Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,3801.C94.9% 168.4 / 3.4 61intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (0/3);  new base C (27/29);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01
*NC_0009134,296,3802.G94.9% 164.5 / 2.4 61intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (0/3);  new base G (27/29);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

GCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTC  >  NC_000913/4296159‑4296645
                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                                                             
gCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTNGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTCCGCCAGCCAGCGCCATCGCCTTATTTAGCGTGA‑TTTTTTAGGTCGGATAAGGCGTTT‑‑ACCC‑C‑GCCTCCGACACCAATGACGTGA‑GCGACGCTTGCCGCGCCTTCTCCTGCCt                                                                                                                                                                                                                        >  4:320716/1‑276 (MQ=11)
            aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ATGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGtt                                                                                                                                                                                                           >  2:237358/1‑276 (MQ=2)
                    aTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTaa                                                                                                                                                                                                     >  1:246963/1‑274 (MQ=9)
                           gcttgccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCTTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACACCAATG‑CCTGATTCGACGCTTCCCGCGCCGTATAAGGCCTATCTTAA‑CCGCTGGTTAAtttttta                                                                                                                                                                                              >  3:238315/7‑272 (MQ=11)
                           gcttgccgcgTCTTATACGGCCTACGCCAGAAACCGCAATGACCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGCTTATGCCGCATCCAACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTGGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCaaa                                                                                                                                                                                            <  2:126879/270‑1 (MQ=11)
                           gcttgccgcgTCTTAGAAGGCCTACGCAAGACAGGGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGGCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCaaa                                                                                                                                                                                            <  2:262914/270‑1 (MQ=11)
                              gtggcggtgtgagcAGGTCTACGCCGGAAAGGGCAATGGTTGGATTGAGTGTGATTTTGGTGGGGGGTTAAGGGGTTTCGGCCGGACACGACATCAACGCCTGATGGGACGGTTAACGCGTCTTATCAGGGTTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑CGCGGCAGCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCaaa                                                                                                                                                                                            <  3:296693/260‑1 (MQ=11)
                                ccgcgTTTTATCAGGCCTACGCCAGACAGCGCAATAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCtt                                                                                                                                                                                       <  2:451021/275‑1 (MQ=255)
                                                cTACGCCCGTAAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGGCGGTTAAGGAGTTTATGCCGCATCCAACATCAACGCCGGATGCGACGCTTAACGCGTCTTGTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                       <  2:235086/276‑1 (MQ=14)
                                                 tACGCCAGACAGCGCAATAGCTTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                       <  1:250845/275‑1 (MQ=255)
                                                 tACGCCAGACAGCGCAATAGCCTGATTTAGCTTTATTTTGTAGGTCGGATAAGGCGTTTCTTCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                       <  1:345464/275‑1 (MQ=255)
                                                  aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                       <  1:31190/274‑1 (MQ=255)
                                                  aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTGGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATAAACGCCTGCTGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGTTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                                                                                                                                       <  1:238839/274‑1 (MQ=255)
                                                        gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCCGTTGCGACTCTTGCCGCGTCCTATCAGGCTTAGTTTAA‑CCCTTAGTTAATTTTTCAACCTCTTTCCCCCCGGTTACAAACCCtgca                                                                                                                                                                >  3:163138/1‑272 (MQ=11)
                                                             cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCACATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTAATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAccg                                                                                                                                                          >  2:143137/1‑274 (MQ=255)
                                                                           ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc                                                                                                                                              <  2:402748/274‑1 (MQ=255)
                                                                               cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACCCTTCACACCCGGTATCAAACCCTTCCATAAACCTCAGTTTCAAcccatg                                                                                                                                         >  3:52555/1‑271 (MQ=255)
                                                                                   atgtggTAGGTCGGATAAGGCGTTTATGCCGCATCTGACATGAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCATACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCa                                                                                                                                       <  1:119078/268‑1 (MQ=255)
                                                                                   aTTTTGTAGGTCGGATAAGGCGTTTATGCTGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGACAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTGGCCGCGCCTGATCAGGCTTATCTTAA‑CCGTTGGTTACTTTTTCAAACTCTTCACACCCGGTCTCAAACCCTTCCATACTGCTCACTTTCAACCac                                                                                                                                           >  3:135763/1‑268 (MQ=17)
                                                                                       tGTAGTTCGGTTAAGGCGTTCATCCCGCACCCGACAGCAACGCCTGATGGGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCt                                                                                                                                 <  3:174796/275‑1 (MQ=18)
                                                                                              cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACACCCCAGGTTCAACCATTTCAGCCCCTTTTTCtt                                                                                                                          >  3:206044/1‑275 (MQ=18)
                                                                                              cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTCGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CAGTTGGTTAATTTTTCAAACCCTTCACACCCGGTATCAAACCCTTCAATACAGCTCAGGTTCAACCAGTCAAGCGCCTTTTGCtt                                                                                                                          >  1:445511/1‑275 (MQ=18)
                                                                                                    aGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGCCCTATCTTAA‑CCGTTGATTAATTTTTCAAACCCTTCACCCCCGGTATCATACCTTTTCATACACCCTAGTTTCACCCAGTTCATCTTCTTTTTCTTGttctt                                                                                                                    >  4:161783/1‑275 (MQ=17)
                                                                                                           tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACAccc                                                                                                                                                                                >  3:432889/1‑208 (MQ=255)
                                                                                                           tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATa                                                                                                             >  2:48995/1‑275 (MQ=255)
                                                                                                           tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑ACTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACATCCGGTATCAAACCCTTCCATACAGCTCAGGTTCCACCCGTGCAGCGCCTTTTCCTTGTTCTTCCCGAt                                                                                                              >  3:138735/1‑274 (MQ=18)
                                                                                                           tATGCCGCATCCGACATCAACGCCTGATGCGACGATTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGTTCTATCTTAA‑CCGATGGTTAATTTTTCAAATTCTTCATACCCGGTTTTAAACCCTTACATACCGCTCAGGTTCAACCAGTGCAGCGCCGTTTTCTTGtcctttctcataa                                                                                                            >  4:53800/1‑268 (MQ=17)
                                                                                                               ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGACTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTa                                                                                                                                                                            <  1:432889/208‑1 (MQ=255)
                                                                                                                 gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTTCAACTCTTCACCCCCGGTATCCATCCCTTCCATACATCCCAGATCACCCCAGTCCAACCCCCTTTCCTTTGTCTTCCCGATTCAACctt                                                                                                      >  4:551808/1‑276 (MQ=14)
                                                                                                                  cATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCACCCGACATCAATG‑CCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCTTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTTTTCTCGATAAAAActt                                                                                                      >  1:274721/1‑275 (MQ=255)
                                                                                                                     ccGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACcttctt                                                                                                   <  2:390491/275‑1 (MQ=255)
                                                                                                                          ctCAACGCTTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATGGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCg                                                                                              <  1:52555/274‑1 (MQ=255)
                                                                                                                             aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt                                                                                                                                                                  <  1:253543/204‑1 (MQ=255)
                                                                                                                             aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt                                                                                                                                                                  >  3:253543/1‑204 (MQ=255)
                                                                                                                                              ttgccgcgTCTTATCAGGCCTACGCAAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc                                                                         <  2:279580/272‑1 (MQ=255)
                                                                                                                                              ttAACGGGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc                                                                         <  2:320716/276‑1 (MQ=255)
                                                                                                                                              ctgcctcttCTTATAAGTCTGACGCCAGACGGCGCAATGTCCTTATTTAGCGTGC‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc                                                                         <  4:267672/268‑1 (MQ=37)
                                                                                                                                               tcatgcgTTTTATATGGCGTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGTGCGGAGAAGGCGGGT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCTACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGcc                                                                         <  4:462169/271‑1 (MQ=37)
                                                                                                                                                 aCGCGTCTTATAAGGCCTACGCCAGACAGCGTAAAGGACTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACTC‑C‑TCGTCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGGATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCa                                                                       <  1:103287/275‑1 (MQ=37)
                                                                                                                                                     gTCTTGTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTTA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGGTGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTAc                                                                   <  2:514242/275‑1 (MQ=255)
                                                                                                                                                      acgtATCAGGACGACGCCAGACAGCGCAATGGCCTGATATAGCGTGA‑TTTAGTGGGTCGGATAAGGCGTTTCCG‑‑C‑C‑GCAACCTACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACt                                                                  <  2:542055/272‑1 (MQ=37)
                                                                                                                                                               gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAAcc                                                        >  2:99900/1‑276 (MQ=255)
                                                                                                                                                                         gacacggcAATATCATGATTTGGC‑TGAGTTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGGCATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTTCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGa                                               <  1:439812/269‑1 (MQ=38)
                                                                                                                                                                              cgcAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCCGTTGGTTAATTTTTCAAAct                                                                                                                                                                                          >  2:513461/1‑132 (MQ=14)
                                                                                                                                                                              cgcAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCCAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCCCCTTTTGCTTGTTCTTCTCGATACAACCTTCTTCACCGTTTAATAACATCATTCCCGCCCAGTACCCGGAATAACCGGTTCTGGAAAttac                                         >  4:262267/1‑274 (MQ=37)
                                                                                                                                                                              cgcAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCTGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACTTTCTTCACCGTTTAAGAACATCATTCCCCCCCAGTACTCGGAATCACCGGCTCGGGTAAttt                                          >  3:439812/1‑274 (MQ=37)
                                                                                                                                                                              cgcAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAcatca                                                                                 >  4:279406/1‑236 (MQ=255)
                                                                                                                                                                              cgcAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGACGCCACTTTTGCTTGTTCTTCCCGATAAACACTTATTACCCGTTTCAGAACATCCTTCACGCCCAGTACACGGAATAACCGGCTCGGCAAAttac                                         >  4:226984/1‑274 (MQ=25)
                                                                                                                                                                                 aaTAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCCGTTGGTTAATTTTTCAAACTCtt                                                                                                                                                                                       <  4:513461/132‑1 (MQ=14)
                                                                                                                                                                                 aaTAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTc                                                                              <  2:279406/236‑1 (MQ=255)
                                                                                                                                                                                     gCCTGATTTAGCGTTA‑GTTTGTAGGTCGGATAAGGCGTTTCCG‑‑C‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTTGTTAATTTTTCAACCTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGc                                    <  1:205082/274‑1 (MQ=255)
                                                                                                                                                                                          aTTTGGCGTAA‑GTTTGTAGGTCGGTTAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCg                                <  1:138735/273‑1 (MQ=255)
                                                                                                                                                                                          aTTTAGCGTGG‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCTTTTAAGCACATCATTCCTGCCAAGTACTGGGAACATCCGGCTCGGGAAGTTACaatgccgccttt                             >  4:227605/1‑265 (MQ=37)
                                                                                                                                                                                            ttAACGTGA‑TTTTGTAGCTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATTCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGGGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTg                            <  2:289351/275‑1 (MQ=255)
                                                                                                                                                                                                cGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCATTGCAGCGCCTTTTGCTTGTTCTTCTCGATAATACCTTCTTCCCCGTTTAAGAACATCATTCCCTCCCCGTACTCGGAATAACCGCTTCGTGAAATTGGAAAGCTGCCTTTGaact                        >  3:287197/1‑273 (MQ=38)
                                                                                                                                                                                                    a‑TTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTCCGTCTGAAATAct                     >  3:146018/1‑273 (MQ=255)
                                                                                                                                                                                                      ttgtGTAGGTCTGATAAGGCATTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTTCTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAgg                  <  2:429176/273‑1 (MQ=255)
                                                                                                                                                                                                       tttGTAAGTCGGATAAGGCGGTT‑‑AAGC‑C‑GCATACGGCATCAAGT‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGACTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCAACGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAgg                  <  1:302672/275‑1 (MQ=255)
                                                                                                                                                                                                            aGGTCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAAGG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGcc             <  1:206044/275‑1 (MQ=255)
                                                                                                                                                                                                               tCGGATAAGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTCCAGCGCCTTTTGCTTGTTCTTCCCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCACGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGAATATCTGCGTTTTAAATACCCGATTGCCTTg          >  1:144288/1‑275 (MQ=38)
                                                                                                                                                                                                                      aGGCGTTT‑‑ACGC‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCAtcgtc  <  2:392463/276‑1 (MQ=255)
                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                                                             
GCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGA‑TTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑C‑GCATCCGACATCAATG‑CCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTC  >  NC_000913/4296159‑4296645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑