Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,098,515 G→C 7.9% intergenic (+112/+43) yggW → / ← yggM HemN family putative oxidoreductase/DUF1202 family putative secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,098,5150GC 7.9% 213.3 / 9.6 63intergenic (+112/+43)yggW/yggMHemN family putative oxidoreductase/DUF1202 family putative secreted protein
Reads supporting (aligned to +/- strand):  ref base G (29/29);  new base C (2/3);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

TTTAAATTCGCTGCTGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACT  >  NC_000913/3098367‑3098662
                                                                                                                                                    |                                                                                                                                                   
tttAAATTCGCTGCTGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCggg                                                                                                                                                     <  1:246495/149‑1 (MQ=255)
   aaaTTCGCTGCTGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTc                                                                                                                                                  >  1:417133/1‑149 (MQ=255)
         gctgctGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAAcgcc                                                                                                                                            >  4:99668/1‑149 (MQ=255)
               ggAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATcc                                                                                                                                      <  1:542889/149‑1 (MQ=255)
                  gCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCAGGATGCGGCGTTGACCCCGCATCCGGc                                                                                                                                   <  2:401137/149‑1 (MQ=25)
                         cTGGCTGAGTAAACTTGTATTGCCGGATGCGGCATGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAAt                                                                                                                            <  4:92191/149‑1 (MQ=255)
                            gCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGt                                                                                                                         <  3:816099/149‑1 (MQ=255)
                            gCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGt                                                                                                                         <  2:417133/149‑1 (MQ=255)
                            gCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGt                                                                                                                         <  4:584729/149‑1 (MQ=255)
                             cTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTg                                                                                                                        <  3:596184/149‑1 (MQ=255)
                              tGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGc                                                                                                                       >  4:584509/1‑149 (MQ=255)
                                 gTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACt                                                                                                                    >  4:555160/1‑149 (MQ=255)
                                  tAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACta                                                                                                                   <  1:309535/149‑1 (MQ=255)
                                   aaaCTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACtat                                                                                                                  <  1:856494/149‑1 (MQ=255)
                                     aCTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTatat                                                                                                                >  3:87070/1‑149 (MQ=255)
                                       ttGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCa                                                                                                              >  3:120098/1‑149 (MQ=255)
                                       ttGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCa                                                                                                              >  2:210708/1‑149 (MQ=255)
                                       ttGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCa                                                                                                              >  3:545214/1‑149 (MQ=255)
                                       ttGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCa                                                                                                              >  4:620443/1‑149 (MQ=255)
                                       ttGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCa                                                                                                              >  4:860147/1‑149 (MQ=255)
                                         gTATTGCCGGATGCGGCGTAAACGCCTTATCCATCCGACCTGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAACCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCaga                                                                                                            <  2:179197/149‑3 (MQ=255)
                                          tATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAac                                                                                                           <  2:823312/149‑1 (MQ=255)
                                              gCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAacttac                                                                                                       >  2:780458/1‑149 (MQ=255)
                                               ccGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAacttact                                                                                                      >  1:441165/1‑149 (MQ=255)
                                                   aTGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAAt                                                                                                  <  3:620443/149‑1 (MQ=255)
                                                     gcggcgTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTACACTATATCAAACTTACTTAATCa                                                                                                <  1:745345/149‑1 (MQ=255)
                                                       ggcgTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCAGGATGCGGCGTTGACCCCGCATCCGGca                                                                                                                                  <  3:104726/113‑2 (MQ=18)
                                                       ggcgTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCAGGATGCGGCGTTGACCCCGCATCCGGca                                                                                                                                  >  4:104726/1‑112 (MQ=18)
                                                        gcgTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCAccc                                                                                             <  4:574887/149‑1 (MQ=255)
                                                        gcgTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCAccc                                                                                             <  1:210708/149‑1 (MQ=255)
                                                             aaCGCCTTATCCAGCCGACACGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTg                                                                                        >  2:100401/1‑149 (MQ=255)
                                                                gCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTgcgc                                                                                     >  4:311505/1‑149 (MQ=255)
                                                                gCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTgcgc                                                                                     >  2:15230/1‑149 (MQ=255)
                                                                    tATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGATGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCa                                                                                 <  4:325630/149‑1 (MQ=255)
                                                                      tCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgt                                                                               >  2:838293/1‑149 (MQ=255)
                                                                         aGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgttgt                                                                            >  3:114698/1‑149 (MQ=255)
                                                                           ccGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTc                                                                          <  2:789107/149‑1 (MQ=255)
                                                                            cGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCg                                                                         <  3:95126/149‑1 (MQ=255)
                                                                             gACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCgg                                                                        >  2:123977/1‑149 (MQ=255)
                                                                               cATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTa                                                                      <  4:120098/149‑1 (MQ=255)
                                                                                  gtgGCAGCGGTAGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCAGCCTGCTACAAATCGTGCACGATATCAATCTTACTTAATCACCCCATTGCGCTGCAGTTGTTCGGTATCt                                                                   <  4:535231/149‑1 (MQ=38)
                                                                                     gCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCtttt                                                                >  3:69101/1‑149 (MQ=255)
                                                                                        gCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTc                                                             >  2:318882/1‑149 (MQ=255)
                                                                                          ggTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCa                                                           <  3:680793/149‑1 (MQ=255)
                                                                                          ggTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCa                                                           <  3:860147/149‑1 (MQ=255)
                                                                                            ttGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTAAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGc                                                         <  2:859479/149‑1 (MQ=255)
                                                                                               tAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGtt                                                      >  2:349948/1‑149 (MQ=255)
                                                                                                   tCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCAtt                                                  >  2:37823/1‑149 (MQ=255)
                                                                                                      gATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGc                                               >  1:651617/1‑149 (MQ=255)
                                                                                                        tAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCtt                                             <  4:87070/149‑1 (MQ=255)
                                                                                                         aaGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTg                                            <  1:783544/149‑1 (MQ=255)
                                                                                                          aGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTgg                                           >  4:696468/1‑149 (MQ=255)
                                                                                                               cgcAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGat                                      <  2:254023/149‑1 (MQ=255)
                                                                                                                       tCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCaa                              >  1:577701/1‑149 (MQ=255)
                                                                                                                            tCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTtc                         >  1:823867/1‑149 (MQ=255)
                                                                                                                             cAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTtca                        >  2:33860/1‑149 (MQ=255)
                                                                                                                              aGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTtcat                       >  1:350582/1‑149 (MQ=255)
                                                                                                                                  gTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATAGTCAATTTTCATCaaa                   >  2:873754/1‑149 (MQ=255)
                                                                                                                                       ttGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATcgc              <  2:609644/149‑1 (MQ=255)
                                                                                                                                         gCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGa            <  3:52605/149‑1 (MQ=255)
                                                                                                                                            ggATGCGGCGTTGACCCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGtt                                         <  4:646869/117‑1 (MQ=255)
                                                                                                                                            ggATGCGGCGTTGACCCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGtt                                         >  3:646869/1‑117 (MQ=255)
                                                                                                                                               tGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACttttt      <  3:99668/149‑1 (MQ=255)
                                                                                                                                               tGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACttttt      <  1:349948/149‑1 (MQ=255)
                                                                                                                                                   gggTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACt  >  3:418672/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTTAAATTCGCTGCTGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACT  >  NC_000913/3098367‑3098662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑