Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,098,522 G→C 7.6% intergenic (+119/+36) yggW → / ← yggM HemN family putative oxidoreductase/DUF1202 family putative secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,098,5220GC 7.6% 220.0 / 6.1 64intergenic (+119/+36)yggW/yggMHemN family putative oxidoreductase/DUF1202 family putative secreted protein
Reads supporting (aligned to +/- strand):  ref base G (29/30);  new base C (2/3);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGCTGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGTTTA  >  NC_000913/3098376‑3098667
                                                                                                                                                  |                                                                                                                                                 
gctgctGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAAcgcc                                                                                                                                                 >  4:99668/1‑149 (MQ=255)
      ggAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATcc                                                                                                                                           <  1:542889/149‑1 (MQ=255)
         gCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCAGGATGCGGCGTTGACCCCGCATCCGGc                                                                                                                                        <  2:401137/149‑1 (MQ=25)
                cTGGCTGAGTAAACTTGTATTGCCGGATGCGGCATGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAAt                                                                                                                                 <  4:92191/149‑1 (MQ=255)
                   gCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGt                                                                                                                              <  4:584729/149‑1 (MQ=255)
                   gCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGt                                                                                                                              <  3:816099/149‑1 (MQ=255)
                   gCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGt                                                                                                                              <  2:417133/149‑1 (MQ=255)
                    cTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTg                                                                                                                             <  3:596184/149‑1 (MQ=255)
                     tGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGc                                                                                                                            >  4:584509/1‑149 (MQ=255)
                        gTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACt                                                                                                                         >  4:555160/1‑149 (MQ=255)
                         tAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACta                                                                                                                        <  1:309535/149‑1 (MQ=255)
                          aaaCTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACtat                                                                                                                       <  1:856494/149‑1 (MQ=255)
                            aCTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTatat                                                                                                                     >  3:87070/1‑149 (MQ=255)
                              ttGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCa                                                                                                                   >  4:860147/1‑149 (MQ=255)
                              ttGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCa                                                                                                                   >  4:620443/1‑149 (MQ=255)
                              ttGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCa                                                                                                                   >  3:120098/1‑149 (MQ=255)
                              ttGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCa                                                                                                                   >  2:210708/1‑149 (MQ=255)
                              ttGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCa                                                                                                                   >  3:545214/1‑149 (MQ=255)
                                gTATTGCCGGATGCGGCGTAAACGCCTTATCCATCCGACCTGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAACCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCaga                                                                                                                 <  2:179197/149‑3 (MQ=255)
                                 tATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAac                                                                                                                <  2:823312/149‑1 (MQ=255)
                                     gCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAacttac                                                                                                            >  2:780458/1‑149 (MQ=255)
                                      ccGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAacttact                                                                                                           >  1:441165/1‑149 (MQ=255)
                                          aTGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAAt                                                                                                       <  3:620443/149‑1 (MQ=255)
                                            gcggcgTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTACACTATATCAAACTTACTTAATCa                                                                                                     <  1:745345/149‑1 (MQ=255)
                                              ggcgTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCAGGATGCGGCGTTGACCCCGCATCCGGca                                                                                                                                       >  4:104726/1‑112 (MQ=18)
                                              ggcgTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCAGGATGCGGCGTTGACCCCGCATCCGGca                                                                                                                                       <  3:104726/113‑2 (MQ=18)
                                               gcgTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCAccc                                                                                                  <  4:574887/149‑1 (MQ=255)
                                               gcgTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCAccc                                                                                                  <  1:210708/149‑1 (MQ=255)
                                                    aaCGCCTTATCCAGCCGACACGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTg                                                                                             >  2:100401/1‑149 (MQ=255)
                                                       gCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTgcgc                                                                                          >  2:15230/1‑149 (MQ=255)
                                                       gCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTgcgc                                                                                          >  4:311505/1‑149 (MQ=255)
                                                           tATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGATGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCa                                                                                      <  4:325630/149‑1 (MQ=255)
                                                             tCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgt                                                                                    >  2:838293/1‑149 (MQ=255)
                                                                aGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgttgt                                                                                 >  3:114698/1‑149 (MQ=255)
                                                                  ccGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTc                                                                               <  2:789107/149‑1 (MQ=255)
                                                                   cGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCg                                                                              <  3:95126/149‑1 (MQ=255)
                                                                    gACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCgg                                                                             >  2:123977/1‑149 (MQ=255)
                                                                      cATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTa                                                                           <  4:120098/149‑1 (MQ=255)
                                                                         gtgGCAGCGGTAGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCAGCCTGCTACAAATCGTGCACGATATCAATCTTACTTAATCACCCCATTGCGCTGCAGTTGTTCGGTATCt                                                                        <  4:535231/149‑1 (MQ=38)
                                                                            gCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCtttt                                                                     >  3:69101/1‑149 (MQ=255)
                                                                               gCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTc                                                                  >  2:318882/1‑149 (MQ=255)
                                                                                 ggTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCa                                                                <  3:860147/149‑1 (MQ=255)
                                                                                 ggTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCa                                                                <  3:680793/149‑1 (MQ=255)
                                                                                   ttGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTAAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGc                                                              <  2:859479/149‑1 (MQ=255)
                                                                                      tAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGtt                                                           >  2:349948/1‑149 (MQ=255)
                                                                                          tCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCAtt                                                       >  2:37823/1‑149 (MQ=255)
                                                                                             gATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGc                                                    >  1:651617/1‑149 (MQ=255)
                                                                                               tAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCtt                                                  <  4:87070/149‑1 (MQ=255)
                                                                                                aaGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTg                                                 <  1:783544/149‑1 (MQ=255)
                                                                                                 aGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTgg                                                >  4:696468/1‑149 (MQ=255)
                                                                                                      cgcAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGat                                           <  2:254023/149‑1 (MQ=255)
                                                                                                              tCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCaa                                   >  1:577701/1‑149 (MQ=255)
                                                                                                                   tCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTtc                              >  1:823867/1‑149 (MQ=255)
                                                                                                                    cAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTtca                             >  2:33860/1‑149 (MQ=255)
                                                                                                                     aGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTtcat                            >  1:350582/1‑149 (MQ=255)
                                                                                                                         gTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATAGTCAATTTTCATCaaa                        >  2:873754/1‑149 (MQ=255)
                                                                                                                              ttGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATcgc                   <  2:609644/149‑1 (MQ=255)
                                                                                                                                gCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGa                 <  3:52605/149‑1 (MQ=255)
                                                                                                                                   ggATGCGGCGTTGACCCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGtt                                              <  4:646869/117‑1 (MQ=255)
                                                                                                                                   ggATGCGGCGTTGACCCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGtt                                              >  3:646869/1‑117 (MQ=255)
                                                                                                                                      tGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACttttt           <  1:349948/149‑1 (MQ=255)
                                                                                                                                      tGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACttttt           <  3:99668/149‑1 (MQ=255)
                                                                                                                                          gggTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACt       >  3:418672/1‑149 (MQ=255)
                                                                                                                                             tCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGtt    <  1:318882/149‑1 (MQ=255)
                                                                                                                                               aaCGCCGCATCCTGCTACAAATCGTGCACTATATAAAACTTACTTAATCACCCCATCGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCATGGTCGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGTTTa  >  4:150497/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCTGCTGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGTTTA  >  NC_000913/3098376‑3098667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑