Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,972,653 A→T 5.8% intergenic (+16/+16) tas → / ← lplT putative NADP(H)‑dependent aldo‑keto reductase/lysophospholipid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,972,6530AT 5.8% 234.6 / 5.5 69intergenic (+16/+16)tas/lplTputative NADP(H)‑dependent aldo‑keto reductase/lysophospholipid transporter
Reads supporting (aligned to +/- strand):  ref base A (27/38);  new base T (2/2);  total (29/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.06e-01

GCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAA  >  NC_000913/2972507‑2972797
                                                                                                                                                  |                                                                                                                                                
gCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTaaaa                                                                                                                                                <  3:309195/149‑1 (MQ=255)
  aCTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAAcc                                                                                                                                              >  3:671962/1‑149 (MQ=255)
   ctctGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCg                                                                                                                                             <  1:694473/149‑1 (MQ=255)
     ctgctgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGc                                                                                                                                           <  4:310227/149‑1 (MQ=255)
     ctgctgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGc                                                                                                                                           <  4:457353/149‑1 (MQ=255)
         tgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTa                                                                                                                                       >  1:474490/1‑149 (MQ=255)
           ggCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGCGTTaat                                                                                                                                     <  2:187591/149‑2 (MQ=255)
           ggCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTaaa                                                                                                                                     <  4:495921/149‑1 (MQ=255)
                  ccACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAAt                                                                                                                              <  3:492608/149‑1 (MQ=255)
                    aCGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGa                                                                                                                            >  1:825264/1‑149 (MQ=255)
                          gATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACCgcgc                                                                                                                      <  2:825264/149‑1 (MQ=255)
                             cAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGc                                                                                                                   <  3:713617/149‑1 (MQ=255)
                                  gAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACgg                                                                                                                         <  3:703909/138‑1 (MQ=255)
                                  gAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACgg                                                                                                                         >  4:703909/1‑138 (MQ=255)
                                   aaaaCTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATc                                                                                                             <  4:49668/149‑1 (MQ=255)
                                        tAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTaaaa                                                                                                                                                <  2:729276/109‑1 (MQ=255)
                                        tAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTaaaa                                                                                                                                                >  1:729276/1‑109 (MQ=255)
                                         aaCATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAgc                                                                                                       <  3:829612/149‑1 (MQ=255)
                                         aaCATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAga                                                                                                       <  4:746440/149‑2 (MQ=255)
                                            aTCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGcc                                                                                                    <  1:802363/149‑1 (MQ=255)
                                            aTCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGcc                                                                                                    >  4:322032/1‑149 (MQ=255)
                                             tCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGa                                                                                                               >  1:594591/1‑137 (MQ=255)
                                             tCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGa                                                                                                               <  2:594591/137‑1 (MQ=255)
                                             tCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCg                                                                                                   >  4:804633/1‑149 (MQ=255)
                                             tCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCg                                                                                                   >  1:43496/1‑149 (MQ=255)
                                                 aaGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTAt                                                                                               <  1:791356/149‑1 (MQ=255)
                                                  aGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTAtt                                                                                              <  1:16441/149‑1 (MQ=255)
                                                  aGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTAtt                                                                                              <  4:735465/149‑1 (MQ=255)
                                                       gCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAg                                                                                         >  4:732635/1‑149 (MQ=255)
                                                        cATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAgc                                                                                        <  4:588171/149‑1 (MQ=255)
                                                        cATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAgc                                                                                        <  4:543725/149‑1 (MQ=255)
                                                          tCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAgcgc                                                                                      >  2:586186/1‑149 (MQ=255)
                                                          tCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAgcgc                                                                                      >  1:825913/1‑149 (MQ=255)
                                                             ggAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTGAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCaaa                                                                                   <  1:586186/149‑1 (MQ=255)
                                                               aGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACa                                                                                 <  3:50860/149‑1 (MQ=255)
                                                                gTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAg                                                                                >  3:166593/1‑149 (MQ=255)
                                                                 ttAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCACAAACAgc                                                                               >  4:808405/1‑149 (MQ=255)
                                                                       gAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCg                                                                         <  3:732635/149‑1 (MQ=255)
                                                                          gACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAg                                                                      >  2:248410/1‑149 (MQ=255)
                                                                           aCGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgc                                                                     <  2:474490/149‑1 (MQ=255)
                                                                             gTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgcca                                                                   >  2:820644/1‑149 (MQ=255)
                                                                                       aaaTTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATgg                                                         <  4:156129/149‑1 (MQ=255)
                                                                                              aGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGAc                                                  >  3:51009/1‑149 (MQ=255)
                                                                                                cGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCg                                                <  3:804633/149‑1 (MQ=255)
                                                                                                 ggTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCgg                                               <  4:671962/149‑1 (MQ=255)
                                                                                                       tCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGcc                                         <  2:43496/149‑1 (MQ=255)
                                                                                                         aGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTa                                       >  1:439397/1‑149 (MQ=255)
                                                                                                            tttATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCTACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCa                                    >  3:82923/1‑149 (MQ=255)
                                                                                                               ataCTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTa                                 <  1:92431/149‑1 (MQ=255)
                                                                                                               ataCTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTa                                 <  2:439397/149‑1 (MQ=255)
                                                                                                                        cGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCg                        >  4:531523/1‑149 (MQ=255)
                                                                                                                         ggCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGa                       <  2:373701/149‑1 (MQ=255)
                                                                                                                           cACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGt                     <  3:531523/149‑1 (MQ=255)
                                                                                                                            aCCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTa                    >  2:143444/1‑149 (MQ=255)
                                                                                                                              cATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTaaa                  <  1:248410/149‑1 (MQ=255)
                                                                                                                              cATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTaaa                  <  1:820644/149‑1 (MQ=255)
                                                                                                                               aTAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAACCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAAt                 >  1:576981/1‑149 (MQ=255)
                                                                                                                                 aaGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGc               >  2:820209/1‑149 (MQ=255)
                                                                                                                                  aGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGcc              >  3:92126/1‑149 (MQ=255)
                                                                                                                                   gATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGccc             <  2:131989/149‑1 (MQ=255)
                                                                                                                                    tttAACGCCGGTTTTAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCt                                        <  3:638080/120‑1 (MQ=255)
                                                                                                                                    tttAACGCCGGTTTTAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCt                                        >  4:638080/2‑121 (MQ=255)
                                                                                                                                    aTTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATc                                     >  4:296585/1‑124 (MQ=255)
                                                                                                                                    aTTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATc                                     <  3:296585/124‑1 (MQ=255)
                                                                                                                                    aTTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCa            <  4:166593/149‑1 (MQ=255)
                                                                                                                                     ttAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAg           >  1:376717/1‑149 (MQ=255)
                                                                                                                                       aaCGCCGGTTTTAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCa         >  1:853075/1‑149 (MQ=255)
                                                                                                                                        aCGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGc                                          <  2:83685/115‑1 (MQ=255)
                                                                                                                                        aCGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGc                                          >  1:83685/1‑115 (MQ=255)
                                                                                                                                             ggTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATcaaca   >  4:482345/1‑149 (MQ=255)
                                                                                                                                              gTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATcaacaa  <  3:534510/149‑1 (MQ=255)
                                                                                                                                              gTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATcaacaa  <  1:617514/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAA  >  NC_000913/2972507‑2972797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: