Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,202,957 G→T 6.2% intergenic (‑24/‑67) cohE ← / → croE e14 prophage; repressor protein phage e14/e14 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,202,9570GT 6.2% 456.8 / 15.6 130intergenic (‑24/‑67)cohE/croEe14 prophage; repressor protein phage e14/e14 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (51/70);  new base T (3/5);  total (55/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

Only 100 of 132 total aligned reads displayed.

TCTCCCATAAACCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCAACACCTGGCTACTTAC  >  NC_000913/1202809‑1203105
                                                                                                                                                    |                                                                                                                                                     
tctcCCATAAACCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCg                                                                                                                                                       >  2:506123/1‑149 (MQ=255)
    ccATAAACCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTCaa                                                                                                                                                   >  4:837490/1‑149 (MQ=255)
     cATAAACCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAg                                                                                                                                                 >  3:79166/1‑149 (MQ=255)
      aTAAACCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAgg                                                                                                                                                >  1:719159/1‑149 (MQ=255)
      aTAAACCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAgg                                                                                                                                                >  1:769302/1‑149 (MQ=255)
        aaaCCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTa                                                                                                                                              <  1:367744/149‑1 (MQ=255)
         aaCCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTAc                                                                                                                                             >  4:803364/1‑149 (MQ=255)
         aaCCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTAc                                                                                                                                             <  1:576786/149‑1 (MQ=255)
           ccTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACaa                                                                                                                                           <  2:463529/149‑1 (MQ=255)
           ccTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACaa                                                                                                                                           <  3:687259/149‑1 (MQ=255)
            ctgctgGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAg                                                                                                                                          >  4:372371/1‑149 (MQ=255)
             tgctgGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCTTAACGGTACAAgg                                                                                                                                         <  1:116989/149‑2 (MQ=255)
              gctgGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCTGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCTTAACGGTACAAggg                                                                                                                                        <  1:358007/149‑3 (MQ=255)
               ctgGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACACTTGTACCTTAACGGTACAAgggt                                                                                                                                       <  2:417380/149‑4 (MQ=255)
                 ggTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCt                                                                                                                                     <  3:756397/149‑1 (MQ=255)
                  gTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGGCAACATCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCtt                                                                                                                                    <  3:713760/149‑1 (MQ=255)
                     tGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGaa                                                                                                                                 <  1:846640/149‑1 (MQ=255)
                      gggTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAg                                                                                                                                <  2:203689/149‑1 (MQ=255)
                      gggTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAg                                                                                                                                <  4:290706/149‑1 (MQ=255)
                      gggTTGGCTCGCGACCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAg                                                                                                                                <  4:824321/149‑1 (MQ=255)
                        gTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGt                                                                                                                              >  1:20009/1‑149 (MQ=255)
                         ttGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGtt                                                                                                                             >  4:777341/1‑149 (MQ=255)
                         ttGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGtt                                                                                                                             >  2:270237/1‑149 (MQ=255)
                                 cgATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTTAAGGTTCATTTCaa                                                                                                                     <  4:79166/149‑1 (MQ=255)
                                 cgATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCaa                                                                                                                     <  2:528113/149‑1 (MQ=255)
                                 cgATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCaa                                                                                                                     <  1:432128/149‑1 (MQ=255)
                                    tcaatcaTAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCa                                                                                                                  >  1:271662/1‑149 (MQ=255)
                                    tcaatcaTAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCa                                                                                                                  <  4:25080/149‑1 (MQ=255)
                                     caatcaTAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCAt                                                                                                                 <  4:383620/149‑1 (MQ=255)
                                     caatcaTAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCAt                                                                                                                 <  4:297879/149‑1 (MQ=255)
                                      aatcaTAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATg                                                                                                                >  4:441049/1‑149 (MQ=255)
                                       atcaTAGTGGCAAAGGAAGAATTCCCGCCAACACCATGTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAATGTACAAGTATCTTGAAGGTTCATTTCAATCATGt                                                                                                               >  1:720138/1‑149 (MQ=255)
                                        tcaTAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTa                                                                                                              >  4:455170/1‑149 (MQ=255)
                                         caTAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTaa                                                                                                             >  1:535376/1‑149 (MQ=255)
                                               ggCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAatat                                                                                                          >  2:178945/1‑146 (MQ=255)
                                               ggCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAatat                                                                                                          <  1:178945/146‑1 (MQ=255)
                                               ggCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTa                                                                                                       <  2:720138/149‑1 (MQ=255)
                                                  aaaGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTacac                                                                                                    >  1:839116/1‑149 (MQ=255)
                                                  aaaGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTacac                                                                                                    >  2:384276/1‑149 (MQ=255)
                                                       aagaaTTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAg                                                                                               <  3:667664/149‑1 (MQ=255)
                                                         gaaTTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGt                                                                                             <  4:145267/149‑1 (MQ=255)
                                                            ttCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACa                                                                                          >  2:476867/1‑149 (MQ=255)
                                                             tCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACat                                                                                         <  1:506123/149‑1 (MQ=255)
                                                                cGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATAtt                                                                                      <  2:579712/149‑1 (MQ=255)
                                                                cGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATAtt                                                                                      <  1:152960/149‑1 (MQ=255)
                                                                   cAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTa                                                                                   <  1:783677/149‑1 (MQ=255)
                                                                     acacCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTaa                                                                                                                                                   >  2:309158/1‑84 (MQ=255)
                                                                     acacCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTaa                                                                                                                                                   <  1:309158/84‑1 (MQ=255)
                                                                       acCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGaa                                                                               >  1:784104/1‑149 (MQ=255)
                                                                       acCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGCTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGaa                                                                               >  4:581784/1‑149 (MQ=255)
                                                                        ccATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGaaa                                                                              <  3:615672/149‑1 (MQ=255)
                                                                        ccATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGaaa                                                                              <  3:634279/149‑1 (MQ=255)
                                                                         cATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATAtt                                                                                      <  2:747830/140‑1 (MQ=255)
                                                                          agctctcAGTTTTCTGGGGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTATAACCTTTGTACCGTTAAGGGCCAAGTATCTTGAAGGTTAATTTCAGTCATGTAATATGTAAACCGGAGGTACATATTGTATGAAAGc                                                                            >  4:695523/3‑149 (MQ=255)
                                                                             tctcAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTa                                                                         >  4:132106/1‑149 (MQ=255)
                                                                              ctcAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTAt                                                                        <  4:108229/149‑1 (MQ=255)
                                                                               tcAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCTTAACGGTACAAggg                                                                                                                                        <  3:845192/84‑3 (MQ=38)
                                                                               tcAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTAtt                                                                       >  2:826369/1‑149 (MQ=255)
                                                                               tcAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTAtt                                                                       >  3:304594/1‑149 (MQ=255)
                                                                                cAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTg                                                                      <  2:271662/149‑1 (MQ=255)
                                                                                 aGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTgg                                                                     <  1:541527/149‑1 (MQ=255)
                                                                                 aGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTgg                                                                     <  4:368639/149‑1 (MQ=255)
                                                                                           cGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTaa                                                           <  2:719159/149‑1 (MQ=255)
                                                                                           cGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTaa                                                           <  2:769302/149‑1 (MQ=255)
                                                                                           cGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTaa                                                           <  1:826369/149‑1 (MQ=255)
                                                                                            gTTAGACCGCCGGTTGTCATGGATTGTTTTCATAACGAAATTACAACCCTTGTACCGTTAATGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCttt                                                             <  4:703746/146‑1 (MQ=255)
                                                                                            gTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTATCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAAc                                                          >  1:567380/1‑149 (MQ=255)
                                                                                            gTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCttt                                                             >  3:703746/1‑146 (MQ=255)
                                                                                            gTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAAc                                                          >  3:40117/1‑149 (MQ=255)
                                                                                            gTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAAc                                                          >  1:185525/1‑149 (MQ=255)
                                                                                             ttAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAGTCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAAcc                                                         >  1:768876/1‑149 (MQ=255)
                                                                                              tAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCa                                                        <  2:704527/149‑1 (MQ=255)
                                                                                                gACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCaaa                                                      <  3:265722/149‑1 (MQ=255)
                                                                                                gACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCaaa                                                      <  3:777341/149‑1 (MQ=255)
                                                                                                         aTGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAAcagcag                                             <  4:181299/149‑1 (MQ=255)
                                                                                                          tGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAgg                                            <  3:620340/149‑1 (MQ=255)
                                                                                                           gTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAggg                                           <  1:430079/149‑1 (MQ=255)
                                                                                                           gTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAggg                                           <  1:736230/149‑1 (MQ=255)
                                                                                                              aTGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAAc                                                          >  2:875261/1‑131 (MQ=255)
                                                                                                              aTGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAAc                                                          <  1:875261/131‑1 (MQ=255)
                                                                                                              aTGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGa                                        >  2:118581/1‑149 (MQ=255)
                                                                                                               tGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAg                                       <  2:458552/149‑1 (MQ=255)
                                                                                                                  aTTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTg                                    >  1:596948/1‑149 (MQ=255)
                                                                                                                   ttGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTgg                                   >  1:174357/1‑149 (MQ=255)
                                                                                                                     gTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGcc                                 <  1:82463/149‑1 (MQ=255)
                                                                                                                      ttttCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCg                                >  3:390427/1‑149 (MQ=255)
                                                                                                                           aTAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGaaaa                           <  4:304594/149‑1 (MQ=255)
                                                                                                                            tAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAGTCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAg                          <  2:768876/149‑1 (MQ=255)
                                                                                                                             aaCGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGt                         >  4:754656/1‑149 (MQ=255)
                                                                                                                                gAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTgg                      <  3:441049/149‑1 (MQ=255)
                                                                                                                                 aaaTTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGc                     <  4:632837/149‑1 (MQ=255)
                                                                                                                                    ttAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCa                  >  2:236041/1‑149 (MQ=255)
                                                                                                                                         aCCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCAACAcc             >  3:161047/1‑149 (MQ=255)
                                                                                                                                             ttGTACCTTAACGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGaaaa                           >  1:432325/1‑131 (MQ=255)
                                                                                                                                             ttGTACCTTAACGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGaaaa                           <  2:432325/131‑1 (MQ=255)
                                                                                                                                             ttGTACCTTAACGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCAACACCTGGc         >  1:265542/1‑149 (MQ=255)
                                                                                                                                             ttGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCAACAcc             <  1:375427/145‑1 (MQ=255)
                                                                                                                                             ttGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCAACAcc             >  2:375427/1‑145 (MQ=255)
                                                                                                                                             ttGTACCTTAACGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCAACACCTGGc         >  3:345988/1‑149 (MQ=255)
                                                                                                                                                  ccGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCAACACCTGGCTACtt    <  1:118581/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCTCCCATAAACCTGCTGGTCTGGGTTGGCTCGCGATCAATCATAGTGGCAAAGGAAGAATTCCCGCCAACACCATCTCTCAGTTTTCTGGCGTTAGACCGCCGGATGTCATGGATTGTTTTCATAACGAAATTAAAACCCTTGTACCGTTAAGGTACAAGTATCTTGAAGGTTCATTTCAATCATGTAATATGTACACCGGAGGTACATATTGTATGAAAGCGTATTGGGACTCTTTAACCAAAGAACAGCAGGGCGAGTTGGCCGGAAAAGTTGGCTCAACACCTGGCTACTTAC  >  NC_000913/1202809‑1203105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: