Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,536,246 C→A 100% P138Q (CCG→CAG)  crr → glucose‑specific enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,536,2460CA100.0% 321.1 / NA 89P138Q (CCG→CAG) crrglucose‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (39/50);  total (39/50)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATG  >  NC_000913/2536100‑2536390
                                                                                                                                                  |                                                                                                                                                
cGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAgg                                                                                                                                                <  1:721179/149‑1 (MQ=255)
 gTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGgt                                                                                                                                               <  3:138650/149‑1 (MQ=255)
 gTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGgt                                                                                                                                               <  3:164126/149‑1 (MQ=255)
  tCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGgtt                                                                                                                                              <  3:527044/149‑1 (MQ=255)
     aCTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGgttgtt                                                                                                                                           >  1:721141/1‑149 (MQ=255)
     aCTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGgt                                                                                                                                               <  2:628144/145‑1 (MQ=255)
     aCTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGgt                                                                                                                                               >  1:628144/1‑145 (MQ=255)
       ttCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTAt                                                                                                                                         <  4:371264/149‑1 (MQ=255)
        tCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTAtc                                                                                                                                        <  3:437471/149‑1 (MQ=255)
            tATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCa                                                                                                                                    <  2:359265/149‑1 (MQ=255)
             aTCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCaa                                                                                                                                   <  4:127348/149‑1 (MQ=255)
             aTCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCaa                                                                                                                                   <  2:721141/149‑1 (MQ=255)
                   acCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGTTTGTTATCTCCAACATGGa                                                                                                                             <  4:598384/149‑1 (MQ=255)
                    ccGTTGAACTGAAAGGCGGAGGCTTCAAGCGTATTGCTGAAGAAGGTAAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCAAAGTCTACCCTGACTCAGGTTGTTATCTACAACATGGAc                                                                                                                            <  2:194093/149‑1 (MQ=255)
                     cGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACg                                                                                                                           >  4:186328/1‑149 (MQ=255)
                      gTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGa                                                                                                                          >  4:664990/1‑149 (MQ=255)
                      gTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGa                                                                                                                          >  4:57290/1‑149 (MQ=255)
                       ttGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGaa                                                                                                                         >  3:96820/1‑149 (MQ=255)
                        ttaaCTCAAAGGCGAAGGCTTCAATCGTATTGCAGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGTTCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATAGACGaaa                                                                                                                        <  4:496072/147‑1 (MQ=255)
                                           ttCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACt                                                                                                     >  3:358488/1‑149 (MQ=255)
                                                gCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCg                                                                                                >  1:89752/1‑149 (MQ=255)
                                                 cGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCgg                                                                                               <  4:334098/149‑1 (MQ=255)
                                                   tATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTa                                                                                             <  4:96820/149‑1 (MQ=255)
                                                     ttGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGc                                                                                           >  4:288525/1‑149 (MQ=255)
                                                      tGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCg                                                                                          >  2:662070/1‑149 (MQ=255)
                                                             gaagGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGt                                                                                   >  1:52588/1‑149 (MQ=255)
                                                               agGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTgg                                                                                 <  1:231877/149‑1 (MQ=255)
                                                                gggcAGCTCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTggg                                                                                <  2:52588/146‑1 (MQ=255)
                                                                    aGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGaa                                                                            <  1:319387/149‑1 (MQ=255)
                                                                     gcgcGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTgg                                                                                 >  1:857802/1‑143 (MQ=255)
                                                                     gcgcGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTgg                                                                                 <  2:857802/143‑1 (MQ=255)
                                                                         gTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGAt                                                                                                           <  3:323067/113‑1 (MQ=255)
                                                                         gTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGAt                                                                                                           >  4:323067/1‑113 (MQ=255)
                                                                          tGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCaaa                                                                                                       >  2:87532/1‑116 (MQ=255)
                                                                          tGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCaaa                                                                                                       <  1:87532/116‑1 (MQ=255)
                                                                          tGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCg                                                                      >  3:276174/1‑149 (MQ=255)
                                                                           gAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCgg                                                                     >  1:833913/1‑149 (MQ=255)
                                                                           gAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCgg                                                                     <  2:698215/149‑1 (MQ=255)
                                                                           gAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCgg                                                                     <  3:225436/149‑1 (MQ=255)
                                                                           gAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCgg                                                                     >  3:203256/1‑149 (MQ=255)
                                                                           gAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCgg                                                                     <  3:186328/149‑1 (MQ=255)
                                                                               gTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTTCGGTAGCGTAACCGTGGGTGAAACCCCGGTTAt                                                                 >  2:449425/1‑149 (MQ=255)
                                                                               gTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTAt                                                                 >  4:802158/1‑149 (MQ=255)
                                                                                  ggCGATACTGTCGTTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCg                                                              >  2:760879/1‑149 (MQ=255)
                                                                                          tGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGaaa                                                                                                                        <  1:779510/83‑1 (MQ=255)
                                                                                          tGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGaaa                                                                                                                        >  2:779510/1‑83 (MQ=255)
                                                                                           gTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCGACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCaagaa                                                     <  3:817339/149‑1 (MQ=255)
                                                                                           gTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCaaa                                                                                                       >  1:789342/1‑99 (MQ=255)
                                                                                           gTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCaaa                                                                                                       <  2:789342/99‑1 (MQ=255)
                                                                                           gTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCaagaa                                                     <  1:427268/149‑1 (MQ=255)
                                                                                           gTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCaagaa                                                     >  1:593664/1‑149 (MQ=255)
                                                                                           gTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCaagaa                                                     <  1:704031/149‑1 (MQ=255)
                                                                                           gTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCaagaa                                                     <  3:802158/149‑1 (MQ=255)
                                                                                            tCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCaagaag                                                    >  3:773376/1‑149 (MQ=255)
                                                                                            tCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCaagaag                                                    >  2:622376/1‑149 (MQ=255)
                                                                                              aTTGAATTTGATCTGCCGCTGCTGGAAGAGAACGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACAt                                                                                                                                >  3:787496/1‑71 (MQ=255)
                                                                                              aTTGAATTTGATCTGCCGCTGCTGGAAGAGAACGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACAt                                                                                                                                <  4:787496/71‑1 (MQ=255)
                                                                                              aTTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTa                                                  >  3:597529/1‑149 (MQ=255)
                                                                                               ttGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTgg                                                                                 <  1:172215/117‑1 (MQ=255)
                                                                                               ttGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTgg                                                                                 >  2:172215/1‑117 (MQ=255)
                                                                                               ttGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTaa                                                 >  1:475428/1‑149 (MQ=255)
                                                                                                   aTTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCt                                             >  1:183900/1‑149 (MQ=255)
                                                                                                    tttGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCtt                                            >  1:668130/1‑149 (MQ=255)
                                                                                                       gATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGcc                                         >  3:816156/1‑149 (MQ=255)
                                                                                                        aTCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCg                                        >  4:54564/1‑149 (MQ=255)
                                                                                                        aTCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCg                                        <  3:288525/149‑1 (MQ=255)
                                                                                                         tCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGc                                       >  4:51299/1‑149 (MQ=255)
                                                                                                          cTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCa                                      >  3:120963/1‑149 (MQ=255)
                                                                                                           tGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAGGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAg                                     <  2:126122/149‑1 (MQ=255)
                                                                                                           tGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAg                                     <  2:863692/149‑1 (MQ=255)
                                                                                                            gCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGt                                    >  3:190027/1‑149 (MQ=255)
                                                                                                            gCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGt                                    <  3:661796/149‑1 (MQ=255)
                                                                                                                  gctgGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGaaaaa                              <  2:148196/149‑1 (MQ=255)
                                                                                                                   ctgGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAAt                             <  1:617666/149‑1 (MQ=255)
                                                                                                                    tgGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATg                            <  4:634263/149‑1 (MQ=255)
                                                                                                                     ggAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATgg                           <  4:203256/149‑1 (MQ=255)
                                                                                                                     ggAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATgg                           <  2:833913/149‑1 (MQ=255)
                                                                                                                       aaGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGgcg                         <  4:816156/149‑1 (MQ=255)
                                                                                                                           gaAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCa                     >  2:320280/1‑149 (MQ=255)
                                                                                                                                ccAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGgc                <  2:89752/149‑1 (MQ=255)
                                                                                                                                    gTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCa            <  2:366312/149‑1 (MQ=255)
                                                                                                                                     tCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGCACTGCTCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCAt           <  1:112703/149‑1 (MQ=255)
                                                                                                                                     tCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGTAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCAt           >  1:740432/1‑149 (MQ=255)
                                                                                                                                         cccTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCAttttt       >  3:421321/1‑149 (MQ=255)
                                                                                                                                          ccTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCAtttttt      <  4:230204/149‑1 (MQ=255)
                                                                                                                                            tGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTa    <  4:120963/149‑1 (MQ=255)
                                                                                                                                             gACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTAt   <  1:320280/149‑1 (MQ=255)
                                                                                                                                              aCTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATg  <  3:664990/149‑1 (MQ=255)
                                                                                                                                              aCTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATg  <  1:662070/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATG  >  NC_000913/2536100‑2536390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑