Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,426,592 T→A 6.0% intergenic (+35/+36) rplI → / ← yjfZ 50S ribosomal subunit protein L9/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,426,5920TA 6.0% 341.0 / 9.7 99intergenic (+35/+36)rplI/yjfZ50S ribosomal subunit protein L9/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (46/47);  new base A (4/2);  total (50/49)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

Only 100 of 102 total aligned reads displayed.

AGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCACTCGCT  >  NC_000913/4426444‑4426740
                                                                                                                                                    |                                                                                                                                                    
agCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCAt                                                                                                                                                      >  4:245261/1‑149 (MQ=255)
agCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTAACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCAt                                                                                                                                                      >  2:438134/1‑149 (MQ=255)
  cGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTg                                                                                                                                                    >  1:534838/1‑149 (MQ=255)
      gTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTAACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCg                                                                                                                                                <  1:438134/149‑1 (MQ=255)
           tCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGtt                                                                                                                                           <  4:141185/149‑1 (MQ=255)
                cGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCt                                                                                                                                      >  3:805006/1‑149 (MQ=255)
                 gAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCtt                                                                                                                                     <  3:466489/149‑1 (MQ=255)
                        gTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGTTCGTAAACGTAGTAGCTGAATAATTCGTTATGCAACGAGACATAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTAttt                                                                                                                              >  3:726977/1‑149 (MQ=255)
                         ttCTGCGTACCACTGGCGAACACTAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCAACGATTGTTCGGCTTTTTGTTTTCTGtttt                                                                                                                             >  4:459262/1‑149 (MQ=255)
                                tACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAg                                                                                                                      <  3:392383/149‑1 (MQ=255)
                                    aCTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAg                                                                                                                      >  2:467234/1‑145 (MQ=255)
                                    aCTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAg                                                                                                                      <  1:467234/145‑1 (MQ=255)
                                    aCTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTAt                                                                                                                  >  3:299920/1‑149 (MQ=255)
                                        gCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGt                                                                                                              <  4:805006/149‑1 (MQ=255)
                                          gAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGttt                                                                                                            >  1:258420/1‑149 (MQ=255)
                                          gAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGttt                                                                                                            >  1:196438/1‑149 (MQ=255)
                                             cacGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGc                                                                                                         <  3:125207/149‑1 (MQ=255)
                                              acGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCa                                                                                                        >  2:166992/1‑149 (MQ=255)
                                                gAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAg                                                                                                      >  3:464075/1‑149 (MQ=255)
                                                     gAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGAt                                                                                                 >  4:668317/1‑149 (MQ=255)
                                                         ttCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCAt                                                                                             <  4:293116/149‑1 (MQ=255)
                                                          tCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCAtt                                                                                            >  1:857317/1‑149 (MQ=255)
                                                           ccAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTc                                                                                           <  3:10970/149‑1 (MQ=255)
                                                             aGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCAc                                                                                         >  2:636782/1‑149 (MQ=255)
                                                                   acaGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATtct                                                                                   >  2:124315/1‑149 (MQ=255)
                                                                      gCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCtt                                                                                <  3:237098/149‑1 (MQ=255)
                                                                      gCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCtt                                                                                <  1:741349/149‑1 (MQ=255)
                                                                        gAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTtgt                                                                              <  2:700375/149‑1 (MQ=255)
                                                                            tATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGtttt                                                                                                                                         <  1:674904/86‑1 (MQ=255)
                                                                            tATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGtttt                                                                                                                                         >  2:674904/1‑86 (MQ=255)
                                                                            tATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTcc                                                                          >  1:857003/1‑149 (MQ=255)
                                                                             aTTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCa                                                                         >  3:616791/1‑149 (MQ=255)
                                                                                 gcgAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCt                                                                                                                                      <  2:557714/84‑1 (MQ=255)
                                                                                 gcgAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCt                                                                                                                                      >  1:557714/1‑84 (MQ=255)
                                                                                 gcgAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAg                                                                     >  4:791375/1‑149 (MQ=255)
                                                                                 gcgAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAg                                                                     >  2:763550/1‑149 (MQ=255)
                                                                                   gAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTc                                                                   <  3:791375/149‑1 (MQ=255)
                                                                                    aaaGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCa                                                                  <  3:668317/149‑1 (MQ=255)
                                                                                    aaaGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCa                                                                  <  2:251661/149‑1 (MQ=255)
                                                                                     aaGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAg                                                                 <  1:698168/149‑1 (MQ=255)
                                                                                         gATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTg                                                                                                                                                    >  2:271233/1‑62 (MQ=255)
                                                                                         gATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTg                                                                                                                                                    <  1:271233/62‑1 (MQ=255)
                                                                                         gATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATg                                                             >  4:317425/1‑149 (MQ=255)
                                                                                          aTCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATgg                                                            >  2:417307/1‑149 (MQ=255)
                                                                                          aTCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATgg                                                            >  4:695568/1‑149 (MQ=255)
                                                                                          aTCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATgg                                                            >  2:130334/1‑149 (MQ=255)
                                                                                           tCGTAAACGTAGTAGCTAAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCCGGATGGt                                                           <  3:459262/149‑1 (MQ=255)
                                                                                               aaaCGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGc                                                       >  3:28652/1‑149 (MQ=255)
                                                                                                aaCGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCt                                                      >  4:616787/1‑149 (MQ=255)
                                                                                                aaCGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCt                                                      <  1:430004/149‑1 (MQ=255)
                                                                                                 aCGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTg                                                     <  2:494361/149‑1 (MQ=255)
                                                                                                 aCGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTg                                                     <  4:680366/149‑1 (MQ=255)
                                                                                                     agtagCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGt                                                 <  1:7112/149‑1 (MQ=255)
                                                                                                       tagCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTa                                               <  3:719299/149‑1 (MQ=255)
                                                                                                         gCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTc                                                                                                           >  2:246517/1‑87 (MQ=255)
                                                                                                         gCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTc                                                                                                           <  1:246517/87‑1 (MQ=255)
                                                                                                         gCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGa                                             <  4:722119/149‑1 (MQ=255)
                                                                                                           tGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGa                                             <  1:531761/147‑1 (MQ=255)
                                                                                                           tGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCAGTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGa                                             >  2:531761/1‑147 (MQ=255)
                                                                                                            gAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGatac                                          >  1:279524/1‑149 (MQ=255)
                                                                                                            gAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGatac                                          >  3:162792/1‑149 (MQ=255)
                                                                                                             aataatTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGataca                                         <  1:124315/149‑1 (MQ=255)
                                                                                                                aatTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGatacatac                                      <  1:415132/149‑1 (MQ=255)
                                                                                                                aacgaattATTCAGCGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGatacatac                                      >  1:285168/7‑149 (MQ=255)
                                                                                                                  ttCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGa                                    <  3:214673/149‑1 (MQ=255)
                                                                                                                  ttCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGa                                    >  4:787733/1‑149 (MQ=255)
                                                                                                                  ttCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGa                                    <  3:787733/149‑1 (MQ=255)
                                                                                                                  ttCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGa                                    <  4:312941/149‑1 (MQ=255)
                                                                                                                  ttCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGa                                    >  1:328082/1‑149 (MQ=255)
                                                                                                                  ttCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGa                                    >  4:214673/1‑149 (MQ=255)
                                                                                                                    cGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAgc                                  <  3:245261/149‑1 (MQ=255)
                                                                                                                    cGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAgc                                  <  4:763121/149‑1 (MQ=255)
                                                                                                                     gTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAgcg                                 >  1:395919/1‑149 (MQ=255)
                                                                                                                     gTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAgcg                                 >  3:116587/1‑149 (MQ=255)
                                                                                                                        aTTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCaa                              <  2:279524/149‑1 (MQ=255)
                                                                                                                        aTTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCaa                              <  1:763550/149‑1 (MQ=255)
                                                                                                                         ttCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAAc                             <  1:3563/149‑1 (MQ=255)
                                                                                                                            aaCGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGa                          <  3:3253/149‑1 (MQ=255)
                                                                                                                            aaCGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGa                          >  3:675400/1‑149 (MQ=255)
                                                                                                                             aCGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGAt                         <  1:379071/149‑1 (MQ=255)
                                                                                                                             aCGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGAt                         <  3:151542/149‑1 (MQ=255)
                                                                                                                                  aCGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGtctc                    <  2:134337/149‑1 (MQ=255)
                                                                                                                                    gTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCa                                                                         <  1:470269/94‑1 (MQ=255)
                                                                                                                                    gTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCa                                                                         >  2:470269/1‑94 (MQ=255)
                                                                                                                                    gTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGc                  >  3:83126/1‑149 (MQ=255)
                                                                                                                                    gTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGc                  <  4:464075/149‑1 (MQ=255)
                                                                                                                                       aaaaCGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTcc               <  1:417307/149‑1 (MQ=255)
                                                                                                                                        aaaCGCCGACCAATGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCt              >  2:686594/1‑149 (MQ=255)
                                                                                                                                        aaaCGCCGACCAATGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCt              >  1:298691/1‑149 (MQ=255)
                                                                                                                                          aCGCCGACCAATGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTtgt                                                                              <  2:72567/83‑1 (MQ=255)
                                                                                                                                          aCGCCGACCAATGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTtgt                                                                              >  1:72567/1‑83 (MQ=255)
                                                                                                                                          aCGCCGACCAATGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGa                          >  2:190293/1‑135 (MQ=255)
                                                                                                                                          aCGCCGACCAATGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGa                          <  1:190293/135‑1 (MQ=255)
                                                                                                                                               gACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCAc       >  2:12265/1‑149 (MQ=255)
                                                                                                                                               gACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAAATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCAc       >  3:569518/1‑149 (MQ=255)
                                                                                                                                                  cATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCActcg    >  2:551884/1‑149 (MQ=255)
                                                                                                                                                  cATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCActcg    >  3:187946/1‑149 (MQ=255)
                                                                                                                                                   aTTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCActcgc   <  4:436249/149‑1 (MQ=255)
                                                                                                                                                   aTTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCActcgc   <  2:196438/149‑1 (MQ=255)
                                                                                                                                                   aTTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCActcgc   >  1:583023/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGCGAAGTTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGCGGCACTCGCT  >  NC_000913/4426444‑4426740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: