Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,536,246 C→A P138Q (CCG→CAG)  crr → glucose‑specific enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,536,2460CA98.2% 364.3 / NA 113P138Q (CCG→CAG) crrglucose‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (50/61);  total (51/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

TGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCAC  >  NC_000913/2535974‑2536516
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                               
tGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAATTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAgg                                                                                                                                                                                                                                                                               >  1:59673/1‑275 (MQ=255)
   tgattggaattatATCAAACCAAAAGGTAACAAAATTGTCGCACCAGTAGACGCCACCATTGGTAAAAGCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATATCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGgttgt                                                                                                                                                                                                                                                                           <  4:54221/264‑1 (MQ=255)
           ttGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCCTTGGTAACATCTTTGAAACCAACCACGCATTCACAATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAGAGGCGAAGGCTTCAAGCGTATTGCTGAATCAGGTCAGCGCGTGAAAGTTGGCGAGACTGTCATTGAATTTGATCTGCCGCTGCGTGCAGGGCAGGCCAAGGCAACCCTGCCCCAGGTTGTTTTCGCCa                                                                                                                                                                                                                                                                  >  4:202253/1‑276 (MQ=255)
            tGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCa                                                                                                                                                                                                                                                                  >  3:453362/1‑275 (MQ=255)
             gCTATCAAACCAACGGGTAACAAAATGGTCGCGTCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGTTAGCGGCGTTGCACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTCCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACCCAGGTTGTTATCTCCaa                                                                                                                                                                                                                                                                 >  3:322312/1‑275 (MQ=255)
                    aaCCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGAc                                                                                                                                                                                                                                                          <  1:468888/275‑1 (MQ=255)
                     aCCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTAtct                                                                                                                                                                                                                                                                     <  2:368656/263‑1 (MQ=255)
                      ccAACGGGTAACAAAATGGTCCCGCCAGTATACGGCACCATTGTTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGa                                                                                                                                                                                                                                                        <  3:263326/275‑1 (MQ=255)
                      ccAACGGGTAACAAAATGGGCTAGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCAAGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGa                                                                                                                                                                                                                                                        <  2:589632/275‑1 (MQ=255)
                         aCGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAAt                                                                                                                                                                                                                                                     >  1:347693/1‑275 (MQ=255)
                           gggTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCACGTCTACCCTGACACAGGTTGTTCTCTCTAACAGGGAGGAAATaaa                                                                                                                                                                                                                                                  >  4:501010/1‑276 (MQ=255)
                            ggTAACAAACTGGCTGCGCCAGTAGACGGCACCATTTGTAAACTCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGTCGTTNAACTGTTCGTCCCCTTCGGTATCGACACCGTTGAACTGCAAGGCGAAGCCTTCAAGCGTATTGCTGACGACGGTCAGCCCGTGAAAGTTGAGGATCCTGTCTTTTAATTTGATCTGACGTTGCTGGAACAGAAACCCCATTCTACCCTTCTTCCGTTAGTTATCCCCAACCCGGCCGAACTCaa                                                                                                                                                                                                                                                  >  4:329862/1‑275 (MQ=255)
                             gTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATc                                                                                                                                                                                                                                                    >  1:514088/1‑272 (MQ=255)
                              tAACAAAATGGGCGCGCCAGTAGACGGCACCATTGGTAAAATCATTGAAACCAACCACGCATTCTCTATCGAATCTGAGAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGa                                                                                                                                                                                                                                               <  2:173346/276‑1 (MQ=255)
                                aCAAAATGTTCGCGCCAATAGACGGCACCATTGGTAAAATCTTTGAAGCCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCGAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGaa                                                                                                                                                                                                                                              <  1:569503/275‑1 (MQ=255)
                                  aaaaTGAGCGGGCCAGTAGACGGCACCATTGGTAAAATTCTTGAAACCTACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTg                                                                                                                                                                                                                                           <  4:287221/276‑1 (MQ=255)
                                       ggTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCaa                                                                                                                                                                                                                                      >  2:376486/1‑276 (MQ=255)
                                       ggTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTGGTTATCTCCAACATGGAGGAAATCAAATAACTGATCa                                                                                                                                                                                                                                       >  3:50658/1‑275 (MQ=255)
                                        gTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCaaa                                                                                                                                                                                                                                     <  2:489064/276‑1 (MQ=255)
                                               cAGGAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATTCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTcc                                                                                                                                                                                                                               <  2:49055/276‑1 (MQ=255)
                                                 gTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCgg                                                                                                                                                                                                                             <  3:347693/275‑1 (MQ=255)
                                                 gTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCgg                                                                                                                                                                                                                             <  1:377501/275‑1 (MQ=255)
                                                  tAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAAATGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATTTCCAACATGGACGAAATCAAAGAACTCAACAAACAGTCCGGt                                                                                                                                                                                                                            >  3:337599/1‑275 (MQ=255)
                                                       ggCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTCGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATACAAGAACTGATCAAACTGTCCGGTAGCGt                                                                                                                                                                                                                       >  3:487313/1‑275 (MQ=255)
                                                         cACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTa                                                                                                                                                                                                                      <  1:101881/274‑1 (MQ=255)
                                                         cACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACAGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAATAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGATAAAGCCAAGTCTACACTGACTCAGGTTGTTATCTGTAACATGGACGAAATCAAAGACCGGATCAAACTGGCAGGttggttaa                                                                                                                                                                                                                     >  4:284103/1‑268 (MQ=255)
                                                         cACCATTGGGAAAATCCTTGAAACCAGCCTCGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTa                                                                                                                                                                                                                      <  1:528810/274‑1 (MQ=255)
                                                            cATTGGTAAAATCATTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCGGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCg                                                                                                                                                                                                                  <  2:151903/275‑1 (MQ=255)
                                                              ttGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGACGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACAGATCAAACTGTCCGGTAGCGTAAACGTgg                                                                                                                                                                                                               >  2:71904/1‑276 (MQ=255)
                                                              ttGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGGATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAATTATACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCAGGTAGCGTAACCGTg                                                                                                                                                                                                                >  1:524669/1‑275 (MQ=255)
                                                                 gTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGt                                                                                                                                                                                                             <  3:514088/275‑1 (MQ=255)
                                                                 gTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGt                                                                                                                                                                                                             <  1:453362/275‑1 (MQ=255)
                                                                   aaacacTTTGAAACCAACCACGCATTCTCTTGCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTAACGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTg                                                                                                                                                                                                            <  4:218312/269‑1 (MQ=255)
                                                                           tGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGt                                                                                                                                                                                                  >  2:27827/1‑276 (MQ=255)
                                                                                      aCGCATTCCCATTGGAATCGGATAGCGGCGTTGAACTTTTCGTCCACTTCGGTATCGAAACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCa                                                                                                                                                                                       <  4:53867/276‑1 (MQ=255)
                                                                                              tctATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAATTCTACCCTGACTCAGGTTGTTATCTCCAAAATGGAAGACCTCAAAGACCTGATCAAACTGTCCGCGAACTTAACCGTTGGGAAAGCACCGGTTACCCGCATCAAGAAGTaa                                                                                                                                                                               >  4:456091/1‑276 (MQ=255)
                                                                                               ctATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTaa                                                                                                                                                                               >  3:262785/1‑275 (MQ=255)
                                                                                                  tCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCTTTACTCAGGTTGTTATCCTCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGCAACCCCGGTTATCTGCATCAAGGAGGAAtac                                                                                                                                                                            >  3:192734/1‑273 (MQ=255)
                                                                                                     aagcTGGTAGAGGCGTTTAACTGTTCGTCCACCTCGTTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGc                                                                                                                                                                        <  2:549822/273‑1 (MQ=255)
                                                                                                        cTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGCAGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCACGGTTATCCGCATCAAGAAGTAATTCTTGcag                                                                                                                                                                      >  2:270747/1‑273 (MQ=255)
                                                                                                        cTGATAACGGCGTGGAACTGTTCGTCCACTTAGGTATCGACAACGGTGAACTGAAAGGCGAAGTCTTCAAGCGTATTGCTGAAGGAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTAACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCg                                                                                                                                                                      <  3:337329/275‑1 (MQ=255)
                                                                                                          gATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTgg                                                                                                                                                                                                               >  3:18078/1‑232 (MQ=255)
                                                                                                              gcggcgTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGa                                                                                                                                                                                                           <  1:18078/232‑1 (MQ=255)
                                                                                                                 gcgTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAAATATTGCCGCAGTGaaa                                                                                                                                                              >  1:492167/1‑274 (MQ=255)
                                                                                                                             tCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCAt                                                                                                                                                  <  1:262785/274‑1 (MQ=255)
                                                                                                                                    cTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCa                                                                                                                                          >  2:419897/1‑275 (MQ=255)
                                                                                                                                     ttCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGgtt                                                                                                                                                                                                                                                                             <  1:195442/144‑1 (MQ=255)
                                                                                                                                      tCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCAt                                                                                                                                         >  1:605619/1‑274 (MQ=255)
                                                                                                                                          tATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCATGGGTGAAACCCCGGTTATCCGCATCAAGCAGTAATTCTTGCGGCAGTGAAAAATGGCGCCCATCGGCGCCAtttttt                                                                                                                                    >  2:203932/1‑275 (MQ=255)
                                                                                                                                                   cGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTcc                                                                                                                           >  3:238696/1‑275 (MQ=255)
                                                                                                                                                   cGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCAATCGGCGCCATTTTTTTATGCTTCCg                                                                                                                          >  4:352884/1‑276 (MQ=255)
                                                                                                                                                    gTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCCTTTTTTTATGCTTCCg                                                                                                                          >  3:412508/1‑275 (MQ=255)
                                                                                                                                                       gAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGa                                                                                                                                                                                                                                               <  3:564014/155‑1 (MQ=255)
                                                                                                                                                          cTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATTCTTCCGACAgcgg                                                                                                                   >  2:271364/1‑276 (MQ=255)
                                                                                                                                                               aGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTGTGCTTCCGCCAGCGGCGGCa                                                                                                              >  2:138051/1‑276 (MQ=255)
                                                                                                                                                               aGGCGAAGGCTTCAAGCGGATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCggcggc                                                                                                               <  1:266823/275‑1 (MQ=255)
                                                                                                                                                                           cAATCGTATGGCTGAAGCAGGTCATCGCGTGAATGTGGGCGATACTGGCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTTAATCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCAt                                                                                                  <  2:202253/276‑1 (MQ=255)
                                                                                                                                                                              gggaaTTTCTGAGGAATGTCAGCGCGTGAAAGTTGGCGATACTGTCATTTAATTTGATCTGCCGCTGCTTGAAGAGAAAGCCAAGTCTACCCTTACTCAGGTTGTTATCGCCAAAATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTGTGCTTCCGCCAGCGGCGGCAAAATCAATTCATCg                                                                                                <  4:138051/271‑1 (MQ=255)
                                                                                                                                                                               cGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTTCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGc                                                                                               <  4:167794/275‑1 (MQ=255)
                                                                                                                                                                                   ttGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTCTCTCCAACATGGACGACATCACAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCGCCTCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGAAAAATCAATTCATCGATCTCa                                                                                          >  2:320401/1‑276 (MQ=255)
                                                                                                                                                                                      ttagaaaagGTCAGCGCAGGAAATTGCGAGATACTGTCATTGAATTGGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTAGACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCAtg                                                                                        <  4:300241/269‑1 (MQ=255)
                                                                                                                                                                                      cgtaagaagGTCGGGGCGTGAAAATTGGGGATAAGGTAGTTGAATTTGGGCGGGTGCTGATGGAAGAGAAAGCCAAATATGACACGAACAAGGTTGTTATCACCAACATGGCCGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGAATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCAtg                                                                                        <  4:286968/272‑1 (MQ=255)
                                                                                                                                                                                      cTGAAGAAGGTCAGCGCGGGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCa                                                                                          <  1:198124/273‑1 (MQ=255)
                                                                                                                                                                                      cTGAAGAAGATCGGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCAtg                                                                                        <  2:457794/275‑1 (MQ=255)
                                                                                                                                                                                      atGAAGAAGGTAAGCGCGTGAAAGTTGGCGATAATGGCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCAtg                                                                                        <  4:419897/274‑1 (MQ=255)
                                                                                                                                                                                       tGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCAtg                                                                                        <  1:412508/274‑1 (MQ=255)
                                                                                                                                                                                       tGAAGAAGGTCAGCGCGTAAAAGTTGCCTATACTGTCAGTGAAATTGACCTGCCACTGCGGAAAGAGGAAGACAAGTCTACCCTGACTCAGGTTGTTAGCTCCAACATAGACGAAAACAAAGAAATGATCAAACAGTCCGGTAGCGTAACCGAGGATGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAGATCAATTCATCGCTCTCAtg                                                                                        <  3:392553/274‑1 (MQ=255)
                                                                                                                                                                                         aagaagGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCAtgc                                                                                       >  1:457652/1‑273 (MQ=255)
                                                                                                                                                                                          ggaaaGTCAGCGAGAGAAATGTGGCGAGACTTTCATTGAATTTTAACTGCCTGTGCTGGAAGAGAAAGCCAAGTCTACCCCGACTCAGGTTTTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATNCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATgctgct                                                                                   <  4:326993/275‑1 (MQ=255)
                                                                                                                                                                                          gaattgtcggtgcgTGAAAGTTGGAGGTACTGTCATGTAATTTTATATGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTTTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATgctgct                                                                                   <  4:546509/265‑1 (MQ=255)
                                                                                                                                                                                          agaagGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATgctgct                                                                                   <  2:388062/276‑1 (MQ=255)
                                                                                                                                                                                           gaagGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATgctgct                                                                                   <  1:238696/275‑1 (MQ=255)
                                                                                                                                                                                           gaagGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGAAGTGAAACATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATgctcct                                                                                   >  3:152145/1‑275 (MQ=255)
                                                                                                                                                                                                tCAGCGGGTGAAAGTTGGCAGTACTGTCATTGAATTTTATCTAGCGGTGCTGGAAGAGAAAACCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGGCGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGgtg                                                                              <  3:524669/275‑1 (MQ=255)
                                                                                                                                                                                                   gcgcGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCCGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCAGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAg                                                                           >  3:213926/1‑275 (MQ=255)
                                                                                                                                                                                                    cgcgTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTGTCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATc                                                                                                 >  1:205165/1‑252 (MQ=255)
                                                                                                                                                                                                       gTGAAAGTTGGAGATACTGTCATCGAATTTGATCAGCCGCTGCTGGAAGAGAAAGCAAAGTCTACCCTGACTCAGGTTGTTATCTCCAACAGGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCAt                                                                      <  4:168323/276‑1 (MQ=255)
                                                                                                                                                                                                        tGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGctc                                                                                             <  3:205165/252‑1 (MQ=255)
                                                                                                                                                                                                                  cGATACTGTCATTGAATGTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGTTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCTGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGTCTCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGt                                                            <  2:122099/275‑1 (MQ=255)
                                                                                                                                                                                                                   ggaacatttaTTGAATTTGAGCTGCCGCTACTGGAAGAGAAAGCAAGATCTACCCTGACTCAGGTTGTTATTTCCAACATGGACGAAATCAAAGAACTGTGCAAACTGTCCTGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGt                                                            <  3:191449/265‑1 (MQ=255)
                                                                                                                                                                                                                   gATACTGTCATTGAATTTGATCTGGCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGt                                                            <  1:322312/274‑1 (MQ=255)
                                                                                                                                                                                                                     tACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATTCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTAcgc                                                        >  2:55427/1‑276 (MQ=255)
                                                                                                                                                                                                                        tGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGctct                                                                                            >  1:346047/1‑237 (MQ=255)
                                                                                                                                                                                                                            aTTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCAtg                                                                                        <  3:346047/237‑1 (MQ=255)
                                                                                                                                                                                                                             ctGAATTTGATCTGTCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAAcccc                                                <  2:412145/275‑1 (MQ=255)
                                                                                                                                                                                                                                 aTTTGACCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGCCTCAGGTTGTTATCTCCAACATGGACCAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCt                                             <  1:337599/275‑1 (MQ=255)
                                                                                                                                                                                                                                   ttGATCTGCCTCTGCTGGAAGAGAAAGCCCAGTCTCCCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCg                                           <  3:605619/275‑1 (MQ=255)
                                                                                                                                                                                                                                    tGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTCACCGTGGGTTAAACCCAGGTTATCCGCATCAAGAAGTCATTCTTGCCGCAGTGAAAACTGGCGCCCATCCGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCCAACACAATTCATTCCTACCAGTCCTCTGGGTTTATCGCACCACTTCCAGCACACAAAACCCAGCCCgct                                         >  4:474653/1‑274 (MQ=255)
                                                                                                                                                                                                                                    tGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGACATTTTTTTATGCTTCCGCCAACGGCGGCAAAATCAATTCAACGCTCGCATGCTGCTGAGTGTAGCACATCACTTCCAGTACGCACAACCCCGCCCGGt                                         >  2:349568/1‑276 (MQ=255)
                                                                                                                                                                                                                                        cgtccGCGGCTGGAAGGGAAAGCCAAGGCTACCCTGAATCAGGTTGTTAGCTCCAACATGGACGAAAACAAAGAACTGATCAAACTTACCTGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTGGCCGAAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTg                                        <  3:142515/270‑1 (MQ=255)
                                                                                                                                                                                                                                          gCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACCCCCAACCCCCCTCGGTTCAc                                     >  3:428864/1‑274 (MQ=255)
                                                                                                                                                                                                                                           ccGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATAGCCCTCATGCTGCTGGTGGTAGCGCCGCACTTCCAATACGCTCACAcccgcccgcgtcact                                    >  3:552788/1‑267 (MQ=255)
                                                                                                                                                                                                                                                          aaaGCCAAGCCTACCCTGACTCAGGTTGTTATCGCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGcc                     <  1:428864/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                       cccTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCa        <  1:487313/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                        ccTGACTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCa                                      >  2:71613/1‑243 (MQ=255)
                                                                                                                                                                                                                                                                         cTGACTCAGGTTGTTATCTCCAACATGGGCGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGc     <  1:371131/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                            gcTCCGAGTGTTATGTCACACAAGGACGAAAGAAGAGAACAGCGCCGACTGGCAGGGTGCGAAACAGTGAGTTGAACCCCGGTGATCCGGATCAAGAAGTAATGCTTGCCGCAGTGAAACATGGCGCCCATCGTCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCa   <  4:110864/273‑1 (MQ=255)
                                                                                                                                                                                                                                                                            aCTCAGGTTGTTATCTCCAACATGGACGAAATCAACGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCGGCAGTGAAAAATGCCGCCCATCGGCGCAATTTTTTTATGCTTCCGCCAGCGGCAGCAACAGAAATTCATCCCCCGCATGATGCTGGGTGTAGCGATTGACTTCCCGGACGAGAAACCCCGCTAGGTCCACGGCTCACGTTCACGCCTCGCCTGTCACCAAACCAc  >  4:285938/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                            aCTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAACTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTCCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTACCTTTCAGCAAGCCa   >  1:633392/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                            aCTCAGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCAGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATTCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCCCTGCATCGGTTAACGCCTTCCCTTTCACCAAGcccc  >  4:258428/1‑273 (MQ=255)
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                               
TGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCAC  >  NC_000913/2535974‑2536516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: