Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,202,003 G→C 5.6% G38A (GGC→GCC)  cca → fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,202,0030GC 5.6% 373.7 / 9.4 107G38A (GGC→GCC) ccafused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase
Reads supporting (aligned to +/- strand):  ref base G (49/52);  new base C (3/3);  total (52/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 108 total aligned reads displayed.

ATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTACTTGCTATGCCGCACCGGATGTCACGCTGGAAGATGA  >  NC_000913/3201856‑3202150
                                                                                                                                                   |                                                                                                                                                   
attaGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGc                                                                                                                                                    >  3:474180/1‑149 (MQ=255)
 ttaGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCt                                                                                                                                                   >  3:312074/1‑149 (MQ=255)
   aGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTAc                                                                                                                                                 <  4:474180/149‑1 (MQ=255)
        ttGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACcagca                                                                                                                                            <  3:6641/149‑1 (MQ=255)
         tgtgTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACcagcag                                                                                                                                           <  3:191704/149‑1 (MQ=255)
           tgtATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGt                                                                                                                                         >  2:559548/1‑149 (MQ=255)
            gtATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTa                                                                                                                                        <  1:287100/149‑1 (MQ=255)
             tATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAg                                                                                                                                       <  4:224205/149‑1 (MQ=255)
             tATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAg                                                                                                                                       <  4:66337/149‑1 (MQ=255)
              aTTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAga                                                                                                                                      <  3:627276/149‑2 (MQ=255)
               tttGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGc                                                                                                                                     >  4:55787/1‑149 (MQ=255)
               tttGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGCCTACCTGCTGGTAgcc                                                                                                                                     <  1:127585/149‑3 (MQ=255)
                    cGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGa                                                                                                                                <  1:402529/149‑1 (MQ=255)
                    cGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGa                                                                                                                                <  1:743287/149‑1 (MQ=255)
                    cGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGa                                                                                                                                <  1:737819/149‑1 (MQ=255)
                    cGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCAATGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGa                                                                                                                                <  3:557486/149‑1 (MQ=255)
                       aGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGAttt                                                                                                                             <  3:416760/149‑1 (MQ=255)
                          gatgatGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTcc                                                                                                                          <  1:358909/149‑1 (MQ=255)
                           atgatgGCGTGAAGATTTATCTGGTCGGTCGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCt                                                                                                                         <  3:730665/149‑1 (MQ=255)
                             gatgGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGCTGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCtgt                                                                                                                       >  1:712051/1‑149 (MQ=255)
                             gatgGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCtgt                                                                                                                       <  2:118304/149‑1 (MQ=255)
                               tgGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTgtgt                                                                                                                     <  2:78071/149‑1 (MQ=255)
                               tgGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGGCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTgtgt                                                                                                                     >  4:691376/1‑149 (MQ=255)
                               tgGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGATACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTgtgt                                                                                                                     >  2:258272/1‑149 (MQ=255)
                                  cGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTc                                                                                                                  <  4:497390/149‑1 (MQ=255)
                                   gTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCt                                                                                                                 <  1:643895/149‑1 (MQ=255)
                                   gTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCt                                                                                                                 >  3:526078/1‑149 (MQ=255)
                                   gTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCt                                                                                                                 >  2:250124/1‑149 (MQ=255)
                                     gAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGc                                                                                                               >  3:116846/1‑149 (MQ=255)
                                     gAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGc                                                                                                               >  2:746337/1‑149 (MQ=255)
                                          tttATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCg                                                                                                          >  1:87808/1‑149 (MQ=255)
                                           ttATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGc                                                                                                         >  3:646250/1‑149 (MQ=255)
                                              tCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCaaa                                                                                                      >  1:32181/1‑149 (MQ=255)
                                                tGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTATGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACg                                                                                                    >  3:181085/1‑149 (MQ=255)
                                                tGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACg                                                                                                    >  1:133523/1‑149 (MQ=255)
                                                  gTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCa                                                                                                  <  2:32181/149‑1 (MQ=255)
                                                        ggtgCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCATACGCATGAaga                                                                                            <  3:691376/149‑1 (MQ=255)
                                                        ggtgCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAaga                                                                                            <  1:746337/149‑1 (MQ=255)
                                                         gtgCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAagag                                                                                           >  2:660214/1‑149 (MQ=255)
                                                           gCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGCCAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTa                                                                                         <  2:363214/149‑1 (MQ=255)
                                                                   ggATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACcagcag                                                                                                                                           >  2:790981/1‑91 (MQ=255)
                                                                   ggATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACcagcag                                                                                                                                           <  1:790981/91‑1 (MQ=255)
                                                                   ggATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTgg                                                                                 >  2:241417/1‑149 (MQ=255)
                                                                    gATGCATTGTTAGGGCTACCGGTCAAAGACAGAGCTTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGTTTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTgtc                                                                                >  2:84387/1‑147 (MQ=255)
                                                                    gATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTggt                                                                                >  4:629077/1‑148 (MQ=255)
                                                                    gATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGc                                                                                <  4:526078/149‑1 (MQ=255)
                                                                     aTGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCa                                                                               >  3:361369/1‑149 (MQ=255)
                                                                        cATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGt                                                                            >  1:221982/1‑149 (MQ=255)
                                                                         aTTGTTATGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGTCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGAGTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTa                                                                           >  4:732689/1‑149 (MQ=255)
                                                                         aTTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTa                                                                           >  1:47301/1‑149 (MQ=255)
                                                                               aGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAAc                                                                     >  3:69932/1‑149 (MQ=255)
                                                                                    tACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGaaa                                                                <  4:211721/149‑1 (MQ=255)
                                                                                      ccGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCaaa                                                                                                      <  4:212853/109‑1 (MQ=255)
                                                                                      ccGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCaaa                                                                                                      >  3:212853/1‑109 (MQ=255)
                                                                                      ccGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtc                                                              <  4:69932/149‑1 (MQ=255)
                                                                                        ggTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccg                                                            <  3:575260/149‑1 (MQ=255)
                                                                                         gTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCGCAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccgg                                                           <  2:58671/149‑1 (MQ=255)
                                                                                          tCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCAc                                                                              >  4:115970/1‑129 (MQ=255)
                                                                                          tCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCAc                                                                              <  3:115970/129‑1 (MQ=255)
                                                                                           cAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggtt                                                         <  2:105154/149‑1 (MQ=255)
                                                                                            aaaGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggttc                                                        >  4:275291/1‑149 (MQ=255)
                                                                                            aaaGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggttc                                                        >  3:24201/1‑149 (MQ=255)
                                                                                            aaaGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggttc                                                        >  1:263338/1‑149 (MQ=255)
                                                                                               gACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggttccgg                                                     <  3:87262/149‑1 (MQ=255)
                                                                                                aCAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGAttt                                                                                                                             <  1:419178/76‑1 (MQ=255)
                                                                                                aCAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGAttt                                                                                                                             >  2:419178/1‑76 (MQ=255)
                                                                                                aCAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggttccggt                                                    >  3:481810/1‑149 (MQ=255)
                                                                                                aCAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGCCTACCTGCTGGTAGCCcgc                                                                                                                                  <  1:522499/71‑1 (MQ=37)
                                                                                                aCAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGCCTACCTGCTGGTAGCCcgc                                                                                                                                  >  2:522499/1‑71 (MQ=37)
                                                                                                  agagATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTa                                                  <  4:769090/149‑1 (MQ=255)
                                                                                                    agaTTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCAt                                                                                                 <  2:665498/100‑1 (MQ=255)
                                                                                                    agaTTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCAt                                                                                                 >  1:665498/1‑100 (MQ=255)
                                                                                                      aTTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACAcc                                              >  1:782991/1‑149 (MQ=255)
                                                                                                       ttGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCg                                             >  3:8505/1‑149 (MQ=255)
                                                                                                         gggTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGt                                           <  2:782991/149‑1 (MQ=255)
                                                                                                          ggtggtggtCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGtt                                          >  2:412745/1‑149 (MQ=255)
                                                                                                            tggtggtCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGc                                                                                                   <  4:472012/90‑1 (MQ=255)
                                                                                                             ggtggtCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTa                                       <  1:170444/149‑1 (MQ=255)
                                                                                                              gtggtCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTAc                                      <  1:278355/149‑1 (MQ=255)
                                                                                                              gtggtCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTAc                                      <  3:18672/149‑1 (MQ=255)
                                                                                                              gtggtCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTAc                                      <  1:363770/149‑1 (MQ=255)
                                                                                                               tggtCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTACt                                     <  4:116846/149‑1 (MQ=255)
                                                                                                                 gtCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTACtt                                    >  2:479402/1‑148 (MQ=255)
                                                                                                                   cGGCTGTACGCCACCGGAGATGCTCGACGCGGGCTACCATCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGTTTCCGGTTACACTGGTTTTACTTGCt                                 <  4:540693/149‑1 (MQ=255)
                                                                                                                    ggCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTACTTGCTa                                >  4:684334/1‑149 (MQ=255)
                                                                                                                       aGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTACTTGCTATGc                             <  4:707526/149‑1 (MQ=255)
                                                                                                                                   gagaTGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTACTTGCTATGCCGCACCGGATGt                 >  3:638188/1‑149 (MQ=255)
                                                                                                                                   gagaTGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTACTTGCTATGCCGCACCGGATGt                 <  4:646250/149‑1 (MQ=255)
                                                                                                                                     gaTGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggtt                                                         <  1:544562/107‑1 (MQ=255)
                                                                                                                                     gaTGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggtt                                                         >  2:544562/1‑107 (MQ=255)
                                                                                                                                        gCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTACTTGCTATGCCGCACCGGATGTCACGc            >  4:174501/1‑149 (MQ=255)
                                                                                                                                          tCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggttcc                                                       >  1:501811/1‑104 (MQ=255)
                                                                                                                                          tCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTACTTGCTATGCCGCACCGGATGTCACGCTg          >  1:509938/1‑149 (MQ=255)
                                                                                                                                          tCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTACTTGCTATGCCGCACCGGATGTCACGCTg          >  2:403488/1‑149 (MQ=255)
                                                                                                                                             tcgcgGCCTACCTGCTGGTAGCCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggttccggt                                                    <  4:435445/103‑1 (MQ=39)
                                                                                                                                             tcgcgGCCTACCTGCTGGTAGCCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggttccggt                                                    >  3:435445/2‑104 (MQ=39)
                                                                                                                                             tcgcgGCCTACCTGCTGGTAGCCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTACTTGCTATGCCGCACCGGATGTCACGCTGGaa       >  3:181239/2‑149 (MQ=255)
                                                                                                                                             aCGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTACTTGCTATGCCGCACCGGATGTCACGCTGGaa       <  2:774235/149‑1 (MQ=255)
                                                                                                                                             aCGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTACTTGCTATGCCGCACCGGATGTCACGCTGGaa       <  3:188111/149‑1 (MQ=255)
                                                                                                                                                  ggCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGATTTACTTGCTATGCCGCACCGGATGTCACGCTGGAAgatga  >  1:88751/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATTAGGAATTGTGTATTTGACGAAGGGATGATGGCGTGAAGATTTATCTGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTACACCGGTTTTACTTGCTATGCCGCACCGGATGTCACGCTGGAAGATGA  >  NC_000913/3201856‑3202150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: