Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,987,450:1 +A 8.1% intergenic (+22/+57) ftnB → / ← yecJ ferritin B, putative ferrous iron reservoir/DUF2766 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,987,4501.A 8.1% 336.8 / 28.9 86intergenic (+22/+57)ftnB/yecJferritin B, putative ferrous iron reservoir/DUF2766 family protein
Reads supporting (aligned to +/- strand):  ref base . (36/43);  new base A (4/3);  total (40/46)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01

ACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGG  >  NC_000913/1987302‑1987598
                                                                                                                                                     |                                                                                                                                                    
aCAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCAtt                                                                                                                                                       >  4:562470/1‑149 (MQ=255)
    cagcaTGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTgcgc                                                                                                                                                  <  1:277997/149‑1 (MQ=255)
      gcaTGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCg                                                                                                                                                >  3:611806/1‑149 (MQ=255)
       caTGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGa                                                                                                                                               <  1:72977/149‑1 (MQ=255)
       caTGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGa                                                                                                                                               >  1:73613/1‑149 (MQ=255)
       caTGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCAATGCATTAGCGCCGa                                                                                                                                               <  4:753251/149‑1 (MQ=255)
          gatgGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGa                                                                                                                                            >  4:255991/1‑149 (MQ=255)
          gatgGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCAATGCATTAGCGCCGATGa                                                                                                                                            <  3:238594/149‑1 (MQ=255)
           atgGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGaa                                                                                                                                           <  3:562470/149‑1 (MQ=255)
           atgGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGaa                                                                                                                                           <  1:729035/149‑1 (MQ=255)
           atgGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGaa                                                                                                                                           <  4:364936/149‑1 (MQ=255)
              gTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGt                                                                                                                                        >  3:275500/1‑149 (MQ=255)
                cTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGttt                                                                                                                                      <  4:753830/149‑1 (MQ=255)
                   ttGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTga                                                                                                                                   <  1:85286/149‑1 (MQ=255)
                         cAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAAcc                                                                                                                             >  4:296880/1‑149 (MQ=255)
                           aaCCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCTTCATCGGCGCAATGCATTAGCGCCGATGat                                                                                                                                           >  3:341759/1‑132 (MQ=39)
                           aaCCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCTTCATCGGCGCAATGCATTAGCGCCGATGat                                                                                                                                           <  4:341759/133‑2 (MQ=39)
                           aaCCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCAtt                                                                                                                                                       >  3:412142/1‑122 (MQ=255)
                           aaCCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCAtt                                                                                                                                                       <  4:412142/122‑1 (MQ=255)
                           aaCCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGc                                                                                                                           >  1:111788/1‑149 (MQ=255)
                            aCCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCt                                                                                                                          >  4:333093/1‑149 (MQ=255)
                               aTTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAg                                                                                                                                          >  1:300656/1‑130 (MQ=255)
                               aTTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAg                                                                                                                                          <  2:300656/130‑1 (MQ=255)
                                    tgatgaAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATc                                                                                                                  <  3:113372/149‑1 (MQ=255)
                                        gaAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGtt                                                                                                              >  4:557979/1‑149 (MQ=255)
                                         aaGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGttt                                                                                                             <  3:726688/149‑1 (MQ=255)
                                           gTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGa                                                                                                           <  3:88998/149‑1 (MQ=255)
                                               gcAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAagca                                                                                                       >  1:547215/1‑149 (MQ=255)
                                                cAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAagcag                                                                                                      <  1:70002/149‑1 (MQ=255)
                                                 aGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAagcagc                                                                                                     <  3:557979/149‑1 (MQ=255)
                                                 aGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAagcagc                                                                                                     <  4:24576/149‑1 (MQ=255)
                                                  gTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCg                                                                                                    <  2:292013/149‑1 (MQ=255)
                                                   tGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCgg                                                                                                   >  1:72785/1‑149 (MQ=255)
                                                    gCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAagcag                                                                                                      <  2:66059/145‑1 (MQ=255)
                                                    gCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAagcag                                                                                                      >  1:66059/1‑145 (MQ=255)
                                                       aaaCTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGtttt                                                                                               >  2:590150/1‑149 (MQ=255)
                                                        aaCTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGttttt                                                                                              <  4:611806/149‑1 (MQ=255)
                                                            tGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAAt                                                                                         <  4:680335/149‑1 (MQ=255)
                                                             gCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAAt                                                                                         <  2:111788/149‑1 (MQ=255)
                                                             gCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAAt                                                                                         <  1:864341/149‑1 (MQ=255)
                                                              cGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATg                                                                                        <  2:547215/149‑1 (MQ=255)
                                                                 gTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGa                                                                                     >  3:583769/1‑149 (MQ=255)
                                                                 gTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGa                                                                                     <  3:291216/149‑1 (MQ=255)
                                                                 gTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGa                                                                                     >  4:291216/1‑149 (MQ=255)
                                                                   aTGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGAtt                                                                                   <  3:255991/149‑1 (MQ=255)
                                                                     gtgCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGtt                                                                                                              <  4:402992/120‑1 (MQ=255)
                                                                     gtgCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGtt                                                                                                              >  3:402992/1‑120 (MQ=255)
                                                                     gtgCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCa                                                                                 <  1:650440/149‑1 (MQ=255)
                                                                       gCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCCTCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCAcc                                                                               <  2:526825/149‑1 (MQ=255)
                                                                       gCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGAt                                                                                                                                              <  3:169875/86‑1 (MQ=255)
                                                                       gCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGAt                                                                                                                                              >  4:169875/1‑86 (MQ=255)
                                                                        cccTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCAccc                                                                              <  4:275500/149‑1 (MQ=255)
                                                                         ccTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCt                                                                             >  2:470793/1‑149 (MQ=255)
                                                                            gtgCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCtgtg                                                                          <  3:296880/149‑1 (MQ=255)
                                                                             tgCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTgg                                                                         <  1:374394/149‑1 (MQ=255)
                                                                             tgCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTgg                                                                         >  3:90233/1‑149 (MQ=255)
                                                                             tgCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTgg                                                                         <  1:472248/149‑1 (MQ=255)
                                                                                aGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATgg                                                                                       >  2:370224/1‑132 (MQ=255)
                                                                                aGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATgg                                                                                       <  1:370224/132‑1 (MQ=255)
                                                                                aGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGt                                                                      >  1:264424/1‑149 (MQ=255)
                                                                                      accAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACtt                                                                <  4:583704/149‑1 (MQ=255)
                                                                                      accAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACtt                                                                >  3:690908/1‑149 (MQ=255)
                                                                                      accAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACtt                                                                <  1:466607/149‑1 (MQ=255)
                                                                                         aaCATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAg                                                             <  2:72785/149‑1 (MQ=255)
                                                                                           cATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGtt                                                           <  4:583769/149‑1 (MQ=255)
                                                                                              gTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTggg                                                                        >  2:650389/1‑133 (MQ=255)
                                                                                              gTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTggg                                                                        <  1:650389/133‑1 (MQ=255)
                                                                                               ttCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAAt                                                       >  3:630608/1‑149 (MQ=255)
                                                                                                  tGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTaaa                                                                   <  2:794184/134‑1 (MQ=255)
                                                                                                  tGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTaaa                                                                   >  1:794184/1‑134 (MQ=255)
                                                                                                               acacCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGt                                                                                                               <  1:659010/77‑1 (MQ=255)
                                                                                                               acacCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGt                                                                                                               >  2:659010/1‑77 (MQ=255)
                                                                                                               acacCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCtttt                                       <  1:148963/149‑1 (MQ=255)
                                                                                                               acacCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGGTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGGAAACTTGAGTTCAATAAGCGCGATGGCtttt                                       >  4:462561/1‑149 (MQ=255)
                                                                                                                cacCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCttttt                                      >  4:800619/1‑149 (MQ=255)
                                                                                                                 acCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTg                                     <  1:5862/149‑1 (MQ=255)
                                                                                                                  ccAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTgg                                    <  2:73613/149‑1 (MQ=255)
                                                                                                                             gATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGa                                                              >  4:412613/1‑112 (MQ=255)
                                                                                                                             gATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCACCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGa                                                              <  3:412613/112‑1 (MQ=255)
                                                                                                                                catcatcGGCGCTAATGCATAGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGt                      >  1:700554/1‑149 (MQ=255)
                                                                                                                                 ttcatcGGCGCAATGCATTAGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTg                     >  3:65624/2‑149 (MQ=255)
                                                                                                                                 atcatcGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTg                     >  2:620275/1‑149 (MQ=255)
                                                                                                                                     tcGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGAAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCg                 >  3:854606/1‑149 (MQ=255)
                                                                                                                                     tcGGCGCAATGTATTAGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCAGTGGGGTAATCTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCg                 >  4:595263/1‑149 (MQ=255)
                                                                                                                                      cGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCgg                <  3:121004/149‑1 (MQ=255)
                                                                                                                                      cGGCGCAATGCATTAGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCgg                >  1:427862/1‑149 (MQ=255)
                                                                                                                                        gcgcTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGAATGGTGAGCGGGt              >  4:548411/1‑149 (MQ=255)
                                                                                                                                            tAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTgc          >  3:398142/1‑149 (MQ=255)
                                                                                                                                            tAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTgc          >  3:655838/1‑149 (MQ=255)
                                                                                                                                               tGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGGTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTgcggc       >  3:97962/1‑149 (MQ=255)
                                                                                                                                                   ttGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTgcggcggcg   >  4:223813/1‑149 (MQ=255)
                                                                                                                                                   ttGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTgcggcggcg   >  4:202650/1‑149 (MQ=255)
                                                                                                                                                    tGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGcggcggcgg  <  1:465681/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGG  >  NC_000913/1987302‑1987598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: