37/‑103) ytfQ → / → ytfR galactofuranose ABC transporter periplasmic binding protein/putative sugar ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,450,9550AG 7.1% 226.9 / 6.2 70intergenic (+37/‑103)ytfQ/ytfRgalactofuranose ABC transporter periplasmic binding protein/putative sugar ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (32/33);  new base G (3/2);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

TACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTA  >  NC_000913/4450808‑4451102
                                                                                                                                                   |                                                                                                                                                   
tACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATaa                                                                                                                                                    <  1:106180/149‑1 (MQ=255)
 aCAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAg                                                                                                                                                   <  4:943361/149‑1 (MQ=255)
  cAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAgg                                                                                                                                                  <  3:918510/149‑1 (MQ=255)
   aaaaaaGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGgc                                                                                                                                                 <  3:159016/149‑1 (MQ=255)
   aaaaaaGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGgc                                                                                                                                                 <  1:754177/149‑1 (MQ=255)
   aaaaaaGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGgc                                                                                                                                                 <  2:646569/149‑1 (MQ=255)
     aaaaGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGgcgc                                                                                                                                               <  4:1079540/149‑1 (MQ=255)
      aaaGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCt                                                                                                                                              >  4:687798/1‑149 (MQ=255)
         gACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTcgc                                                                                                                                           >  1:355287/1‑149 (MQ=255)
         gACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTcgc                                                                                                                                           >  3:536421/1‑149 (MQ=255)
          aCGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTcgcg                                                                                                                                          >  1:121372/1‑149 (MQ=255)
             gCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGcgccg                                                                                                                                       >  3:318015/1‑149 (MQ=255)
               aCCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATGCGGCGCGAGcgcctta                                                                                                                                     >  3:784313/1‑146 (MQ=255)
                     ccTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATGCGGCGCGAGCGCCTTATCCGGc                                                                                                                               <  4:784313/149‑1 (MQ=255)
                     ccTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGc                                                                                                                               <  2:355287/149‑1 (MQ=255)
                     ccTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGc                                                                                                                               <  4:536421/149‑1 (MQ=255)
                              cTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCa                                                                                                                      >  2:1000442/1‑149 (MQ=255)
                                   gTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGaa                                                                                                                 <  3:249307/149‑1 (MQ=255)
                                       aCCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCt                                                                                                             <  1:1000442/149‑1 (MQ=255)
                                       aCCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCt                                                                                                             >  4:179070/1‑149 (MQ=255)
                                        ccAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGc                                                                                                               <  3:1124365/146‑1 (MQ=255)
                                        ccAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGc                                                                                                               >  4:1124365/1‑146 (MQ=255)
                                        ccAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTg                                                                                                            >  1:1140418/1‑149 (MQ=255)
                                            aTCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGc                                                                                                        >  1:507125/1‑149 (MQ=255)
                                                aCCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACg                                                                                                    >  3:198016/1‑149 (MQ=255)
                                                    tttACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTa                                                                                                >  3:361233/1‑149 (MQ=255)
                                                        cctgcctgATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATGCGGCGCGAGCGCCTTATCCgg                                                                                                                                <  1:807223/113‑1 (MQ=38)
                                                        cctgcctgATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATGCGGCGCGAGCGCCTTATCCgg                                                                                                                                >  2:807223/1‑113 (MQ=38)
                                                        cctgcctgATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgc                                                                                            >  3:785191/1‑149 (MQ=255)
                                                            cctgATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCt                                                                                        <  3:687798/149‑1 (MQ=255)
                                                                  aCCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCAt                                                                                  <  4:925921/149‑1 (MQ=255)
                                                                    cGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTg                                                                                                            <  4:406190/121‑1 (MQ=255)
                                                                    cGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTg                                                                                                            >  3:406190/1‑121 (MQ=255)
                                                                     gCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGcc                                                                               <  4:198016/149‑1 (MQ=255)
                                                                          agaagaATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTg                                                                          <  2:539615/149‑1 (MQ=255)
                                                                           gaagaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTgg                                                                         >  1:660198/1‑149 (MQ=255)
                                                                             agaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATgg                                                                                                                          <  1:644166/98‑1 (MQ=255)
                                                                             agaaTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATgg                                                                                                                          >  2:644166/1‑98 (MQ=255)
                                                                                   agagAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGc                                                                 <  2:660198/149‑1 (MQ=255)
                                                                                         gaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTaca                                                           >  3:354879/1‑149 (MQ=255)
                                                                                            gaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGAt                                                                                                          <  3:227467/99‑1 (MQ=255)
                                                                                            gaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGAt                                                                                                          >  4:227467/1‑99 (MQ=255)
                                                                                             aaaaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCgg                                                       <  3:179070/149‑1 (MQ=255)
                                                                                              aaaaTATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCggg                                                      <  2:507125/149‑1 (MQ=255)
                                                                                                 atatGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGa                                                   >  3:405107/1‑149 (MQ=255)
                                                                                                    tGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAAc                                                >  3:416071/1‑149 (MQ=255)
                                                                                                     ggggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAAcc                                               <  1:893678/149‑1 (MQ=255)
                                                                                                      gggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCa                                              <  2:121372/149‑1 (MQ=255)
                                                                                                        gTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATg                                            <  4:785191/149‑1 (MQ=255)
                                                                                                        gTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATg                                            >  2:353087/1‑149 (MQ=255)
                                                                                                           ttGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATgact                                         >  3:98989/1‑148 (MQ=255)
                                                                                                             gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATc                                                                                                                                   >  3:345208/1‑57 (MQ=255)
                                                                                                             gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATc                                                                                                                                   <  4:345208/57‑1 (MQ=255)
                                                                                                                ggTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgc                                                                                            >  2:757041/1‑93 (MQ=255)
                                                                                                                ggTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgc                                                                                            <  1:757041/93‑1 (MQ=255)
                                                                                                                     cTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAac                               >  3:982331/1‑149 (MQ=255)
                                                                                                                      tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCggg                                                      >  3:40540/1‑125 (MQ=255)
                                                                                                                      tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCggg                                                      <  4:40540/125‑1 (MQ=255)
                                                                                                                        tGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGAt                                                                                                          >  3:726440/1‑71 (MQ=255)
                                                                                                                        tGCCTGATGCCGATTCGTAGGCCGGATAAGCCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGAt                                                                                                          <  4:726440/71‑1 (MQ=255)
                                                                                                                         gCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCa                           >  2:1013316/1‑149 (MQ=255)
                                                                                                                                    aTTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACTAACACCAGGAGAtcctcc                <  4:318015/149‑1 (MQ=255)
                                                                                                                                    aTTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGGGAtcctcc                <  4:98989/149‑1 (MQ=255)
                                                                                                                                    aTTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGAtcctcc                >  2:692598/1‑149 (MQ=255)
                                                                                                                                         tAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCATACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTTCggtgc           >  4:912432/1‑145 (MQ=255)
                                                                                                                                           ggCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGa         >  1:499375/1‑149 (MQ=255)
                                                                                                                                            gCCGGATGCGGCGCGAGCGCCTTATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGaa        >  2:734678/1‑149 (MQ=255)
                                                                                                                                             ccGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAg       <  4:416071/149‑1 (MQ=255)
                                                                                                                                             ccGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAg       <  4:361233/149‑1 (MQ=255)
                                                                                                                                                 aTAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGAtt   <  2:499375/149‑1 (MQ=255)
                                                                                                                                                  tAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTa  >  1:635100/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCCTTTACCTGCCTGATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTA  >  NC_000913/4450808‑4451102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: