Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 438,740 T→G 7.4% N480H (AAC→CAC)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913438,7400TG 7.4% 372.6 / 18.0 107N480H (AAC→CAC) dxs1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
Reads supporting (aligned to +/- strand):  ref base T (50/49);  new base G (4/4);  total (54/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

AGCGTGGCGTTCAGCGATTCGGCGACTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATG  >  NC_000913/438592‑438887
                                                                                                                                                    |                                                                                                                                                   
aGCGTGGCGTTCAGCGATTCGGCGACTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGtt                                                                                                                                                     <  2:464832/149‑1 (MQ=255)
    tGGCGTTCAGCGATTCGGCGACTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCa                                                                                                                                                 >  2:1017369/1‑149 (MQ=255)
     ggCGTTCAGCGATTCGGCGACTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCAc                                                                                                                                                >  4:989330/1‑149 (MQ=255)
       cGTTCAGCGATTCGGCGACTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCAcgc                                                                                                                                              <  4:834886/149‑1 (MQ=255)
        gTTCAGCGATTCGGCGACTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCAcgcg                                                                                                                                             >  2:1067770/1‑149 (MQ=255)
        gTTCAGCGATTCGGCGACTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCAcgcg                                                                                                                                             >  4:640242/1‑149 (MQ=255)
        gTTCAGCGATTCGGCGACTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCAcgcg                                                                                                                                             >  4:265711/1‑149 (MQ=255)
              cGATTCGGCGACTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAgc                                                                                                                                       <  3:972111/149‑1 (MQ=255)
               gATTCGGCGACTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAgcg                                                                                                                                      <  3:723694/149‑1 (MQ=255)
                       gACTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTg                                                                                                                              >  2:721310/1‑149 (MQ=255)
                       gACTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTg                                                                                                                              >  4:1056285/1‑149 (MQ=255)
                        aCTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGa                                                                                                                             >  1:308645/1‑149 (MQ=255)
                          tttCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACg                                                                                                                           >  4:175932/1‑149 (MQ=255)
                          tttCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACg                                                                                                                           >  2:680967/1‑149 (MQ=255)
                           ttCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACgg                                                                                                                          <  2:576397/149‑1 (MQ=255)
                              gccgcTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGcc                                                                                                                       >  1:357875/1‑149 (MQ=255)
                              gccgcTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGcc                                                                                                                       >  1:14751/1‑149 (MQ=255)
                                 gcTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATc                                                                                                                    <  2:108777/149‑1 (MQ=255)
                                   ttCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGt                                                                                                                  <  4:205465/149‑1 (MQ=255)
                                   ttCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGt                                                                                                                  <  2:36381/149‑1 (MQ=255)
                                    tCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGtt                                                                                                                 <  4:212577/149‑1 (MQ=255)
                                       ggCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTtata                                                                                                              <  3:265711/149‑1 (MQ=255)
                                          aTCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTg                                                                                                           >  1:1078255/1‑149 (MQ=255)
                                          aTCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTg                                                                                                           >  4:983844/1‑149 (MQ=255)
                                           tCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGa                                                                                                          <  4:1073760/149‑1 (MQ=255)
                                                 tACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCg                                                                                                    <  1:1033113/149‑1 (MQ=255)
                                                   ccAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAAAGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGt                                                                                                  <  1:1017565/149‑1 (MQ=255)
                                                     aaaGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGtat                                                                                                >  3:271825/1‑149 (MQ=255)
                                                       aGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATag                                                                                              <  2:146698/149‑1 (MQ=255)
                                                        gTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATaga                                                                                             <  3:527496/149‑1 (MQ=255)
                                                         ttAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATagag                                                                                            <  1:478199/149‑1 (MQ=255)
                                                              gATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCt                                                                                       >  1:332755/1‑149 (MQ=255)
                                                                 cGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGc                                                                                    <  3:989330/149‑1 (MQ=255)
                                                                   ccAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGa                                                                                  >  2:810317/1‑149 (MQ=255)
                                                                           tcGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGtt                                                                          <  2:1059233/149‑1 (MQ=255)
                                                                           tcGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGtt                                                                          <  1:319621/149‑1 (MQ=255)
                                                                            cGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGttt                                                                         <  2:357875/149‑1 (MQ=255)
                                                                               cACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTg                                                                                                           >  2:668701/1‑112 (MQ=255)
                                                                               cACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTg                                                                                                           <  1:668701/112‑1 (MQ=255)
                                                                                     gCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGtttt                                                                        >  1:183904/1‑141 (MQ=255)
                                                                                     gCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGtttt                                                                        <  2:183904/141‑1 (MQ=255)
                                                                                     gCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTc                                                                <  4:271825/149‑1 (MQ=255)
                                                                                      cTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTGGCAACGCggtc                                                                                                                                          <  2:976804/74‑3 (MQ=255)
                                                                                      cTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTGGCAACGCggtc                                                                                                                                          >  1:976804/1‑72 (MQ=255)
                                                                                       ttCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCAt                                                                     <  4:283983/142‑1 (MQ=255)
                                                                                       ttCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCgg                                                              >  3:233013/1‑149 (MQ=255)
                                                                                       ttCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCgg                                                              <  3:490223/149‑1 (MQ=255)
                                                                                        tCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCggg                                                             <  1:1067770/149‑1 (MQ=255)
                                                                                         cacaATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCgggg                                                            >  4:668792/1‑149 (MQ=255)
                                                                                          acaATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGt                                                           >  4:1040651/1‑149 (MQ=255)
                                                                                          acaATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGt                                                           >  3:347284/1‑149 (MQ=255)
                                                                                          acaATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCACTCGGGGt                                                           <  3:1056285/149‑1 (MQ=255)
                                                                                            aaTGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCa                                                         >  4:325153/1‑149 (MQ=255)
                                                                                              tGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATa                                                       <  4:233013/149‑1 (MQ=255)
                                                                                                 cTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATg                                                    >  2:42222/1‑149 (MQ=255)
                                                                                                  tttGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATaa                                                      >  3:699399/1‑146 (MQ=255)
                                                                                                  tttGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATaa                                                      <  4:699399/146‑1 (MQ=255)
                                                                                                  tttGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGa                                                   <  1:680967/149‑1 (MQ=255)
                                                                                                  tttGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGa                                                   >  1:76875/1‑149 (MQ=255)
                                                                                                   ttGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGAc                                                  <  3:640242/149‑1 (MQ=255)
                                                                                                      ccAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTtat                                                                                                               <  3:359493/85‑1 (MQ=255)
                                                                                                      ccAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTtat                                                                                                               >  4:359493/1‑85 (MQ=255)
                                                                                                      ccAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCAt                                               >  3:965907/1‑149 (MQ=255)
                                                                                                         aTTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTc                                            >  4:102735/1‑149 (MQ=255)
                                                                                                            ggTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCgg                                         <  4:956464/149‑1 (MQ=255)
                                                                                                             gTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCa                                                                      <  3:375259/119‑1 (MQ=255)
                                                                                                             gTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCa                                                                      >  4:375259/1‑119 (MQ=255)
                                                                                                             gTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGt                                        >  1:222802/1‑149 (MQ=255)
                                                                                                             gTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGt                                        <  3:983844/149‑1 (MQ=255)
                                                                                                              tAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTa                                       >  1:714485/1‑149 (MQ=255)
                                                                                                              tAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTa                                       <  3:175932/149‑1 (MQ=255)
                                                                                                                 tttttCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGa                                                                                                                             <  4:1138294/60‑1 (MQ=255)
                                                                                                                 tttttCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGa                                                                                                                             >  3:1138294/1‑60 (MQ=255)
                                                                                                                 tttttCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGc                                    >  2:150360/1‑149 (MQ=255)
                                                                                                                 tttttCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGc                                    >  3:1125569/1‑149 (MQ=255)
                                                                                                                       cAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCa                              <  4:347284/149‑1 (MQ=255)
                                                                                                                        aGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAg                             <  2:14751/149‑1 (MQ=255)
                                                                                                                        aGCCGCGACAGTTCCACCCCGACCGCGTTGCCACGCGGGGAGCACACAGCTGACGGGCCATCGTTATAGTGATAGCTGGTATGGAGCATCAGACGAAATTCGCGTTCATCGATCGGGGCCATAATGACCCTTTCCGGTAGGCAGCGCAg                             >  2:515476/1‑149 (MQ=255)
                                                                                                                         gcggcgTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAgg                            >  3:646239/1‑149 (MQ=255)
                                                                                                                              gTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAaga                       <  4:965907/149‑1 (MQ=255)
                                                                                                                              gTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAaga                       <  2:205677/149‑1 (MQ=255)
                                                                                                                              gTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAaga                       <  2:222802/149‑1 (MQ=255)
                                                                                                                               tCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAagag                      >  1:876046/1‑149 (MQ=255)
                                                                                                                                 aGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAagagag                    >  3:310215/1‑149 (MQ=255)
                                                                                                                                   ttCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGAt                  >  4:702017/1‑149 (MQ=255)
                                                                                                                                       aCGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATaga                                                                                             >  4:934623/1‑70 (MQ=255)
                                                                                                                                        cGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCaaaa             <  4:1125569/149‑1 (MQ=255)
                                                                                                                                        cGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCaaaa             <  1:1017369/149‑1 (MQ=255)
                                                                                                                                          ctacccGCGTGGCAACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGAc                                                  <  2:75389/106‑1 (MQ=255)
                                                                                                                                          ctacccGCGTGGCAACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGAc                                                  >  1:75389/5‑110 (MQ=255)
                                                                                                                                           tacccGCGTGGCAACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGtt                                                                          >  4:257154/4‑85 (MQ=255)
                                                                                                                                           tacccGCGTGGCAACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGtt                                                                          <  3:257154/82‑1 (MQ=255)
                                                                                                                                           cGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCg                                                                                                    <  2:665850/59‑1 (MQ=255)
                                                                                                                                           cGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCg                                                                                                    >  1:665850/1‑59 (MQ=255)
                                                                                                                                            acccGCGTGGCAACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGAt                  <  1:8239/138‑1 (MQ=255)
                                                                                                                                            acccGCGTGGCAACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGAt                  >  2:8239/3‑140 (MQ=255)
                                                                                                                                             aCCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCAcc        >  3:1108355/1‑149 (MQ=255)
                                                                                                                                                gcgTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTg     >  3:146422/1‑149 (MQ=255)
                                                                                                                                                  gTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGAt   <  4:646239/149‑1 (MQ=255)
                                                                                                                                                   ttGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATg  <  3:325153/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGCGTGGCGTTCAGCGATTCGGCGACTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATG  >  NC_000913/438592‑438887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: