Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,328,182 A→T 6.0% intergenic (+99/+57) yhbY → / ← greA RNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,328,1820AT 6.0% 282.6 / 3.2 83intergenic (+99/+57)yhbY/greARNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor
Reads supporting (aligned to +/- strand):  ref base A (33/44);  new base T (2/3);  total (35/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGC  >  NC_000913/3328035‑3328330
                                                                                                                                                   |                                                                                                                                                     
ggTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCAt                                                                                                                                                      <  1:188142/149‑1 (MQ=255)
  tGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAg                                                                                                                                                   >  1:777494/1‑149 (MQ=255)
         tCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCtt                                                                                                                                            <  1:496604/149‑1 (MQ=255)
           gCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCTATGCGGCCtttt                                                                                                                                          <  1:125028/149‑1 (MQ=255)
           gCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCtttt                                                                                                                                          >  1:263513/1‑149 (MQ=255)
             ccAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTc                                                                                                                                        >  4:754835/1‑149 (MQ=255)
             ccAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTc                                                                                                                                        >  4:319033/1‑149 (MQ=255)
             ccAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTc                                                                                                                                        >  1:50397/1‑149 (MQ=255)
             ccAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTc                                                                                                                                        <  1:412691/149‑1 (MQ=255)
              cAACTACAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCTATGCGGCCttttatc                                                                                                                                       <  1:433349/149‑4 (MQ=255)
              cAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTcc                                                                                                                                       <  4:860738/149‑1 (MQ=255)
              aaaCTAAAGAACGTAAAATCCCGCTGACACGCTAGGATTACCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCATAATGCCACTCTCTGTGCGTTGATAAAAGGCCGCTATGCGGCCttttatc                                                                                                                                       <  4:302582/148‑4 (MQ=255)
               aaCTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCTATGCGGCCttttatca                                                                                                                                      >  3:530154/1‑145 (MQ=255)
                 cTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCcttt                                                                                                                                    <  1:106606/149‑1 (MQ=255)
                 cTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCcttt                                                                                                                                    <  3:159407/149‑1 (MQ=255)
                 cTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCTATGCGGCCttttatcaac                                                                                                                                    <  4:530154/149‑7 (MQ=255)
                  tAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCctttc                                                                                                                                   <  3:801455/149‑1 (MQ=255)
                     aGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCtttt                                                                                                                                          <  3:771495/139‑1 (MQ=255)
                     aGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCtttt                                                                                                                                          >  4:771495/1‑139 (MQ=255)
                       aaCGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTAc                                                                                                                              >  3:39782/1‑149 (MQ=255)
                        aCGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTCTCTTTACa                                                                                                                             <  4:76120/149‑1 (MQ=255)
                                 ctcGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCaa                                                                                                                    <  3:391008/149‑1 (MQ=255)
                                     cTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATc                                                                                                                <  2:30343/149‑1 (MQ=255)
                                         cACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGa                                                                                                            <  1:345933/149‑1 (MQ=255)
                                         cACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGa                                                                                                            <  3:768610/149‑1 (MQ=255)
                                         cACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGa                                                                                                            <  4:120001/149‑1 (MQ=255)
                                         cACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGa                                                                                                            <  2:163623/149‑1 (MQ=255)
                                              tAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTAtt                                                                                                       >  3:263033/1‑149 (MQ=255)
                                               aaGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTg                                                                                                      <  3:780832/149‑1 (MQ=255)
                                                aGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTgg                                                                                                     <  1:587269/149‑1 (MQ=255)
                                                 gATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTggg                                                                                                    <  2:140440/149‑1 (MQ=255)
                                                    tATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTaa                                                                                                 >  4:654303/1‑149 (MQ=255)
                                                      tCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCGTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAAtt                                                                                               <  4:803531/149‑1 (MQ=255)
                                                        cgaaaGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCt                                                                                             >  1:205348/3‑149 (MQ=255)
                                                        cTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCt                                                                                             >  1:54742/1‑149 (MQ=255)
                                                        cTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCt                                                                                             <  1:549304/149‑1 (MQ=255)
                                                          aaaGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCtt                                                                                                              <  4:744028/130‑1 (MQ=255)
                                                          aaaGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCtt                                                                                                              >  3:744028/1‑130 (MQ=255)
                                                          aaaGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTa                                                                                           <  4:122055/149‑1 (MQ=255)
                                                          aaaGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTa                                                                                           <  2:763373/149‑1 (MQ=255)
                                                          aaaGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTa                                                                                           <  4:484501/149‑1 (MQ=255)
                                                              ttACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGCGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAgg                                                                                       >  3:361801/1‑149 (MQ=255)
                                                                  acacaTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTAtt                                                                                   >  4:775192/1‑149 (MQ=255)
                                                                    acaTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTcc                                                                                 >  1:471499/1‑149 (MQ=255)
                                                                     cacttGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTTCa                                                                                <  3:575948/146‑1 (MQ=255)
                                                                     caTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCa                                                                                >  4:35809/1‑149 (MQ=255)
                                                                          gCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTa                                                                           >  3:260126/1‑149 (MQ=255)
                                                                          gCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTa                                                                           >  1:283196/1‑149 (MQ=255)
                                                                            tgtgTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGTAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAAt                                                                         >  4:620383/1‑149 (MQ=255)
                                                                            tgtgTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCtt                                                                            >  2:838511/1‑146 (MQ=255)
                                                                            tgtgTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCtt                                                                            <  1:838511/146‑1 (MQ=255)
                                                                            tgtgTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAAt                                                                         <  1:458126/149‑1 (MQ=255)
                                                                               gtAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTAc                                                                      <  2:471499/149‑1 (MQ=255)
                                                                                  aaaCGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTaa                                                                                                 >  3:242178/1‑119 (MQ=255)
                                                                                  aaaCGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTaa                                                                                                 <  4:242178/119‑1 (MQ=255)
                                                                                  aaaCGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTc                                                                   >  4:342874/1‑149 (MQ=255)
                                                                                     cGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCaaa                                                                <  3:357900/149‑1 (MQ=255)
                                                                                            ttttCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACt                                                         >  3:500106/1‑149 (MQ=255)
                                                                                                    aggcaggAGAGCAAAATGCCACGCTTTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCgccgcc                                                 >  1:15026/1‑149 (MQ=255)
                                                                                                     ggcaggAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTGCGCCGccc                                                <  4:260126/149‑1 (MQ=255)
                                                                                                      gcaggAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCg                                               >  1:177986/1‑149 (MQ=255)
                                                                                                           agagCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGtt                                          <  2:14557/149‑1 (MQ=255)
                                                                                                            gagCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGttt                                         <  3:747334/149‑1 (MQ=255)
                                                                                                             agCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCaaa                                                                <  1:295154/125‑1 (MQ=255)
                                                                                                             agCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCaaa                                                                >  2:295154/1‑125 (MQ=255)
                                                                                                               cAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtga                                      <  4:263033/149‑1 (MQ=255)
                                                                                                               cAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtga                                      <  3:365576/149‑1 (MQ=255)
                                                                                                                aaaaTGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtgat                                     <  2:490950/149‑1 (MQ=255)
                                                                                                                aaaaTGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTAACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtgat                                     <  3:754835/149‑1 (MQ=255)
                                                                                                                 aaaTGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtgatg                                    >  4:338594/1‑149 (MQ=255)
                                                                                                                   aTGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGAc                                  <  4:157925/149‑1 (MQ=255)
                                                                                                                     gCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACca                                >  4:628467/1‑149 (MQ=255)
                                                                                                                      ccACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACcac                               <  1:835006/149‑1 (MQ=255)
                                                                                                                        aCGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCacaa                             <  3:628467/149‑1 (MQ=255)
                                                                                                                            tctGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAAcatc                         <  4:313498/149‑1 (MQ=255)
                                                                                                                                  cGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTCCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttc                   <  3:35809/149‑1 (MQ=255)
                                                                                                                                   gTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttct                  >  2:55383/1‑149 (MQ=255)
                                                                                                                                   gTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttct                  >  2:761873/1‑149 (MQ=255)
                                                                                                                                    ttGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttctt                 >  2:335625/1‑149 (MQ=255)
                                                                                                                                    ttGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttctt                 >  1:706938/1‑149 (MQ=255)
                                                                                                                                          aaaGGCCGCTATGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGTTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGACGTTTTGATGACCACAACATCATCTTCTTCTTTGc           >  3:518043/1‑149 (MQ=255)
                                                                                                                                           aaGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGcc          <  2:777494/149‑1 (MQ=255)
                                                                                                                                                 gCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCTTTTTGATGCCCACAACATCATCTTCTTCTTTGCCGATCAg    >  4:623905/1‑149 (MQ=255)
                                                                                                                                                   aTAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGc  <  2:263513/149‑1 (MQ=255)
                                                                                                                                                   aTAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGc  <  4:39782/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGC  >  NC_000913/3328035‑3328330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: