Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,685,527 A→G G354G (GGT→GGC fumC ← fumarate hydratase (fumarase C),aerobic Class II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,685,5270AG99.1% 412.3 / NA 114G354G (GGT→GGCfumCfumarate hydratase (fumarase C),aerobic Class II
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (63/49);  total (64/50)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CCCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCT  >  NC_000913/1685260‑1685791
                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                        
ccccatcgtcgtggCCGAGGCCTTTCAGGTCAGACTTTCTTTATGCGCTTTTTTGGCGATCTCCGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAgc                                                                                                                                                                                                                                                                  <  4:137017/264‑1 (MQ=255)
 gccAGCGCACGTGCCGCATGGTGTAAGGTCATCCCTTCTTTATGCGCCTTGTTGGCGATAGCGGCGTCTTTTTCATACCCAATGTGGGTGTTAAGCGCAGTCACAAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTGTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGCTCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAgc                                                                                                                                                                                                                                                                  <  4:141519/274‑1 (MQ=255)
 cccAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGGGTTAAGCGCAGTCACCAGCATCAGAGATTCATTGCGTAATTGATTGATTCGCTCACGATTCGCTTCAATACCCACTGCGCAGTGTTTGTTAAAAACTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAAGTGTGGCTCCACATCTGACGGTCGACGTTCTGTTCAAAGTTGCCGTAAgc                                                                                                                                                                                                                                                                  >  3:228453/1‑275 (MQ=255)
  ccggcgcAAGGGCCGCAGGTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGTTCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAgc                                                                                                                                                                                                                                                                  <  1:12962/271‑1 (MQ=255)
   cAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGTGCTTTTTTGGGGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGTGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAgcgc                                                                                                                                                                                                                                                                <  1:361769/275‑1 (MQ=255)
       gcAAGGGCCGCAGCTTTTCAGGTCAGCCCTCCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGCGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGcccc                                                                                                                                                                                                                                                             <  1:275293/274‑1 (MQ=255)
       gcAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTTAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTTTTAAAACTTTCCATTCCATCCGCCAGCAAGCGCACCGATTGGAGGAAACTGTGGATCACAAATGGACGGAAGACGTCAAGTTCAAAGTTGACGGAAGCGcccc                                                                                                                                                                                                                                                             >  3:123927/1‑274 (MQ=255)
         aaGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCa                                                                                                                                                                                                                                                          <  3:101341/275‑1 (MQ=255)
                gCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGCTCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGa                                                                                                                                                                                                                                                    >  1:19302/1‑274 (MQ=255)
                 cAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGTTTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCTTCTGCCACAAAGCGCACCGATTGAAGGAACTTGGGGATCACCATTGGACGGACAACGTTCAGTTCAAAGTTGCAGGACGCGCCACCCATGTTgcac                                                                                                                                                                                                                                                  >  4:559154/1‑272 (MQ=255)
                    cTTTTAATGTCAGCCCTTCTTAATGCGCTTGTTTGGCGTGCACGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCa                                                                                                                                                                                                                                              <  2:202969/276‑1 (MQ=255)
                    cTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGGGTTAAGCGCAGTCACCATCATCAGCGATTCATTGAGTAATTGATTGATTCGCACACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGc                                                                                                                                                                                                                                                <  2:138736/274‑1 (MQ=255)
                     ttttAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCGGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCa                                                                                                                                                                                                                                              <  1:115458/275‑1 (MQ=255)
                                 cccTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAATCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCAt                                                                                                                                                                                                                                 <  2:37701/276‑1 (MQ=255)
                                     tcttTATGCGCTTTTTTGGCGATCTCGGCGGCTTGGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCAcc                                                                                                                                                                                                                             <  2:505909/276‑1 (MQ=255)
                                            gcgcTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                                                                                                                                                       <  1:382542/275‑1 (MQ=255)
                                                ttttttGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGAGCCCCCATGTTGATCGCTACGTCGTTCCACATCACCTGACACAagag                                                                                                                                                                                                                  >  2:33518/1‑275 (MQ=255)
                                                 tttttGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                                                                                                                                                 <  2:192803/276‑1 (MQ=255)
                                                   tttGGCGAACTCGGCGGCTTTGTCATAACCAATGTGGGTGTTATGCGCAGTCACGAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAAGACCCAATGCGCAGTGTTTGTTAAAACTTTCCATGCAATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCa                                                                                                                                                                                                                <  1:133816/275‑1 (MQ=255)
                                                        ctgtctcGGCGGCTTTGTCATAACCAAAGTATGGTTTTAGCGCAGGCACCATCATTAGTGGTTCATTTGGTTATTGGATTATTTCCTCACGATTTGGTTCCATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGTAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                                                                                                                                          <  2:315166/273‑1 (MQ=255)
                                                        cGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGTCGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                                                                                                                                          <  2:537100/276‑1 (MQ=255)
                                                             tcGGCGGCTTTGTCATAACCAAGGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATACGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                                                                                                                                                                                     <  2:125562/275‑1 (MQ=255)
                                                                 cggcTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcac                                                                                                                                                                                                 <  2:165113/276‑1 (MQ=255)
                                                                  ggcTTGCTCATAACCAGTGTCGCTGTTAAGCGCAGTCACAAGCATCAGCGATTCATTGACTGATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                                                                                                                                                <  2:170108/276‑1 (MQ=255)
                                                                  ggcTTAGTCATAACCAATGTTGGTGTTAAGCGCAGTCACCAGCGTCAGCGAGTCATTGAGTAATTGATTGATTCGCTCACGCTTCGGTTCAATACCCACTGCGCAGTGTGTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAAGTGTGGATCACCATTGGACGGACGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGGCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                                                                                                                                                <  2:436370/276‑1 (MQ=255)
                                                                   gcTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCCATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGGGCATGGTTAATGCCTcaca                                                                                                                                                                                                <  1:123927/275‑1 (MQ=255)
                                                                              aCCAATGTGGGTGTTACGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTACAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGt                                                                                                                                                                                     <  2:210644/275‑1 (MQ=255)
                                                                                 aaTGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTTCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                                                                                                                                          <  4:258129/251‑1 (MQ=255)
                                                                                   tgtgGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCTAGCAAGCGAACCGATCGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCACAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTTCCAATCACCTGACAGCAGAGCATGTTTAATGCCTCACACTCTGTTGGGTTCACt                                                                                                                                                                                >  1:261895/1‑275 (MQ=255)
                                                                                   tgtgGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCCGCAAGCGCCCCGATTGCAGGAAATTGTGGACCCCCATTTGACGGACCCCGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCCCGTTGATCCCCATGTCTTTCCCCATCCCTTCACCGCCGCGCACGGTTAATGCCTCCCCCCTTGTTGCGTTCACtt                                                                                                                                                                               >  4:515027/1‑276 (MQ=255)
                                                                                   tgtgGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGcc                                                                                                                                                                                                                                                               >  1:196782/1‑196 (MQ=255)
                                                                                   tgtgGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCAGGTTGATCGCCACGGCGTTCCCCATCACCTGCCAGCAGAGCATGGTTAACGCCCCCCACTGGGTTGGGTTCACtt                                                                                                                                                                               >  4:244206/1‑276 (MQ=255)
                                                                                   tgtgGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCCCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCCGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAACCGTTCAGTTCAAAGTTTCCGGAAGCGCTCCCCTGTTTGCTCGCCACGGCGTGCCCCATCCCCTGCCAGCAGAGAAGGTTAAAGGACCCACACTGATTGTGGTTCACtt                                                                                                                                                                               >  4:100271/1‑276 (MQ=255)
                                                                                       ggTTTTAAGCGCAGTCACCAGCATAAGCGATTCATTGAGGAATTGTTTGTTTCGCTCACGATTCGGTTCAATTCCCACTGCGCAGTGTTTGTTAAAACTGTCCATGCCATCATCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACGGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTccc                                                                                                                                                                           <  4:201609/276‑1 (MQ=255)
                                                                                       ggTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGcccccc                                                                                                                                                                                                                                                           <  3:196782/196‑1 (MQ=255)
                                                                                             aaGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAgcgc                                                                                                                                                                                                                                                                >  2:103880/1‑185 (MQ=255)
                                                                                             aaGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAgcgc                                                                                                                                                                                                                                                                >  4:518950/1‑185 (MQ=255)
                                                                                             aaGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCa                                                                                                                                                                      >  1:315851/1‑275 (MQ=255)
                                                                                                cgcAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGcccc                                                                                                                                                                                                                                                             <  4:103880/185‑1 (MQ=255)
                                                                                                cgcAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGcccc                                                                                                                                                                                                                                                             <  2:518950/185‑1 (MQ=255)
                                                                                                  cAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGAt                                                                                                                                                                  >  1:281923/1‑274 (MQ=255)
                                                                                                   aGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTGGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGACTGGTTAATGCCTCACCCTGTGTTGGGTTCACTTTCCCCGCCATGATTg                                                                                                                                                                >  1:166592/1‑275 (MQ=255)
                                                                                                    gTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGAGTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCCCCTGACAGCAGAGCATGGTTAATTCCTCACACTGTTTTGGGTTCAATTTCCCCGGCATGAtttc                                                                                                                                                               >  1:402834/1‑273 (MQ=255)
                                                                                                       aCCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGt                                                                                                                                                                                                            <  3:37883/227‑1 (MQ=255)
                                                                                                             aTCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCATTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCACGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCTCCATGTTGATCTCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCATGACACTGTGTTTGGTTCACTTTACTCCGCATGTTCGAGCTGCCCgg                                                                                                                                                      >  1:184784/1‑275 (MQ=255)
                                                                                                                 gCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATCGAGCTGCCCGTCTCa                                                                                                                                                  >  1:62941/1‑275 (MQ=255)
                                                                                                                      tCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGGCAGGCCCAtttt                                                                                                                                              >  1:270576/1‑274 (MQ=255)
                                                                                                                      tCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTc                                                                                                                                             >  1:299934/1‑275 (MQ=255)
                                                                                                                                 ttgattgaTTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTgaga                                                                                                                                  >  2:139328/1‑275 (MQ=255)
                                                                                                                                      tgattCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCCCCTGCCAGCAGCGCATGGTTAATGCCTCACCCTGTGTTGGGTTCACTTTCCACGGCCTGCTTTAGCTGCCCGGCTCATTTTCCGGGCTTGCGATTTCa                                                                                                                             >  4:71547/1‑275 (MQ=255)
                                                                                                                                      tgattCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCAc                                                                                                                            <  2:579817/276‑1 (MQ=255)
                                                                                                                                      tgattCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCCCATTTTCCGGGATGGAGATTTCa                                                                                                                             >  2:150977/1‑275 (MQ=255)
                                                                                                                                      tgattCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCACCCATGTTGATCGCCACGTCGTTCCCCATCACCTg                                                                                                                                                                                                                           >  1:417085/1‑181 (MQ=255)
                                                                                                                                        attCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTcc                                                                                                                                                                                                                                     >  1:278114/1‑169 (MQ=255)
                                                                                                                                        attCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATg                                                                                                                                                                                                              >  3:345632/1‑192 (MQ=255)
                                                                                                                                          tCGCTCACGCTTCAGTTCAATACCCAATGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAATTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                                                                                                                                                       <  3:417085/181‑1 (MQ=255)
                                                                                                                                            gCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCAt                                                                                                                                                                                                                                 <  3:278114/169‑1 (MQ=255)
                                                                                                                                            gCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                                                                                                                                          <  1:345632/192‑1 (MQ=255)
                                                                                                                                                   aTTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAgcg                                                                                                               <  4:139328/276‑1 (MQ=255)
                                                                                                                                                            aaTACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAACGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCCTGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTTAGCTGCCCGGCTCGTTTTCCGGGATTGAGCTTTGACCACTTCCGCACCCGGGGACaga                                                                                                      >  2:274311/1‑276 (MQ=255)
                                                                                                                                                                   aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGcccccc                                                                                                                                                                                                                                                           >  2:157358/1‑120 (MQ=255)
                                                                                                                                                                   aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCCCGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGTCTCATTTTCCGGCATGGAGTTTTCTCACATTACGCCGCCCGCGCCCGAGgccacc                                                                                               >  4:287628/1‑274 (MQ=255)
                                                                                                                                                                   aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATTCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCCTTTTCCGGGTTTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGgccag                                                                                                >  1:423250/1‑275 (MQ=255)
                                                                                                                                                                   aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGgccag                                                                                                >  3:291063/1‑275 (MQ=255)
                                                                                                                                                                   aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCCCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGTTTCAGATTTCACCATTTCCGCAGCCCGGGCCAGTGgcacg                                                                                                >  3:403270/1‑272 (MQ=255)
                                                                                                                                                                      gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATg                                                                                                                                                                                                                                                        <  4:157358/120‑1 (MQ=255)
                                                                                                                                                                      gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCCTTTTCCGGTATTGAGATTTCCCCAATGCCGCAGCGCGGGCCAGAGtcccgctc                                                                                             >  4:361849/1‑273 (MQ=255)
                                                                                                                                                                      gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCATCCCGGGCCCAAGgccagccg                                                                                             >  2:244707/1‑274 (MQ=255)
                                                                                                                                                                      gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGgccaccca                                                                                             >  2:60617/1‑275 (MQ=255)
                                                                                                                                                                      gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCAACAATTCCGCACGCCGGGCCAGAGgccagcgc                                                                                             >  2:171219/1‑273 (MQ=255)
                                                                                                                                                                      gcgcAGTGTTTGTTAAAACTTTCCATGACATCTGCCAGCAAGCGAACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCAGCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGgccagcca                                                                                             <  2:306197/275‑1 (MQ=255)
                                                                                                                                                                       cgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGTTTGAGATTTCACCAATTCCGCCGCGCGGGCCAGAGGccagccag                                                                                            >  4:574216/1‑275 (MQ=255)
                                                                                                                                                                          aGTATTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAAGTGTGGATAACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGa                                                                                        <  2:529272/276‑1 (MQ=255)
                                                                                                                                                                           gtgtTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGAc                                                                                       <  2:71547/276‑1 (MQ=255)
                                                                                                                                                                            tgtttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTAACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGAca                                                                                      >  2:528787/1‑276 (MQ=255)
                                                                                                                                                                                 gttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACttt                                                                                                                                                                              >  2:464702/1‑183 (MQ=255)
                                                                                                                                                                                 gttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCCGAGGCCAGCCAGCGGACATCAtt                                                                                 >  2:46776/1‑276 (MQ=255)
                                                                                                                                                                                 gttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTGCACTTTCCCCGGCATTATTGAGCTGCCCGGCTCATTTTCCGGGATTGTGATTTCACCAATTTCGCCCCGCGGTCCAGAGGCCAGCCAGCGGCcagcct                                                                                  >  3:382742/1‑271 (MQ=255)
                                                                                                                                                                                    aaaaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTccc                                                                                                                                                                           <  4:464702/183‑1 (MQ=255)
                                                                                                                                                                                    aaaaCTTTCCAGACCATCTGCCACCAAGCTCACCGATTGCAGTAAATTGTGAATCACCATTGGACGGAAGACGTACAATCCAAAGTTACCCGAAGCGCCCCCCGTTTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTGCATGCCTCACACTATGTTGTGTTCACTTTCCCCGGCCTGATTGCGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCAGACATCATTgg                                                                               <  3:223800/275‑1 (MQ=255)
                                                                                                                                                                                      aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGc                                                                              >  1:335225/1‑274 (MQ=255)
                                                                                                                                                                                      aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTACGAAGCGCGGGCAAGAGGCAAGCCAGCGGACATCATTGGc                                                                              >  1:390773/1‑274 (MQ=255)
                                                                                                                                                                                         tttCCATCCCAGCGTCCAGCAACCGCACCGATTGCAGGAAATTTGGGTTCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGGTGCTCGCCACGTCGTTCCCCATCACCTGACAGCAGAGAATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGGGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGAtt                                                                          <  3:402834/275‑1 (MQ=255)
                                                                                                                                                                                           tCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGGTTGAGCTTTCACCACTTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACCTCCTTGGCgcgttt                                                                        >  2:246961/1‑270 (MQ=255)
                                                                                                                                                                                           tCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGAcatca                                                                                   >  1:348972/1‑264 (MQ=255)
                                                                                                                                                                                            ccATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGCTCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCATAGCATGGTTACTGCCCCACACTGTGTGTGGTTCACTTTCCCCGGCATGCTTGCGCTGCCCCGCTCATTTTCCGGGATTGCTCTCTCACCACTTCCGCCGCGCCGCCCGGATGCCAGCCAGCGGCCCTCAGTGGTCAtttt                                                                        >  3:244118/1‑274 (MQ=255)
                                                                                                                                                                                            ccATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGACGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGTCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCACCGCGGGCCAGAGGCCAGCCAGGGGACATCCTTGGCGAtttt                                                                        >  1:298868/1‑274 (MQ=255)
                                                                                                                                                                                               tGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTgg                                                                               <  3:348972/264‑1 (MQ=255)
                                                                                                                                                                                                          agacagcgagccGATGGAAGGAAATTGTGGATCGACATTGGACGGGAGACGTTCAGCTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGGTCCCCCTCACCTGACAGCAGAGCATGGTTAATGCCTCACACGGTGTTGGGTTCACTTTCCCCGGCATGATTGGGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGcc                                                        <  2:474279/266‑1 (MQ=255)
                                                                                                                                                                                                           gCAAGGGCACCGATTGCAGGAAATTGTGGATCACCAGTGGACGGAATACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGcc                                                        <  3:112298/275‑1 (MQ=255)
                                                                                                                                                                                                           gCAAGCTCACCGATTGCAGGAAATTGTGGATACCCATTGGGCGGAAGGCGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGcc                                                        <  3:62941/275‑1 (MQ=255)
                                                                                                                                                                                                                  cACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATgg                                                                                                                                                                                                             >  4:166059/1‑119 (MQ=255)
                                                                                                                                                                                                                  cACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTACTGCCTCACACTGTGTTGGGTTCACTTTCTCCGGCATGATTGAGCTCACCGGCTCCTTTTCCGGGATTGCGATTTCACCAATTCCGCAGCGCGCGCCATAGGCCAGCCAGCGGCAATCATTGGACTTTTTAATCCGTGACGCAACCAACCCtt                                                 >  2:297505/1‑275 (MQ=255)
                                                                                                                                                                                                                                        aTCCCCAGTGGACGGAAGGCGTTCAGTCCAAAGGTGCCGGAAGCCCCCCCCATGTTGATCGCCACGTCCTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAAc                           <  4:244707/275‑1 (MQ=255)
                                                                                                                                                                                                                                        aTCACCATTGGACGGAAGCCGTTGAGTTCAAAGGTGCCGGAACCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAAGGCATGGTTAATGCCTCACACTGTGTTGGGTTCCCTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAAc                           <  3:390773/275‑1 (MQ=255)
                                                                                                                                                                                                                                            ccATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCACTTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCCCCCGGCGCCTGAACCAg                        >  1:326949/1‑274 (MQ=255)
                                                                                                                                                                                                                                             cATTGGACGGAAGACGTTCAGTTCAAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGGGCCGCCGCCACCCTTTTCAACCCGCCGTGCCCCTGAACCCgg                       >  3:276584/1‑274 (MQ=255)
                                                                                                                                                                                                                                                             ttcagttcaAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCTCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTTGCGTTTTTCATCAGTGACGCAGCCAACCCTTTCACCTCGCCGTGCGCGTTAACCATGGCAtgctagggcgcccg      >  3:118045/1‑262 (MQ=255)
                                                                                                                                                                                                                                                             ttcagttcaAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGAATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGTCTCATTTTCCGGGATTGAGATTTCACAAATTCAGCAGCGCGGGCAAGAGGCCAGCCAGCGGACATCATTGGCGCTTTTACTGAATGACGACGCCAACACTTTCAACGCGCCGTTCGCCTGAACCAGGTCATAACAGGTCCCCAgc     >  2:165172/1‑276 (MQ=255)
                                                                                                                                                                                                                                                                  ttcaAAGTTGCCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGACCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCt  >  1:326062/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                        
CCCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCTTCTTTATGCGCTTTTTTGGCGATCTCGGCGGCTTTGTCATAACCAATGTGGGTGTTAAGCGCAGTCACCAGCATCAGCGATTCATTGAGTAATTGATTGATTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCT  >  NC_000913/1685260‑1685791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: