Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,470,952 T→C 11.6% intergenic (‑39/+34) ridA ← / ← pyrI enamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,470,9520TC11.6% 267.0 / 21.1 94intergenic (‑39/+34)ridA/pyrIenamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit
Reads supporting (aligned to +/- strand):  ref base T (42/41);  new base C (7/4);  total (49/45)
Fisher's exact test for biased strand distribution p-value = 5.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

GGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGG  >  NC_000913/4470804‑4471100
                                                                                                                                                    |                                                                                                                                                    
ggATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCAt                                                                                                                                                      >  4:667434/1‑149 (MQ=255)
      aCCGGGATCTGACCGGAGGTGATGATCATATTGCTCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTTCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAgg                                                                                                                                                <  1:939591/149‑1 (MQ=255)
      aCCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAgg                                                                                                                                                <  1:74454/149‑1 (MQ=255)
        cGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGa                                                                                                                                              >  4:676109/1‑149 (MQ=255)
        cGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGa                                                                                                                                              >  4:872414/1‑149 (MQ=255)
        cGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGa                                                                                                                                              <  1:23758/149‑1 (MQ=255)
         gggATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAg                                                                                                                                             <  4:530859/149‑1 (MQ=255)
           gATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGcc                                                                                                                                           <  4:501557/149‑1 (MQ=255)
                gACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACt                                                                                                                                      >  2:365419/1‑149 (MQ=255)
                   cGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACtttt                                                                                                                                   <  3:676109/149‑1 (MQ=255)
                        gTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTAc                                                                                                                              >  1:887230/1‑149 (MQ=255)
                          gatgatCATATTGCCCAGATCAACGCCTTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTtta                                                                                                                                  <  4:754989/143‑1 (MQ=255)
                          gatgatCATATGGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCAAGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTtta                                                                                                                                  >  3:754989/1‑143 (MQ=255)
                             gatCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAAcc                                                                                                                         >  4:580917/1‑149 (MQ=255)
                                   atTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaatt                                                                                                                   >  4:133159/1‑149 (MQ=255)
                                       cccAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaattaatt                                                                                                               <  2:1006988/149‑1 (MQ=255)
                                        ccAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAgg                                                                                                                                                >  2:458423/1‑115 (MQ=255)
                                        ccAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAgg                                                                                                                                                <  1:458423/115‑1 (MQ=255)
                                        ccAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTg                                                                                                              >  1:76841/1‑149 (MQ=255)
                                        ccAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTg                                                                                                              <  3:193034/149‑1 (MQ=255)
                                        ccAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTg                                                                                                              >  1:173178/1‑149 (MQ=255)
                                        ccAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTg                                                                                                              >  4:1034256/1‑149 (MQ=255)
                                            aTCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGTGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCa                                                                                                          <  4:784150/149‑1 (MQ=255)
                                            aTCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATtata                                                                                                                                                  >  1:153352/1‑109 (MQ=255)
                                            aTCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATtata                                                                                                                                                  <  2:153352/109‑1 (MQ=255)
                                                  gCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACttttt                                                                                                                                  <  1:905666/119‑2 (MQ=38)
                                                  gCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACttttt                                                                                                                                  >  2:905666/1‑118 (MQ=38)
                                                       gTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTtta                                                                                                                                  <  1:656383/114‑1 (MQ=255)
                                                       gTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTtta                                                                                                                                  >  2:656383/1‑114 (MQ=255)
                                                        tACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTAt                                                                                              >  2:445223/1‑149 (MQ=255)
                                                           gTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATggg                                                                                           >  2:563816/1‑149 (MQ=255)
                                                           gTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATggg                                                                                           >  3:584549/1‑149 (MQ=255)
                                                           gTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATggg                                                                                           >  3:805938/1‑149 (MQ=255)
                                                             aaGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGGTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTttat                                                                                                                                 <  1:834350/109‑1 (MQ=255)
                                                             aaGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGGTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTttat                                                                                                                                 >  2:834350/1‑109 (MQ=255)
                                                             aaGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGaa                                                                                         >  2:1139947/1‑149 (MQ=255)
                                                               ggACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGaaaa                                                                                       >  4:458436/1‑149 (MQ=255)
                                                               ggACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGaaaa                                                                                       >  3:1141894/1‑149 (MQ=255)
                                                                   cGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAActct                                                                                   <  3:872414/149‑1 (MQ=255)
                                                                    gATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTAcc                                                                                                                             <  4:506634/106‑1 (MQ=255)
                                                                    gATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTAcc                                                                                                                             >  3:506634/1‑106 (MQ=255)
                                                                      tAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCtttt                                                                                <  2:173178/149‑1 (MQ=255)
                                                                      tAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCtttt                                                                                <  1:563816/149‑1 (MQ=255)
                                                                       aGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTttat                                                                                                                                 >  3:910770/1‑99 (MQ=255)
                                                                       aGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTttat                                                                                                                                 <  4:910770/99‑1 (MQ=255)
                                                                           gCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGc                                                                                                            >  3:537720/1‑116 (MQ=255)
                                                                           gCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGc                                                                                                            <  4:537720/116‑1 (MQ=255)
                                                                           gCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACGTTATGGGAAAACTCTTTTTcaca                                                                           <  4:72241/149‑1 (MQ=255)
                                                                           gCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCttt                                                                                 <  3:1135929/143‑1 (MQ=255)
                                                                           gCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCttt                                                                                 >  4:1135929/1‑143 (MQ=255)
                                                                           gCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTcaca                                                                           <  2:887230/149‑1 (MQ=255)
                                                                                   aTTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGc                                                                   <  1:445223/149‑1 (MQ=255)
                                                                                         cGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGa                                                             <  1:1139947/149‑1 (MQ=255)
                                                                                         cGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGa                                                             <  3:580917/149‑1 (MQ=255)
                                                                                          gTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTcac                                                                            <  1:495829/133‑1 (MQ=255)
                                                                                          gTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTcac                                                                            >  2:495829/1‑133 (MQ=255)
                                                                                               gATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGcacca                                                                                                    >  4:575778/1‑104 (MQ=255)
                                                                                               gATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGcacca                                                                                                    <  3:575778/104‑1 (MQ=255)
                                                                                                 tAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGata                                                     <  3:458436/149‑1 (MQ=255)
                                                                                                     tttGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAt                                                 <  4:1141894/149‑1 (MQ=255)
                                                                                                         cTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgc                                             >  3:763971/1‑149 (MQ=255)
                                                                                                          tCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGATCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCGCCACATTATGGGAAATCTCtttt                                                                                <  4:3404/113‑1 (MQ=255)
                                                                                                          tCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCtttt                                                                                >  3:3404/1‑113 (MQ=255)
                                                                                                           cATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaattaat                                                                                                                <  2:567459/80‑1 (MQ=255)
                                                                                                           cATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaattaat                                                                                                                >  1:567459/1‑80 (MQ=255)
                                                                                                            aTGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAt                                                 <  2:928973/142‑1 (MQ=255)
                                                                                                            aTGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAt                                                 >  1:928973/1‑142 (MQ=255)
                                                                                                            aTGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGcgcg                                          >  3:118509/1‑149 (MQ=255)
                                                                                                              gATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtt                                        <  4:763971/149‑1 (MQ=255)
                                                                                                                    tcCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCa                                  >  3:959318/1‑149 (MQ=255)
                                                                                                                    tcCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCa                                  <  3:133159/149‑1 (MQ=255)
                                                                                                                    tcCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCa                                  <  4:118509/149‑1 (MQ=255)
                                                                                                                       tttATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTTGCGCGTTTTCGCACgg                               >  4:161751/1‑149 (MQ=255)
                                                                                                                       tttATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg                               >  4:96928/1‑149 (MQ=255)
                                                                                                                             aCAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGc                         >  3:35135/1‑149 (MQ=255)
                                                                                                                                 gtaataaaaGTTTGCCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGa                     >  2:500002/6‑149 (MQ=255)
                                                                                                                                 cGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGa                     <  2:76841/149‑1 (MQ=255)
                                                                                                                                  taataaaaGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGAt                    >  3:241141/5‑149 (MQ=39)
                                                                                                                                  taataaaaGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGAt                    >  3:659793/5‑149 (MQ=39)
                                                                                                                                          gTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCgg            >  2:1018230/1‑149 (MQ=255)
                                                                                                                                          gTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCgg            >  3:992215/1‑149 (MQ=255)
                                                                                                                                          gTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCAttt                                                               >  4:406684/1‑98 (MQ=255)
                                                                                                                                          gTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCAttt                                                               <  3:406684/98‑1 (MQ=255)
                                                                                                                                            cTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGtt          <  4:241141/149‑1 (MQ=255)
                                                                                                                                            cTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGtt          <  4:659793/149‑1 (MQ=255)
                                                                                                                                             tGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCAt                                                                 >  2:246566/1‑93 (MQ=38)
                                                                                                                                             tGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCAt                                                                 <  1:246566/93‑1 (MQ=38)
                                                                                                                                             tGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGTCAAAGCTGGATGAAACCGGTTc         <  4:274062/149‑1 (MQ=255)
                                                                                                                                               gCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCgg       >  3:867414/1‑149 (MQ=255)
                                                                                                                                                cTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGa                                                             <  3:444306/94‑1 (MQ=255)
                                                                                                                                                cTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGa                                                             >  4:444306/1‑94 (MQ=255)
                                                                                                                                                cTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGc      >  1:684321/1‑149 (MQ=255)
                                                                                                                                                 tCATTATAGGGGGCCAGACTTTTATTACCAACCACAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCa     >  2:298215/1‑149 (MQ=255)
                                                                                                                                                 tCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTc         >  3:840148/1‑145 (MQ=255)
                                                                                                                                                 tCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTc         <  4:840148/145‑1 (MQ=255)
                                                                                                                                                    ttATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATgg  >  2:421633/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGG  >  NC_000913/4470804‑4471100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: